ISAzs23
- Family IS3
- Group IS3
Isoform Synonym(s)
| Accession number | Transposition | Origin | Host |
|---|---|---|---|
| NC_013856 | ND | Azospirillum sp. | Azospirillum sp. B510 plasmid pAB510b Azospirillum sp. B510 plasmid pAB510f Azospirillum sp. B510 plasmid pAB510a |
DNA section
IS Length : 1596 bp
Ends
IR Length : 20/30
IRL : CCCTCTGTGCCAATGAGAGTGAGACACCGTGCCCCCTGAAGTTTAAGCTT
IRR : CGGAACGTGTCAACGACTGTGAGACAGCCGGAGTTATGAATTAGGCTTGG
Insertion site
| Left flank | Direct repeat | Right flank | DR Length |
|---|---|---|---|
| ATGGCGTGAG | CAATCTGATC | 0 | |
| GGAACTGGCG | GTT | CACCCGGTCG | 3 |
| AGTCTGCAGA | TTC | TGGCAGAAAG | 3 |
| GGCCGGCCAA | GAC | GACGCCCGGC | 3 |
DNA sequence
CCCTCTGTGCCAATGAGAGTGAGACACCGTGCCCCCTGAAGTTTAAGCTTGAGGGCTGAGGAAACTGATTTCGTGGTTATGCCCAACAATATGCCCCTGT
CCGCCCCTGTATCGGAGGCGGTTCCCGACGCCGGTGAGACGCCGCGGGTTGTTGCCGGGCCCGCTGCAGTCCCCCCGGAGATGTCGGCGCGTCCCAAACG
TCGCACTTTCACGGCGGCGGAGAAGCTGCGGATCCTCGCCGAGACGGATCGGGCGGCGGACACCGGCGGGATCGCCGCGATCCTGCGGCGGGAAGGTCTG
TATTCGTCGGCCCTGACCGACTGGCGCCGGCAACGCGATGCCGGCGCCTTCGAGGCGCTGAAGCCGCTCAAGCGCGGTCCGAAGACGGCCCCCTCCAACC
CGTTGGAAGCGGAGCTGGCCAAGGCTCGCCAGGACATCGCCCGTCTCCAGCGCCGCCTGGAACGAGCCGAGGCGGTCATCGACGTCCAAAAAAAATTTTC
GGACCTGTTGGGGATTGCCTTGCCTCCCGCCGACAGCGACGAGACGCCGCGATGAGCGCGATCGCCACCTTGGCCCCCGACCGCGGTCTTGTGAGTGCGG
CCTGCGCCGCTGTCGGCGTCGCCCGTGCCAGCGTCCATCGGCATCGGGTCCGGCTGGCCGGCCGGGAACCGGCGCCTCGCCCCCGGCCACGCCCGCCGCG
GGCGCTCGCGGTCCAGGAGCGCCAGACGGTGCTCGCCCTTCTGCGCGAGCCGCGCTTCGTCGATCTCGCCCCGGCGGAAGTCTACGCCACCCTGCTCGAT
GAAGGCGTCTATCATTGCTCCATCCGCACCATGTACCGCCTGCTGGCGGAGCATGACGAGGTCCGCGAACGCCGCGACCAGCTCCGTCATCCGGCCTACC
GGAAACCCGAATTGCTGGCCAAGGCCCCGAACGAGGTCTGGTCCTGGGACATCACCAAGCTGATGGGGCCGATGAAGTGGTACTACCTCTACGTGATCCT
CGACATCTTCAGCCGCCGCGTTGTCGGCTGGTGCGTCGCCGATGCCGAGAGCGCCACCCTGTTCAAGGCGCTGTTCGAGGAGACGCTGGCCAAACACACC
GTCCCGCCCGGCCAATTGACCCTGCACGCCGATCGAGGCGGCCCGATGAAGGCCAAGGCCACCGCTCTCCTGCTCGCCGACCTCGGCGTCACCAAATCGC
ACAGCCGGCCACACACCTCCAATGACAACCCGTTCTCGGAGGCCCACTTCAAGACCATGAAATATCAGCCGCAATTCCCCAAGCGCTTCGGCTCCATCGA
GGATGCCAGGGCCTTCTGCCGGGACTTCTTCACCTGGTACAACCGGGATCACCACCATGCCGGCATCGGCCTGATGACCCCAGACCAAGTCCATTACGGC
CAAGCCGACGCCGTCCATGCCGCCCGCCAGCAAACCCTCGACCGTGCCTTTCGCCGCACACCGGAGCGTTTCGTTCGCAAAGCGCCTCAACCACCCGCAA
AGCCAATCGAAGTCTGGATCAATCCGCCGCAAAAGCCGGAGAGCATCCAAGCCTAATTCATAACTCCGGCTGTCTCACAGTCGTTGACACGTTCCG
CCGCCCCTGTATCGGAGGCGGTTCCCGACGCCGGTGAGACGCCGCGGGTTGTTGCCGGGCCCGCTGCAGTCCCCCCGGAGATGTCGGCGCGTCCCAAACG
