ISfinder Mirror Site

ISAzs36

Family IS481
Group

MGE type ISRelated element(s) :
Isoform Synonym(s)

Accession number	Transposition	Origin	Host
NC_013860	ND	Azospirillum sp.	Azospirillum sp. B510 plasmid pAB510f

DNA section

IS Length : 2622 bp

Ends

IR Length : 17/29

IRL : TGTCTGAGGGCCGGCGGCAGGTTCCGTCAGAAGCACTGGTGAACCTGCGC
IRR : TGTTAGAGCGGCAGCCCAGCCGCCTGCATCTGTTGCGTCAGTTCCTGGGC

Insertion site

Left flank	Direct repeat	Right flank	DR Length
CAGCCGGCGA		GGACGCGGAA	0

DNA sequence

TGTCTGAGGGCCGGCGGCAGGTTCCGTCAGAAGCACTGGTGAACCTGCGCCGCCGGCTGGACGCCTTGCCGCCCCGTCATCCGGATCGCCTGGAAATCCT
GCGATCCGCCGCCGAGCTTTATGGGGTGTCCCGAGCCACGGTTTACCGCGCACTGCAACGGCAGTGGCGGCCGAAAGGGGTTCGTCGGGCCGATCGGGGG
AAGCCCCGCAAGCTCGATTTGGCCGAAATGGAGCGGTACTGCGAGATCATCGCCGCTCTGAAGCTACGGACCACCAACAAGAAGGGCCGGCATCTGTCCA
CCGCGCGAGCCATCGAACTGATGGAGGAGCATGGCGTGGAAACGCCTGACGGGTTGGTGAAGCTTCCGTCTGGATCGCTCACCCGGCCGACGGCGGACCG
ATATCTCAGGGCCTGGGGTTACGATCATGAGCGCCTGACCCGCGCGCCGGCGGCGGTCCGCTTCCAGGCACGACGGTCGAACGAGTTGTGGCAGTTCGAC
ATGAGCCCGTCGGACCTGAAGCATGTCAAGCAGCCGCTCTGGATCGAGCCCGGCCGCGGAGCGCCGACCCTGATGCTGTTCAGCATCGCTGACGACCGCA
GCGGCGTCGTCTACCAGGAATACCGGTGTGTCTACGGAGAGGACGCGGAATCGGCTCTGCGCTTCCTCTACAACGCCATGGCAGCCAAGCCCGAGGAGGA
CATGCCGTTCCAGGGTATCCCTGAGGCGATCTACATGGATAACGGTCCGGTCACCCGCTCACGCGTGTTTCAGAACGTCATGGCCTGCCTGGGCGTCAAG
GTCATGTCTCACCTGCCGGCGGGGAGCGACGGCCGGCGCACCACCGCCCGGTCCAAGGGCAAGGTCGAGCGGCCGTTCCGGACCGTCAAGGACGTCCATG
AGACGCTCTACCATTTCCACGAGCCGCAGGACGAGGCGGAAGCTAACCTGTGGCTGCGCCGCTTTCTTGTCCGCTACAACGCCCAGGATCACCGGTCCGA
GCCGCACAGCCGCCTCGAGGACTGGGTGCGGCACCTGCCGGAGAAGGGCTATCGCGAGATGTGTGCCTGGGACCGGTTCTGCGCTTTCGCGCGGGAGCCG
GAACGGCGCAAGGTCGGCATCGACACCCGTATCACCGTGGATGGCGTGGCCTACGAGGTCGATCCGGACCTGGCCGGCGAGGACGTGGTGCTCTGGTGGG
GGCTGTTCGACCAGGAACTCTACGTCGAGCATGGTGACCGGCGGTATGGTCCCTACGGCCCGGTCGGCGGTCCCATCCCGCTGCACAAGTACCGCAAGCA
TCAGAAGACCAAGTCCGAGGAGCGCGCCGACCGGGTGGCCGAACTGGCAGAGCGCATCGGCCTGCCGCGCGCGGCCCTGGACGGCGGCACCTCGCCCCTG
CCACCGCCCGTTACGGCACCGGCCGCCAAGGTGGCGTTCGCCGATCCGGACCCCTACCGCACCCTGACCTTTTCCGACATCATCGCAGCCAAGCGCGCGA
TCGCCGGCGAGATCGGCTTGGCGTTGGCGAAGCTGCCGCCCGAGGACCGGGCCTGGATCGACACGCTGGTGCGGGAGACCCTGGCCAAGGACGAGGTGCT
GACGAGGGTGCGGGCGTACTTCAGGCAGCGGACGGGAGGGGGCGGGGCATGCTGACGGAGGTCATGGAATATTTCAGGCTGGAGCGGGACCTGCTGATGG
CCGGGTATTATGAGACCGACCATCACCGCCAGCTCATCAAGGATGTGAAGGCGTCCATCCTGGCTGGCCGCCTGGTGGCGGTCGCCGGCGTCGTCGGCAG
TGGCAAAACAGTGCTGCTTCGCCGCATCCAGGATGAACTGACCCGCGAGGGGCGCGTCACGGTGTCGAAGAGCCTGTCGGTGGACAAGGACCGCACCACC
ATCCCGACCCTCATCACCGCGCTGTTCCACGACCTCTCGCCGGACAAGGACCCGAAGATCCCGACCCTGGGCGAGAAGCGGGAGCGCGAGTTGCAACAGC
TTGTGCGTCGCGGCCGCAAGCCGGTGGCCCTGTTCGTCGACGAGGCGCACGATCTGCATGGCAAGACGCTGATCGGCCTGAAACGGTTGATGGAGGTGGT
GGCCGATGGCGGCGGCATGCTCTCCGTGGTGCTGGTGGGCCATCCCAAACTCCGGAACGACCTCCGGCGCCCGACCATGGAAGAGATCGGCTACCGGTCG
GTGGTGTTCGAGTTCGACGGCATGGCGGGCAACCAGCTCGCCTACATCCGCTGGCTCCTCGCACGCTGTGCCGGCGAGGGCATCGACATCGGCGAGCTGA
TCGAGGCGGTGGCCGTGGAGTTGCTGGCCAGCCGCCTGAAGACCCCGCTTCAGATCGAGCAGCATCTGACCCTTGCGTTCGAGGAAGCATTCCGGATCGG
GGACCGACCGGTCACAGTCCAGGTGGTCGAGAGCATCCTGTCGCGTCAGCTCGACGATCTGGAACCGCGGCTGACCCGTCACGGCTACAGCGTGCGCTCC
CTGGCCGACCAGTTCAGCGTCAAACCGGCGGAGATCCGCCAGTTTCTCCGTGGTGAACTGGGAGGAGCCAGAGCCCAGGAACTGACGCAACAGATGCAGG
CGGCTGGGCTGCCGCTCTAACA

