ISPsy30
- Family Tn3
- Group
Isoform Synonym(s) TnPsy30
| Accession number | Transposition | Origin | Host |
|---|---|---|---|
| FR820585 | ND | Pseudomonas syringae | Pseudomonas syringae pv. Savastanoi Pseudomonas syringae pv. savastanoi NCPPB 3335 |
DNA section
IS Length : 5167 bp
Ends
IR Length : 36/38
IRL : GGGGTTGTAAGCCGGAATCCCAGAAATTTCCGTCACCCAGTGCCAAGTCC
IRR : GGGGTTGTAAGCCGGAACCCCAGAAAATTCCGTCACCCCAACTCCAGCCC
Insertion site
| Left flank | Direct repeat | Right flank | DR Length |
|---|---|---|---|
| TTACGTTTCT | AGTAGAGGCT | 0 |
DNA sequence
GGGGTTGTAAGCCGGAATCCCAGAAATTTCCGTCACCCAGTGCCAAGTCCAACCACGAGTACATCCAGGCGGCGGTCTCGACGAAAGCGACAGACCTTAT
GCATAGGAATTTCAAAATGCCTATGCATCAAGATGGAACTTTTTCTTGCCCGCTACCACCCATTTTTGTTTTGCAGGTTCAGTTTCCTGCAAGCCCGTAA
GCGGAGAAAATCAGCATATGGTGGGTATCAGCAGAGCAGGGTCATCTGGCGCTTATTTTGGTGGGTACACAGATCCGCCACGAATGCATCTAGGCGGCGG
TTTAGGCGAGAGCGAAGACTTGACAGCGCAACTATTTCTGCATCGAAATTCACTATGAGCTACAGATGATCTAAGAGTTTAACCCTATTCTAAAGAGATA
ATTGTTATGGGACTATGTATTTCAAGACCGTCTAGTAGTAGCTATCGTGAAAACTTGGAAACTTCTGCAAGTTCTCCGAACACCAGGCCTACTTTTGGTC
ATCAACCCGCGTCTACGAGCTACAGAGCATCAGACGAGATGAATGAACGTCCTGCAAAGTTTTCTCATTTTCAACTTGCACGACGTGGTGGAAATTATAC
CCTTAAAATGGTTTCTTTAGACGCTTATCAAGCAGAAAGACGGCACAGTGGCAATGCAATAAAGGATCGCAGTGAATCTACATTCCCGTGGGTCCGTGTC
TATCACTCTAAAACAGGTTTGGATTACAGCTTTCAGATCGACAGAACCACTACTGTCAAAGTGGCGGGGTTCAACGGCCTTACACCCAACGACGAAGGGA
CTCGGCATCTATACAGTGCTGGCACGAGTCAAATAAACATGCCTGTTGTCACAGATAACATGACTGCTTGTATTGCTGTTGCTTGTGCTGCAGAAAACGT
GGATGCTGACACGGGTGAACGTATGCGGGGCGCGCAGGTCCGCGTATTCCATCTGCTCCCTTTTTGTCACGAAGACCTTGTGCCAGAAGAGGTTTTAGCG
TCTATACGCGATTATCTGCAAAATGCCAGAGCACAGGGTCTGACAATGCGTGTCGCCATGCATGGTGGGGATAGAGAGGGTGATTTTTCAGTTAGCACGG
CGGATGCATTGAAACAGCTATTTGCAGATGAAGGGATTCCTCTAGAGTTCGACGAGACCTGCGCAAACAGAACTTCTGACACTCTGCTGGGCGCTGTTAT
TTTAGATGACAACTCGACTCACTTTATAAAACATTTGGTGACTGGCTAACTAAACCAACACTAGGTGTAACCTTAAAATGAAAAATCCCCAACCTGCAAT
