ISAzs22
- Family ISAs1
- Group
Isoform Synonym(s)
| Accession number | Transposition | Origin | Host |
|---|---|---|---|
| NC_013856 | ND | Azospirillum sp. | Azospirillum sp. B510 plasmid pAB510b |
DNA section
IS Length : 1267 bp
Ends
IR Length : 18/20
IRL : CAGGGCGATTCAGAGAAGGTGGATGAAATTCGGGTGACGCGAGTCGTCGG
IRR : CAGGGCGATTCAAAGAAAGCCGTTTTTGACGGTTGCGATTGTGGCACAGC
Insertion site
| Left flank | Direct repeat | Right flank | DR Length |
|---|---|---|---|
| TCGCCGCCTG | GGGCGAAGCG | CGGCTCGACT | 10 |
| GATGACCCTG | GCCCTGGGTG | GCATACTTGA | 10 |
| GCTCCAGGCC | AAAGCGCGGG | 0 | |
| GACGCCACCT | ACCTCAAGCA | GCGCGAGGGG | 10 |
| CACGTCCGGC | GGCTTCCTGC | ACACCCCCCT | 10 |
DNA sequence
CAGGGCGATTCAGAGAAGGTGGATGAAATTCGGGTGACGCGAGTCGTCGGTTGATTCACAAGGCTATCGGCAGGAACCGAGCCGGAGAGTCGGAGCGATG
AGGGGTGGACCGCGGACGTTGTGGGAGGTTGTGGGAGCGATCCCGGATCGCCGGGGCCGGAAGGGGCGGCAGTACCCTCTGGCATCGGTTTTGATTTGGC
GCTTTCGGCGATGCTGGCGGGGGCCAACGACCTGATGGCGATCTTTCGCTGGGGGCGACGGTTGCCGCCCGAGGCTCTTTACCTGCTGGGCCTGGAGCGG
GCGCCGTGTCACGCCATGTACCACTATTTCTTCAAGGCTTTGGACGTTACGGCGGCCGAGGCGGTGTTGGGGGAATGGGCGCGCGGCTCCGGCCCGGTCG
GACATGTGGCGCTCGACGGCAAGCGCCTGAAGGGCAGCGCGCCGGCCGGGCACGATGGCAGCGAAGGCGTGCATCTGCTGGCCGCCTTCGCCAGCCGGCT
GGGGACGGTGATCGGCCAGTTGCGGGTGCCGCCCGAAGCCAACGAGATCACCGCCGCGCTGACGCTGCTCAAGAGCCTGCCGCTGGGCGGTGCGGTAATC
ACCGGGGATGCGGTGTTCTGTCAGCGCGCCATCTGCCAAGCCATCCGCGACGGCGGCGGCGATTATCTGTTCACCGTCAAAGCCAACCAACCGGCGCTGA
TGGCCGATCTCGCCGTCGCCTTCGGTGACGCTTTTCCCCCCGGAGCTGGTGGCGGCGTTCCAGGGCCAGCCCCCGCCTCCCGCCCTGGACGGGCCGCCGC
CGGACCGTGAGGGCGCGCAGACCGTGGAGAAGGGGCACGGGCGCATCGAAACCCGCCGCATCACCGTCAGCCGCGAGGTCGTTCCCGCGCTCGACTGGCC
CGGCGTGGCGCAGGTCTGCCGCATCGAGCGGACCCGCGAGGTCAAGGGCGCGATCTCGCACGAAACCGTCTACGTCATCACCAGCCTGGACCGCGACCAC
GCCTCGCCTGCGGCGCTGCTCGCGCTCGCCCGCGACCACTGGGGCATCGAGAACCGCCTGCACTGGAGGCGTGACGTCCTCTTGAGCGAAGACGCCAGCC
GGGTGCGATCCGGCGCCGTGCCACAGGCCATGGCGATGCTGCGCAACACCATGCTGGGCATCGCCAATCTCTTCGGCGCCCCACTCCCCGCCGTCCGCAT
GGCATGCGCCGAAGATCGCTGTGCCACAATCGCAACCGTCAAAAACGGCTTTCTTTGAATCGCCCTG
