ISAzs25

  • Family IS1380
  • Group
MGE type ISRelated element(s) :
Isoform Synonym(s)
Accession numberTranspositionOriginHost
NC_013856 ND Azospirillum sp.
Azospirillum sp. B510 plasmid pAB510b
DNA section
IS Length : 1614 bp

Ends


IR Length : 16/20

IRL : CCGGGATTATGCACCGGGGTTGGCGGGTGTAGCGCAAGCCGTTGAACAGG
IRR : CCGCCTTTATGCACCTGGGTGAATCGTCATTTGGCCGCTGAATGGGGATG

Insertion site


Left flankDirect repeatRight flankDR Length
GATACGGCGAGTGCTAACTAGCCGGGAAAATTCATG0

DNA sequence

CCGGGATTATGCACCGGGGTTGGCGGGTGTAGCGCAAGCCGTTGAACAGGCATAGGATTTGGGTGCTGAAGCCAATCCCCTCCGTATCGGGAGCGCCCAC
CCGCCATGGTTGATCCTACGCTGCCGTTGCCCAGCTTGTCACCGGTTGCCGACAAGGCCGTCGTCGGGCGCTTCGATGGTGGCCGCCTGTCCTGCGATGG
CGGACTTCTGGTGCTGCGCGAAGTGGAAAAGCGGCTGCGCGTGGCCGAGCGTCTGGCCGGCAGCATCGAGGACCCGCGCGATCCGGTGCGCACCACGCAT
TCCCTGGCCGATATCATCGGCTTCCGCCTGCTGGCCATCGCGGCGGGCTACGAGGACGGCAACGACGCTGACGATCTGCGCGCCGATCCGCTGTTCAAGC
TGGCCCTGGACCGGTTGCCGTCGGAGCGCGACCTGTGCTCCCAATCTACCATCTCGCGGCTGGAGAACCTGCCCGACCCCCGCGCGCTGCTGCGCATGGG
GCGGGCCATGGTCGATCTCTACTGCGCTTCGTTCCGTCAGGTGCCCAAGCGCATCGTGCTGGACGTGGACGACACCTTCGACACCGTGCACGGTGATCAG
CAGTTGCGCCTGTTCAACGCTCATTATGACGAGTACGGCTTCCAGCCCATGGTCGTCTTCGACGGCGATGGGCGCTTCGTCACCGCCGTGCTGCGCCCGG
CCAAGCGGCCCAAGGGTACAGAGATCCGGGCCTTCCTGCGCCGTCTGCTCCGCGCCATCCGGGCGAACTGGCCCAGGACCGAGATCCTGCTGCGCGCTGA
CGGCCACTACGCCTGCCCCGAGGTTCTGGACTGGTGTGAGGCCGAGGGCCTCGACTACATTCTGGGCCTGCCGACCAGCAGCACGCTGCGCCGTCACGTC
ACCACGCTGGAGGTCAGCACCGCTGCCCGCTTCCAGGCCATGCCGGGGACCGACAAGCTGCGTCGCTTCAAGGAATTCTACGACGGCGCCGGCACCTGGA
GCCGGGTCCGCCGCATCGTCGCGCGGGTCGAGGCCGGCCCCCAGGGCACCGACACCCGTTTCATCGTCACCAATCTACGCCACGGGACCGGACGCGCTCT
GTACGAGGGCGTGTACTGCGCCCGCGGCCAAGCCGAGAACCACATCAAGGCCTGGAAAGCCCACCTCGCGGCCGACCGCACCTCCTGCACGAAAGCGACC
GCCAACCAGTTCCGCCTGTTCCTGCACGCCGGGGCCTACTGGCTGCTGTGGAGCCTGCGCTCGCTGATGCCCAAACGCTCCCGCTGGCGGGTCGCCCAGT
TCGACACCCTGCGGCTGCGCATGGTGAAGATCGCCGCGCGCATCATCGAGAGGAAGACGCGCATCACGGTCCACCTGCCGAGTGCCGCGCCCGATCAGGC
GATCGTTCATCTCGCCCTCGGCCGCCTGCCTCGGCTCATCTCCTGACCACAGGGGCAACTGCCCCAGCCGTCACTCCATCCCATCAACCACTAAACCCAC
ACCCATCAAAACACCGCCATCAGCGCGGCGTCGACGGCGTGCACCTTCGCAACCTCCCGATGAACATCCCCATTCAGCGGCCAAATGACGATTCACCCAG
GTGCATAAAGGCGG
Protein section
ORF number : 1

 

ORF 1
LengthBeginEndStrandFusion ORF
1341 bp446 aa1061446+No
ORF function : Transposase
Chemistry : DDE

ORF sequence :

MVDPTLPLPSLSPVADKAVVGRFDGGRLSCDGGLLVLREVEKRLRVAERLAGSIEDPRDPVRTTHSLADIIGFRLLAIAAGYEDGNDADDLRADPLFKLA
LDRLPSERDLCSQSTISRLENLPDPRALLRMGRAMVDLYCASFRQVPKRIVLDVDDTFDTVHGDQQLRLFNAHYDEYGFQPMVVFDGDGRFVTAVLRPAK
RPKGTEIRAFLRRLLRAIRANWPRTEILLRADGHYACPEVLDWCEAEGLDYILGLPTSSTLRRHVTTLEVSTAARFQAMPGTDKLRRFKEFYDGAGTWSR
VRRIVARVEAGPQGTDTRFIVTNLRHGTGRALYEGVYCARGQAENHIKAWKAHLAADRTSCTKATANQFRLFLHAGAYWLLWSLRSLMPKRSRWRVAQFD
TLRLRMVKIAARIIERKTRITVHLPSAAPDQAIVHLALGRLPRLIS

 

Blast result :
Comments
ISAzs25 is 94% aa similar to ISAzs3.
References
1] ISfinder annotation (2011)
2] Kaneko,T., Minamisawa,K., Isawa,T., Nakatsukasa,H., Mitsui,H., Kawaharada,Y., Nakamura,Y., Watanabe,A., Kawashima,K., Ono,A., Shimizu,Y., Takahashi,C., Minami,C., Fujishiro,T., Kohara,M., Katoh,M., Nakazaki,N., Nakayama,S., Yamada,M., Tabata,S. and Sato,S. (2010) DNA Res. 17 (1), 37-50.