ISAzs30
- Family IS21
- Group
Isoform Synonym(s)
| Accession number | Transposition | Origin | Host |
|---|---|---|---|
| NC_013856 | ND | Azospirillum sp. | Azospirillum sp. B510 plasmid pAB510b |
DNA section
IS Length : 2365 bp
Ends
IR Length : 38/52
IRL : TGTCAGTGCCGATTGGATACTCCCCAGAAAGGGCGGCTGAAAATTCCCCA
IRR : TGTAAAGGCCGAAAGAAGTTTCCCCAGAACGGTCGGTCGAAAAATCCCCA
Insertion site
| Left flank | Direct repeat | Right flank | DR Length |
|---|---|---|---|
| CTAATTACCCACATCGTCAT | TGAGG | ATAGTGTCCGCGAACGTGG | 5 |
DNA sequence
TGTCAGTGCCGATTGGATACTCCCCAGAAAGGGCGGCTGAAAATTCCCCAGGCTGACGCAACGGCAGGGCTCCGGGGCGTACGCCCCGGAGCCCTGCGGC
CGCTCATCGCCCATGATCACCCTCCGAGGCGGAGGGAGGGCACAGGTGGTCAGACTTGGGGAACTCGTCATGATTCTCGATCTCGCCCGCGAGGGCCTGT
CGGTGTCGGCGATCTCACGCCGCACCGGCCTGGATCGCAAGACCATCCGCAAATACATCGCCCGCGGACTGGAGGCGCCGGCCTACACCCCTCGGCCGGC
TCGGCCGACGCTGCTCACGCCTTTCGAGCCCTATCTGCGCGAACGCCTACAGGGCTTTCCTGATCTCAGCGCCCGCCGCCTGCTGCGGGAACTCAAGGAG
CGCGGTTACAGCGGCGGCTATACCATCCTGAAAGCCGTGGTCCGCGCCATCCGTCCTACCCCGTCACCGTCCTTCGAGCGTCGCTTCGAGACGCCGCCCG
GCAAGCAGGCCCAGGTCGATTTCGCCTTCTTCAAGACGACCTTCACCGACGAGCCCGAGGTGGAGCGGATCGTCTGGCTGTTCTCCATGGTGCTCGGCCA
CAGCCGCATGATGTGGGCCCGCTTCGTCGCCCGCCAGGACCTGCAAACCGTGCTCCGCTGCCACATCGCCGCCTTCGACAGCTTCGGCGGCGTGCCGGAA
CAGATCCTCTACGATCGCATGAAAACCGCCGTGCTGGGCGAGATCGAAGACCCCGATCAGCCGACCCAGGGGATCGCCTACAATCCCAAGCTCCTCTCCC
TGGCCAGCCACTACGGTTTCCTGCCGAAGGCCTGCAAGCCGTACCGCGCCAAAACCAAGGGCAAAGTCGAACGGCCGTTCCGCTATGTGCGTGAGGACTT
CTTCCTGGCCCGCAGCTTCCGCAATCTCGAGGACCTGAACATCCAGTTCACCCAGTGGCTGGATCAACTCGCCAACCGCCGTCGGCACGCCACCACCCAG
CGCATCGTCGCCGAGCATTTCGCCGAGGAGCGCCCCCACCTCAAGCCGCTGCCGGCCGGCCCCTTCAACAGCGTGCTGGCCCTGGAGCGCCGTGTCACCC
GCGACGGCATGGTGTCGGTCGGCGGCAACCTCTACTCCGTGCCGGATTGCACCCGCCGGCGGACGGTGGAGGTGCAGGTCACCGCCGGCACCGTCACCAT
CCTGGAGAACGGCATAGCCGTCGCATCCCACCCGGCGCTGGAAGGACGAGGCCAGCGGCGCATTGCCACCGGCCATCGGACCCAGCCTCCACCGCCCAAC
