ISAzs37

  • Family IS630
  • Group
MGE type ISRelated element(s) :
Isoform Synonym(s)
Accession numberTranspositionOriginHost
NC_013860 ND Azospirillum sp.
Azospirillum sp. B510 plasmid pAB510f
DNA section
IS Length : 1058 bp

Ends


IR Length : 18/24

IRL : GAGCGGTATTCGACCGGGTTGAATCGGTGTTTTTCTGTGGGATTCCCATT
IRR : GCGCGATTTTCGATCAGGTTGGATATCCCGCATTTTTGAAGAGGTTTCTG

Insertion site


Left flankDirect repeatRight flankDR Length
CTGTGGAATCGGCGTTAGCGCGCCCTCAGAACC2

DNA sequence

GAGCGGTATTCGACCGGGTTGAATCGGTGTTTTTCTGTGGGATTCCCATTTCTGGTAAAATCAGCGATTCTGAGCGGGTGATTAAGCGACCCGGAGCCGG
ACATGGCAGGCCCCTATTCTCAGGATCTTCGGGACCGCGTTGCCGCGGCGGTGTCGAGCGGCTGCTCGGCGCGCGCTGCGGCGGCGCGGTTCAGCATCAG
CGTCAGCACGGCGATCCGCTGGGCCAAACGCTTGGGCGCGGAAGGGCATGCCCAGGCGCGGCCAATGGGTGGCGACCACCGCTCGCGCCTGAGCGATCAC
CGTGAGGCGGTGCTGACGTTGGTCGCCCACCAGCCCGACCTGACGCTGGAGGAGATCCGCGCCGAACTGAACGCCCGCCATGGCGTGAGCGTCAGCCTCG
GCACGGTGTGGCGCTTCCTCAAGGCCCAGAACCTCACCCTTAAAAAAAACTCTGCACGCCGCCGAGCAGCAGCGTGATGACGTCGTCGAGGCCCGGCGCG
CCTTCATCCGCCGTCAGCCGGCCCTTGATCCCGAGCGCCTGGTGTTCCTTGATGAGACCTGGGCCAGCACCAACATGACCAGGCGCCACGGGCGGTGTGC
GCGCGGCTTGCGCCTCCTGGCTCCCGTCCCACACGGGCATTGGAAGACGACCACGCTGATCGCCGGCCTGCGCACCACCGGCATCGTCGCCCCCTATGTG
CTCGACGGGCCGATCAATGCCACCGTCTTCCGCGCCTACGTCGAGCAGGTTCTGGCCCCGACCCTGCAAAGTGGCGACATCCTCATCCTCGACAACCTGT
CCAGCCATAAGGTCGCCGGCGTCCGAGAAGCCGTCGAGGCGCGCGGCGCGCGGGTCCTCTACCTGCCGCCCTACAGCCCCGACCTCAACCCCATCGAGCA
AGCCTTCGCCAAGCTCAAGGCGCTCCTGCGAAAAGCGGCCGAGCGCTCCCGTGACGGACTCTGGAACGCCATCGGACGCATCATCGACATCTACAAGCCA
AACGAGTGCAGAAACCTCTTCAAAAATGCGGGATATCCAACCTGATCGAAAATCGCGC
Recoding section
  • Recoding by frameshift
  • Frame
  • Type
  • Experimentally demonstrated

Stimulators :

  • Shine-Dalgarno sequence :
  • Secondary structure :

Recoding motif :

Protein section
ORF number : 3

 

ORF 1
LengthBeginEndStrandFusion ORF
375 bp124 aa103477+No
ORF function : Transposase
Description : First part of the transposase

ORF sequence :

MAGPYSQDLRDRVAAAVSSGCSARAAAARFSISVSTAIRWAKRLGAEGHAQARPMGGDHRSRLSDHREAVLTLVAHQPDLTLEEIRAELNARHGVSVSLG
TVWRFLKAQNLTLKKNSARRRAAA

 

Blast result :
ORF 2
LengthBeginEndStrandFusion ORF
657 bp218 aa3891045+No
ORF function : Transposase
Description : Second part of the transposase

ORF sequence :

ASASARCGASSRPRTSPLKKTLHAAEQQRDDVVEARRAFIRRQPALDPERLVFLDETWASTNMTRRHGRCARGLRLLAPVPHGHWKTTTLIAGLRTTGIV
APYVLDGPINATVFRAYVEQVLAPTLQSGDILILDNLSSHKVAGVREAVEARGARVLYLPPYSPDLNPIEQAFAKLKALLRKAAERSRDGLWNAIGRIID
IYKPNECRNLFKNAGYPT

 

Blast result :
ORF 3
LengthBeginEndStrandFusion ORF
943 bp313 aa1031045+Yes
ORF function : Transposase
Chemistry : DDE

ORF sequence :

MAGPYSQDLRDRVAAAVSSGCSARAAAARFSISVSTAIRWAKRLGAEGHAQARPMGGDHRSRLSDHREAVLTLVAHQPDLTLEEIRAELNARHGVSVSLG
TVWRFLKAQNLTLKKTLHAAEQQRDDVVEARRAFIRRQPALDPERLVFLDETWASTNMTRRHGRCARGLRLLAPVPHGHWKTTTLIAGLRTTGIVAPYVL
DGPINATVFRAYVEQVLAPTLQSGDILILDNLSSHKVAGVREAVEARGARVLYLPPYSPDLNPIEQAFAKLKALLRKAAERSRDGLWNAIGRIIDIYKPN
ECRNLFKNAGYPT

 

Blast result :
Comments
ISAzs37 is 71% aa similar to ISMex41.
References
1] ISfinder annotation (2011)
2] Kaneko,T., Minamisawa,K., Isawa,T., Nakatsukasa,H., Mitsui,H., Kawaharada,Y., Nakamura,Y., Watanabe,A., Kawashima,K., Ono,A., Shimizu,Y., Takahashi,C., Minami,C., Fujishiro,T., Kohara,M., Katoh,M., Nakazaki,N., Nakayama,S., Yamada,M., Tabata,S. and Sato,S. (2010) DNA Res. 17 (1), 37-50.