ISfinder Mirror Site

ISAzs31

Family IS630
Group

MGE type ISRelated element(s) :
Isoform Synonym(s)

Accession number	Transposition	Origin	Host
NC_013856	ND	Azospirillum sp.	Azospirillum sp. B510 plasmid pAB510b

DNA section

IS Length : 1160 bp

Ends

IR Length : 17/26

IRL : GTGTTCTGACCGAAACAGACGATTCCCCTTTGTCGCGCGCTGTGCGAGTG
IRR : GTGTTCCGACTCTAACGTTTGGTTCCGATGGTTTGGCGTAATGCGGTTTT

Insertion site

Left flank	Direct repeat	Right flank	DR Length
AACTGTAGTAC	TA	GAGCACGCTCAA	2

DNA sequence

GTGTTCTGACCGAAACAGACGATTCCCCTTTGTCGCGCGCTGTGCGAGTGTTGAGTCATGAGAACGGGGATTTTGTTCCAGCTCTCGCCTGAGGATCGGG
CTCGGTTGGACAGGCTTGTGGCAGACCGGAACACCCGTCAAAAGCACGTTCGCCGTGCGCAGATCGTGCTGCTGACGGCTGAGGGTGTCGGGACGATGGA
AATCATGGAGCGAACCGGGCAGTCGAAACCGACGGTCTGGCGCTGGCAGGCGCGGTTTGCCGAAGCGGGTGTGGACGGACTGCTGCGCGACAAGACGCGG
CCACCGGGCAAAAAGCCGCTTTCGGAAGCAACGGTGCGTCAAGTGGTGACCAAGACGACCACGGAACGGCCTCCCGATGCGACGCATTGGAGTGCCCGGG
CCATGGCCCGGGCCGTTGGGATCGCGGTCAGCAGCGTCCAGAAAATCTGGCAGGCCCATGGGCTGAAACCGCATCAGGTCCGGACCTTCAAACTATCCAA
CGATCCCGATTTCGAAGCCAAACTGGTCGATGTCGTCGGTCTCTACCTTAATCCGCCGGATCGGGCACTGGTGCTGTCGGTGGACGAGAAGAGCCAAATC
CAGGCGCTGGACCGCACCCAGCCGGGATTGCCGTTGACGAAAGGGCGGGCAGGCACCCTGACCCATGACTACAAGCGCCATGGCACTACGACCCTGTTCG
CCGCTCTGAACCTGCTGGACGGTCGGGTGATCGGGCGCTGCATGGACAAGCACCGGCATCAGGAGTTCATCAGATTCCTCGACACCATCGACGCCGACAC
GCCAGCCGACATCGACCTTCACCTGATCGTGGACAACTACGGCACCCACAAGCACGCCGAAGTCGCCGCCTGGCTTGAACGCCATCCCCGGTTCCACCTC
CATTTCACGCCGACCGCGTCGTCCTGGCTGAACATGGTCGAGTGGTTCTTCGCCGAAATCACCCGCAAGCGCATTCGCCGTGGCGTCTTCAGGAGCGTCA
CCGATCTGGAGGTCGCGATTTACCGGTATCTTGCCGAACACAACGAGCATCCAAAGCCGTTCGTTTGGACGGCGACCACCAAGGAAATACTCGCCAAGGT
CGAAAAAGCCAAAACCGCATTACGCCAAACCATCGGAACCAAACGTTAGAGTCGGAACAC

Protein section

ORF number : 1

ORF 1

Length		Begin	End	Strand	Fusion ORF
1092 bp	363 aa	58	1149	+	No

ORF function : Transposase
Chemistry : DDE

ORF sequence :

MRTGILFQLSPEDRARLDRLVADRNTRQKHVRRAQIVLLTAEGVGTMEIMERTGQSKPTVWRWQARFAEAGVDGLLRDKTRPPGKKPLSEATVRQVVTKT
TTERPPDATHWSARAMARAVGIAVSSVQKIWQAHGLKPHQVRTFKLSNDPDFEAKLVDVVGLYLNPPDRALVLSVDEKSQIQALDRTQPGLPLTKGRAGT
LTHDYKRHGTTTLFAALNLLDGRVIGRCMDKHRHQEFIRFLDTIDADTPADIDLHLIVDNYGTHKHAEVAAWLERHPRFHLHFTPTASSWLNMVEWFFAE
ITRKRIRRGVFRSVTDLEVAIYRYLAEHNEHPKPFVWTATTKEILAKVEKAKTALRQTIGTKR

Blast result :

Comments

ISAzs31 is 76% aa similar to IS870.

References

1] ISfinder annotation (2010).
2] Kaneko,T., Minamisawa,K., Isawa,T., Nakatsukasa,H., Mitsui,H., Kawaharada,Y., Nakamura,Y., Watanabe,A., Kawashima,K., Ono,A., Shimizu,Y., Takahashi,C., Minami,C., Fujishiro,T., Kohara,M., Katoh,M., Nakazaki,N., Nakayama,S., Yamada,M., Tabata,S. and Sato,S. (2010) DNA Res. 17 (1), 37-50.