ISfinder Mirror Site

ISAzs29

Family Tn3
Group

MGE type ISRelated element(s) :
Isoform Synonym(s) TnAzs29

Accession number	Transposition	Origin	Host
NC_013855	ND	Azospirillum sp.	Azospirillum sp. B510 plasmid pAB510a

DNA section

IS Length : 3897 bp

Ends

IR Length : 43/46

IRL : GGGGTGACGCCATAGGACGGGCAAGAATATACGCTAAGCGGACAAATCAG
IRR : GGGGTGACGCCATAGAATGGGCGAGAATATACGCTAAGCGGACAAAGCCG

Insertion site

Left flank	Direct repeat	Right flank	DR Length
GGTCGCCGCGATCATGGCACGAAGC		AGGTAAGGCCAGTCGCCCTCCAGCGGAC	0

DNA sequence

GGGGTGACGCCATAGGACGGGCAAGAATATACGCTAAGCGGACAAATCAGGAACGCTCGCGGCTGATGGCACCACCGCTTGGACTGACGTGTGGACGCCA
ACAGCTATTCCGCGGATGGAAAATATGCGTGTGGCCGTTGCATGGATCCGGCATGAAACCAAGCGCTTCCCAGAATGCCGAGGCATCAGCGGTGCCGGCG
TTGACCATCACTTGGCATTCCAAGGATTGGGATCTCTCAAGAAGAGAGAGGGCCAACTTGCGGCCGATGCCGCGGTGCCGAAGCGACGGTCGCACATAGA
AGCGCCGCATACGTAAGGCGCCCATTACAGCAGAATCGATGGTCATCCCTCCGATACCAGCCAGTTCGCTTCCAACATAGGCGGCGAGCAATACCTCTCC
TTCACGGGCGAAGCGCTGCCTGTGCGATATCCACTCGGTCACGAGCCGCTCCAGGAATCGGTGGCCCTCGCAAGCTGCCTCGGCTCGTAGAACGTCGATA
CCCAGGGGTAAGCCATAAAGGACAGGGGCGAGATGAACTGCCGAATTCATTCGTTGGCCTCAGCCACATCACGGTGCCGTTTCACGATATCCGTCACGGT
ATTCTTGCTGAGCCCCAGTTCCCGGCCGATCAGACGATAACTGCGACCCTTGAAAACGCCCACCACTTGGTAGTCGGCGCGGGCGGCGAAGGCGAGGAGG
TCGCGCTCTTGTCGCGCGCAGGACTGGTCGGCGGTGGAGACCCGGCAGTAGAGGGCGGCACGCTGTCCCATTTGAACCCTCCCGGATTCATGCCCGGCAA
ACCCGCGTCCCGGCTAGGGTCGCCGCCAGCCAAAACAGACAATACTCCAACTGGGACAGCTCCTCCATGCCGCGCCGCCGCGTCCTCACCGACGCCCAGA
TCACCGCCCTGTTCGCCCTGCCCACCGCCGAGGCCGACCTCATCCGCCACTACACCCTCGGCCCCGACGATTTGGCGGTCATCGCCCGGCGCCGCCGGCC
GCACAACCGTCTCGGCTTCGCCCTACAGCTCTGCGCCCTGCGCTATCCCGGCCGGCTGATCCGGCCCGGCGAGCTGGTCCCGCCGACGATTGTCGCATTC
CTGGCGGAGCAGCTGGACATCGACCCGGACACCCTCGCCGACTATGCGGCCCGTGCACCGACCCGCTACGATCAGCTGGACGCGCTGCACGAGGCCTTCG
GATTCAGCACCTTCACCCAGCCCGATCATCGCGAACTCTCGGCGTGGCTGGTGCCGATCGCCCTGGCGACGGTCAGCGCGATCACGGTCGCCGAGGCGCT
GATGGCCGAGTTGCGGCGGCGACGCATTGCCGCCCCGGGAACCACCACGATCGAGCGCATGGTGGCCACTGCCCTCCTGCTGGCGGAACGCCACGCCGCC
GCCAAGCTGAACTCCACCCTGTCTCCGGAACGCTGCACGGCATTGGACGCGCTGCTGGACAACCATCCGGGAACCGCGGTCAGCTCCCTGGCCTGGACCC
GGCAGCCGCCCGGCGCGCCCGGTCATCGGTCCATGGCGCGCCTGGTCGACCAACTTCGGCATCTCCGGCAGGTGGGGATCGACCCCGCGGCGACCGATGG
CGTTCATGCCGACCGGATCCGGCAGCTCGCCCGCGAGGGAAGCCGCCTGACCGCCCAGCACCTCCGGGCGCTCGGTCCGCTCCGCCGCCACGCCGTCCTG