AGGGGTGGACCGCGGACGTTGTGGGAGGTTGTGGGAGCGATCCCGGATCGCCGGGGCCGGAAGGGGCGGCAGTACCCTCTGGCATCGGTTTTGATTTGGC
GCTTTCGGCGATGCTGGCGGGGGCCAACGACCTGATGGCGATCTTTCGCTGGGGGCGACGGTTGCCGCCCGAGGCTCTTTACCTGCTGGGCCTGGAGCGG
GCGCCGTGTCACGCCATGTACCACTATTTCTTCAAGGCTTTGGACGTTACGGCGGCCGAGGCGGTGTTGGGGGAATGGGCGCGCGGCTCCGGCCCGGTCG
GACATGTGGCGCTCGACGGCAAGCGCCTGAAGGGCAGCGCGCCGGCCGGGCACGATGGCAGCGAAGGCGTGCATCTGCTGGCCGCCTTCGCCAGCCGGCT
GGGGACGGTGATCGGCCAGTTGCGGGTGCCGCCCGAAGCCAACGAGATCACCGCCGCGCTGACGCTGCTCAAGAGCCTGCCGCTGGGCGGTGCGGTAATC
ACCGGGGATGCGGTGTTCTGTCAGCGCGCCATCTGCCAAGCCATCCGCGACGGCGGCGGCGATTATCTGTTCACCGTCAAAGCCAACCAACCGGCGCTGA
TGGCCGATCTCGCCGTCGCCTTCGGTGACGCTTTTCCCCCCGGAGCTGGTGGCGGCGTTCCAGGGCCAGCCCCCGCCTCCCGCCCTGGACGGGCCGCCGC
CGGACCGTGAGGGCGCGCAGACCGTGGAGAAGGGGCACGGGCGCATCGAAACCCGCCGCATCACCGTCAGCCGCGAGGTCGTTCCCGCGCTCGACTGGCC
CGGCGTGGCGCAGGTCTGCCGCATCGAGCGGACCCGCGAGGTCAAGGGCGCGATCTCGCACGAAACCGTCTACGTCATCACCAGCCTGGACCGCGACCAC
GCCTCGCCTGCGGCGCTGCTCGCGCTCGCCCGCGACCACTGGGGCATCGAGAACCGCCTGCACTGGAGGCGTGACGTCCTCTTGAGCGAAGACGCCAGCC
GGGTGCGATCCGGCGCCGTGCCACAGGCCATGGCGATGCTGCGCAACACCATGCTGGGCATCGCCAATCTCTTCGGCGCCCCACTCCCCGCCGTCCGCAT
GGCATGCGCCGAAGATCGCTGTGCCACAATCGCAACCGTCAAAAACGGCTTTCTTTGAATCGCCCTG
Recoding section
- Recoding by frameshift
- Frame
- Type
- Experimentally demonstrated
Stimulators :
- Shine-Dalgarno sequence :
- Secondary structure :
Recoding motif :
Protein section
ORF number : 3
ORF 1
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 690 bp | 229 aa | 121 | 810 | + | No |
Description : First part of the transposase
ORF sequence :
MGGCGSDPGSPGPEGAAVPSGIGFDLALSAMLAGANDLMAIFRWGRRLPPEALYLLGLERAPCHAMYHYFFKALDVTAAEAVLGEWARGSGPVGHVALDG
KRLKGSAPAGHDGSEGVHLLAAFASRLGTVIGQLRVPPEANEITAALTLLKSLPLGGAVITGDAVFCQRAICQAIRDGGGDYLFTVKANQPALMADLAVA
FGDAFPPGAGGGVPGPAPASRPGRAAAGP
KRLKGSAPAGHDGSEGVHLLAAFASRLGTVIGQLRVPPEANEITAALTLLKSLPLGGAVITGDAVFCQRAICQAIRDGGGDYLFTVKANQPALMADLAVA
FGDAFPPGAGGGVPGPAPASRPGRAAAGP
Blast result :ORF 2
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 557 bp | 185 aa | 702 | 1258 | + | No |
