ISAzs34
- Family ISNCY
- Group IS892
Isoform Synonym(s)
| Accession number | Transposition | Origin | Host |
|---|---|---|---|
| NC_013856 | ND | Azospirillum sp. | Azospirillum sp. B510 plasmid pAB510b |
DNA section
IS Length : 1650 bp
Ends
IR Length : 33/40
IRL : CTAGGACGGCAAGCGTTGCTGGCTGTTGAACCTTCGAGAGGCGATTGCTC
IRR : CTAGGACGGCAAGCGTTGCTGGCATGCTCGGTCCTCGAGAACTGCGAGGT
Insertion site
| Left flank | Direct repeat | Right flank | DR Length |
|---|---|---|---|
| CCAGAACCTCACGCTCAAAAAAA | GTTTTTTAACGCTGTAGCCGCG | 0 |
DNA sequence
CTAGGACGGCAAGCGTTGCTGGCTGTTGAACCTTCGAGAGGCGATTGCTCTGGAGGGAGTTATGGCGCGGCGCGTCTGTCGGGTTCGCCAAGGTTCGGAA
CGTGTCTTGGTGCCGGAATGTCAGGATTGTCCGGAGTGCGGATTGCGGATGCGCATCCGCTATGAGAACCGCCGAACAGTGCTGACGCTGAGCGGGTTTG
AGCGGCTTCGGCTGAAGATCCAATGGTGCGAGAACCCGGCGTGCGCCCGCCACCACCGAGCCTATCGGCCGGAGGCGGAAGGGCAACTCACTCTGCCGCA
TCATGAGTTCGGGCTTGACGTGATTGCGCTGATCGGGTCCTTGCGCCATCGCGAGCATCGCAGCGTGCCGGAGATCCACGTCACCCTGCGCGAGCGCGGG
CTGCTGATCAGCGAGCGTAGCGTCACCAATCTGCTCGACCGTTACGATGAACTGGTTGCCACCGTCCTGGATGCGCCAAACCGGGCGGCGGTGGCTGCGC
AGGGCCGCGTCATCCTGGCGCTGGACGGCTTGCAGCCGGACGTCGGGCATGAGGTGCTTTGGGTGATCCGCGATTGCTTGTCGGGACGGGTCCTGCTGGC
CCGCCCGCTGCTCTCGGCGGCCCAGGCCGACCTCGCAGGGCTGTTGCGGGAAGTGATTGCGGCGCTGCCCGTGCCGGTGACCGGGGTGGTGAGCGACGGC
CAGCATTCCATCCGGCGTGCGGTGGCCGAGGCGTTGCCCGGCGTACCGCATCAATTGTGCCAGTTCCATTACCTGCGCGAGGCGGCGCGCCCCCTCTTCG
AGGCCGACCGCCACGCCAAGAAGGAACTCAAGAAAACCGTGCGCGGGGTGCGGCCGATCGAGCGCTGGAGCGAAGGGCACGAGGACGGCGGCGCGCCGGT
GGTTGCCGGCTACTGTGCCGCCGTGCGTTCGGCGCTTACCGACGACGGCCGCCCACCGCTGGCCGCCCCTGGCCTGAAACTGAAGGGCCGGTTGGAGGCG
ATTGCCGGCAGTCTCGACAGGGTCAAGAAAAAGGGGGGCTGAGCAAACCGCTGCGGCGGTTGCGCGACCTGTTGCGGCAGGGCTTGGAGCGCACCGCGGC
GCTGTGGCCGGACGTGCGCGAAACCCACCGCTGGCTGCACGCGGCAGCCCATATCCTGGGCAACGCCGCCGACCATCCCGCCACTGTGGTGCAGGCCCGC
TACGACCGCTTGATCGCCACATGGAGCACACGGCGCGGCCGGGCCGGCGGCCTGACCGCGGCCGTCGATCACTTCGTGAAGGTCACCGCCAGCTACCGGC
CCGGTCTCTTCCACTGCTACGCCGTGCCGGACCTGCCGCGCACCAACAACGATCTCGAACATCTGTTCGGCTGTCATCGCCATCATGAGCGACGGGCCAC
CGGGCGCAAAGTCGCATCGCCGTCCTTGGTGCTCCGCGGCTCCGTACGCATCGTGGCCGCCACCGCAACCCGCATCGCTCCCTTCACAGCCCGCGATCTC
GCAACCGACCAAGCACGATGGCTCAAACTCCGCAGCACACTGGAAACGCGACGCCAAGCCCGGGGCAGCCGAACACGCTTTCGACGCGATCAGGATACCT
ACCTCGCAGTTCTCGAGGACCGAGCATGCCAGCAACGCTTGCCGTCCTAG
CGTGTCTTGGTGCCGGAATGTCAGGATTGTCCGGAGTGCGGATTGCGGATGCGCATCCGCTATGAGAACCGCCGAACAGTGCTGACGCTGAGCGGGTTTG
AGCGGCTTCGGCTGAAGATCCAATGGTGCGAGAACCCGGCGTGCGCCCGCCACCACCGAGCCTATCGGCCGGAGGCGGAAGGGCAACTCACTCTGCCGCA