AGCCAGACGCCCAGGGAGGTTGCCCCGCCCCCGGCACCTCCGCTGCAGGGCGGCACCGTCACGCCCCGCTCGCTGGCCATCTACGACACCATCGGCCGCC
ATTTGGCCAACCAGCCGGGGAGCCCGTGATGAGCACCACGTCGCTGGAAGCCGGCCCCGCCACCCTCGACCGCATTCGCCAATATCTGGTTGGGCTGAAG
ATGCCGCGCGCCTTGGAAGCGCTGGAGCACATCCTGCGCCGGCTGGAACGCGGCGAACTCTCCGCTCTGGAGGCCATCGACGCCCTGCTGGGCGAGGAAC
TGACGTTGCGCGAGGGACGCCGCATCAAGGCGGCCCTGAAGATGGGCCGGCTTCTCTCCATCAAGACGCTCACCGGCTTCGACTTCTCCTTCCAACCCTC
GCTCGATCGCGATCGGATCATGACCTTGGCGCAACTCGACTTCGTCGACCGGCACGAGGTGGTCCATTTCCTCGGCCAACCCGGCTGCGGCAAGACGCAC
CTGGCGCTCGCTCTGGGGGTGGAGGCCGTCAAGGCCGGCCGCTCGGTCTACTTCGCCACCCTGGCCGACATCGTAAGCTCGCTGGCCAAGGCCGAACGTG
AAGGGACTTTGCGGGAACGTCTGCGCTTCCTGTGCCGGCCACAGCTGCTGATCGTCGACGAGATCGGCTACCTGCCGGTGATCCCCGGCGGCGGCAACCT
GTTCTTCCAGCTCGTCAACGCCCGCTATGAACGCGGCGCCATGATCCTGACCTCCAACCGCGGCTTTTCCGAATGGGGCGATGTCTTCGGCGGCACCGTC
GTCGCTTCCGCCCTGCTGGACCGCCTGCTGCACCATGCTGTGGTGGTGCAGATCGAAGGCGCCAGCTACCGCCTGCGCCGCCATGCCGAACTGATCCCGG
ACAAAAACCGCAGCAGGCCGATCACCGCCCCATCACCGGCGCTCCGGCGCCGAGGCCGTCCACCAAAAGCAAGGAGCGCCGACACCGAAGCCAATCCATA
GCCCTTCAAACCATCTGGGGATTTTTCGACCGACCGTTCTGGGGAAACTTCTTTCGGCCTTTACA
CGCTCATCGCCCATGATCACCCTCCGAGGCGGAGGGAGGGCACAGGTGGTCAGACTTGGGGAACTCGTCATGATTCTCGATCTCGCCCGCGAGGGCCTGT
CGGTGTCGGCGATCTCACGCCGCACCGGCCTGGATCGCAAGACCATCCGCAAATACATCGCCCGCGGACTGGAGGCGCCGGCCTACACCCCTCGGCCGGC
TCGGCCGACGCTGCTCACGCCTTTCGAGCCCTATCTGCGCGAACGCCTACAGGGCTTTCCTGATCTCAGCGCCCGCCGCCTGCTGCGGGAACTCAAGGAG
CGCGGTTACAGCGGCGGCTATACCATCCTGAAAGCCGTGGTCCGCGCCATCCGTCCTACCCCGTCACCGTCCTTCGAGCGTCGCTTCGAGACGCCGCCCG
GCAAGCAGGCCCAGGTCGATTTCGCCTTCTTCAAGACGACCTTCACCGACGAGCCCGAGGTGGAGCGGATCGTCTGGCTGTTCTCCATGGTGCTCGGCCA
CAGCCGCATGATGTGGGCCCGCTTCGTCGCCCGCCAGGACCTGCAAACCGTGCTCCGCTGCCACATCGCCGCCTTCGACAGCTTCGGCGGCGTGCCGGAA
CAGATCCTCTACGATCGCATGAAAACCGCCGTGCTGGGCGAGATCGAAGACCCCGATCAGCCGACCCAGGGGATCGCCTACAATCCCAAGCTCCTCTCCC
TGGCCAGCCACTACGGTTTCCTGCCGAAGGCCTGCAAGCCGTACCGCGCCAAAACCAAGGGCAAAGTCGAACGGCCGTTCCGCTATGTGCGTGAGGACTT
