ISfinder Mirror Site

ISAzs20

Family IS66
Group

MGE type ISRelated element(s) :
Isoform Synonym(s)

Accession number	Transposition	Origin	Host
NC_013854	ND	Azospirillum sp.	Azospirillum sp. B510 plasmid pAB510b Azospirillum sp. B510 plasmid pAB510f Azospirillum sp. B510 Azospirillum sp. B510 plasmid pAB510c

DNA section

IS Length : 2811 bp

Ends

IR Length : 16/22

IRL : GTACCCCTCCGCCGAGCGCCGCCTTGTGACCGAGCGGGGAAGCTGAATCA
IRR : GTACGCATCCGGTGAGTGACGCGGGTGCGGATCGAATCGTTCAGCGTGCT

Insertion site

Left flank	Direct repeat	Right flank	DR Length
ACGCTATAGC		GCCTAATTCA	0
CCCGGAGCCG	ACAAGGTG	CGCCGCTTCA	8
TCGGGTGATC		GCTCTATCGG	0
CGCGTGCTTC		CCACCACCAG	0

DNA sequence

GTACCCCTCCGCCGAGCGCCGCCTTGTGACCGAGCGGGGAAGCTGAATCATTGTGCCTGTCCCTATGGCTATCCCTATGGCAGTCCCTATGACTATCCAA
CGCGTCGAGGTGATCACGGGCCAGGAGCGGCGGCGGCAGTTCAGCGACGAGGAGAAGCTGCGGCTGGTCGAAGAGGCGTTCCAGCCGGGCGTCAAGGCGA
CCGAAGTCGCCCGGCGCCTGGGCGTGGACGTCAGCCTGCTGTACCGCTGGCGCCGCCAGTTCTTCGGTCAGCAGCCCCGGCTGCCCGCCTTCATGCCGAT
CACCGTCGCCACCGACGCTCCGGCACCGGAGGAGGTGGCAGAGCCGACAGCGGCGCCAGCGGCCCCACCAGCCGGTCTCATCGAGGTCGAATTCGCGACG
GCGCGCTTGCGCATCACCGGCCCGGTCGATCCGGCCCTGGTCGGCACGGTGATCGCCGCGCTGTCGGGACGGTCGGCATGATCCCGGTCCCCTCGGGCGT
TCGGGTCTGGCTGGCGGGCGGGGTCACCGACATGCGCTGCGGGATGAACTCTCTGGCGCTGAAGGTCCAGGAGGGTCTTGGCCGCGATCCCCATGCCGGC
GATTTATACGTCTTCCGTGGACGTCGCGGTGACATGGTGAAGTGCCTGTGGCATGACGGGCTCGGCATGTCGCTGTACGCCAAGAGGCTTGAACGGGGCC
GTTTCATCTGGCCCAGCCCGGCCAGCGGAGCCGTGGCCATTTCCGCGTCCCAGTTCGCGTATCTGCTCGACGCCATCGACTGGCGCAATCCGCAGCAGAC
TTGGAGACCGCGCTCGGCCGGATAGGCTGCGGCACAGTGAATCAACCCGCCGCTCTCCGGCGGGAAGAGCGAGGATCGGTGCGGGTTTCCGGGTACAATC
CGGCCCCATGGACGCCCTGCCCGACACCATCGACGCCCTGCGCGCCGCGCTGATCGAAGCGCGTGGCCGGGCTGCGTTGGCCGAGGCGGACGCCGCCCAG
GCGCGGGCCGAACGGTCCGGCGACCAGGCGCTGATCGCCACCCTGAAGCTCCAGATCGAGAAGCTCCAGCGCGACCTCTACGGCCGGCGCTCCGAACGGA
CCTCCCGACTGCTCGGCCAGCTCGAATTCCAGTTGGAGGAGGCGCAGGCAAGCGTCGGCGAGGACGATCTGGCGGCGGAACAGGTGGCCGAGGCGACCGG
CGCGGCCCGCATCACCCGCAAGGCGCCGTCGCGCAAGCCGTTCCCGGCGCACCTGCCGCGCGAGCGCGTCGTGATCCCGGCGCCGGCGGTGTGCCCATGC
TGCGGCTCGACCCGGCTGTGCAAGCTGGGTGAAAGCGTGACCGAGACGGCCGAGCGCATTCCCGCGCGGTGGAAAATCATCCAGACGGTGCGCGAGAAAT
TCTCCTGCCGGGACTGCGAGACGATCAGCCAGCCGCCGGCACCGTTCCACACCACGCCGCGCGGCTGGGCGGGGCCGAACCTGCTGGCCACGCTGCTGTT
CGAGAAGTTCGGCCAGCATCAGCCGCTGAACCGCCAGTGCGAGCGCTTCGCCAAGGAGGGCATGGAGATCAGCCTGTCCACCGCGGCCGACCAGGTGGGG
GCGGCCTGCGGCGTGCTGAAGCCGCTGCTCGACCGGCTCGCAGCGCATGTGCTGGCGGCGGAGCGGTTGCACGGCGAGCCCGAAGGGCCAGCGAAGCTAC
GACACGACCGTGCCGGTGCTGGCGAAGGGCAAGACCGACACCGGCCGCATCTGGGTCTATGTGCGCGACGATCGCCCCTTCGCGGGCGCGGCGGCACCGG
CGGCGCTGTTCCACTACTCCCGCGACCGTGGCGGCGGGCATCCCGAGGCACATCTGGCCGGTTGGGCCGGAGTGCTGCAGGCCGACGCCTATGCCGGATA
CAACCGCCTCTACGACGCGAGCCGCCAGCCGGAGCCCGTGGCCGAGGTCCTGTGTTGGGCGCACGCGCGTCGGAAATTCTTCGAACTCGCCGATATCGCC
GCCAACAAGCGGCGCGGCAAGGGGGCGCCCCCGATTTCTCCGCTGGCGCTGGAGGCGGTGCGGCGCATCGACCCGCTGTTCGACATCGAGCGCGAAGCCC
TCGGGCGCTCGGCAGCCGACCGTCTGGCGGTGCGCACAGAGCTGTCCAAGCCCCAGGTCGAGGAACTGGAAAACTGGATGCGAACGGCCCGGGCCGGGAT
GTCGAAGCACGCGCCGGTGGCCAAGGCGATGGACTACATGCTGACCCGCTGGGAAGGCTTCACCCGCTTCCTGCGTGACGGACGGGTCTGCCTGACCAAC
AATGCTGCCGAACGAGCGTTGCGTGGAATCGCCCTTGGCAGGAAGGCATGGCTATTCTGCGGATCGGATCGAGGCGGGCAGCGCGCCGCCGCCATGTACA
GCCTGATCGTGACGGCAAAGATGAACGACATCGATCCCCAGGCGTGGCTGGCCGACGTCCTGGCCCGCATCAACGATCTGCCGCAGACCAAGCTGCACGA
ACTGCTGCCCTGGGAATGGAAGCGGCTGCACGAGGCGACCACGGCGACCTGAGGAACCATGGCCAGAACCCGCAACCTCGTGACCATGGAGCGCGTCGCC
GAGATCCTCGGCGAGGACGTCGAATGGCTGATCGACATTGCCATCGAGCTGGAGCCGGAGGACGGTTGCCTGGCCGTGTTCGGCCCAGGCGAGCAGTGGT
TCCATGCCCTGACCGAAGATGGCGTCGAAAGCCTTAAGGAACTCATCCAAATCCACCGAGCAGCACGCTGAACGATTCGATCCGCACCCGCGTCACTCAC
CGGATGCGTAC

