ISfinder Mirror Site

ISAzs27

Family IS30
Group

MGE type ISRelated element(s) :
Isoform Synonym(s)

Accession number	Transposition	Origin	Host
NC_013857	ND	Azospirillum sp.	Azospirillum sp. B510 plasmid pAB510c

DNA section

IS Length : 1270 bp

Ends

IR Length : 14/23

IRL : ATGCGTTTTTACTGTGTAGGCGGGGTAGGTTCTATTCTGAAGTGCAACTT
IRR : ACACGTTTTTGTTGATCGGGGGGCCAGCTTTTTAGCCATCATGTTGGGAG

Insertion site

Left flank	Direct repeat	Right flank	DR Length
CATAATTTCCGTT		CGCACTTCAG	0

DNA sequence

ATGCGTTTTTACTGTGTAGGCGGGGTAGGTTCTATTCTGAAGTGCAACTTTCCTGTAATCACAGGGCTTTACGGGTGGTGAGCGCTGATGGGTGAGCATT
ATTCGCAGATCGATTTCGCCGAGCGCCGCCGCATCCAGGATATGCGCGACGCCAAGATGCCGGTGACGGCGATAGCCGAGGCTTCGGGTCGTCACCGGTC
GACCATCCACCGGGAAATCCGGCGCAACTTCTATCACGACCCGTTTCGAGATCGCTGGGGCAAAGAGTACAAGGGCTACTTCTGCACCAGCGCCCAGAAG
TATGCCCATGATCAACGCTCTCGTCGTAGATTGAAACTGACGCTCAAGCCGGTTCTGCGCGACCACGTGATCGCCAAGCTGCGGGAGGGCTGGTCGCCAC
AACAGATTGCTGGCCGCCTCAAGTTGGATGCTTCTGATAGCGCGACAGTGTCGCACGAGACGATTTACCGCTTCATCTACGGTCCAGAAGGGCGAACCCT
TAATCTCTATGCGCTTTTGGCCGTCGCCCGGCGTCGGCGTCGCAAGCGCATGGGGCGCAAACCACGCAACAGTCCGATCCCCGCTGAGCGCTCAATCGCC
AATCGTCCGGCCGAGGTGGAGACCCGGCAGAGTTTCGGGCACTGGGAATGTGACTTGGTGATCTTCAGCCCTCAGCTCGGCAAATCGAACATCACGTCTC
TTCAGGAACGTCAGAGCCGATACCACATCCTCATTGCCAATGAGGATCGCCGCTCAGCCGGGGTGATTAGCCGGATCGGAACGGTGCTTGGCAAATTTCC
ACATCATGCATGCCGAACCATTACTTTTGACCGGGGAACGGAGTTCCTATCATACAAAAGCCTATCGTTGGACAGTTATTTTTGCAATCCGCACAGCCCC
TGGCAAAAGGGTGGTGTTGAGAATGCCAACGGACGTTTGCGGCGATATTTGCCTCTGGGCGTCCCTGTAGCGGAGCGCAATGGCTCAGCACTTGCCGCCC
TCGCGAAGCGGCTCAACGATACGCCACGCCGCTGCCTTGACTACAGGACGCCAGCCGAAGTCTTTGGTGAATACCAGCTACGCCTGAGCGCCATGAGAGG
CCGTGAAACCAGCAGACCTCCCCTGCCCCGCCGCTCGCACCGGTGGGTCCACAGGCTTGATCCGCCATCGCGATGGAGGCGCGCGGATCAAGCCTATGGA
CTACCGGTACGAGCTCTCCTCTCCCAACATGATGGCTAAAAAGCTGGCCCCCCGATCAACAAAAACGTGT

Protein section

ORF number : 1

ORF 1

Length		Begin	End	Strand	Fusion ORF
1152 bp	383 aa	88	1239	+	No

ORF function : Transposase
Chemistry : DDE

ORF sequence :

MGEHYSQIDFAERRRIQDMRDAKMPVTAIAEASGRHRSTIHREIRRNFYHDPFRDRWGKEYKGYFCTSAQKYAHDQRSRRRLKLTLKPVLRDHVIAKLRE
GWSPQQIAGRLKLDASDSATVSHETIYRFIYGPEGRTLNLYALLAVARRRRRKRMGRKPRNSPIPAERSIANRPAEVETRQSFGHWECDLVIFSPQLGKS
NITSLQERQSRYHILIANEDRRSAGVISRIGTVLGKFPHHACRTITFDRGTEFLSYKSLSLDSYFCNPHSPWQKGGVENANGRLRRYLPLGVPVAERNGS
ALAALAKRLNDTPRRCLDYRTPAEVFGEYQLRLSAMRGRETSRPPLPRRSHRWVHRLDPPSRWRRADQAYGLPVRALLSQHDG

Blast result :

Comments

ISAzs27 is 58% aa similar to ISRle2.

References

1] ISfinder annotation (2011)
2] Kaneko,T., Minamisawa,K., Isawa,T., Nakatsukasa,H., Mitsui,H., Kawaharada,Y., Nakamura,Y., Watanabe,A., Kawashima,K., Ono,A., Shimizu,Y., Takahashi,C., Minami,C., Fujishiro,T., Kohara,M., Katoh,M., Nakazaki,N., Nakayama,S., Yamada,M., Tabata,S. and Sato,S. (2010) DNA Res. 17 (1), 37-50.