CTTCCTGGCCCGCAGCTTCCGCAATCTCGAGGACCTGAACATCCAGTTCACCCAGTGGCTGGATCAACTCGCCAACCGCCGTCGGCACGCCACCACCCAG
CGCATCGTCGCCGAGCATTTCGCCGAGGAGCGCCCCCACCTCAAGCCGCTGCCGGCCGGCCCCTTCAACAGCGTGCTGGCCCTGGAGCGCCGTGTCACCC
GCGACGGCATGGTGTCGGTCGGCGGCAACCTCTACTCCGTGCCGGATTGCACCCGCCGGCGGACGGTGGAGGTGCAGGTCACCGCCGGCACCGTCACCAT
CCTGGAGAACGGCATAGCCGTCGCATCCCACCCGGCGCTGGAAGGACGAGGCCAGCGGCGCATTGCCACCGGCCATCGGACCCAGCCTCCACCGCCCAAC
AGCCAGACGCCCAGGGAGGTTGCCCCGCCCCCGGCACCTCCGCTGCAGGGCGGCACCGTCACGCCCCGCTCGCTGGCCATCTACGACACCATCGGCCGCC
ATTTGGCCAACCAGCCGGGGAGCCCGTGATGAGCACCACGTCGCTGGAAGCCGGCCCCGCCACCCTCGACCGCATTCGCCAATATCTGGTTGGGCTGAAG
ATGCCGCGCGCCTTGGAAGCGCTGGAGCACATCCTGCGCCGGCTGGAACGCGGCGAACTCTCCGCTCTGGAGGCCATCGACGCCCTGCTGGGCGAGGAAC
TGACGTTGCGCGAGGGACGCCGCATCAAGGCGGCCCTGAAGATGGGCCGGCTTCTCTCCATCAAGACGCTCACCGGCTTCGACTTCTCCTTCCAACCCTC
GCTCGATCGCGATCGGATCATGACCTTGGCGCAACTCGACTTCGTCGACCGGCACGAGGTGGTCCATTTCCTCGGCCAACCCGGCTGCGGCAAGACGCAC
CTGGCGCTCGCTCTGGGGGTGGAGGCCGTCAAGGCCGGCCGCTCGGTCTACTTCGCCACCCTGGCCGACATCGTAAGCTCGCTGGCCAAGGCCGAACGTG
AAGGGACTTTGCGGGAACGTCTGCGCTTCCTGTGCCGGCCACAGCTGCTGATCGTCGACGAGATCGGCTACCTGCCGGTGATCCCCGGCGGCGGCAACCT
GTTCTTCCAGCTCGTCAACGCCCGCTATGAACGCGGCGCCATGATCCTGACCTCCAACCGCGGCTTTTCCGAATGGGGCGATGTCTTCGGCGGCACCGTC
GTCGCTTCCGCCCTGCTGGACCGCCTGCTGCACCATGCTGTGGTGGTGCAGATCGAAGGCGCCAGCTACCGCCTGCGCCGCCATGCCGAACTGATCCCGG
ACAAAAACCGCAGCAGGCCGATCACCGCCCCATCACCGGCGCTCCGGCGCCGAGGCCGTCCACCAAAAGCAAGGAGCGCCGACACCGAAGCCAATCCATA
GCCCTTCAAACCATCTGGGGATTTTTCGACCGACCGTTCTGGGGAAACTTCTTTCGGCCTTTACA
Protein section
ORF number : 2
ORF 1
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 1284 bp | 427 aa | 146 | 1429 | + | No |
Chemistry : DDE
ORF sequence :
MVRLGELVMILDLAREGLSVSAISRRTGLDRKTIRKYIARGLEAPAYTPRPARPTLLTPFEPYLRERLQGFPDLSARRLLRELKERGYSGGYTILKAVVR
AIRPTPSPSFERRFETPPGKQAQVDFAFFKTTFTDEPEVERIVWLFSMVLGHSRMMWARFVARQDLQTVLRCHIAAFDSFGGVPEQILYDRMKTAVLGEI
EDPDQPTQGIAYNPKLLSLASHYGFLPKACKPYRAKTKGKVERPFRYVREDFFLARSFRNLEDLNIQFTQWLDQLANRRRHATTQRIVAEHFAEERPHLK