CAGCGCGGTTTTTGACCAGGATTAATTGCGACTGACCAGATGGGGCAACCAGCATTAACTGCGAATAAACCTGTATTGAACCGCGCTGAAGCGGTAAATC
CAACGATCGTATCACCGGCCACCATCTGGCGAATATGATCGTGCAGACGCTCTAGAAATGTGCGGAAAACTCTGTCGCATTCCGCTACCATACGGAAACC
TATTCTTGATGGTTATTCGTCTATGTTGGTTGGGTACATGCGCGTGTCGTCGGACTCAGACAGGCAAAACACTGACTTACAACGCGATGCGCTGCTCAGT
GCCGGCATCGATGCACGACACCTGTTTGAGGATTACGTCTCCGGCACGAAGGATAACCGCACGGGTTTGGCTCAAGCGCTCCAATTCGTTCGCCCCGGCG
ACGTACTTGTGGTCTGGAAGCTGGATCGGCTCGGTCGCTCGCTGTCGCATCTGCTCAGCATCGTGACCTCGCTCCGGGACAAACAGGTGGCGTTCCGCTC
GCTGACCGAGGGCATGGATACCACCACGGCGTCTGGCGAGCTGCTTTTTCATGTGTTCGGTGCGCTCGCGCAATACGAACGCGCGCTGATCCAGGAGCGC
GTCGTCGCGGGTCTGGCCGCCGCCCGGCGGCGCGGTCGCATTGGCGGACGGCCGCAGGCGATCACCGGTGAGAAGCTGGATGCCATCGTTGCTGCGCTGA
ACGATGGCATCTCTAAGGCCGCCGTGTGCCGTAACTTCGGTGTTAAGCGCACCACCTTGATCGAAACCCTGGCGCGTGTCGGTAGGCAAGGTTACGGAAG
AGTTGCTGATGATCCATAAGAACAAGCTGATCAGTGTCCTGTCCGACGCCGAACAGGAAGCCCTATACGGGTTGCCGGATTTCGACGACGGTCAGCGACT
TGAATTTCTATCGTTATCGGAAACCGAACTGGCGTTCGCCGGCAATCGTCCCAGCCTACCTGCCCAAGTCTACTGCTTTTTGCAGATCGGTTACTTCAAG
GCCAAGCATGCCTTCTTCCGCTTCGACTGGAACGAGGTCGAGGACGATTGCACTTTCGTGTTGAGCCGCTATTTCCACGGCGAGACGTTCGAGCGCAAGC
CGATCACCAAACATGAGCATTACACCCAGCGCGAAGGGGTCTCCGAGCTGTTCGGCTATCGGCTTTGGACGGCCGACTTCCTGCCGCAGCTTGGGCAGCA
GGCCACGCAAGCCGCAAGGCGTGACATGACGCCAGGGTTCGTGGCCGCCGAGCTGATCGTCTGGCTCAACGAGCACAAGATCATCCGGCCCGGCTACACC
ACCCTGCAAGAGCTGGTCAGCGAAACACTGTCCGCTGAACGTCGGCGCTTGGGTGGCCTGCTGGCGCAAGTGCTGGACGACGCGGCCAAAAACGCGCTGG
CCCAGCTTCTGGTACGTGATGACACCCTGTCTCAACTAGCGGCGCTCAAGCAGGATGCCAAAAACTTCGGTTGGCGCCAGATGATCCGCGAGCGCGAGAA
GCGCGCCTTGCTGGAGCCGCTGCACCGAATCGCCAAGGCGCTGCTGCCCAAGCTGGGCGTTTCGCAGCAGAACCTGCTGTACTTCGCGAGCCTGGCAAAT
TTCTACACCGTCCACGATCTGCGCAATCTCAAGGCGGATCAGACCCAACTTTACCTACTGTGCTACGCCTGGCTGCGCTACCGGCAACTCTCCGACAATC
TGGTCGATGCAATGGCTTACCACATGAAGCAATTGGAGGAAGAGAGTAGCGCGGGTGCGCAGAAGTCTTTCGTCGCAGAGCAGGTGCGTCGTCAGCAGGA