Description : Second part of the transposase
ORF sequence :
WPISPSPSVTLFPPELVAAFQGQPPPPALDGPPPDREGAQTVEKGHGRIETRRITVSREVVPALDWPGVAQVCRIERTREVKGAISHETVYVITSLDRDH
ASPAALLALARDHWGIENRLHWRRDVLLSEDASRVRSGAVPQAMAMLRNTMLGIANLFGAPLPAVRMACAEDRCATIATVKNGFL
ASPAALLALARDHWGIENRLHWRRDVLLSEDASRVRSGAVPQAMAMLRNTMLGIANLFGAPLPAVRMACAEDRCATIATVKNGFL
Blast result :ORF 3
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 1138 bp | 378 aa | 121 | 1258 | + | Yes |
Chemistry : Unknow
ORF sequence :
MGGCGSDPGSPGPEGAAVPSGIGFDLALSAMLAGANDLMAIFRWGRRLPPEALYLLGLERAPCHAMYHYFFKALDVTAAEAVLGEWARGSGPVGHVALDG
KRLKGSAPAGHDGSEGVHLLAAFASRLGTVIGQLRVPPEANEITAALTLLKSLPLGGAVITGDAVFCQRAICQAIRDGGGDYLFTVKANQPALMADLAVA
FGDAFPPELVAAFQGQPPPPALDGPPPDREGAQTVEKGHGRIETRRITVSREVVPALDWPGVAQVCRIERTREVKGAISHETVYVITSLDRDHASPAALL
ALARDHWGIENRLHWRRDVLLSEDASRVRSGAVPQAMAMLRNTMLGIANLFGAPLPAVRMACAEDRCATIATVKNGFL
KRLKGSAPAGHDGSEGVHLLAAFASRLGTVIGQLRVPPEANEITAALTLLKSLPLGGAVITGDAVFCQRAICQAIRDGGGDYLFTVKANQPALMADLAVA
FGDAFPPELVAAFQGQPPPPALDGPPPDREGAQTVEKGHGRIETRRITVSREVVPALDWPGVAQVCRIERTREVKGAISHETVYVITSLDRDHASPAALL
ALARDHWGIENRLHWRRDVLLSEDASRVRSGAVPQAMAMLRNTMLGIANLFGAPLPAVRMACAEDRCATIATVKNGFL
Blast result :
Comments
ISAzs22 is 48% aa similar to ISTha1. The third ORF is a possible ORFAB transposase reconstructed in silico by possible +1 frameshift.
References
1] ISfinder annotation (2011)
2] Kaneko,T., Minamisawa,K., Isawa,T., Nakatsukasa,H., Mitsui,H., Kawaharada,Y., Nakamura,Y., Watanabe,A., Kawashima,K., Ono,A., Shimizu,Y., Takahashi,C., Minami,C., Fujishiro,T., Kohara,M., Katoh,M., Nakazaki,N., Nakayama,S., Yamada,M., Tabata,S. and Sato,S. (2010) DNA Res. 17 (1), 37-50.
2] Kaneko,T., Minamisawa,K., Isawa,T., Nakatsukasa,H., Mitsui,H., Kawaharada,Y., Nakamura,Y., Watanabe,A., Kawashima,K., Ono,A., Shimizu,Y., Takahashi,C., Minami,C., Fujishiro,T., Kohara,M., Katoh,M., Nakazaki,N., Nakayama,S., Yamada,M., Tabata,S. and Sato,S. (2010) DNA Res. 17 (1), 37-50.