ISAzs21
- Family IS66
- Group
Isoform Synonym(s)
| Accession number | Transposition | Origin | Host |
|---|---|---|---|
| NC_013855 | ND | Azospirillum sp. | Azospirillum sp. B510 plasmid pAB510f Azospirillum sp. B510 plasmid pAB510a |
DNA section
IS Length : 2598 bp
Ends
IR Length : 13/15
IRL : GTAACCGTCCGACGATGGCCGCCTTGCGGGGAGTGGACGAAGCGGGCTGA
IRR : GTAAGCGTCCGCCGAGCTACGCGGCGTCGGCGTTTTCGGTAACGTCGGCG
Insertion site
| Left flank | Direct repeat | Right flank | DR Length |
|---|---|---|---|
| AGGAGACCCT | GAATGGCC | CGACACAAAG | 8 |
DNA sequence
GTAACCGTCCGACGATGGCCGCCTTGCGGGGAGTGGACGAAGCGGGCTGAGTGAGGACGCCGCGGATGGCCGCGGCTTGGTCCTATGTCCAGCCCCATGG
CTATGCTTATGACTTACCAGCGCGTCGAGGTGATCACGGGATCGGAGAAGCGCCGCTTTTATAGCGCCGCGGACAAGGCGGGCTTGGTGGCGGAAGCGTT
CCGGCCCGGCGTGGTGGCGTCCGAGGTGGCTCGTCGGCACGGCCTCAACGTCAGCCTGCTGTACCGCTGGCGCCGGCAGATCGAGGAGGAGCAGGCGAGC
CCGTCGCCCGAACCAGCGGTGAACTTCGTGCCCGTGCGGATCGCCGACGCCCCGCCGTCCTTCCCGGACGACAAGATGGCGCCCCTTCCGCCCGCCGGAA
CCGGCATCATCGAAATCGTGCTGCCCAGCGGCCATTGCCTGCGCGTCGATCGCCATGTCGATGCGGGCGCCCTGCGTCGGGTCCTCGCCGTTCTGGAGCG
CCGATGATCCCGGTCCCCGCCGGCGTGCGGATCTACCTGGCGATGGGCGCCACCGACATGCGCAAGGGCTTCGACGGCCTCGCCCTGTTGGTCCAGGAGG
TTCTCAAGAAGGATCCCTACTCCGGCCATCTCTTCCTGTTCCGGGGCCGCCGAGCCGACAAGATCAAGATTCTCTACTACGACACCAACGGCCTGTGCCT
GTTCGCCAAGCGCCTTCAGCGCGGCCGGTTCGTCTGGCCGGTGTCGCGCGAGGGCGTGGTGACGCTGACCCCGGCGGAGCTGGCGATGCTCGTGGAAGGG
CTGGACTGGCGCGCGGCGGAACGGACCTGGAAGCCCGACTTGGCCGGCTGACCCGGCGCGGGCCGGGCGCGACACGGTGACTCAGCCGCCCTCGGGCCGG
TCGTCGGGATCCCGGTGTCCTGGTAAAATCGCGCCATGGTTTCCGATCCCGCCCAGCTTCTGGCCGACAACGCGGCGTTGCGGCAGCTCGTCGCCGAGCA
GGCGGCCCGGCTGCATGCCCAGGAGGCGGCGTTGCGCGCCAAGGACGCGGCGCTCGGCGCGGCGCTGGAGGGCATCAAGAGCAAGGCGCTGGAGATCGAG
AAGCTGAAGCTTCAGCTCGCCCGGCTGCGCCGCATGCACTTCGGCCAGTCCTCGGAAAAGCTGGGCCGAACCATCGCCCAGCTGGAACTGGCGCTGGAAG
ACCTGGAAGCCGCCGAAGGCGAGCAGGCCGCCATCGTCACGCAGCCCGACGCGCCGCCAGAAGACGCCGAAACTAGGCGCCGGCCCAAGCGCACGCTGCC
GCCCGAGCATCTGCCGCGCGAGGAGCAGGTGCACGAGCCCGAAGGCGGCACCTGCACCTGCGCGCAGTGCGGTGGCGCGCTGCGTCGGCTGGGCGAGGAC
CGCGCCCGGCAGCTGGAATACGTGCCGGCGCACTTCAAGGTGGTCGAGCACGTCCGCCCGAAGTTCTCGTGCCGGCGCTGCGAGGCGATCACGCAGGCAC
