ISAtsp6

  • Family IS1
  • Group
MGE type ISRelated element(s) :
Isoform Synonym(s)
Accession numberTranspositionOriginHost
ND Arthrospira sp.
Arthrospira sp. PCC 8005
DNA section
IS Length : 745 bp

Ends


IR Length : 16/23

IRL : GGTGTTGCATAATAGAAAATGATATTCTCAAATAAATCAAGATGGATTGT
IRR : GGTATTGCACAGTGAGGAATGATTAAGAGAACCGAGAAAGTTGTCCATGC

Insertion site


Left flankDirect repeatRight flankDR Length
CTCAAGTTATGTCTTCCTTC0
TCGTTTTTGCTAAAAGACAA0
TAGGAATACCCACAATCCAAGATAGAGCA9

DNA sequence

GGTGTTGCATAATAGAAAATGATATTCTCAAATAAATCAAGATGGATTGTCCTTATTGCCAAAGCCATAAAGTAGTCAAAAATGGTCATCGTCAGGGAAA
ACAGAGTTACCTGTGCCGTGAATGTGGTCGCCAATTTCGAGAAAATCCCTGTCCTGGAGGTTACAGTTCTGATGTCAAAGAGCTTTGTGTGAAAATGTCC
CTCAATGGCATGGGATTTCGAGCCATTGAGAGAGTCACTGGTATTTCTCACAACACAATACTTAACTGGGTTAGAGTTGCAGAAACCCACATTGATGAGG
AAAACTATGAAATTCCTGAAATAGCTCAAATTGATGAGCCTCAGACTTTTGTGGGTTCAAAAAAAAACCATCTGGGTCTGGACAGTTGTGAATACCAAAC
TGCCTGGAATTCTGAAGTTTGTCATAGGCGACAGCTCATTGCTGACTTTTACCACATTATGGCAAATGATTCAGGGCTGGGCTGGTTTTCTATATATTAC
GGACCGATACAAAGTTTATCCTTGCTTAATTGAGGATTGCAATCATCTAGTCAGCAAAACGGCTATGACAAGAGTAGAAGGAGAAAACTGCCGTCTGAGG
CACTATTTAGCCAGGCTACATCGCAAAACTTTGTGTGATTCCAAGTCCACTGAGATGTTGTACAAATCAATTCGCTTACTTATCTATTATTTGAAGCATG
GACAACTTTCTCGGTTCTCTTAATCATTCCTCACTGTGCAATACC
Recoding section
  • Recoding by frameshift
  • Frame
  • Type
  • Experimentally demonstrated

Stimulators :

  • Shine-Dalgarno sequence :
  • Secondary structure :

Recoding motif :

Protein section
ORF number : 3

 

ORF 1
LengthBeginEndStrandFusion ORF
492 bp163 aa42533+No
ORF function : Transposase
Description : First part of the transposase

ORF sequence :

MDCPYCQSHKVVKNGHRQGKQSYLCRECGRQFRENPCPGGYSSDVKELCVKMSLNGMGFRAIERVTGISHNTILNWVRVAETHIDEENYEIPEIAQIDEP
QTFVGSKKNHLGLDSCEYQTAWNSEVCHRRQLIADFYHIMANDSGLGWFSIYYGPIQSLSLLN

 

Blast result :
ORF 2
LengthBeginEndStrandFusion ORF
399 bp132 aa325723+No
ORF function : Transposase
Description : Second part of the transposase

ORF sequence :

LKLMSLRLLWVQKKTIWVWTVVNTKLPGILKFVIGDSSLLTFTTLWQMIQGWAGFLYITDRYKVYPCLIEDCNHLVSKTAMTRVEGENCRLRHYLARLHR
KTLCDSKSTEMLYKSIRLLIYYLKHGQLSRFS

 

Blast result :
ORF 3
LengthBeginEndStrandFusion ORF
682 bp226 aa42723+Yes
ORF function : Transposase
Chemistry : DDE

ORF sequence :

MDCPYCQSHKVVKNGHRQGKQSYLCRECGRQFRENPCPGGYSSDVKELCVKMSLNGMGFRAIERVTGISHNTILNWVRVAETHIDEENYEIPEIAQIDEP
QTFVGSKKTIWVWTVVNTKLPGILKFVIGDSSLLTFTTLWQMIQGWAGFLYITDRYKVYPCLIEDCNHLVSKTAMTRVEGENCRLRHYLARLHRKTLCDS
KSTEMLYKSIRLLIYYLKHGQLSRFS

 

Blast result :
Comments
ISAtsp6 is 71% aa similar to ISMae3. The third ORF is a putative ORFAB transposase reconstructed in silico by possible -1 frameshift.
References
1] ISfinder annotation (2009)
2] Morin Nicolas (2009 Direct submission