GTCGTGACGGTGCTGGACACGATCGCTCGCCTCACCGACGAGGCCATCGGCCTCTTCGATCGGCTGATCGGACGGATGTTCCGGCGGGCCGAGCGGCGCG
CTTCGGCCGAACTCCGGAACAACGCCCGGTCCATGAACGACAAGGTGCGCCTGCTGGCCAAGCTCGGCGACGCCCTGATCGACGCCAGGAAAACCGGAAC
CGACGCCTTCGCCGCGATGGACAAGGTGATCCCCTGGGACCGGTTCGCCGAGGTCGTCGCGGAAGCCAGGGGGCTGACCCGGGACGATGGTCCGGACTAC
CTCGCGCTCGCCGAAGCGAACCATGCGCTGCTGCGGCGGGTCGGCCCGCTGTTCCTGGACGCCTTCGAGTTCCAGGGCATCGCCGCCGTGACCGGGCTGC
TCCGTGCTGTCGCGACGCTGCGTACCTTCTACGCCGGCAGTCGGCGAACCCTGCCGAAGGACATCCCGACCGGTTTCATCCGACGGAGCTGGCGGTCCGC
CGTCTTCCGCGACGGCGCCATCGATGGCCAAGCCTACGAGCTGTGCCTCTTCGCGGAGCTTCGGGACCGGCTGCGCGCCGGCGACGTCTGGGTGGTCGGC
AGCCGGCAGTACCGGGCCGTCGAGGATCAGCTCATTTCGAAGCCGCTGTTCGCCGAGATGCGGGAGGCCGGCCCGCTGCCGGTGGCGGTGGCGACCGACG
CCACGCGCTACCTCCAGGACCGGAAGGAGATGCTGGAAGCGCGGCTGCGCGAGGTCTGCGACAAGGCCGCCCGGGACCAGCTTGTGGATGTTCGCTTCAG
CGGCGACACCCTCAAGATCTCCCCGCTGCAAGCGGCGACGCCGGAAGACGCGGAACGGCTGGCCGACCGGGTCTACGCGTTGCTGCCGCGGGTGCGGATC
ACCGATCTCCTCGACGAGGTCGCCGAGTGGACCGGCGCCGTCGACTGCTTCACCCATCTGCGCACCGGCCTGCCGGCCGAGGACCGCCGCGTCGTCCTGA
CCGCCGTGTTGGCCGACGCCACCAATCTTGGACTGACGCGCATGACCGAGGCGTGCAGTGTCGCCAGTTACAAGCGGCTCGCCTGGACGGCGGGGTGGCA
TGTCAGCGAGGACGCCTACGGCCGGGCTCTGGCCCGGATCGTGGCGGCCCAGCAGGCGCAGCCCCTGGCCGCCCGCTTCGGCGGCGGAACGGCCTCCAGT
TCCGATGGCCAGCACTTCCCGCTCGGCGGGCGCGGCGAGATAACCGGGGCCGTCAACCCGCACAAGGGGTCCGGCCCGGCGATCTCCTTCTACACCCACG
TCTCGGACCGTTACGCGCCCTACCACACCAAGGCGATCTCGGTGGCGGAGAGCGAGGCGGCCCACGTCATCGACGGCCTGCTCTACCATGGCGCGGACAT
CGGGATCGCCGTCCACCATGTCGATGGAGGCGGCGTTTCGGACCATGTCTTCGCGCTGTGCCATCTCCTTGGCTTCCGCTTTGCGCCGCGCATCCCCAAC
CTGCACGATCGGCGCCTTTACACCTTCGGGCCGGCGGCAACCTGGCCGACCCTGGAGCCGTTCATCGCCGGGCGGATCGACGAGGCGCTGATCCAGGCGC
ACTGGGAAGACCTGCTGCGGCTGGCCACCTCGATTCGGACCGGCACCGTGCCGGCGTCGCTGATGCTGAAACGGCTCGGCTCCTACCCTCGGCAGAACGG
ATTGGCCTTGGCTCTCCGGGAGATCGGGCGCATCGAGCGGACAGCTTTCAGCTTGGAGTGGCTGCAAAATCCGGCACTTCGCCGCCAAGCCACGACCGAG
TTGAACAAGGGGGAGTCCCGCAACGCGCTCGCCCGCGCCGTGTGTTTCCACCGCCTTGGACGCATCCGCGACCGGACGCCGGAGAACCAGCAGCACCGCG
CCGGCGGCCTCAACCTGGTCGTGGCCGCGATCATCCTGTGGAACACCGTCTACATGGAGCGGGCCATCGATGTCCTGCGCGCCGGGGGTGAGCGCATTCC
CGACGAGTTGTTGAGCCATCTCGCGCCGCTGGGATGGCAACACATCAACCTGACCGGCGACTATCTCTGGAGTGCGGACCGGGTGCCGGGCACGTCGGGA
TACCGCCCGCTGCGCGCTCCAACCGAACTCCTGGCCGCATGATGTTCCGGCTTTGTCCGCTTAGCGTATATTCTCGCCCATTCTATGGCGTCACCCC