PLPAGPFNSVLALERRVTRDGMVSVGGNLYSVPDCTRRRTVEVQVTAGTVTILENGIAVASHPALEGRGQRRIATGHRTQPPPPNSQTPREVAPPPAPPL
QGGTVTPRSLAIYDTIGRHLANQPGSP
AIRPTPSPSFERRFETPPGKQAQVDFAFFKTTFTDEPEVERIVWLFSMVLGHSRMMWARFVARQDLQTVLRCHIAAFDSFGGVPEQILYDRMKTAVLGEI
EDPDQPTQGIAYNPKLLSLASHYGFLPKACKPYRAKTKGKVERPFRYVREDFFLARSFRNLEDLNIQFTQWLDQLANRRRHATTQRIVAEHFAEERPHLK
PLPAGPFNSVLALERRVTRDGMVSVGGNLYSVPDCTRRRTVEVQVTAGTVTILENGIAVASHPALEGRGQRRIATGHRTQPPPPNSQTPREVAPPPAPPL
QGGTVTPRSLAIYDTIGRHLANQPGSP
Blast result :ORF 2
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 873 bp | 290 aa | 1429 | 2301 | + | No |
AG : IS21 helper
ORF sequence :
MSTTSLEAGPATLDRIRQYLVGLKMPRALEALEHILRRLERGELSALEAIDALLGEELTLREGRRIKAALKMGRLLSIKTLTGFDFSFQPSLDRDRIMTL
AQLDFVDRHEVVHFLGQPGCGKTHLALALGVEAVKAGRSVYFATLADIVSSLAKAEREGTLRERLRFLCRPQLLIVDEIGYLPVIPGGGNLFFQLVNARY
ERGAMILTSNRGFSEWGDVFGGTVVASALLDRLLHHAVVVQIEGASYRLRRHAELIPDKNRSRPITAPSPALRRRGRPPKARSADTEANP
AQLDFVDRHEVVHFLGQPGCGKTHLALALGVEAVKAGRSVYFATLADIVSSLAKAEREGTLRERLRFLCRPQLLIVDEIGYLPVIPGGGNLFFQLVNARY
ERGAMILTSNRGFSEWGDVFGGTVVASALLDRLLHHAVVVQIEGASYRLRRHAELIPDKNRSRPITAPSPALRRRGRPPKARSADTEANP
Blast result :
Comments
ISAzs30 is 71% (ORFA : the transposase)and 88% (ORFB : the helper of transposition) aa similar to ISMtsp8.
References
1] ISfinder annotation (2010).
2] Kaneko,T., Minamisawa,K., Isawa,T., Nakatsukasa,H., Mitsui,H., Kawaharada,Y., Nakamura,Y., Watanabe,A., Kawashima,K., Ono,A., Shimizu,Y., Takahashi,C., Minami,C., Fujishiro,T., Kohara,M., Katoh,M., Nakazaki,N., Nakayama,S., Yamada,M., Tabata,S. and Sato,S. (2010) DNA Res. 17 (1), 37-50.
2] Kaneko,T., Minamisawa,K., Isawa,T., Nakatsukasa,H., Mitsui,H., Kawaharada,Y., Nakamura,Y., Watanabe,A., Kawashima,K., Ono,A., Shimizu,Y., Takahashi,C., Minami,C., Fujishiro,T., Kohara,M., Katoh,M., Nakazaki,N., Nakayama,S., Yamada,M., Tabata,S. and Sato,S. (2010) DNA Res. 17 (1), 37-50.