CACACCGCAAGTAGGGCGCCTGCTGTTGATCTACGTCGATGACACGGTGGCCGATGCCACACCGTTCGGCGACGTGCGCCAGCGCGCGTACAAGATCATG
CCGAAAGATACGCTCCAACTCACCGGCCAGCGCATGAGCGTGAAGCCGGTGAGCAAGCTGTCCCTGCACTGGCAGGCAGTAGACGGCCTGGCCGAACGCA
ACCGCCGCCATTTGCGACCACTGTACGTCGCACTCGATTTTTCTGGCACTGTCCCCGACAGCCCTTGGCTAAAGGCGCTGGCCTGGGCCAAGAGTGTGTT
CGCCAAGCAACAGCGCCTGTCCCAACGGCCCCTAGCTGAATGTCCGGTGGCCACGTTGCCGAAACGGCTGCGGCCGTACCTGCTGACCTTCGATGCCGAC
GGCAAACCGACCGGTCTGCACGCCGACCGCTACGAGTTCTGGCTGTACCGGCAGATTAGGAAGCGTTTCAAGTCGGGGGAACTCTACCTCGACGACAGCC
TGCAACACCGCCACTTATCAGACGAGCTGGTCTCGATGAACGAGAAGGCCGACACGCTTGCGCAAATGGACATCCCCTTCCTACGGCAGCCGGTGGACGC
CCAGCTCGATGCGCTGACGCGGGAGCTGCGCGCGCAGTTGCTGGCATTCAACCGCGAGCTGAAGCGGGGCAAGCTGACGCACCTCACCTACGATAAGGAC
GCGAAGACGCTGATCTGGCACAAGCCCAAGGGCGAGAATCAGAAGGTGCGCGAGCAGTCGTTCTACGAGCAATTGCCGTACTGCGATGTTGCCGACGTAT
TCCGCTTCGTCAATGGTCAATGCCATTTTTTGTCCGCGTTGACGCCATTGCAGCCGCGCTATGCGAAGAAGGTGGCCGACGCCGATAGTTTGATGGCGGT
CGTCATCGCGCAGGCGATGAACCACGGCAATCAGGTCATGGCACGCACCAGCGACATTCCTTACCACGTGCTAGAGACGACCTATCAACAGTACCTGCGC
CAAGCATCGCTCCAGGCCGCCAACGACCGGATCAGCAATGCCATCGCCGCGTTGCCGATTTTCCCCCATTACTCGTTCGACCTGGATGCGTTGTACGGTG
CCGTCGATGGTCAGAAATTCAGCATCGAGCGCCCAACCGTGAAGGCGCGCCACTCGCGCAAATACTTTGGCCGTGGCAAGGGTGTCGTCGCCTACACGTT
GCTGTGTAACCACGTGCCGCTCAACGGCTATTTGATCGGCGCACACGACTTCGAGGCGCACCACGTGTTCGACATCTGGTATCGCAACACGTCGGACGTC
GTGCCGACCGCGATTACCGGTGATATGCACAGCGTCAATAAGGCCAACTTCGCCATCTTGCACTGGTTCGGTCCACGCTTTGAGCCGCGCTTCACCAATC
TCGAAGACCAACTCCAAGAATTGTACAGCGCCGATGATCCGGCGCTTTACGAGAAGTGCCTGATCCGGCCGGTCGGCCAGATCGATCAGCAGCTCATCAT
CAGTGAAAAACCGAACATCGACCAGATCGTTGCCACGCTAGGCCTCAAAGAGATGACGCAAGGCACGCTGATCCGCAAGCTTTGCACCTACACCGCGCCG
AATCCGACACGGCGCGCGATCTTCGAGTTCGACAAGCTCATCCGTAGCATCTACACGTTACGTTACCTGCGCGATCCGCAGTTGGAACGGAACGTACATC
GCTCGCAGAACCGCGTCGAGTCCTACCACCAGCTACGCTCGACCATCGCACAGGTCGGTGGAAAGAAGGAATTGACCGGGCGCACCGACATTGGAATCGA