TCATGAGTTCGGGCTTGACGTGATTGCGCTGATCGGGTCCTTGCGCCATCGCGAGCATCGCAGCGTGCCGGAGATCCACGTCACCCTGCGCGAGCGCGGG
CTGCTGATCAGCGAGCGTAGCGTCACCAATCTGCTCGACCGTTACGATGAACTGGTTGCCACCGTCCTGGATGCGCCAAACCGGGCGGCGGTGGCTGCGC
AGGGCCGCGTCATCCTGGCGCTGGACGGCTTGCAGCCGGACGTCGGGCATGAGGTGCTTTGGGTGATCCGCGATTGCTTGTCGGGACGGGTCCTGCTGGC
CCGCCCGCTGCTCTCGGCGGCCCAGGCCGACCTCGCAGGGCTGTTGCGGGAAGTGATTGCGGCGCTGCCCGTGCCGGTGACCGGGGTGGTGAGCGACGGC
CAGCATTCCATCCGGCGTGCGGTGGCCGAGGCGTTGCCCGGCGTACCGCATCAATTGTGCCAGTTCCATTACCTGCGCGAGGCGGCGCGCCCCCTCTTCG
AGGCCGACCGCCACGCCAAGAAGGAACTCAAGAAAACCGTGCGCGGGGTGCGGCCGATCGAGCGCTGGAGCGAAGGGCACGAGGACGGCGGCGCGCCGGT
GGTTGCCGGCTACTGTGCCGCCGTGCGTTCGGCGCTTACCGACGACGGCCGCCCACCGCTGGCCGCCCCTGGCCTGAAACTGAAGGGCCGGTTGGAGGCG
ATTGCCGGCAGTCTCGACAGGGTCAAGAAAAAGGGGGGCTGAGCAAACCGCTGCGGCGGTTGCGCGACCTGTTGCGGCAGGGCTTGGAGCGCACCGCGGC
GCTGTGGCCGGACGTGCGCGAAACCCACCGCTGGCTGCACGCGGCAGCCCATATCCTGGGCAACGCCGCCGACCATCCCGCCACTGTGGTGCAGGCCCGC
TACGACCGCTTGATCGCCACATGGAGCACACGGCGCGGCCGGGCCGGCGGCCTGACCGCGGCCGTCGATCACTTCGTGAAGGTCACCGCCAGCTACCGGC
CCGGTCTCTTCCACTGCTACGCCGTGCCGGACCTGCCGCGCACCAACAACGATCTCGAACATCTGTTCGGCTGTCATCGCCATCATGAGCGACGGGCCAC
CGGGCGCAAAGTCGCATCGCCGTCCTTGGTGCTCCGCGGCTCCGTACGCATCGTGGCCGCCACCGCAACCCGCATCGCTCCCTTCACAGCCCGCGATCTC
GCAACCGACCAAGCACGATGGCTCAAACTCCGCAGCACACTGGAAACGCGACGCCAAGCCCGGGGCAGCCGAACACGCTTTCGACGCGATCAGGATACCT
ACCTCGCAGTTCTCGAGGACCGAGCATGCCAGCAACGCTTGCCGTCCTAG
Recoding section
- Recoding by frameshift
- Frame
- Type
- Experimentally demonstrated
Stimulators :
- Shine-Dalgarno sequence :
- Secondary structure :
Recoding motif :
Protein section
ORF number : 3
ORF 1
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 914 bp | 297 aa | 129 | 1042 | + | No |
Description : First part of the transposase
ORF sequence :
MRIRYENRRTVLTLSGFERLRLKIQWCENPACARHHRAYRPEAEGQLTLPHHEFGLDVIALIGSLRHREHRSVPEIHVTLRERGLLISERSVTNLLDRYD
ELVATVLDAPNRAAVAAQGRVILALDGLQPDVGHEVLWVIRDCLSGRVLLARPLLSAAQADLAGLLREVIAALPVPVTGVVSDGQHSIRRAVAEALPGVP
HQLCQFHYLREAARPLFEADRHAKKELKKTVRGVRPIERWSEGHEDGGAPVVAGYCAAVRSALTDDGRPPLAAPGLKLKGRLEAIAGSLDRVKKKGG
ELVATVLDAPNRAAVAAQGRVILALDGLQPDVGHEVLWVIRDCLSGRVLLARPLLSAAQADLAGLLREVIAALPVPVTGVVSDGQHSIRRAVAEALPGVP
HQLCQFHYLREAARPLFEADRHAKKELKKTVRGVRPIERWSEGHEDGGAPVVAGYCAAVRSALTDDGRPPLAAPGLKLKGRLEAIAGSLDRVKKKGG
Blast result :ORF 2
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 1098 bp | 365 aa | 553 | 1650 | + | No |