Recoding section

Recoding by frameshift
Frame
Type
Experimentally demonstrated

Stimulators :

Shine-Dalgarno sequence :
Secondary structure :

Recoding motif :

Protein section

ORF number : 3

ORF 1

Length		Begin	End	Strand	Fusion ORF
393 bp	130 aa	89	481	+	No

ORF function : Accessory Gene
AG : IS66 TnpA

ORF sequence :

MTIQRVEVITGQERRRQFSDEEKLRLVEEAFQPGVKATEVARRLGVDVSLLYRWRRQFFGQQPRLPAFMPITVATDAPAPEEVAEPTAAPAAPPAGLIEV
EFATARLRITGPVDPALVGTVIAALSGRSA

Blast result :

ORF 2

Length		Begin	End	Strand	Fusion ORF
348 bp	115 aa	478	825	+	No

ORF function : Accessory Gene
AG : IS66 TnpB

ORF sequence :

MIPVPSGVRVWLAGGVTDMRCGMNSLALKVQEGLGRDPHAGDLYVFRGRRGDMVKCLWHDGLGMSLYAKRLERGRFIWPSPASGAVAISASQFAYLLDAI
DWRNPQQTWRPRSAG

Blast result :

ORF 3

Length		Begin	End	Strand	Fusion ORF
1645 bp	548 aa	908	2552	+	Yes

ORF function : Transposase
Chemistry : DDE

ORF sequence :

MDALPDTIDALRAALIEARGRAALAEADAAQARAERSGDQALIATLKLQIEKLQRDLYGRRSERTSRLLGQLEFQLEEAQASVGEDDLAAEQVAEATGAA
RITRKAPSRKPFPAHLPRERVVIPAPAVCPCCGSTRLCKLGESVTETAERIPARWKIIQTVREKFSCRDCETISQPPAPFHTTPRGWAGPNLLATLLFEK
FGQHQPLNRQCERFAKEGMEISLSTAADQVGAACGVLKPLLDRLAAHVLAAERLHGEPPKGQRSYDTTVPVLAKGKTDTGRIWVYVRDDRPFAGAAAPAA
LFHYSRDRGGGHPEAHLAGWAGVLQADAYAGYNRLYDASRQPEPVAEVLCWAHARRKFFELADIAANKRRGKGAPPISPLALEAVRRIDPLFDIEREALG
RSAADRLAVRTELSKPQVEELENWMRTARAGMSKHAPVAKAMDYMLTRWEGFTRFLRDGRVCLTNNAAERALRGIALGRKAWLFCGSDRGGQRAAAMYSL
IVTAKMNDIDPQAWLADVLARINDLPQTKLHELLPWEWKRLHEATTAT

Blast result :

Comments

ISAzs20 is 84% (ORFC : the transposase) aa similar to ISAli10. The transposase is reconstructed in silico, there is a non programmed frameshift inside.

References

1] ISfinder annotation (2010).
2] Kaneko,T., Minamisawa,K., Isawa,T., Nakatsukasa,H., Mitsui,H., Kawaharada,Y., Nakamura,Y., Watanabe,A., Kawashima,K., Ono,A., Shimizu,Y., Takahashi,C., Minami,C., Fujishiro,T., Kohara,M., Katoh,M., Nakazaki,N., Nakayama,S., Yamada,M., Tabata,S. and Sato,S. (2010) DNA Res. 17 (1), 37-50.