TCGCACTTTCACGGCGGCGGAGAAGCTGCGGATCCTCGCCGAGACGGATCGGGCGGCGGACACCGGCGGGATCGCCGCGATCCTGCGGCGGGAAGGTCTG
TATTCGTCGGCCCTGACCGACTGGCGCCGGCAACGCGATGCCGGCGCCTTCGAGGCGCTGAAGCCGCTCAAGCGCGGTCCGAAGACGGCCCCCTCCAACC
CGTTGGAAGCGGAGCTGGCCAAGGCTCGCCAGGACATCGCCCGTCTCCAGCGCCGCCTGGAACGAGCCGAGGCGGTCATCGACGTCCAAAAAAAATTTTC
GGACCTGTTGGGGATTGCCTTGCCTCCCGCCGACAGCGACGAGACGCCGCGATGAGCGCGATCGCCACCTTGGCCCCCGACCGCGGTCTTGTGAGTGCGG
CCTGCGCCGCTGTCGGCGTCGCCCGTGCCAGCGTCCATCGGCATCGGGTCCGGCTGGCCGGCCGGGAACCGGCGCCTCGCCCCCGGCCACGCCCGCCGCG
GGCGCTCGCGGTCCAGGAGCGCCAGACGGTGCTCGCCCTTCTGCGCGAGCCGCGCTTCGTCGATCTCGCCCCGGCGGAAGTCTACGCCACCCTGCTCGAT
GAAGGCGTCTATCATTGCTCCATCCGCACCATGTACCGCCTGCTGGCGGAGCATGACGAGGTCCGCGAACGCCGCGACCAGCTCCGTCATCCGGCCTACC
GGAAACCCGAATTGCTGGCCAAGGCCCCGAACGAGGTCTGGTCCTGGGACATCACCAAGCTGATGGGGCCGATGAAGTGGTACTACCTCTACGTGATCCT
CGACATCTTCAGCCGCCGCGTTGTCGGCTGGTGCGTCGCCGATGCCGAGAGCGCCACCCTGTTCAAGGCGCTGTTCGAGGAGACGCTGGCCAAACACACC
GTCCCGCCCGGCCAATTGACCCTGCACGCCGATCGAGGCGGCCCGATGAAGGCCAAGGCCACCGCTCTCCTGCTCGCCGACCTCGGCGTCACCAAATCGC
ACAGCCGGCCACACACCTCCAATGACAACCCGTTCTCGGAGGCCCACTTCAAGACCATGAAATATCAGCCGCAATTCCCCAAGCGCTTCGGCTCCATCGA
GGATGCCAGGGCCTTCTGCCGGGACTTCTTCACCTGGTACAACCGGGATCACCACCATGCCGGCATCGGCCTGATGACCCCAGACCAAGTCCATTACGGC
CAAGCCGACGCCGTCCATGCCGCCCGCCAGCAAACCCTCGACCGTGCCTTTCGCCGCACACCGGAGCGTTTCGTTCGCAAAGCGCCTCAACCACCCGCAA
AGCCAATCGAAGTCTGGATCAATCCGCCGCAAAAGCCGGAGAGCATCCAAGCCTAATTCATAACTCCGGCTGTCTCACAGTCGTTGACACGTTCCG
Recoding section
- Recoding by frameshift
- Frame
- Type
- Experimentally demonstrated
Stimulators :
- Shine-Dalgarno sequence :
- Secondary structure :
Recoding motif :
Protein section
ORF number : 3
ORF 1
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 483 bp | 160 aa | 73 | 555 | + | No |
Description : First part of the transposase
ORF sequence :
MVMPNNMPLSAPVSEAVPDAGETPRVVAGPAAVPPEMSARPKRRTFTAAEKLRILAETDRAADTGGIAAILRREGLYSSALTDWRRQRDAGAFEALKPLK
RGPKTAPSNPLEAELAKARQDIARLQRRLERAEAVIDVQKKFSDLLGIALPPADSDETPR
RGPKTAPSNPLEAELAKARQDIARLQRRLERAEAVIDVQKKFSDLLGIALPPADSDETPR
Blast result :ORF 2
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 1251 bp | 416 aa | 306 | 1556 | + | No |
Description : Second part of the transposase
ORF sequence :
VGPDRLAPATRCRRLRGAEAAQARSEDGPLQPVGSGAGQGSPGHRPSPAPPGTSRGGHRRPKKIFGPVGDCLASRRQRRDAAMSAIATLAPDRGLVSAAC
AAVGVARASVHRHRVRLAGREPAPRPRPRPPRALAVQERQTVLALLREPRFVDLAPAEVYATLLDEGVYHCSIRTMYRLLAEHDEVRERRDQLRHPAYRK