Description : Second part of the transposase
ORF sequence :
GALGDPRLLVGTGPAGPPAALGGPGRPRRAVAGSDCGAARAGDRGGERRPAFHPACGGRGVARRTASIVPVPLPARGGAPPLRGRPPRQEGTQENRARGA
ADRALERRARGRRRAGGCRLLCRRAFGAYRRRPPTAGRPWPETEGPVGGDCRQSRQGQEKGGLSKPLRRLRDLLRQGLERTAALWPDVRETHRWLHAAAH
ILGNAADHPATVVQARYDRLIATWSTRRGRAGGLTAAVDHFVKVTASYRPGLFHCYAVPDLPRTNNDLEHLFGCHRHHERRATGRKVASPSLVLRGSVRI
VAATATRIAPFTARDLATDQARWLKLRSTLETRRQARGSRTRFRRDQDTYLAVLEDRACQQRLPS
ADRALERRARGRRRAGGCRLLCRRAFGAYRRRPPTAGRPWPETEGPVGGDCRQSRQGQEKGGLSKPLRRLRDLLRQGLERTAALWPDVRETHRWLHAAAH
ILGNAADHPATVVQARYDRLIATWSTRRGRAGGLTAAVDHFVKVTASYRPGLFHCYAVPDLPRTNNDLEHLFGCHRHHERRATGRKVASPSLVLRGSVRI
VAATATRIAPFTARDLATDQARWLKLRSTLETRRQARGSRTRFRRDQDTYLAVLEDRACQQRLPS
Blast result :ORF 3
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 1522 bp | 500 aa | 129 | 1650 | + | Yes |
Chemistry : Unknow
ORF sequence :
MRIRYENRRTVLTLSGFERLRLKIQWCENPACARHHRAYRPEAEGQLTLPHHEFGLDVIALIGSLRHREHRSVPEIHVTLRERGLLISERSVTNLLDRYD
ELVATVLDAPNRAAVAAQGRVILALDGLQPDVGHEVLWVIRDCLSGRVLLARPLLSAAQADLAGLLREVIAALPVPVTGVVSDGQHSIRRAVAEALPGVP
HQLCQFHYLREAARPLFEADRHAKKELKKTVRGVRPIERWSEGHEDGGAPVVAGYCAAVRSALTDDGRPPLAAPGLKLKGRLEAIAGSLDRVKKKGGLSK
PLRRLRDLLRQGLERTAALWPDVRETHRWLHAAAHILGNAADHPATVVQARYDRLIATWSTRRGRAGGLTAAVDHFVKVTASYRPGLFHCYAVPDLPRTN
NDLEHLFGCHRHHERRATGRKVASPSLVLRGSVRIVAATATRIAPFTARDLATDQARWLKLRSTLETRRQARGSRTRFRRDQDTYLAVLEDRACQQRLPS
ELVATVLDAPNRAAVAAQGRVILALDGLQPDVGHEVLWVIRDCLSGRVLLARPLLSAAQADLAGLLREVIAALPVPVTGVVSDGQHSIRRAVAEALPGVP
HQLCQFHYLREAARPLFEADRHAKKELKKTVRGVRPIERWSEGHEDGGAPVVAGYCAAVRSALTDDGRPPLAAPGLKLKGRLEAIAGSLDRVKKKGGLSK
PLRRLRDLLRQGLERTAALWPDVRETHRWLHAAAHILGNAADHPATVVQARYDRLIATWSTRRGRAGGLTAAVDHFVKVTASYRPGLFHCYAVPDLPRTN
NDLEHLFGCHRHHERRATGRKVASPSLVLRGSVRIVAATATRIAPFTARDLATDQARWLKLRSTLETRRQARGSRTRFRRDQDTYLAVLEDRACQQRLPS
Blast result :
Comments
References
1] ISfinder annotation (2011)
2] Kaneko,T., Minamisawa,K., Isawa,T., Nakatsukasa,H., Mitsui,H., Kawaharada,Y., Nakamura,Y., Watanabe,A., Kawashima,K., Ono,A., Shimizu,Y., Takahashi,C., Minami,C., Fujishiro,T., Kohara,M., Katoh,M., Nakazaki,N., Nakayama,S., Yamada,M., Tabata,S. and Sato,S. (2010) DNA Res. 17 (1), 37-50.
2] Kaneko,T., Minamisawa,K., Isawa,T., Nakatsukasa,H., Mitsui,H., Kawaharada,Y., Nakamura,Y., Watanabe,A., Kawashima,K., Ono,A., Shimizu,Y., Takahashi,C., Minami,C., Fujishiro,T., Kohara,M., Katoh,M., Nakazaki,N., Nakayama,S., Yamada,M., Tabata,S. and Sato,S. (2010) DNA Res. 17 (1), 37-50.