GATCAGCAACCAGTGCGCTAGGCTGATCGCCAACGCCATCATCTACTACAACTCGGCCATCCTGTCGCACCTGCTGACGAAGTGCGAAGCGAGCGGCAAT
GCCAAGGCCGTGGCGCTGATCACCAAGATTTCACCGGCGGCCTGGCGGCACATTCTGCTGAATGGGCACTACACCTTCCAAAGCGACAAAATGATCGACC
TGGACGCACTACTGGCAGGGCTGGAGTTGGGGTGACGGAATTTTCTGGGGTTCCGGCTTACAACCCC
GCATAGGAATTTCAAAATGCCTATGCATCAAGATGGAACTTTTTCTTGCCCGCTACCACCCATTTTTGTTTTGCAGGTTCAGTTTCCTGCAAGCCCGTAA
GCGGAGAAAATCAGCATATGGTGGGTATCAGCAGAGCAGGGTCATCTGGCGCTTATTTTGGTGGGTACACAGATCCGCCACGAATGCATCTAGGCGGCGG
TTTAGGCGAGAGCGAAGACTTGACAGCGCAACTATTTCTGCATCGAAATTCACTATGAGCTACAGATGATCTAAGAGTTTAACCCTATTCTAAAGAGATA
ATTGTTATGGGACTATGTATTTCAAGACCGTCTAGTAGTAGCTATCGTGAAAACTTGGAAACTTCTGCAAGTTCTCCGAACACCAGGCCTACTTTTGGTC
ATCAACCCGCGTCTACGAGCTACAGAGCATCAGACGAGATGAATGAACGTCCTGCAAAGTTTTCTCATTTTCAACTTGCACGACGTGGTGGAAATTATAC
CCTTAAAATGGTTTCTTTAGACGCTTATCAAGCAGAAAGACGGCACAGTGGCAATGCAATAAAGGATCGCAGTGAATCTACATTCCCGTGGGTCCGTGTC
TATCACTCTAAAACAGGTTTGGATTACAGCTTTCAGATCGACAGAACCACTACTGTCAAAGTGGCGGGGTTCAACGGCCTTACACCCAACGACGAAGGGA
CTCGGCATCTATACAGTGCTGGCACGAGTCAAATAAACATGCCTGTTGTCACAGATAACATGACTGCTTGTATTGCTGTTGCTTGTGCTGCAGAAAACGT
GGATGCTGACACGGGTGAACGTATGCGGGGCGCGCAGGTCCGCGTATTCCATCTGCTCCCTTTTTGTCACGAAGACCTTGTGCCAGAAGAGGTTTTAGCG
TCTATACGCGATTATCTGCAAAATGCCAGAGCACAGGGTCTGACAATGCGTGTCGCCATGCATGGTGGGGATAGAGAGGGTGATTTTTCAGTTAGCACGG
CGGATGCATTGAAACAGCTATTTGCAGATGAAGGGATTCCTCTAGAGTTCGACGAGACCTGCGCAAACAGAACTTCTGACACTCTGCTGGGCGCTGTTAT
TTTAGATGACAACTCGACTCACTTTATAAAACATTTGGTGACTGGCTAACTAAACCAACACTAGGTGTAACCTTAAAATGAAAAATCCCCAACCTGCAAT
CAGCGCGGTTTTTGACCAGGATTAATTGCGACTGACCAGATGGGGCAACCAGCATTAACTGCGAATAAACCTGTATTGAACCGCGCTGAAGCGGTAAATC
CAACGATCGTATCACCGGCCACCATCTGGCGAATATGATCGTGCAGACGCTCTAGAAATGTGCGGAAAACTCTGTCGCATTCCGCTACCATACGGAAACC
TATTCTTGATGGTTATTCGTCTATGTTGGTTGGGTACATGCGCGTGTCGTCGGACTCAGACAGGCAAAACACTGACTTACAACGCGATGCGCTGCTCAGT
GCCGGCATCGATGCACGACACCTGTTTGAGGATTACGTCTCCGGCACGAAGGATAACCGCACGGGTTTGGCTCAAGCGCTCCAATTCGTTCGCCCCGGCG
