ISfinder Mirror Site

ISAzs37

Family IS630
Group

MGE type ISRelated element(s) :
Isoform Synonym(s)

Accession number	Transposition	Origin	Host
NC_013860	ND	Azospirillum sp.	Azospirillum sp. B510 plasmid pAB510f

DNA section

IS Length : 1058 bp

Ends

IR Length : 18/24

IRL : GAGCGGTATTCGACCGGGTTGAATCGGTGTTTTTCTGTGGGATTCCCATT
IRR : GCGCGATTTTCGATCAGGTTGGATATCCCGCATTTTTGAAGAGGTTTCTG

Insertion site

Left flank	Direct repeat	Right flank	DR Length
CTGTGGAATCGGCGT	TA	GCGCGCCCTCAGAACC	2

DNA sequence

GAGCGGTATTCGACCGGGTTGAATCGGTGTTTTTCTGTGGGATTCCCATTTCTGGTAAAATCAGCGATTCTGAGCGGGTGATTAAGCGACCCGGAGCCGG
ACATGGCAGGCCCCTATTCTCAGGATCTTCGGGACCGCGTTGCCGCGGCGGTGTCGAGCGGCTGCTCGGCGCGCGCTGCGGCGGCGCGGTTCAGCATCAG
CGTCAGCACGGCGATCCGCTGGGCCAAACGCTTGGGCGCGGAAGGGCATGCCCAGGCGCGGCCAATGGGTGGCGACCACCGCTCGCGCCTGAGCGATCAC
CGTGAGGCGGTGCTGACGTTGGTCGCCCACCAGCCCGACCTGACGCTGGAGGAGATCCGCGCCGAACTGAACGCCCGCCATGGCGTGAGCGTCAGCCTCG
GCACGGTGTGGCGCTTCCTCAAGGCCCAGAACCTCACCCTTAAAAAAAACTCTGCACGCCGCCGAGCAGCAGCGTGATGACGTCGTCGAGGCCCGGCGCG
CCTTCATCCGCCGTCAGCCGGCCCTTGATCCCGAGCGCCTGGTGTTCCTTGATGAGACCTGGGCCAGCACCAACATGACCAGGCGCCACGGGCGGTGTGC
GCGCGGCTTGCGCCTCCTGGCTCCCGTCCCACACGGGCATTGGAAGACGACCACGCTGATCGCCGGCCTGCGCACCACCGGCATCGTCGCCCCCTATGTG
CTCGACGGGCCGATCAATGCCACCGTCTTCCGCGCCTACGTCGAGCAGGTTCTGGCCCCGACCCTGCAAAGTGGCGACATCCTCATCCTCGACAACCTGT
CCAGCCATAAGGTCGCCGGCGTCCGAGAAGCCGTCGAGGCGCGCGGCGCGCGGGTCCTCTACCTGCCGCCCTACAGCCCCGACCTCAACCCCATCGAGCA
AGCCTTCGCCAAGCTCAAGGCGCTCCTGCGAAAAGCGGCCGAGCGCTCCCGTGACGGACTCTGGAACGCCATCGGACGCATCATCGACATCTACAAGCCA
AACGAGTGCAGAAACCTCTTCAAAAATGCGGGATATCCAACCTGATCGAAAATCGCGC

Recoding section

Recoding by frameshift
Frame
Type
Experimentally demonstrated

Stimulators :

Shine-Dalgarno sequence :
Secondary structure :

Recoding motif :

Protein section

ORF number : 3

ORF 1

Length		Begin	End	Strand	Fusion ORF
375 bp	124 aa	103	477	+	No

ORF function : Transposase
Description : First part of the transposase

ORF sequence :

MAGPYSQDLRDRVAAAVSSGCSARAAAARFSISVSTAIRWAKRLGAEGHAQARPMGGDHRSRLSDHREAVLTLVAHQPDLTLEEIRAELNARHGVSVSLG
TVWRFLKAQNLTLKKNSARRRAAA

Blast result :

ORF 2

Length		Begin	End	Strand	Fusion ORF
657 bp	218 aa	389	1045	+	No

ORF function : Transposase
Description : Second part of the transposase

ORF sequence :

ASASARCGASSRPRTSPLKKTLHAAEQQRDDVVEARRAFIRRQPALDPERLVFLDETWASTNMTRRHGRCARGLRLLAPVPHGHWKTTTLIAGLRTTGIV
APYVLDGPINATVFRAYVEQVLAPTLQSGDILILDNLSSHKVAGVREAVEARGARVLYLPPYSPDLNPIEQAFAKLKALLRKAAERSRDGLWNAIGRIID
IYKPNECRNLFKNAGYPT

Blast result :

ORF 3

Length		Begin	End	Strand	Fusion ORF
943 bp	313 aa	103	1045	+	Yes

ORF function : Transposase
Chemistry : DDE

ORF sequence :

MAGPYSQDLRDRVAAAVSSGCSARAAAARFSISVSTAIRWAKRLGAEGHAQARPMGGDHRSRLSDHREAVLTLVAHQPDLTLEEIRAELNARHGVSVSLG
TVWRFLKAQNLTLKKTLHAAEQQRDDVVEARRAFIRRQPALDPERLVFLDETWASTNMTRRHGRCARGLRLLAPVPHGHWKTTTLIAGLRTTGIVAPYVL
DGPINATVFRAYVEQVLAPTLQSGDILILDNLSSHKVAGVREAVEARGARVLYLPPYSPDLNPIEQAFAKLKALLRKAAERSRDGLWNAIGRIIDIYKPN
ECRNLFKNAGYPT

Blast result :

Comments

ISAzs37 is 71% aa similar to ISMex41.

References

1] ISfinder annotation (2011)
2] Kaneko,T., Minamisawa,K., Isawa,T., Nakatsukasa,H., Mitsui,H., Kawaharada,Y., Nakamura,Y., Watanabe,A., Kawashima,K., Ono,A., Shimizu,Y., Takahashi,C., Minami,C., Fujishiro,T., Kohara,M., Katoh,M., Nakazaki,N., Nakayama,S., Yamada,M., Tabata,S. and Sato,S. (2010) DNA Res. 17 (1), 37-50.