ISAzs24
- Family IS3
- Group IS51
Isoform Synonym(s)
| Accession number | Transposition | Origin | Host |
|---|---|---|---|
| NC_013856 | ND | Azospirillum sp. | Azospirillum sp. B510 plasmid pAB510b Azospirillum sp. B510 plasmid pAB510f Azospirillum sp. B510 plasmid pAB510a Azospirillum sp. B510 Azospirillum sp. B510 plasmid pAB510c |
DNA section
IS Length : 1325 bp
Ends
IR Length : 23/27
IRL : TGAACCGCACCGGGTTTTCTGGAGGCCCCGTTTCTTGAGAGACTGGGTTC
IRR : TGAACCGCGCCGGTTTTCCCGGAGGCCGGTTTGGTTGAGTCACGCCGCCA
Insertion site
| Left flank | Direct repeat | Right flank | DR Length |
|---|---|---|---|
| CTTGCTGGCTGCGAC | ATTCGCCGCTGCCAT | 0 | |
| CGGAGCAGTTCACTG | CCTCTTTCGAACAGG | 0 |
DNA sequence
TGAACCGCACCGGGTTTTCTGGAGGCCCCGTTTCTTGAGAGACTGGGTTCAACATGACGAAGCAAGCATCACCCAAATACGCGCCTGAAGTGCGCGAACG
CGCGGTTCGCATGGTGTTTGACCACGAAGGCGAACACGCGTCGCAGTGGGCTGCGATCGGCTCGATCGCTGCGAAGATCGGCTGCACGGCGGAGACGTTG
CGGGGCTGGGTCCGGCAGGCCGAACGTGACCAGGGCAAGCGGCCCGGTCCGACGACGGACGAGCAGGAGCGGATCAAGGCGCTGGAGCGTGAGAATCGCG
AACTGCGCCAAGCGAACGAGATTCTGCGCAAGGCGTCGGCGTATTTTGCTCAGGCGGAGCTCGACCGCCCGTTCAAGAAATGATCGCCTTCATCGACGAG
CATCGCGCCGTCCACGGGGTCGAGCCGACCTGCAAAGTGCTGCCGATCGCCCCGTCGAACCTACCGCGCCCATGCCGCCCGGCGGGCCGATCCGGCCAAG
GCGCCGGCCCGCTCGCGAAGCGACGCGGAGCTGAGTTTGGCTATCCGACGGGTCTGGAATGAAAACTTCCAGGTCTACGGGGTGAGGAAAGTGTGGCGGC
AGTTGCGGCGGGAGGGCTTCGACGTGGCGCGCTGCACGGTGGCTCGCCTGATGCGGTCCATGGGCTTGAAGGGGGCGATGCGCGGCAAGGCGGTGCGCAC
CACGATCAGCGACAAGGCGGCATCTTGCCCGCTCGACCGGGTGAACCGCCAGTTCCAGGCTTCTCGTCCGAATGCCTTGTGGGTGGCCGATTTCACCTAC
GTTGCCACATGGCAGGGCTTCGTCTACGTCGCCTTCGTCATCGACACCTTCGCCCGCCGCATCGTGGGCTGGCGGGTGTCGCGCACCGCCCACGCCGACT
TCGTCCTGGATGCTTTGGAGCAGGCTCTGCATGACCGCCGGCCGACCAAGGGCAGCGGCCTCGTCCATCATTCCGATCGTGGATCGCAATATCTCGCCAT
TCGCTACACCGAACGCCTCACCGAAGCCGGCGTCGAGCCCTCCGTCGGCAGCATCGGCGATTCCTATGACAACGCTTTGGCCGAGACCATCAACGGCCTT
TACAAGACCGAGGTGATCCGCCGCCGCGGGCCGTGGCGCACCCTGGAGGCTGTCGAGTTCGCTACCCTGGAATGGGTGGACTGGTTCAACCACCGACGCC
TCCTCGAACCCATCGGCAACATTCCTCCCGCCGAGGCCGAAGCACGCTATTATGCCCAAACCGAGGACGTCCCTATGGCGGCGTGACTCAACCAAACCGG
CCTCCGGGAAAACCGGCGCGGTTCA
CGCGGTTCGCATGGTGTTTGACCACGAAGGCGAACACGCGTCGCAGTGGGCTGCGATCGGCTCGATCGCTGCGAAGATCGGCTGCACGGCGGAGACGTTG
CGGGGCTGGGTCCGGCAGGCCGAACGTGACCAGGGCAAGCGGCCCGGTCCGACGACGGACGAGCAGGAGCGGATCAAGGCGCTGGAGCGTGAGAATCGCG
AACTGCGCCAAGCGAACGAGATTCTGCGCAAGGCGTCGGCGTATTTTGCTCAGGCGGAGCTCGACCGCCCGTTCAAGAAATGATCGCCTTCATCGACGAG
CATCGCGCCGTCCACGGGGTCGAGCCGACCTGCAAAGTGCTGCCGATCGCCCCGTCGAACCTACCGCGCCCATGCCGCCCGGCGGGCCGATCCGGCCAAG
GCGCCGGCCCGCTCGCGAAGCGACGCGGAGCTGAGTTTGGCTATCCGACGGGTCTGGAATGAAAACTTCCAGGTCTACGGGGTGAGGAAAGTGTGGCGGC
