ISAzs28

  • Family IS5
  • Group IS1031
MGE type ISRelated element(s) :
Isoform Synonym(s)
Accession numberTranspositionOriginHost
NC_013855 ND Azospirillum sp.
Azospirillum sp. B510 plasmid pAB510a
DNA section
IS Length : 1055 bp

Ends


IR Length : 19/25

IRL : CAGCCTGATCCGAAATTAGATCGGATCGCGTAAAATCGGAATCGATTTGA
IRR : GAGTCTGATTCGAAAATAATCAGAACTGTATCAACGCCTTGCGATATGGT

Insertion site


Left flankDirect repeatRight flankDR Length
CTCGTACCGCTCCCGCCTTATAATGCCGTCGAAGA0

DNA sequence

CAGCCTGATCCGAAATTAGATCGGATCGCGTAAAATCGGAATCGATTTGAAGCGTGAATCCGATCGTCAGACATAAAGCTATAGCGTTGTGCGTTTCATA
TTAAACGCACAACGCTATAAGTTGAGTGGTATCAGTCGGTTATCTTGACAGCGTGGCCTTTCGGTGATTCAGGGTTTTCGCCAAAAACAACCGTGCATCC
CCGATGTGGACTGATACCACCCGCGCTCTTCATGCCCGTAGCGGTCTGGCTTTGCCAAGCGATTTGACTGATGCTGAATGGTCTTTGTTGGAACCGCTGC
TCCCGCCCGCGTCCTCCGTAGGGCGTCCGCGTAAATGGCCGCTGCGCCGGATCGTCAAAGCGACCCTTTACCTGCTTCGCGGCGGCCTGCCGTGGCGGAT
GCTGCCGCCCTGCTTTCCGCCGGTCTCGACGGTGCGGCGCTGGTTCTACCTGTGGCGCGACAACGGCCTCTGGCTCACGCTCAATCATGTTCTGCTGATG
GCAATGCGTGAGGCACAGGGGCGCGAAGCCAGCCCGAGTGCCGGCGTGATCGACAGCCAGAGCGTCAAAACCACGGAAGCCGGTGGTCCACGCGGCTATG
ATGCCGGCAAAAAGATCAAGGGGCGTAAGCGCCATATCCTCACCGACACCCAGGGCAATCTCGTCCATGCCGTGATCCACGCCGCCGATATCCAGGATCG
TGACGGCGCGCCGCTGGTGCTCGCCGAAATCGTCAAACCCTTCCCATGGCTGCGGCACGTCTTCGCCGATGGCGGCTATGCCAGAGACAAGCTCAAAGAG
GCGCTGCGCCGGATCGGAAAATGGACCATCGACATCATCAAGCGATCCGACACAGCCAAAGGCTTCGAGGTCTTGCCCCGGCGCTGGGTCGTGGAGCGTA
CCCTGGCATGGCTTAACCGTAACCGACGGCTCGCAAAGGACTTCGAACACACCATCGCTTCCGCTACCGCATGGTGCTTCATCGTATCCGTTCAACTCTT
CGTTCACCATATCGCAAGGCGTTGATACAGTTCTGATTATTTTCGAATCAGACTC
Protein section
ORF number : 1

 

ORF 1
LengthBeginEndStrandFusion ORF
822 bp273 aa2041025+No
ORF function : Transposase
Chemistry : DDE

ORF sequence :

MWTDTTRALHARSGLALPSDLTDAEWSLLEPLLPPASSVGRPRKWPLRRIVKATLYLLRGGLPWRMLPPCFPPVSTVRRWFYLWRDNGLWLTLNHVLLMA
MREAQGREASPSAGVIDSQSVKTTEAGGPRGYDAGKKIKGRKRHILTDTQGNLVHAVIHAADIQDRDGAPLVLAEIVKPFPWLRHVFADGGYARDKLKEA
LRRIGKWTIDIIKRSDTAKGFEVLPRRWVVERTLAWLNRNRRLAKDFEHTIASATAWCFIVSVQLFVHHIARR

 

Blast result :
Comments
ISAzs28 is 78% aa similar to ISAli1.
References
1] ISfinder annotation (2011)
2] Kaneko,T., Minamisawa,K., Isawa,T., Nakatsukasa,H., Mitsui,H., Kawaharada,Y., Nakamura,Y., Watanabe,A., Kawashima,K., Ono,A., Shimizu,Y., Takahashi,C., Minami,C., Fujishiro,T., Kohara,M., Katoh,M., Nakazaki,N., Nakayama,S., Yamada,M., Tabata,S. and Sato,S. (2010) DNA Res. 17 (1), 37-50.