ISfinder Mirror Site

ISAzs26

Family ISKra4
Group ISAzba1

MGE type ISRelated element(s) :
Isoform Synonym(s)

Accession number	Transposition	Origin	Host
NC_013854	ND	Azospirillum sp.	Azospirillum sp. B510

DNA section

IS Length : 2226 bp

Ends

IR Length : 22

IRL : GAGAGCAGAACAATCCTGGGGGACAGGCGATAGGCGCGGCCGGCGTGCTG
IRR : GAGAGCAGAACAATCCTGGGGGGAGCGGCATCAGGCGGCCGCTTGATCCC

Insertion site

Left flank	Direct repeat	Right flank	DR Length
GCCCAGCAGG		CCAGTTGGAA	0

DNA sequence

GAGAGCAGAACAATCCTGGGGGACAGGCGATAGGCGCGGCCGGCGTGCTGCGGTAGAGTGTGGCAATCACCGCTTGCCGAGAGACCATGGCGCGCCGCCC
CGTCAAAAAGCCGAAGCCCGCACCGGATCTGCCAATCCTGCAGCTCAAGGTCCGTCTGCTGGACATCAGCCCCTCGGCGTGGCGCCGGCTGCTGGTGCAG
GCCTCCACCACCTTGCAGGAACTGCATGGTATCCTCCAGGTCGCCCTGGGCTGGGAAGGCATCCACCTCTACCGGTTCGATCTTCGCGCGGTTGGGTATG
GGTCGACAGAGTTGGCCCTCGTCTCACCCCGGGTTGTCCTCGACACCTTCCGCCTGCGGATCGGCGGCAAGTTGCGCTACACCTACGACATGGGCGCCTT
CTGGCGTCACGAACTTCGGGTGGAGGACCGGCTCGCCGCGCAGCCCGACGCCGTCTACCCGGTCTGCATCGGCGGGGAACACCCTTGTCCTCCGGAGGGC
TGTGGCGGGCCAGCGGGTTACCGGGAGCGTCAGGACGATCTGGTGGGCTGGGACGCGTGGGAGGACTTGGCGGAGATGACGGAGGTGCTCGCCGCCGTCG
CCCGTGACCGGACCACCGCGGTGCTGGACGATCCCGACGTCCGCGATCGTTTCGAGCGGACCCTCGCGCGCACCCGGGCGCGCGAGCGTTTCCGGCCCAA
GGAGTTCAGCCGAATCCAGATCAATGAGCGCTTCCGCGCCGAGGAGCACAAGCCGCTGATGCACCAGCAGGTCTGGTGAGCCGGAGGCGGAGATGAGGAT
GCGGATCAGGGTACGGCTGCAGATCGAGGACGAGAACGGGACCGTGGTCGCCGACGACGAGGTTCTCGGGTTGGCCAAGCCGGCCGACCGGGTGGAAGAA
CTCGGCTTGTCGCTGGAGGAGGCCAAGCGGATGTTGCGGGCGACCCAGGAGCGCCTGCTCGCTGCCCAAGCGGCCGACTACACCGCCCGCCACCGGTGCT
GCCCGGATTGCGGGTGCGTCCGCACCAGCAAGGGGCTGCGGACGATCCGGTTCCGCACCTTGTTCGGCACGGTGGACGTGCCTGGCCGGCGTCTGCATCG
GTGTGGCTGCGACGGGAAAGGCGGCGGAAGCGTCAGCCCGTTGCGCGCTCTGTTCACCGAGCGCGCGGCGCCGGAGCTGCTGTTCCTGGAGGCCAAGTGG
GCCTCGCTGGTGTCGTATGGAATCACCACCGATTTGTTGAAGGATGTGCTGCCGATCGCCGATACGACCAATCCGACCACCGTTCGCCGCGACCTGTCCC
GGGTCGCCGACCGCCTGGAGGCCGCTCTCGGGGAGGAGCGCTTCTCTCTGATCGACGGATGTCCGGCGGACTGGCGGGAGTTGCCGGCCCCGGAGGGGCC
GATCGTCGTCGGTGTGGATGGCGGGTACGTTCGCAGCTGGGACGACAAAACGACCAGCTTCGAGGTGATCGCCGGCAAGGCGGTGCCGGAGGAGCGGGTC
GACCGGTTCTTCGGCTTCGTGCAGACGCACGACAAAAAGCCGCGGCGCCGCCTGCACGAGGTGCTGCGCGGTCAGGGATTCCAGATGAATCAGGACCTCA
CCTTCCTCACCGACGGAGGTGACACCGTGCGCACTTTGGTCACCGAACTGCCGCCCTGCGCCGAGCATATCCTCGACTGGTTCCACGTCACCATGCGGCT
GACCGTGCTCGGGCAGTTCGCCAAAGGATTGGCCCACCACGACGAGGCCAAGGCCGCTGCCATCGCGGAGCGGTTGGAGGGCATCAAGTGGTGGCTGTGG
CATGGCGATGGCCGGGAGGCTCAGCAGCGGATCGAAGGCTTGGCCGACGATCTTCGAGACCTCACCGTTCCTTACGATGGGCTCAAGAAATTCACCCGGC
TGACGGCGGAGTTCGCCACGTACATCGCCAACAACCTGGATTCCATCCCGAACTATGGGGAACGGTGGCGCTATGGCGAGCGCATCTCGACGGCCTTCGT
GGAGTCGACCGTCAACGTGGTGATCGACAAGCGGATGTCCAAACGCCAGCAGATGCAATGGACCAAGCAAGGCGCTCACCGTCTGCTCCAGATCCGCACC
CGGACGCTCGACGGCACGCTCCGGCCCACCTTCGAGGGCTGGTTCCCCGGTCTGGCGGCGAACGATAATGCCCGAGGGGATCAAGCGGCCGCCTGATGCC
GCTCCCCCCAGGATTGTTCTGCTCTC

