ISAzs33
- Family IS3
- Group IS150
Isoform Synonym(s)
| Accession number | Transposition | Origin | Host |
|---|---|---|---|
| NC_013859 | ND | Azospirillum sp. | Azospirillum sp. B510 plasmid pAB510e |
DNA section
IS Length : 1265 bp
Ends
IR Length : 24/26
IRL : TGAGCTGGTCCCCGTTTCCCGGACAGTCGGATAAGCTTGGTTCGCCTATC
IRR : TGAGCTGGCCCCCATTTCCCGGACAGATTTTAGGCTAAAATAAGCTTGGT
Insertion site
| Left flank | Direct repeat | Right flank | DR Length |
|---|---|---|---|
| GTACCGGATAGCAAC | CTCCTGTTGC | 0 |
DNA sequence
TGAGCTGGTCCCCGTTTCCCGGACAGTCGGATAAGCTTGGTTCGCCTATCAGGAAGGACGGACCCGATGACGGGGAAACGGAAGCGGTATTCGGCGGAGT
TCAAGGCGAAGGTTGCGCTGGAGGCGATCCGGGGCGAGTTGACGGTGTCGCAACTGGTGGCCAAGCACGGCGTGCATCAGACGCTGATCAACGCTTGGAA
GAAGCAGGCGATGGATGGGATGGCAGGTGTGTTCTCCGGCAAGGCGGAAGCCGCCGAAACCAGCCATCAGGGCGAGGTGGAAAAGCTCCACACGATGATC
GGCCAGTTGGTGGTGGAGCGCGATTTTTTGCGCAAGGCCTCCGGTCGATGAGCGTCGGCCGGAGGCGGAAACTGATCGAGCCGGAGCATCCGCAGCTGTC
GATCACCCGACAGTGCGAGCTGGTTTCCATCAGCCGGTCCAGCTACTACGGGCCGGCCAAGGGCGAACCGGCCGTGAACCTGGACTTGATGCGGCTGATC
GACGGACAGTTCCTGGAAACGCCCTGGTACGGCAGCCGGCAGATGACCCGGCATCTGCGCCGTCATGGCCATGAGATCAACCGCAAGCGGGTTCGCCGCC
TGATGGCGCGGATGGGGTTGCAGGCGGTCTACCAGCGCCCGAAGACGACCGTGCCGCATCCCGAGCACAAGATCTGGCCGTACTTGCTGCGTGACATGAC
CATCGACCGACCCAACCAGGTCTGGTGCGCTGACATCACATACATTCCGATGCGTCGGGGCTTCCTGTATCTGGTAGCGGTGATGGACTGGGCAACGCGC
AAGGTCTTGAGCTGGCGGCTGTCGAACACCATGGACGTCGAGTTCTGCATCGAGGCCGTCGAGGAGGCGATGGCGCGGCACGGCCGGCCGGACATCTTCA
ACACCGATCAGGGCAGCCAGTTCACCAGCCCGCGCTTCACCGGCCTCTTGACCGCGGCCGGCATCCGGGTATCGAGGACGGGCGCGGCCGTTGGATGGAC
AACGTCTTCATCGAACGGCTGTGGCGATCGATGAAGTACGAGTGCGTCTATCTCCACGCCTTTGAGACCGGCAGCGAGGCCAGAGCCGGGATCGACCGCT
GGATCGATTACTACAACGCCGACCGCCCGCACTCGGCGCTCGGCGGCAGAACCCCGGCAGAGGCATACGAGGGCTCTCCGGACCAAAGCAGAATGGCGGC
ATAACACGAACCGGAACCAAGCTTATTTTAGCCTAAAATCTGTCCGGGAAATGGGGGCCAGCTCA
TCAAGGCGAAGGTTGCGCTGGAGGCGATCCGGGGCGAGTTGACGGTGTCGCAACTGGTGGCCAAGCACGGCGTGCATCAGACGCTGATCAACGCTTGGAA
GAAGCAGGCGATGGATGGGATGGCAGGTGTGTTCTCCGGCAAGGCGGAAGCCGCCGAAACCAGCCATCAGGGCGAGGTGGAAAAGCTCCACACGATGATC
GGCCAGTTGGTGGTGGAGCGCGATTTTTTGCGCAAGGCCTCCGGTCGATGAGCGTCGGCCGGAGGCGGAAACTGATCGAGCCGGAGCATCCGCAGCTGTC
GATCACCCGACAGTGCGAGCTGGTTTCCATCAGCCGGTCCAGCTACTACGGGCCGGCCAAGGGCGAACCGGCCGTGAACCTGGACTTGATGCGGCTGATC
GACGGACAGTTCCTGGAAACGCCCTGGTACGGCAGCCGGCAGATGACCCGGCATCTGCGCCGTCATGGCCATGAGATCAACCGCAAGCGGGTTCGCCGCC
TGATGGCGCGGATGGGGTTGCAGGCGGTCTACCAGCGCCCGAAGACGACCGTGCCGCATCCCGAGCACAAGATCTGGCCGTACTTGCTGCGTGACATGAC
CATCGACCGACCCAACCAGGTCTGGTGCGCTGACATCACATACATTCCGATGCGTCGGGGCTTCCTGTATCTGGTAGCGGTGATGGACTGGGCAACGCGC
AAGGTCTTGAGCTGGCGGCTGTCGAACACCATGGACGTCGAGTTCTGCATCGAGGCCGTCGAGGAGGCGATGGCGCGGCACGGCCGGCCGGACATCTTCA
ACACCGATCAGGGCAGCCAGTTCACCAGCCCGCGCTTCACCGGCCTCTTGACCGCGGCCGGCATCCGGGTATCGAGGACGGGCGCGGCCGTTGGATGGAC
AACGTCTTCATCGAACGGCTGTGGCGATCGATGAAGTACGAGTGCGTCTATCTCCACGCCTTTGAGACCGGCAGCGAGGCCAGAGCCGGGATCGACCGCT