ACGTACTTGTGGTCTGGAAGCTGGATCGGCTCGGTCGCTCGCTGTCGCATCTGCTCAGCATCGTGACCTCGCTCCGGGACAAACAGGTGGCGTTCCGCTC
GCTGACCGAGGGCATGGATACCACCACGGCGTCTGGCGAGCTGCTTTTTCATGTGTTCGGTGCGCTCGCGCAATACGAACGCGCGCTGATCCAGGAGCGC
GTCGTCGCGGGTCTGGCCGCCGCCCGGCGGCGCGGTCGCATTGGCGGACGGCCGCAGGCGATCACCGGTGAGAAGCTGGATGCCATCGTTGCTGCGCTGA
ACGATGGCATCTCTAAGGCCGCCGTGTGCCGTAACTTCGGTGTTAAGCGCACCACCTTGATCGAAACCCTGGCGCGTGTCGGTAGGCAAGGTTACGGAAG
AGTTGCTGATGATCCATAAGAACAAGCTGATCAGTGTCCTGTCCGACGCCGAACAGGAAGCCCTATACGGGTTGCCGGATTTCGACGACGGTCAGCGACT
TGAATTTCTATCGTTATCGGAAACCGAACTGGCGTTCGCCGGCAATCGTCCCAGCCTACCTGCCCAAGTCTACTGCTTTTTGCAGATCGGTTACTTCAAG
GCCAAGCATGCCTTCTTCCGCTTCGACTGGAACGAGGTCGAGGACGATTGCACTTTCGTGTTGAGCCGCTATTTCCACGGCGAGACGTTCGAGCGCAAGC
CGATCACCAAACATGAGCATTACACCCAGCGCGAAGGGGTCTCCGAGCTGTTCGGCTATCGGCTTTGGACGGCCGACTTCCTGCCGCAGCTTGGGCAGCA
GGCCACGCAAGCCGCAAGGCGTGACATGACGCCAGGGTTCGTGGCCGCCGAGCTGATCGTCTGGCTCAACGAGCACAAGATCATCCGGCCCGGCTACACC
ACCCTGCAAGAGCTGGTCAGCGAAACACTGTCCGCTGAACGTCGGCGCTTGGGTGGCCTGCTGGCGCAAGTGCTGGACGACGCGGCCAAAAACGCGCTGG
CCCAGCTTCTGGTACGTGATGACACCCTGTCTCAACTAGCGGCGCTCAAGCAGGATGCCAAAAACTTCGGTTGGCGCCAGATGATCCGCGAGCGCGAGAA
GCGCGCCTTGCTGGAGCCGCTGCACCGAATCGCCAAGGCGCTGCTGCCCAAGCTGGGCGTTTCGCAGCAGAACCTGCTGTACTTCGCGAGCCTGGCAAAT
TTCTACACCGTCCACGATCTGCGCAATCTCAAGGCGGATCAGACCCAACTTTACCTACTGTGCTACGCCTGGCTGCGCTACCGGCAACTCTCCGACAATC
TGGTCGATGCAATGGCTTACCACATGAAGCAATTGGAGGAAGAGAGTAGCGCGGGTGCGCAGAAGTCTTTCGTCGCAGAGCAGGTGCGTCGTCAGCAGGA
CACACCGCAAGTAGGGCGCCTGCTGTTGATCTACGTCGATGACACGGTGGCCGATGCCACACCGTTCGGCGACGTGCGCCAGCGCGCGTACAAGATCATG
CCGAAAGATACGCTCCAACTCACCGGCCAGCGCATGAGCGTGAAGCCGGTGAGCAAGCTGTCCCTGCACTGGCAGGCAGTAGACGGCCTGGCCGAACGCA
ACCGCCGCCATTTGCGACCACTGTACGTCGCACTCGATTTTTCTGGCACTGTCCCCGACAGCCCTTGGCTAAAGGCGCTGGCCTGGGCCAAGAGTGTGTT
CGCCAAGCAACAGCGCCTGTCCCAACGGCCCCTAGCTGAATGTCCGGTGGCCACGTTGCCGAAACGGCTGCGGCCGTACCTGCTGACCTTCGATGCCGAC