Protein section

ORF number : 3

ORF 1

Length		Begin	End	Strand	Fusion ORF
504 bp	167 aa	550	47	-	No

ORF function : Passenger Gene
Annotation : acetyltransferaseDescription :

ORF sequence :

MNSAVHLAPVLYGLPLGIDVLRAEAACEGHRFLERLVTEWISHRQRFAREGEVLLAAYVGSELAGIGGMTIDSAVMGALRMRRFYVRPSLRHRGIGRKLA
LSLLERSQSLECQVMVNAGTADASAFWEALGFMPDPCNGHTHIFHPRNSCWRPHVSPSGGAISRERS

Blast result :

ORF 2

Length		Begin	End	Strand	Fusion ORF
225 bp	74 aa	771	547	-	No

ORF function : Accessory Gene
AG : Tn3 resolvase

ORF sequence :

MGQRAALYCRVSTADQSCARQERDLLAFAARADYQVVGVFKGRSYRLIGRELGLSKNTVTDIVKRHRDVAEANE

Blast result :

ORF 3

Length		Begin	End	Strand	Fusion ORF
2976 bp	991 aa	867	3842	+	No

ORF function : Transposase
Chemistry : DDE

ORF sequence :

MPRRRVLTDAQITALFALPTAEADLIRHYTLGPDDLAVIARRRRPHNRLGFALQLCALRYPGRLIRPGELVPPTIVAFLAEQLDIDPDTLADYAARAPTR
YDQLDALHEAFGFSTFTQPDHRELSAWLVPIALATVSAITVAEALMAELRRRRIAAPGTTTIERMVATALLLAERHAAAKLNSTLSPERCTALDALLDNH
PGTAVSSLAWTRQPPGAPGHRSMARLVDQLRHLRQVGIDPAATDGVHADRIRQLAREGSRLTAQHLRALGPLRRHAVLVVTVLDTIARLTDEAIGLFDRL
IGRMFRRAERRASAELRNNARSMNDKVRLLAKLGDALIDARKTGTDAFAAMDKVIPWDRFAEVVAEARGLTRDDGPDYLALAEANHALLRRVGPLFLDAF
EFQGIAAVTGLLRAVATLRTFYAGSRRTLPKDIPTGFIRRSWRSAVFRDGAIDGQAYELCLFAELRDRLRAGDVWVVGSRQYRAVEDQLISKPLFAEMRE
AGPLPVAVATDATRYLQDRKEMLEARLREVCDKAARDQLVDVRFSGDTLKISPLQAATPEDAERLADRVYALLPRVRITDLLDEVAEWTGAVDCFTHLRT
GLPAEDRRVVLTAVLADATNLGLTRMTEACSVASYKRLAWTAGWHVSEDAYGRALARIVAAQQAQPLAARFGGGTASSSDGQHFPLGGRGEITGAVNPHK
GSGPAISFYTHVSDRYAPYHTKAISVAESEAAHVIDGLLYHGADIGIAVHHVDGGGVSDHVFALCHLLGFRFAPRIPNLHDRRLYTFGPAATWPTLEPFI
AGRIDEALIQAHWEDLLRLATSIRTGTVPASLMLKRLGSYPRQNGLALALREIGRIERTAFSLEWLQNPALRRQATTELNKGESRNALARAVCFHRLGRI
RDRTPENQQHRAGGLNLVVAAIILWNTVYMERAIDVLRAGGERIPDELLSHLAPLGWQHINLTGDYLWSADRVPGTSGYRPLRAPTELLAA

Blast result :

Comments

ISAzs29 is 71% (ORFC : the transposase) aa similar to ISMpo10.
The first ORF is a passenger gene annotated as acetyltransferase, the second is the Tn3 family resolvase with an internal deletion (between aa 43 and 172) and the third is the transposase.

References

1] ISfinder annotation (2011)
2] Kaneko,T., Minamisawa,K., Isawa,T., Nakatsukasa,H., Mitsui,H., Kawaharada,Y., Nakamura,Y., Watanabe,A., Kawashima,K., Ono,A., Shimizu,Y., Takahashi,C., Minami,C., Fujishiro,T., Kohara,M., Katoh,M., Nakazaki,N., Nakayama,S., Yamada,M., Tabata,S. and Sato,S. (2010) DNA Res. 17 (1), 37-50.