CAGCGTCGCTGCCGATCGAGCGGGGCCTGTTCGGGCCGGGGCTGTTGGCGCACCTGCTGGTTAGCAAGTTCGACGATCATCTGCCGCTGTATCGCCAGGC
GGAGATCTTCGCCCGCCTGGGCCTGGAGTTGAGCCGCTCCACCCTGGCCGATCAGGTCGGCCACGCGGCTCGGCTGCTGCGCCCGCTGATCCAGGCGATC
GAAACCAGCGTCATCGGTTCCGCCAAGCTGCACGCCGACGACACGCCGGTCCCGGTGCTGAGCCCGGGGGCCGGGCGGACCAAGACCGGCTACCTGTGGG
TCTACCTGCGCGATGATCGCCCCTGGAACGGCCCGTCGCCTCCGGCGGCGCTGTACCGCTACAGCCCCGGGCGCGGCGGCGAGCACCCGTGCGGCCACCT
GAAGGATTTCCACGGGGCGCTGCAGGCGGACGGCTATGCCGGGTTCAACGGCCTGTACGAACCGGGCCCCGACGGGCGGGCCCGGATCATTGAAATTGGC
TGCATGGCCCATGGCCGTCGCAAGTTCAACGACGTGGCGGTCTCGGCCAAGGGCAAGGCGCCGATCGCGGCCGAGGCACTGCGGCGGATCGGCGAGCTGT
ATGCGGTCGAAGCCCAGATCGCCGGCCGGGATGCCGACGAGCGCCGGCGCGTGCGCCAGGCCGAGGCTGCCCCGAAAATGGCCGCGTTGAAGGCATGGCT
GGAGGAGACGCTGGGCCGGCTCGACGGCAAGTCGGACACGGCCAAGGCGGTGCGCTACCTGCTCAACCGCTGGACGGCGCTGACCCGTTACCTGGACGAT
GGCCGTCTGGAGATCGACAACAACGCCGCCGAAAGGGCCTTGCGGGCCGTCGTGGTGGGCCGCAAGAACTGGCTGTTCGCCGGAGCCGATGTCGGGGGTG
AGCGGGCCGCGGCGATGTACACGCTCATCGAGACGGCCAAGCTCAACGGGCTCGACCCCGAGGCGTACCTGCGCGACGTGATCGCCCGGATTGCCGATCA
CAAGATCAGCCGCATCGACGAGTTGCTGCCCTGGAACTGGACCAAAGCCGCCGACGTTACCGAAAACGCCGACGCCGCGTAGCTCGGCGGACGCTTAC
CTATGCTTATGACTTACCAGCGCGTCGAGGTGATCACGGGATCGGAGAAGCGCCGCTTTTATAGCGCCGCGGACAAGGCGGGCTTGGTGGCGGAAGCGTT
CCGGCCCGGCGTGGTGGCGTCCGAGGTGGCTCGTCGGCACGGCCTCAACGTCAGCCTGCTGTACCGCTGGCGCCGGCAGATCGAGGAGGAGCAGGCGAGC
CCGTCGCCCGAACCAGCGGTGAACTTCGTGCCCGTGCGGATCGCCGACGCCCCGCCGTCCTTCCCGGACGACAAGATGGCGCCCCTTCCGCCCGCCGGAA
CCGGCATCATCGAAATCGTGCTGCCCAGCGGCCATTGCCTGCGCGTCGATCGCCATGTCGATGCGGGCGCCCTGCGTCGGGTCCTCGCCGTTCTGGAGCG
CCGATGATCCCGGTCCCCGCCGGCGTGCGGATCTACCTGGCGATGGGCGCCACCGACATGCGCAAGGGCTTCGACGGCCTCGCCCTGTTGGTCCAGGAGG
TTCTCAAGAAGGATCCCTACTCCGGCCATCTCTTCCTGTTCCGGGGCCGCCGAGCCGACAAGATCAAGATTCTCTACTACGACACCAACGGCCTGTGCCT
GTTCGCCAAGCGCCTTCAGCGCGGCCGGTTCGTCTGGCCGGTGTCGCGCGAGGGCGTGGTGACGCTGACCCCGGCGGAGCTGGCGATGCTCGTGGAAGGG
CTGGACTGGCGCGCGGCGGAACGGACCTGGAAGCCCGACTTGGCCGGCTGACCCGGCGCGGGCCGGGCGCGACACGGTGACTCAGCCGCCCTCGGGCCGG
TCGTCGGGATCCCGGTGTCCTGGTAAAATCGCGCCATGGTTTCCGATCCCGCCCAGCTTCTGGCCGACAACGCGGCGTTGCGGCAGCTCGTCGCCGAGCA