AGTTGCGGCGGGAGGGCTTCGACGTGGCGCGCTGCACGGTGGCTCGCCTGATGCGGTCCATGGGCTTGAAGGGGGCGATGCGCGGCAAGGCGGTGCGCAC
CACGATCAGCGACAAGGCGGCATCTTGCCCGCTCGACCGGGTGAACCGCCAGTTCCAGGCTTCTCGTCCGAATGCCTTGTGGGTGGCCGATTTCACCTAC
GTTGCCACATGGCAGGGCTTCGTCTACGTCGCCTTCGTCATCGACACCTTCGCCCGCCGCATCGTGGGCTGGCGGGTGTCGCGCACCGCCCACGCCGACT
TCGTCCTGGATGCTTTGGAGCAGGCTCTGCATGACCGCCGGCCGACCAAGGGCAGCGGCCTCGTCCATCATTCCGATCGTGGATCGCAATATCTCGCCAT
TCGCTACACCGAACGCCTCACCGAAGCCGGCGTCGAGCCCTCCGTCGGCAGCATCGGCGATTCCTATGACAACGCTTTGGCCGAGACCATCAACGGCCTT
TACAAGACCGAGGTGATCCGCCGCCGCGGGCCGTGGCGCACCCTGGAGGCTGTCGAGTTCGCTACCCTGGAATGGGTGGACTGGTTCAACCACCGACGCC
TCCTCGAACCCATCGGCAACATTCCTCCCGCCGAGGCCGAAGCACGCTATTATGCCCAAACCGAGGACGTCCCTATGGCGGCGTGACTCAACCAAACCGG
CCTCCGGGAAAACCGGCGCGGTTCA
Protein section
ORF number : 1
ORF 1
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 1233 bp | 410 aa | 54 | 1286 | + | No |
Chemistry : DDE
ORF sequence :
MTKQASPKYAPEVRERAVRMVFDHEGEHASQWAAIGSIAAKIGCTAETLRGWVRQAERDQGKRPGPTTDEQERIKALERENRELRQANEILRKASAYFAQ
AELDRPFKK*SPSSTSIAPSTGSSRPAKCCRSPRRTYRAHAARRADPAKAPARSRSDAELSLAIRRVWNENFQVYGVRKVWRQLRREGFDVARCTVARLM
RSMGLKGAMRGKAVRTTISDKAASCPLDRVNRQFQASRPNALWVADFTYVATWQGFVYVAFVIDTFARRIVGWRVSRTAHADFVLDALEQALHDRRPTKG
SGLVHHSDRGSQYLAIRYTERLTEAGVEPSVGSIGDSYDNALAETINGLYKTEVIRRRGPWRTLEAVEFATLEWVDWFNHRRLLEPIGNIPPAEAEARYY
AQTEDVPMAA
AELDRPFKK*SPSSTSIAPSTGSSRPAKCCRSPRRTYRAHAARRADPAKAPARSRSDAELSLAIRRVWNENFQVYGVRKVWRQLRREGFDVARCTVARLM
RSMGLKGAMRGKAVRTTISDKAASCPLDRVNRQFQASRPNALWVADFTYVATWQGFVYVAFVIDTFARRIVGWRVSRTAHADFVLDALEQALHDRRPTKG
SGLVHHSDRGSQYLAIRYTERLTEAGVEPSVGSIGDSYDNALAETINGLYKTEVIRRRGPWRTLEAVEFATLEWVDWFNHRRLLEPIGNIPPAEAEARYY
AQTEDVPMAA
Blast result :
Comments
ISAzs24 is 86% aa similar to ISAli4.
All signal for frameshifting are present but there is no frameshift in the transposase, there is a STOP codon in frame.
All signal for frameshifting are present but there is no frameshift in the transposase, there is a STOP codon in frame.
References
1] ISfinder annotation (2011)
2] Kaneko,T., Minamisawa,K., Isawa,T., Nakatsukasa,H., Mitsui,H., Kawaharada,Y., Nakamura,Y., Watanabe,A., Kawashima,K., Ono,A., Shimizu,Y., Takahashi,C., Minami,C., Fujishiro,T., Kohara,M., Katoh,M., Nakazaki,N., Nakayama,S., Yamada,M., Tabata,S. and Sato,S. (2010) DNA Res. 17 (1), 37-50.
2] Kaneko,T., Minamisawa,K., Isawa,T., Nakatsukasa,H., Mitsui,H., Kawaharada,Y., Nakamura,Y., Watanabe,A., Kawashima,K., Ono,A., Shimizu,Y., Takahashi,C., Minami,C., Fujishiro,T., Kohara,M., Katoh,M., Nakazaki,N., Nakayama,S., Yamada,M., Tabata,S. and Sato,S. (2010) DNA Res. 17 (1), 37-50.