GGCAAACCGACCGGTCTGCACGCCGACCGCTACGAGTTCTGGCTGTACCGGCAGATTAGGAAGCGTTTCAAGTCGGGGGAACTCTACCTCGACGACAGCC
TGCAACACCGCCACTTATCAGACGAGCTGGTCTCGATGAACGAGAAGGCCGACACGCTTGCGCAAATGGACATCCCCTTCCTACGGCAGCCGGTGGACGC
CCAGCTCGATGCGCTGACGCGGGAGCTGCGCGCGCAGTTGCTGGCATTCAACCGCGAGCTGAAGCGGGGCAAGCTGACGCACCTCACCTACGATAAGGAC
GCGAAGACGCTGATCTGGCACAAGCCCAAGGGCGAGAATCAGAAGGTGCGCGAGCAGTCGTTCTACGAGCAATTGCCGTACTGCGATGTTGCCGACGTAT
TCCGCTTCGTCAATGGTCAATGCCATTTTTTGTCCGCGTTGACGCCATTGCAGCCGCGCTATGCGAAGAAGGTGGCCGACGCCGATAGTTTGATGGCGGT
CGTCATCGCGCAGGCGATGAACCACGGCAATCAGGTCATGGCACGCACCAGCGACATTCCTTACCACGTGCTAGAGACGACCTATCAACAGTACCTGCGC
CAAGCATCGCTCCAGGCCGCCAACGACCGGATCAGCAATGCCATCGCCGCGTTGCCGATTTTCCCCCATTACTCGTTCGACCTGGATGCGTTGTACGGTG
CCGTCGATGGTCAGAAATTCAGCATCGAGCGCCCAACCGTGAAGGCGCGCCACTCGCGCAAATACTTTGGCCGTGGCAAGGGTGTCGTCGCCTACACGTT
GCTGTGTAACCACGTGCCGCTCAACGGCTATTTGATCGGCGCACACGACTTCGAGGCGCACCACGTGTTCGACATCTGGTATCGCAACACGTCGGACGTC
GTGCCGACCGCGATTACCGGTGATATGCACAGCGTCAATAAGGCCAACTTCGCCATCTTGCACTGGTTCGGTCCACGCTTTGAGCCGCGCTTCACCAATC
TCGAAGACCAACTCCAAGAATTGTACAGCGCCGATGATCCGGCGCTTTACGAGAAGTGCCTGATCCGGCCGGTCGGCCAGATCGATCAGCAGCTCATCAT
CAGTGAAAAACCGAACATCGACCAGATCGTTGCCACGCTAGGCCTCAAAGAGATGACGCAAGGCACGCTGATCCGCAAGCTTTGCACCTACACCGCGCCG
AATCCGACACGGCGCGCGATCTTCGAGTTCGACAAGCTCATCCGTAGCATCTACACGTTACGTTACCTGCGCGATCCGCAGTTGGAACGGAACGTACATC
GCTCGCAGAACCGCGTCGAGTCCTACCACCAGCTACGCTCGACCATCGCACAGGTCGGTGGAAAGAAGGAATTGACCGGGCGCACCGACATTGGAATCGA
GATCAGCAACCAGTGCGCTAGGCTGATCGCCAACGCCATCATCTACTACAACTCGGCCATCCTGTCGCACCTGCTGACGAAGTGCGAAGCGAGCGGCAAT
GCCAAGGCCGTGGCGCTGATCACCAAGATTTCACCGGCGGCCTGGCGGCACATTCTGCTGAATGGGCACTACACCTTCCAAAGCGACAAAATGATCGACC
TGGACGCACTACTGGCAGGGCTGGAGTTGGGGTGACGGAATTTTCTGGGGTTCCGGCTTACAACCCC
Protein section
ORF number : 3
ORF 1
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 843 bp | 280 aa | 407 | 1249 | + | No |