GGCGGCCCGGCTGCATGCCCAGGAGGCGGCGTTGCGCGCCAAGGACGCGGCGCTCGGCGCGGCGCTGGAGGGCATCAAGAGCAAGGCGCTGGAGATCGAG
AAGCTGAAGCTTCAGCTCGCCCGGCTGCGCCGCATGCACTTCGGCCAGTCCTCGGAAAAGCTGGGCCGAACCATCGCCCAGCTGGAACTGGCGCTGGAAG
ACCTGGAAGCCGCCGAAGGCGAGCAGGCCGCCATCGTCACGCAGCCCGACGCGCCGCCAGAAGACGCCGAAACTAGGCGCCGGCCCAAGCGCACGCTGCC
GCCCGAGCATCTGCCGCGCGAGGAGCAGGTGCACGAGCCCGAAGGCGGCACCTGCACCTGCGCGCAGTGCGGTGGCGCGCTGCGTCGGCTGGGCGAGGAC
CGCGCCCGGCAGCTGGAATACGTGCCGGCGCACTTCAAGGTGGTCGAGCACGTCCGCCCGAAGTTCTCGTGCCGGCGCTGCGAGGCGATCACGCAGGCAC
CAGCGTCGCTGCCGATCGAGCGGGGCCTGTTCGGGCCGGGGCTGTTGGCGCACCTGCTGGTTAGCAAGTTCGACGATCATCTGCCGCTGTATCGCCAGGC
GGAGATCTTCGCCCGCCTGGGCCTGGAGTTGAGCCGCTCCACCCTGGCCGATCAGGTCGGCCACGCGGCTCGGCTGCTGCGCCCGCTGATCCAGGCGATC
GAAACCAGCGTCATCGGTTCCGCCAAGCTGCACGCCGACGACACGCCGGTCCCGGTGCTGAGCCCGGGGGCCGGGCGGACCAAGACCGGCTACCTGTGGG
TCTACCTGCGCGATGATCGCCCCTGGAACGGCCCGTCGCCTCCGGCGGCGCTGTACCGCTACAGCCCCGGGCGCGGCGGCGAGCACCCGTGCGGCCACCT
GAAGGATTTCCACGGGGCGCTGCAGGCGGACGGCTATGCCGGGTTCAACGGCCTGTACGAACCGGGCCCCGACGGGCGGGCCCGGATCATTGAAATTGGC
TGCATGGCCCATGGCCGTCGCAAGTTCAACGACGTGGCGGTCTCGGCCAAGGGCAAGGCGCCGATCGCGGCCGAGGCACTGCGGCGGATCGGCGAGCTGT
ATGCGGTCGAAGCCCAGATCGCCGGCCGGGATGCCGACGAGCGCCGGCGCGTGCGCCAGGCCGAGGCTGCCCCGAAAATGGCCGCGTTGAAGGCATGGCT
GGAGGAGACGCTGGGCCGGCTCGACGGCAAGTCGGACACGGCCAAGGCGGTGCGCTACCTGCTCAACCGCTGGACGGCGCTGACCCGTTACCTGGACGAT
GGCCGTCTGGAGATCGACAACAACGCCGCCGAAAGGGCCTTGCGGGCCGTCGTGGTGGGCCGCAAGAACTGGCTGTTCGCCGGAGCCGATGTCGGGGGTG
AGCGGGCCGCGGCGATGTACACGCTCATCGAGACGGCCAAGCTCAACGGGCTCGACCCCGAGGCGTACCTGCGCGACGTGATCGCCCGGATTGCCGATCA
CAAGATCAGCCGCATCGACGAGTTGCTGCCCTGGAACTGGACCAAAGCCGCCGACGTTACCGAAAACGCCGACGCCGCGTAGCTCGGCGGACGCTTAC
Protein section
ORF number : 3
ORF 1
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 423 bp | 140 aa | 85 | 507 | + | No |
AG :
ORF sequence :
MSSPMAMLMTYQRVEVITGSEKRRFYSAADKAGLVAEAFRPGVVASEVARRHGLNVSLLYRWRRQIEEEQASPSPEPAVNFVPVRIADAPPSFPDDKMAP
LPPAGTGIIEIVLPSGHCLRVDRHVDAGALRRVLAVLERR
LPPAGTGIIEIVLPSGHCLRVDRHVDAGALRRVLAVLERR
Blast result :ORF 2
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 348 bp | 115 aa | 504 | 851 | + | No |
