ISAtsp7
- Family IS630
- Group
Isoform Synonym(s)
| Accession number | Transposition | Origin | Host |
|---|---|---|---|
| ND | Arthrospira sp. | Arthrospira sp. |
DNA section
IS Length : 1032 bp
Ends
IR Length : 21/30
IRL : CAGCCTTTACACAAAACACAAAGTACGGTATAATGTATGGCTATTGTATA
IRR : CAGCCTTTATCTTAATCATTAAGCACAGTAACAACACTTGGTAAACCAGT
Insertion site
| Left flank | Direct repeat | Right flank | DR Length |
|---|---|---|---|
| AATCAGGCAT | TA | CAACCAATTG | 2 |
DNA sequence
CAGCCTTTACACAAAACACAAAGTACGGTATAATGTATGGCTATTGTATAAGTGATCACAACCAACTACCATGAGCCGTTACTCTTTAGATTTTCGGAAA
AAGATAGTAGAAGCCTATGAAAAAGGAGACACTTCTATCCGAAAAGTAGCGAAACGCTTTTTGGTGAGTCCAGACACGGTAAGGCGACTGGTCAAACAGT
ATCGATTAACGGGAGACTTATCTCCTCGTAAGTGTGGCACTAAAAAGAAAAGCATTTTATCTAAGCATGAGGAAGCCGTGATAGATATTGTAGAAGCCCA
CCCCGACCTGACCTTATGGCAATATAGTGATAAGCTCAGAGACAAGCTGGGCATAAATGTCAGTACGACCATGATAGATAGATTTTTAAAGCAGCACGAT
ATAAGTCTCAAAAAAAAACATACAGGAGCGAAAAAGTAGTAACAGAAGAAGTACAGAAAGCGCGAGTAGATTATTGGGAAAGAATTAGAGATGTGGCTCG
TGAAAAGCTTGTGTTTATTGATGAAAGTGGGTTATGGGTAGGGATGAGCAGACCGTTAGCCAGAGCGACGAAAGGGAAAAAAGTCTATGAACTGCGGAAA
TCCTATCGAGGTCAAAAAATGACAATAATTGGTGCAATTAAGCTCTCCGGAGGGGTGGCGACTCAAACATTAGAAGGGTCCATGAAAAAAGAGGATTTTC
TCCAATTCATCAAGTTAGATTTATTGCCAAAATTAAAAAAAGGAGATGTAGTGGTAATGGATAACTGAAATTCTCATCATCGAGAAGAAGTCAAAGAAAT
GATAGAGTCAGTAGGAGCCAGGGTAGAATATCTGCCGGTGTATTCCCCTGAGTTTAACCCGATAGAGATGATGTGGTCACAGCTCAAAAGTTTAGTGTGC
AAGTTCAGGACGGAGACCATGGAATTATTAGTGAGATTGGTAGAAGTGGCGGTAAGCCTTGTGGATTTACAGTGTTTGAATAACTGGTTTACCAAGTGTT
GTTACTGTGCTTAATGATTAAGATAAAGGCTG
AAGATAGTAGAAGCCTATGAAAAAGGAGACACTTCTATCCGAAAAGTAGCGAAACGCTTTTTGGTGAGTCCAGACACGGTAAGGCGACTGGTCAAACAGT
ATCGATTAACGGGAGACTTATCTCCTCGTAAGTGTGGCACTAAAAAGAAAAGCATTTTATCTAAGCATGAGGAAGCCGTGATAGATATTGTAGAAGCCCA
CCCCGACCTGACCTTATGGCAATATAGTGATAAGCTCAGAGACAAGCTGGGCATAAATGTCAGTACGACCATGATAGATAGATTTTTAAAGCAGCACGAT
ATAAGTCTCAAAAAAAAACATACAGGAGCGAAAAAGTAGTAACAGAAGAAGTACAGAAAGCGCGAGTAGATTATTGGGAAAGAATTAGAGATGTGGCTCG
TGAAAAGCTTGTGTTTATTGATGAAAGTGGGTTATGGGTAGGGATGAGCAGACCGTTAGCCAGAGCGACGAAAGGGAAAAAAGTCTATGAACTGCGGAAA
TCCTATCGAGGTCAAAAAATGACAATAATTGGTGCAATTAAGCTCTCCGGAGGGGTGGCGACTCAAACATTAGAAGGGTCCATGAAAAAAGAGGATTTTC
TCCAATTCATCAAGTTAGATTTATTGCCAAAATTAAAAAAAGGAGATGTAGTGGTAATGGATAACTGAAATTCTCATCATCGAGAAGAAGTCAAAGAAAT