Protein section

ORF number : 2

ORF 1

Length		Begin	End	Strand	Fusion ORF
1635 bp	544 aa	21	1655	+	No

ORF function : Transposase
Chemistry : DDE

ORF sequence :

VPSEALVNLRRRLDALPPRHPDRLEILRSAAELYGVSRATVYRALQRQWRPKGVRRADRGKPRKLDLAEMERYCEIIAALKLRTTNKKGRHLSTARAIEL
MEEHGVETPDGLVKLPSGSLTRPTADRYLRAWGYDHERLTRAPAAVRFQARRSNELWQFDMSPSDLKHVKQPLWIEPGRGAPTLMLFSIADDRSGVVYQE
YRCVYGEDAESALRFLYNAMAAKPEEDMPFQGIPEAIYMDNGPVTRSRVFQNVMACLGVKVMSHLPAGSDGRRTTARSKGKVERPFRTVKDVHETLYHFH
EPQDEAEANLWLRRFLVRYNAQDHRSEPHSRLEDWVRHLPEKGYREMCAWDRFCAFAREPERRKVGIDTRITVDGVAYEVDPDLAGEDVVLWWGLFDQEL
YVEHGDRRYGPYGPVGGPIPLHKYRKHQKTKSEERADRVAELAERIGLPRAALDGGTSPLPPPVTAPAAKVAFADPDPYRTLTFSDIIAAKRAIAGEIGL
ALAKLPPEDRAWIDTLVRETLAKDEVLTRVRAYFRQRTGGGGAC

Blast result :

ORF 2

Length		Begin	End	Strand	Fusion ORF
974 bp	323 aa	1649	2622	+	No

ORF function : Passenger Gene
Annotation : AAA+ ATPaseDescription :

ORF sequence :

MLTEVMEYFRLERDLLMAGYYETDHHRQLIKDVKASILAGRLVAVAGVVGSGKTVLLRRIQDELTREGRVTVSKSLSVDKDRTTIPTLITALFHDLSPDK
DPKIPTLGEKRERELQQLVRRGRKPVALFVDEAHDLHGKTLIGLKRLMEVVADGGGMLSVVLVGHPKLRNDLRRPTMEEIGYRSVVFEFDGMAGNQLAYI
RWLLARCAGEGIDIGELIEAVAVELLASRLKTPLQIEQHLTLAFEEAFRIGDRPVTVQVVESILSRQLDDLEPRLTRHGYSVRSLADQFSVKPAEIRQFL
RGELGGARAQELTQQMQAAGLPL

Blast result :

Comments

ISAzs36 is 43% (ORFA : the transposase) aa similar to ISChy6. The second ORF is a passenger gene annotated as AAA+ ATPase.

References

1] ISfinder annotation (2011)
2] Kaneko,T., Minamisawa,K., Isawa,T., Nakatsukasa,H., Mitsui,H., Kawaharada,Y., Nakamura,Y., Watanabe,A., Kawashima,K., Ono,A., Shimizu,Y., Takahashi,C., Minami,C., Fujishiro,T., Kohara,M., Katoh,M., Nakazaki,N., Nakayama,S., Yamada,M., Tabata,S. and Sato,S. (2010) DNA Res. 17 (1), 37-50.