ISAzs35

  • Family IS110
  • Group IS1111
MGE type ISRelated element(s) :
Isoform Synonym(s)
Accession numberTranspositionOriginHost
NC_013855 ND Azospirillum sp.
Azospirillum sp. B510 plasmid pAB510f
Azospirillum sp. B510 plasmid pAB510a
Azospirillum sp. B510
DNA section
IS Length : 1524 bp

Ends


IR Length : 10/11

IRL : atcctATGAGGTGAAGAGCGACTGGAACCGGGTCAGAGTTCTGGTCCAGC
IRR : --tatATGAGGAGAATCAACGGAAATGTTCGTCAGCTTTTCATTTTATGC

Insertion site


Left flankDirect repeatRight flankDR Length
CGGGGTCGAGAGCCGATCTG0

DNA sequence

ATCCTATGAGGTGAAGAGCGACTGGAACCGGGTCAGAGTTCTGGTCCAGCACTGAACCCGGAGGCCGTCATGTCCAGCACATCCAATTCCACCGCCATTC
GTGTCGATCTCGGCGCGATCTTCGTTTCGCTTGAGCTGAGCCAGTCGAAATGGCTGGTCACATCACTGACGCCGGGTGGTGGCGAGAAGATGTCGAAACA
TCTGGTCAAGGCAGGGGATATTGGTGACCTGCTTTTGCGATTTGCGGAATTACGACGCAAGGCTCTGGTTCGGACTGGGCAAATCTTTCCAATTGTTGTG
ATCCAGGAAGCAGGGTTGGACGGCTTCTGGATTCATCGGGTCCTACTCCAGGAAGGGATCGAAAGTCACGTTGTCGACCCGGCATCGGTAGCGATTTCGC
GCCGCCGTCGTCGGGCAAAGACCGACAGGATTGACGGCGAGGCGCTCGTCCGAACGTTGCTGGCCTACAAGCGGGGCGAGCCTCGCGTTTGTGCAATGGT
AAGGCCGCCAACGCCTGAAGAGGAAGACCGGCGGCGGATCGCGCGGGAACGCAAGGTACTGGTCAATGAACGGGTAGCGCACGTCAATCGGATCAAGGGG
CTGCTCTACAGTCATGGCGTCGGAGCTTATGAACCGCTGCGGCGCGATCGCCGGGAGCGGCTTGATGAGCTCCGTACCGGAGATGGCCGCCCATTGCCAC
CCCACCTGCAGGCGCAGATTTCGCGGGAGCTTGACCGCCTTGAACTGGTGCTTGCACAGATAAAGGACATCGAGCGGGCGAGTACCGACATGCTTACCGC
AGAGGCGCTCGCATTTCCCGGCGCCGCTCTGTTGCTGGGCCTGAAAGGGATTGGAGCGGCGGCCGCGGCCGTGTTGTGGGGCGAAGGGCTTTGCCGGCAT
TTCGACAACCGCAGACAGGTCGCCGCCTACGCGGGCCTCGCGCCGACACCCTGGCAGAGTGGCCAGGTGGATCACGAGCAGGGAGTTTCAAAATCAGGTA
ATCCACGTCTGCGCACGACGTTGATCCAGATCGCCTGGCTGTGGAACCGCCACCAGCCGGATTCCGCACTGACGCTTTGGTCTAAGGATCAGGTGGCTAG
GAATGGGGGACGGCTCAAGAAGACGATGATCGTCGCCGTTGCCCGAAAACTGTTCGTCGCGCTTTGGAAATATACCCGTGCGGGCGTCGTCATTGAAGGT
GCCGTGCTGAAGGCCGCCTGACGTTCCGAGCGCTCATCAATTTCTCCGGGGAAAACCTTCCCGCTGGAGCCCGTACGGGCGAACCGAAGATCGGCATGGC
TGTCAAACAGCCGCGGTTTGAGAATGGTCTCGCCCAATTGAGCCTTGCCCGCCGCATGCGGGATGTTGGTGCAGTCGCCCCAGTCGACTGACCGTATATA
AGTTGGATCAGGCCCGGTTTCCGGGCCGCGTCATTGAAGCAGGCTCACGCCGTGGATACCAGATAGCGCTACAGGAGCATAAAATGAAAAGCTGACGAAC
ATTTCCGTTGATTCTCCTCATATA
Protein section
ORF number : 1

 

ORF 1
LengthBeginEndStrandFusion ORF
1152 bp383 aa701221+No
ORF function : Transposase
Chemistry : DEDD

ORF sequence :

MSSTSNSTAIRVDLGAIFVSLELSQSKWLVTSLTPGGGEKMSKHLVKAGDIGDLLLRFAELRRKALVRTGQIFPIVVIQEAGLDGFWIHRVLLQEGIESH
VVDPASVAISRRRRRAKTDRIDGEALVRTLLAYKRGEPRVCAMVRPPTPEEEDRRRIARERKVLVNERVAHVNRIKGLLYSHGVGAYEPLRRDRRERLDE
LRTGDGRPLPPHLQAQISRELDRLELVLAQIKDIERASTDMLTAEALAFPGAALLLGLKGIGAAAAAVLWGEGLCRHFDNRRQVAAYAGLAPTPWQSGQV
DHEQGVSKSGNPRLRTTLIQIAWLWNRHQPDSALTLWSKDQVARNGGRLKKTMIVAVARKLFVALWKYTRAGVVIEGAVLKAA

 

Blast result :
Comments
ISAzs35 is 82% aa similar to ISRel19.
References
1] ISfinder annotation (2011)
2] Kaneko,T., Minamisawa,K., Isawa,T., Nakatsukasa,H., Mitsui,H., Kawaharada,Y., Nakamura,Y., Watanabe,A., Kawashima,K., Ono,A., Shimizu,Y., Takahashi,C., Minami,C., Fujishiro,T., Kohara,M., Katoh,M., Nakazaki,N., Nakayama,S., Yamada,M., Tabata,S. and Sato,S. (2010) DNA Res. 17 (1), 37-50.