AG : IS66 TnpB
ORF sequence :
MIPVPAGVRIYLAMGATDMRKGFDGLALLVQEVLKKDPYSGHLFLFRGRRADKIKILYYDTNGLCLFAKRLQRGRFVWPVSREGVVTLTPAELAMLVEGL
DWRAAERTWKPDLAG
DWRAAERTWKPDLAG
Blast result :ORF 3
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 1647 bp | 548 aa | 936 | 2582 | + | No |
Chemistry : DDE
ORF sequence :
MVSDPAQLLADNAALRQLVAEQAARLHAQEAALRAKDAALGAALEGIKSKALEIEKLKLQLARLRRMHFGQSSEKLGRTIAQLELALEDLEAAEGEQAAI
VTQPDAPPEDAETRRRPKRTLPPEHLPREEQVHEPEGGTCTCAQCGGALRRLGEDRARQLEYVPAHFKVVEHVRPKFSCRRCEAITQAPASLPIERGLFG
PGLLAHLLVSKFDDHLPLYRQAEIFARLGLELSRSTLADQVGHAARLLRPLIQAIETSVIGSAKLHADDTPVPVLSPGAGRTKTGYLWVYLRDDRPWNGP
SPPAALYRYSPGRGGEHPCGHLKDFHGALQADGYAGFNGLYEPGPDGRARIIEIGCMAHGRRKFNDVAVSAKGKAPIAAEALRRIGELYAVEAQIAGRDA
DERRRVRQAEAAPKMAALKAWLEETLGRLDGKSDTAKAVRYLLNRWTALTRYLDDGRLEIDNNAAERALRAVVVGRKNWLFAGADVGGERAAAMYTLIET
AKLNGLDPEAYLRDVIARIADHKISRIDELLPWNWTKAADVTENADAA
VTQPDAPPEDAETRRRPKRTLPPEHLPREEQVHEPEGGTCTCAQCGGALRRLGEDRARQLEYVPAHFKVVEHVRPKFSCRRCEAITQAPASLPIERGLFG
PGLLAHLLVSKFDDHLPLYRQAEIFARLGLELSRSTLADQVGHAARLLRPLIQAIETSVIGSAKLHADDTPVPVLSPGAGRTKTGYLWVYLRDDRPWNGP
SPPAALYRYSPGRGGEHPCGHLKDFHGALQADGYAGFNGLYEPGPDGRARIIEIGCMAHGRRKFNDVAVSAKGKAPIAAEALRRIGELYAVEAQIAGRDA
DERRRVRQAEAAPKMAALKAWLEETLGRLDGKSDTAKAVRYLLNRWTALTRYLDDGRLEIDNNAAERALRAVVVGRKNWLFAGADVGGERAAAMYTLIET
AKLNGLDPEAYLRDVIARIADHKISRIDELLPWNWTKAADVTENADAA
Blast result :
Comments
ISAzs21 is 72% (ORFC : the transposase) aa similar to ISAzs18.
References
1] ISfinder annotation (2011)
2] Kaneko,T., Minamisawa,K., Isawa,T., Nakatsukasa,H., Mitsui,H., Kawaharada,Y., Nakamura,Y., Watanabe,A., Kawashima,K., Ono,A., Shimizu,Y., Takahashi,C., Minami,C., Fujishiro,T., Kohara,M., Katoh,M., Nakazaki,N., Nakayama,S., Yamada,M., Tabata,S. and Sato,S. (2010) DNA Res. 17 (1), 37-50.
2] Kaneko,T., Minamisawa,K., Isawa,T., Nakatsukasa,H., Mitsui,H., Kawaharada,Y., Nakamura,Y., Watanabe,A., Kawashima,K., Ono,A., Shimizu,Y., Takahashi,C., Minami,C., Fujishiro,T., Kohara,M., Katoh,M., Nakazaki,N., Nakayama,S., Yamada,M., Tabata,S. and Sato,S. (2010) DNA Res. 17 (1), 37-50.