Annotation : Description :
ORF sequence :
MGLCISRPSSSSYRENLETSASSPNTRPTFGHQPASTSYRASDEMNERPAKFSHFQLARRGGNYTLKMVSLDAYQAERRHSGNAIKDRSESTFPWVRVYH
SKTGLDYSFQIDRTTTVKVAGFNGLTPNDEGTRHLYSAGTSQINMPVVTDNMTACIAVACAAENVDADTGERMRGAQVRVFHLLPFCHEDLVPEEVLASI
RDYLQNARAQGLTMRVAMHGGDREGDFSVSTADALKQLFADEGIPLEFDETCANRTSDTLLGAVILDDNSTHFIKHLVTG
SKTGLDYSFQIDRTTTVKVAGFNGLTPNDEGTRHLYSAGTSQINMPVVTDNMTACIAVACAAENVDADTGERMRGAQVRVFHLLPFCHEDLVPEEVLASI
RDYLQNARAQGLTMRVAMHGGDREGDFSVSTADALKQLFADEGIPLEFDETCANRTSDTLLGAVILDDNSTHFIKHLVTG
Blast result :ORF 2
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 597 bp | 198 aa | 1523 | 2119 | + | No |
AG : Tn3 resolvase
ORF sequence :
MLVGYMRVSSDSDRQNTDLQRDALLSAGIDARHLFEDYVSGTKDNRTGLAQALQFVRPGDVLVVWKLDRLGRSLSHLLSIVTSLRDKQVAFRSLTEGMDT
TTASGELLFHVFGALAQYERALIQERVVAGLAAARRRGRIGGRPQAITGEKLDAIVAALNDGISKAAVCRNFGVKRTTLIETLARVGRQGYGRVADDP
TTASGELLFHVFGALAQYERALIQERVVAGLAAARRRGRIGGRPQAITGEKLDAIVAALNDGISKAAVCRNFGVKRTTLIETLARVGRQGYGRVADDP
Blast result :ORF 3
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 3027 bp | 1008 aa | 2109 | 5135 | + | No |
Chemistry : DDE
ORF sequence :
MIHKNKLISVLSDAEQEALYGLPDFDDGQRLEFLSLSETELAFAGNRPSLPAQVYCFLQIGYFKAKHAFFRFDWNEVEDDCTFVLSRYFHGETFERKPIT
KHEHYTQREGVSELFGYRLWTADFLPQLGQQATQAARRDMTPGFVAAELIVWLNEHKIIRPGYTTLQELVSETLSAERRRLGGLLAQVLDDAAKNALAQL
LVRDDTLSQLAALKQDAKNFGWRQMIREREKRALLEPLHRIAKALLPKLGVSQQNLLYFASLANFYTVHDLRNLKADQTQLYLLCYAWLRYRQLSDNLVD
AMAYHMKQLEEESSAGAQKSFVAEQVRRQQDTPQVGRLLLIYVDDTVADATPFGDVRQRAYKIMPKDTLQLTGQRMSVKPVSKLSLHWQAVDGLAERNRR
HLRPLYVALDFSGTVPDSPWLKALAWAKSVFAKQQRLSQRPLAECPVATLPKRLRPYLLTFDADGKPTGLHADRYEFWLYRQIRKRFKSGELYLDDSLQH
RHLSDELVSMNEKADTLAQMDIPFLRQPVDAQLDALTRELRAQLLAFNRELKRGKLTHLTYDKDAKTLIWHKPKGENQKVREQSFYEQLPYCDVADVFRF
VNGQCHFLSALTPLQPRYAKKVADADSLMAVVIAQAMNHGNQVMARTSDIPYHVLETTYQQYLRQASLQAANDRISNAIAALPIFPHYSFDLDALYGAVD