GGATCGATTACTACAACGCCGACCGCCCGCACTCGGCGCTCGGCGGCAGAACCCCGGCAGAGGCATACGAGGGCTCTCCGGACCAAAGCAGAATGGCGGC
ATAACACGAACCGGAACCAAGCTTATTTTAGCCTAAAATCTGTCCGGGAAATGGGGGCCAGCTCA
Recoding section
- Recoding by frameshift
- Frame
- Type
- Experimentally demonstrated
Stimulators :
- Shine-Dalgarno sequence :
- Secondary structure :
Recoding motif :
Protein section
ORF number : 3
ORF 1
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 285 bp | 94 aa | 67 | 351 | + | No |
Description : First part of the transposase
ORF sequence :
MTGKRKRYSAEFKAKVALEAIRGELTVSQLVAKHGVHQTLINAWKKQAMDGMAGVFSGKAEAAETSHQGEVEKLHTMIGQLVVERDFLRKASGR
Blast result :ORF 2
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 962 bp | 320 aa | 243 | 1204 | + | No |
Description : Second part of the transposase
ORF sequence :
GGSRRNQPSGRGGKAPHDDRPVGGGARFFAQGLRSMSVGRRRKLIEPEHPQLSITRQCELVSISRSSYYGPAKGEPAVNLDLMRLIDGQFLETPWYGSRQ
MTRHLRRHGHEINRKRVRRLMARMGLQAVYQRPKTTVPHPEHKIWPYLLRDMTIDRPNQVWCADITYIPMRRGFLYLVAVMDWATRKVLSWRLSNTMDVE
FCIEAVEEAMARHGRPDIFNTDQGSQFTSPRFTGLLTAAGIRVSRDGRGRWMDNVFIERLWRSMKYECVYLHAFETGSEARAGIDRWIDYYNADRPHSAL
GGRTPAEAYEGSPDQSRMAA
MTRHLRRHGHEINRKRVRRLMARMGLQAVYQRPKTTVPHPEHKIWPYLLRDMTIDRPNQVWCADITYIPMRRGFLYLVAVMDWATRKVLSWRLSNTMDVE
FCIEAVEEAMARHGRPDIFNTDQGSQFTSPRFTGLLTAAGIRVSRDGRGRWMDNVFIERLWRSMKYECVYLHAFETGSEARAGIDRWIDYYNADRPHSAL
GGRTPAEAYEGSPDQSRMAA
Blast result :ORF 3
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 1138 bp | 379 aa | 67 | 1204 | + | Yes |
Chemistry : DDE
ORF sequence :
MTGKRKRYSAEFKAKVALEAIRGELTVSQLVAKHGVHQTLINAWKKQAMDGMAGVFSGKAEAAETSHQGEVEKLHTMIGQLVVERDFFAQGLRSMSVGRR
RKLIEPEHPQLSITRQCELVSISRSSYYGPAKGEPAVNLDLMRLIDGQFLETPWYGSRQMTRHLRRHGHEINRKRVRRLMARMGLQAVYQRPKTTVPHPE
HKIWPYLLRDMTIDRPNQVWCADITYIPMRRGFLYLVAVMDWATRKVLSWRLSNTMDVEFCIEAVEEAMARHGRPDIFNTDQGSQFTSPRFTGLLTAAGI
RVSRDGRGRWMDNVFIERLWRSMKYECVYLHAFETGSEARAGIDRWIDYYNADRPHSALGGRTPAEAYEGSPDQSRMAA
RKLIEPEHPQLSITRQCELVSISRSSYYGPAKGEPAVNLDLMRLIDGQFLETPWYGSRQMTRHLRRHGHEINRKRVRRLMARMGLQAVYQRPKTTVPHPE
HKIWPYLLRDMTIDRPNQVWCADITYIPMRRGFLYLVAVMDWATRKVLSWRLSNTMDVEFCIEAVEEAMARHGRPDIFNTDQGSQFTSPRFTGLLTAAGI
RVSRDGRGRWMDNVFIERLWRSMKYECVYLHAFETGSEARAGIDRWIDYYNADRPHSALGGRTPAEAYEGSPDQSRMAA
Blast result :
Comments
ISAzs33 is 95% aa similar to ISAli5.
References
1] ISfinder annotation (2010).
2] Kaneko,T., Minamisawa,K., Isawa,T., Nakatsukasa,H., Mitsui,H., Kawaharada,Y., Nakamura,Y., Watanabe,A., Kawashima,K., Ono,A., Shimizu,Y., Takahashi,C., Minami,C., Fujishiro,T., Kohara,M., Katoh,M., Nakazaki,N., Nakayama,S., Yamada,M., Tabata,S. and Sato,S. (2010) DNA Res. 17 (1), 37-50.
2] Kaneko,T., Minamisawa,K., Isawa,T., Nakatsukasa,H., Mitsui,H., Kawaharada,Y., Nakamura,Y., Watanabe,A., Kawashima,K., Ono,A., Shimizu,Y., Takahashi,C., Minami,C., Fujishiro,T., Kohara,M., Katoh,M., Nakazaki,N., Nakayama,S., Yamada,M., Tabata,S. and Sato,S. (2010) DNA Res. 17 (1), 37-50.