GATAGAGTCAGTAGGAGCCAGGGTAGAATATCTGCCGGTGTATTCCCCTGAGTTTAACCCGATAGAGATGATGTGGTCACAGCTCAAAAGTTTAGTGTGC
AAGTTCAGGACGGAGACCATGGAATTATTAGTGAGATTGGTAGAAGTGGCGGTAAGCCTTGTGGATTTACAGTGTTTGAATAACTGGTTTACCAAGTGTT
GTTACTGTGCTTAATGATTAAGATAAAGGCTG
Recoding section
- Recoding by frameshift
- Frame
- Type
- Experimentally demonstrated
Stimulators :
- Shine-Dalgarno sequence :
- Secondary structure :
Recoding motif :
Protein section
ORF number : 3
ORF 1
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 369 bp | 122 aa | 71 | 439 | + | No |
Description : First part of the transposase
ORF sequence :
MSRYSLDFRKKIVEAYEKGDTSIRKVAKRFLVSPDTVRRLVKQYRLTGDLSPRKCGTKKKSILSKHEEAVIDIVEAHPDLTLWQYSDKLRDKLGINVSTT
MIDRFLKQHDISLKKKHTGAKK
MIDRFLKQHDISLKKKHTGAKK
Blast result :ORF 2
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 633 bp | 210 aa | 382 | 1014 | + | No |
Description : Second part of the transposase
ORF sequence :
IFKAARYKSQKKTYRSEKVVTEEVQKARVDYWERIRDVAREKLVFIDESGLWVGMSRPLARATKGKKVYELRKSYRGQKMTIIGAIKLSGGVATQTLEGS
MKKEDFLQFIKLDLLPKLKKGDVVVMDN*NSHHREEVKEMIESVGARVEYLPVYSPEFNPIEMMWSQLKSLVCKFRTETMELLVRLVEVAVSLVDLQCLN
NWFTKCCYCA
MKKEDFLQFIKLDLLPKLKKGDVVVMDN*NSHHREEVKEMIESVGARVEYLPVYSPEFNPIEMMWSQLKSLVCKFRTETMELLVRLVEVAVSLVDLQCLN
NWFTKCCYCA
Blast result :ORF 3
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 944 bp | 315 aa | 71 | 1014 | + | Yes |
Chemistry : DDE
ORF sequence :
MSRYSLDFRKKIVEAYEKGDTSIRKVAKRFLVSPDTVRRLVKQYRLTGDLSPRKCGTKKKSILSKHEEAVIDIVEAHPDLTLWQYSDKLRDKLGINVSTT
MIDRFLKQHDISLKKKTYRSEKVVTEEVQKARVDYWERIRDVAREKLVFIDESGLWVGMSRPLARATKGKKVYELRKSYRGQKMTIIGAIKLSGGVATQT
LEGSMKKEDFLQFIKLDLLPKLKKGDVVVMDN*NSHHREEVKEMIESVGARVEYLPVYSPEFNPIEMMWSQLKSLVCKFRTETMELLVRLVEVAVSLVDL
QCLNNWFTKCCYCA*
MIDRFLKQHDISLKKKTYRSEKVVTEEVQKARVDYWERIRDVAREKLVFIDESGLWVGMSRPLARATKGKKVYELRKSYRGQKMTIIGAIKLSGGVATQT
LEGSMKKEDFLQFIKLDLLPKLKKGDVVVMDN*NSHHREEVKEMIESVGARVEYLPVYSPEFNPIEMMWSQLKSLVCKFRTETMELLVRLVEVAVSLVDL
QCLNNWFTKCCYCA*
Blast result :
Comments
ISAtsp7 is 69% aa similar to ISSoc15. The third ORF is a putative ORFAB transposase reconstructed in silico by possible -1 frameshift.
There are STOP codon in frame and the transposase is probably not active.
There are STOP codon in frame and the transposase is probably not active.
References
1] ISfinder annotation (2007)