GQKFSIERPTVKARHSRKYFGRGKGVVAYTLLCNHVPLNGYLIGAHDFEAHHVFDIWYRNTSDVVPTAITGDMHSVNKANFAILHWFGPRFEPRFTNLED
QLQELYSADDPALYEKCLIRPVGQIDQQLIISEKPNIDQIVATLGLKEMTQGTLIRKLCTYTAPNPTRRAIFEFDKLIRSIYTLRYLRDPQLERNVHRSQ
NRVESYHQLRSTIAQVGGKKELTGRTDIGIEISNQCARLIANAIIYYNSAILSHLLTKCEASGNAKAVALITKISPAAWRHILLNGHYTFQSDKMIDLDA
LLAGLELG
KHEHYTQREGVSELFGYRLWTADFLPQLGQQATQAARRDMTPGFVAAELIVWLNEHKIIRPGYTTLQELVSETLSAERRRLGGLLAQVLDDAAKNALAQL
LVRDDTLSQLAALKQDAKNFGWRQMIREREKRALLEPLHRIAKALLPKLGVSQQNLLYFASLANFYTVHDLRNLKADQTQLYLLCYAWLRYRQLSDNLVD
AMAYHMKQLEEESSAGAQKSFVAEQVRRQQDTPQVGRLLLIYVDDTVADATPFGDVRQRAYKIMPKDTLQLTGQRMSVKPVSKLSLHWQAVDGLAERNRR
HLRPLYVALDFSGTVPDSPWLKALAWAKSVFAKQQRLSQRPLAECPVATLPKRLRPYLLTFDADGKPTGLHADRYEFWLYRQIRKRFKSGELYLDDSLQH
RHLSDELVSMNEKADTLAQMDIPFLRQPVDAQLDALTRELRAQLLAFNRELKRGKLTHLTYDKDAKTLIWHKPKGENQKVREQSFYEQLPYCDVADVFRF
VNGQCHFLSALTPLQPRYAKKVADADSLMAVVIAQAMNHGNQVMARTSDIPYHVLETTYQQYLRQASLQAANDRISNAIAALPIFPHYSFDLDALYGAVD
GQKFSIERPTVKARHSRKYFGRGKGVVAYTLLCNHVPLNGYLIGAHDFEAHHVFDIWYRNTSDVVPTAITGDMHSVNKANFAILHWFGPRFEPRFTNLED
QLQELYSADDPALYEKCLIRPVGQIDQQLIISEKPNIDQIVATLGLKEMTQGTLIRKLCTYTAPNPTRRAIFEFDKLIRSIYTLRYLRDPQLERNVHRSQ
NRVESYHQLRSTIAQVGGKKELTGRTDIGIEISNQCARLIANAIIYYNSAILSHLLTKCEASGNAKAVALITKISPAAWRHILLNGHYTFQSDKMIDLDA
LLAGLELG
Blast result :
Comments
It is homologous to a Tn5501-like element present in two IncP-1 beta plasmids (accession no. AJ863570 and EF628291); they have a conserved 3.7 kb 3' fragment and a variable configuration of the 5' end. This copy of ISPsy30 in strain NCPPB 3335 has entrapped the Type III secretion system effector gene hopAF1 (ORF1 : passenger gene).
ISPsy30 is 77% (ORF2 : the resolvase) aa similar to ISMpo10 and 49% (ORF3 : the transposase) to ISShfr9.
ISPsy30 is 77% (ORF2 : the resolvase) aa similar to ISMpo10 and 49% (ORF3 : the transposase) to ISShfr9.
References
1] Jesus Murillo (2011) Direct submission