ISAtsp5

  • Family IS4
  • Group IS10
MGE type ISRelated element(s) :
Isoform Synonym(s)
Accession numberTranspositionOriginHost
ND Arthrospira sp.
Arthrospira sp. PCC 8005
DNA section
IS Length : 1320 bp

Ends


IR Length :

IRL : CTGAGAGGGTGACAACGGAGGTCAAACCCAGACAGGGCAATTGTTTGAGA
IRR : CTGAGAGGGTGACAAGAGAGCTAGACTGATTGCTGTACAAGGGATAGAGC

Insertion site


Left flankDirect repeatRight flankDR Length
GGAGGTTACAGTTCTGATGTCAAAGAGCT9
GCAGCTCAATGCCGTTTACGCCGTTTACT9

DNA sequence

CTGAGAGGGTGACAACGGAGGTCAAACCCAGACAGGGCAATTGTTTGAGATAATGGAGATATCGTCAAAAAGCGGGTGGCCTTGGGAGCCACCCTGACAA
AAAATGCTAAGGACATTATACCAAAAACTATTACGAATCAATTTGAGCGAGAGTCAAGCACAGACGTTAGAACTCTTAGTGTTGATGCTTCAAAGTTATC
GGCAAGTCAGATTATCAACCCTCGCCAACGTTTTTCCACAACCCATTCAATACAGTAGTCGCCTCCGAAATATCCAACGATTTCTGAAACTCCCCCAACT
TTCGGCTAAGTTGCTGTGGTTCCCAATAATCAAGGCCGCTCTCAAATCTGAATTTAGAGAAAAACATTTAAATAGAGAGCAACGGAGAAAGCGCTCAAAA
TTCAGATTGAAAACCAAAAACTATGTAGCGGTTGCTCTCGACCGCACTCAATGGAGAGACCGAAATCTCCTGATGGTCACGATAATTTGGGGACATCATG
CTTTACCAATATATTGGGAGCTACTCCCAAAGTTAGGCAGTAGCTCTTTCCGGGAACAGAAACGGGTATTAGGGCCGGTGTTGGCTCTTTTAAAACCTTA
TCCGGTTGTAGTGATAGGAGACCGTGAGTTTCATAGTGCTCAACTCGCGGACTGGCTCAGAGTAAGGGGTGTAAATGTTGTATTTCGTCAGAAAAAGAGT
GCTTTTGTAGCTACCTCATGCCAGCCAGGTAAGTCTTTAAAAACTCAAGGATTTAAGTCGGGCGAATCCCATTTTTTTAAAAATGTAACCTTGCAAAAAT
TTGCACCTATTCATGGATTTAACCTAGGAGTTTATTGGCAAAAAATTCACCGAGGAAAAAAGGTAAAAAAACCTTGGTATTTACTAACTACGCTTGATAA
CCCCAAGCTGGTTAAGCAACTTTAGCAAGCGCGGTGGGGTGTCGAAATGATGTTTAGAGATTGTCAAAGTGGGGGCTATAATATGGAATCTAATCGGGTG
GATTCAACCCGATTTTTAGCCTTGGTTTTGTTGATTACTTTTGCTTATTGGCTGGCGACACTAGGAGGTCATGAGTGGGAAGCTAATCACTTAGTAGCTT
ACCTCGGTCGAAGCGAAAAAACTCCTAATAATTTTCCCCATCACAGTATTTTTGGGCTCAGATTATCGGGTTATGCTTGGAGCCAGTCGCTAGTTTTCTG
GCAAGAAGAGATGTTAGCATTAATGGCCCTCAAGCCTCATAAAGCTCAGAATTTTCGGCAAGGACTAAATGCTCTATCCCTTGTACAGCAATCAGTCTAG
CTCTCTTGTCACCCTCTCAG
Protein section
ORF number : 1

 

ORF 1
LengthBeginEndStrandFusion ORF
1197 bp398 aa1041300+No
ORF function : Transposase
Chemistry : DDE

ORF sequence :

MLRTLYQKLLRINLSESQAQTLELLVLMLQSYRQVRLSTLANVFPQPIQYSSRLRNIQRFLKLPQLSAKLLWFPIIKAALKSEFREKHLNREQRRKRSKF
RLKTKNYVAVALDRTQWRDRNLLMVTIIWGHHALPIYWELLPKLGSSSFREQKRVLGPVLALLKPYPVVVIGDREFHSAQLADWLRVRGVNVVFRQKKSA
FVATSCQPGKSLKTQGFKSGESHFFKNVTLQKFAPIHGFNLGVYWQKIHRGKKVKKPWYLLTTLDNPKLVKQL*QARWGVEMMFRDCQSGGYNMESNRVD
STRFLALVLLITFAYWLATLGGHEWEANHLVAYLGRSEKTPNNFPHHSIFGLRLSGYAWSQSLVFWQEEMLALMALKPHKAQNFRQGLNALSLVQQSV

 

Blast result :
Comments
ISAtsp5 is 66% aa similar to ISAcma19. There is a STOP codon in frame.
References
1] ISfinder annotation (2009)
2] Morin Nicolas (2009) Direct submission