PELLAKAPNEVWSWDITKLMGPMKWYYLYVILDIFSRRVVGWCVADAESATLFKALFEETLAKHTVPPGQLTLHADRGGPMKAKATALLLADLGVTKSHS
RPHTSNDNPFSEAHFKTMKYQPQFPKRFGSIEDARAFCRDFFTWYNRDHHHAGIGLMTPDQVHYGQADAVHAARQQTLDRAFRRTPERFVRKAPQPPAKP
IEVWINPPQKPESIQA
AAVGVARASVHRHRVRLAGREPAPRPRPRPPRALAVQERQTVLALLREPRFVDLAPAEVYATLLDEGVYHCSIRTMYRLLAEHDEVRERRDQLRHPAYRK
PELLAKAPNEVWSWDITKLMGPMKWYYLYVILDIFSRRVVGWCVADAESATLFKALFEETLAKHTVPPGQLTLHADRGGPMKAKATALLLADLGVTKSHS
RPHTSNDNPFSEAHFKTMKYQPQFPKRFGSIEDARAFCRDFFTWYNRDHHHAGIGLMTPDQVHYGQADAVHAARQQTLDRAFRRTPERFVRKAPQPPAKP
IEVWINPPQKPESIQA
Blast result :ORF 3
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 1484 bp | 494 aa | 73 | 1556 | + | Yes |
Chemistry : DDE
ORF sequence :
MVMPNNMPLSAPVSEAVPDAGETPRVVAGPAAVPPEMSARPKRRTFTAAEKLRILAETDRAADTGGIAAILRREGLYSSALTDWRRQRDAGAFEALKPLK
RGPKTAPSNPLEAELAKARQDIARLQRRLERAEAVIDVQKKIFGPVGDCLASRRQRRDAAMSAIATLAPDRGLVSAACAAVGVARASVHRHRVRLAGREP
APRPRPRPPRALAVQERQTVLALLREPRFVDLAPAEVYATLLDEGVYHCSIRTMYRLLAEHDEVRERRDQLRHPAYRKPELLAKAPNEVWSWDITKLMGP
MKWYYLYVILDIFSRRVVGWCVADAESATLFKALFEETLAKHTVPPGQLTLHADRGGPMKAKATALLLADLGVTKSHSRPHTSNDNPFSEAHFKTMKYQP
QFPKRFGSIEDARAFCRDFFTWYNRDHHHAGIGLMTPDQVHYGQADAVHAARQQTLDRAFRRTPERFVRKAPQPPAKPIEVWINPPQKPESIQA
RGPKTAPSNPLEAELAKARQDIARLQRRLERAEAVIDVQKKIFGPVGDCLASRRQRRDAAMSAIATLAPDRGLVSAACAAVGVARASVHRHRVRLAGREP
APRPRPRPPRALAVQERQTVLALLREPRFVDLAPAEVYATLLDEGVYHCSIRTMYRLLAEHDEVRERRDQLRHPAYRKPELLAKAPNEVWSWDITKLMGP
MKWYYLYVILDIFSRRVVGWCVADAESATLFKALFEETLAKHTVPPGQLTLHADRGGPMKAKATALLLADLGVTKSHSRPHTSNDNPFSEAHFKTMKYQP
QFPKRFGSIEDARAFCRDFFTWYNRDHHHAGIGLMTPDQVHYGQADAVHAARQQTLDRAFRRTPERFVRKAPQPPAKPIEVWINPPQKPESIQA
Blast result :
Comments
ISAzs23 is 71% aa similar to ISGur10. The third ORF is a putative ORFAB transposase reconstructed in silico by possible -1 frameshift.
References
1] ISfinder annotation (2011)
2] Kaneko,T., Minamisawa,K., Isawa,T., Nakatsukasa,H., Mitsui,H., Kawaharada,Y., Nakamura,Y., Watanabe,A., Kawashima,K., Ono,A., Shimizu,Y., Takahashi,C., Minami,C., Fujishiro,T., Kohara,M., Katoh,M., Nakazaki,N., Nakayama,S., Yamada,M., Tabata,S. and Sato,S. (2010) DNA Res. 17 (1), 37-50.
2] Kaneko,T., Minamisawa,K., Isawa,T., Nakatsukasa,H., Mitsui,H., Kawaharada,Y., Nakamura,Y., Watanabe,A., Kawashima,K., Ono,A., Shimizu,Y., Takahashi,C., Minami,C., Fujishiro,T., Kohara,M., Katoh,M., Nakazaki,N., Nakayama,S., Yamada,M., Tabata,S. and Sato,S. (2010) DNA Res. 17 (1), 37-50.