ISfinder Mirror Site

ISAtsp1

Family IS630
Group

MGE type ISRelated element(s) :
Isoform Synonym(s)

Accession number	Transposition	Origin	Host
	ND	Arthrospira sp.	Arthrospira sp. PCC 8005

DNA section

IS Length : 957 bp

Ends

IR Length : 22/37

IRL : TAGCACAAGACAGAACACTTAGGACGTATAATGGAGAGGACGAGATGACT
IRR : TAGGATTAGTCAAGGTGGTTAGGACGCAGCTTTGAGAACGGAATCCATGG

Insertion site

Left flank	Direct repeat	Right flank	DR Length
GTGCTATATC	TA	AATGGTCTGA	2
CCGCAGTATC	TA	TATCCCCACC	2
GCCTGGCTTT	TA	GCTTACCTCG	2

DNA sequence

TAGCACAAGACAGAACACTTAGGACGTATAATGGAGAGGACGAGATGACTAAGAAGACCAAAAATGCCAGCCCCCTATAGTTACGACCTCAGACAAAAAG
TTATTGATGCCATTGAACTAGACGGTATGCCCAAAACAGAAGCCAGTCAAGTTTTCCATGTCAGCCGGAACACCATTAATCTCTGGCTGCAAAGAAAAGC
ACAGACCGGAGACTTCCTCCCTAAACCTCATCACCGACCTGGCAATAACCACAAAATTACCGACTGGCAAAAATTCAAGGCTTTTGCCCAAGAGCATGGC
GACAAAACAGCAGCTCAAATGGCTGAACTTTGGGATGACGACATCTCTCCTCGCACCATATCCAGAGCCTTGAAGAAAATTGGCTTCACCAGAAAAAAAA
CTTACGGCTACCAAGAACGTGATGAGCAACAGCGAGAGGAGTTTATGGCTCAGATTGAACAGATGGAGCCGGAAGAAGTGGTCTACCTCGATGAAGCCGG
CATGAATAGTCAGGACTCGGATTACCCTTATGGTTACTGCGAGGAAGGAAAACGCTTCCATGCACTCAAATCAGGGAAGAGGCAGGGCAGGGTAAGTATG
ATAGCCGCATGGTGTCATCAACAACTCTTAGCTCCCTTTAGCTTTGAGGGTTGTTGTCATCGGACAGTGTTTGAGTTGTGGTTGGAGTTCATCTTAATTC
CAACATTGAAGCCAGGTCAGACTCTAGTATTGGACAATGCAACGTTTCATAAAGGGGGGCGGATTGCTGAACTGGTGGAGGCAGCTCAATGCCGTTTACT
CTATCTACCACCTTATTCGCCAGACCTCAACAAGATAGAGAAATGTTGGTCGTGGCTGAAAGCCCGTATTCGCCACTGCATTGAGCAGTTTGATTCTCTC
CATGATGCCATGGATTCCGTTCTCAAAGCTGCGTCCTAACCACCTTGACTAATCCTA

Protein section

ORF number : 1

ORF 1

Length		Begin	End	Strand	Fusion ORF
876 bp	291 aa	64	939	+	No

ORF function : Transposase
Chemistry : DDE

ORF sequence :

MPAPYSYDLRQKVIDAIELDGMPKTEASQVFHVSRNTINLWLQRKAQTGDFLPKPHHRPGNNHKITDWQKFKAFAQEHGDKTAAQMAELWDDDISPRTIS
RALKKIGFTRKKTYGYQERDEQQREEFMAQIEQMEPEEVVYLDEAGMNSQDSDYPYGYCEEGKRFHALKSGKRQGRVSMIAAWCHQQLLAPFSFEGCCHR
TVFELWLEFILIPTLKPGQTLVLDNATFHKGGRIAELVEAAQCRLLYLPPYSPDLNKIEKCWSWLKARIRHCIEQFDSLHDAMDSVLKAAS

Blast result :

Comments

ISAtsp1 is 79% aa similar to ISAcma25.

References

1] ISfinder annotation (2009)
2] Morin Nicolas (2009) Direct submission.