Protein section

ORF number : 2

ORF 1

Length		Begin	End	Strand	Fusion ORF
720 bp	239 aa	60	779	+	No

ORF function : Passenger Gene
Annotation : plasmid pRIA4b ORF-3 family proteinDescription :

ORF sequence :

MAITACRETMARRPVKKPKPAPDLPILQLKVRLLDISPSAWRRLLVQASTTLQELHGILQVALGWEGIHLYRFDLRAVGYGSTELALVSPRVVLDTFRLR
IGGKLRYTYDMGAFWRHELRVEDRLAAQPDAVYPVCIGGEHPCPPEGCGGPAGYRERQDDLVGWDAWEDLAEMTEVLAAVARDRTTAVLDDPDVRDRFER
TLARTRARERFRPKEFSRIQINERFRAEEHKPLMHQQVW

Blast result :

ORF 2

Length		Begin	End	Strand	Fusion ORF
1404 bp	465 aa	793	2196	+	No

ORF function : Transposase
Chemistry : DDE

ORF sequence :

MRIRVRLQIEDENGTVVADDEVLGLAKPADRVEELGLSLEEAKRMLRATQERLLAAQAADYTARHRCCPDCGCVRTSKGLRTIRFRTLFGTVDVPGRRLH
RCGCDGKGGGSVSPLRALFTERAAPELLFLEAKWASLVSYGITTDLLKDVLPIADTTNPTTVRRDLSRVADRLEAALGEERFSLIDGCPADWRELPAPEG
PIVVGVDGGYVRSWDDKTTSFEVIAGKAVPEERVDRFFGFVQTHDKKPRRRLHEVLRGQGFQMNQDLTFLTDGGDTVRTLVTELPPCAEHILDWFHVTMR
LTVLGQFAKGLAHHDEAKAAAIAERLEGIKWWLWHGDGREAQQRIEGLADDLRDLTVPYDGLKKFTRLTAEFATYIANNLDSIPNYGERWRYGERISTAF
VESTVNVVIDKRMSKRQQMQWTKQGAHRLLQIRTRTLDGTLRPTFEGWFPGLAANDNARGDQAAA

Blast result :

Comments

ISAzs26 is 47% (ORFB : the transposase) aa similar to ISAzba1. The first ORF is a passenger gene annotated as plasmid pRIA4b ORF-3 family protein.

References

1] ISfinder annotation (2011)
2] Kaneko,T., Minamisawa,K., Isawa,T., Nakatsukasa,H., Mitsui,H., Kawaharada,Y., Nakamura,Y., Watanabe,A., Kawashima,K., Ono,A., Shimizu,Y., Takahashi,C., Minami,C., Fujishiro,T., Kohara,M., Katoh,M., Nakazaki,N., Nakayama,S., Yamada,M., Tabata,S. and Sato,S. (2010) DNA Res. 17 (1), 37-50.