ISAzs19
- Family IS256
- Group
Isoform Synonym(s)
| Accession number | Transposition | Origin | Host |
|---|---|---|---|
| NC_013860 | ND | Azospirillum sp. | Azospirillum sp. B510 plasmid pAB510f Azospirillum sp. B510 plasmid pAB510a |
DNA section
IS Length : 1322 bp
Ends
IR Length : 31
IRL : GGGACTATCCGGAATTTCGTGCTCGGCGGGGTTATCCTGAAGCAGAAGGA
IRR : GGGACTATCCGGAATTTCGTGCTCGGCGGGGCGGGTTTTAATCTCAGGCG
Insertion site
| Left flank | Direct repeat | Right flank | DR Length |
|---|---|---|---|
| CATGGGTCTCG | TTGGACCGC | TTCTCGCCGTG | 9 |
DNA sequence
GGGACTATCCGGAATTTCGTGCTCGGCGGGGTTATCCTGAAGCAGAAGGAGACCCTGAATGGCCCGACGCAAAGAACCGGTTATCCCCGACGCGATCCTC
GACCAGCTCCTGGCGGGGGCGGATGCCAAGACGGCGTTCGACCAGAACGGCCTGCTCGACCAGTTGAAGAAGGCGCTGACGGAACGGGCGCTGAAAGCGG
AGCTGGATCATCATCTGGCTGGGGACGAGAGCGGCAACCGGCGCAACGGCTACGGCCGCAAGACGGTGCTGACGGAGGGCGGGTCGATGGACTTGGCGAT
CCCGCGCGACCGGACGGGTACGTTCGATCCGGCGCTGATCGGCAAGTACCAGCGGCGGTTCCCCGGCTTCGATGAGAAGATCATCTCAATGTACGCACGG
GGCATGTCGACGCGGGAGATCACGGGGCACCTGCGGGAGCTCTATGGCATCGAGGTGTCGGCCGACCTGATCTCCACGGTGACGGATGCGGTGATCGACG
AGGTGACGACGTGGCAGAACCGGCCTTTGGAGGCGGTCTACCCGCTGGTCTTCCTGGATGCCATCCGGGTCAAGATCCGGGACGAAGGGCTGGTCCGCAA
CAAGGCCATCCATGTGGCCATCGGCGTGCGGGCCGACGGCGCCAAGGAGGTGCTGGGTCTGTGGATCGAACAGAACGAAGGCGCCAAGTTCTGGCTGCGC
GTCCTGAATGAATTGAAGAACCGGGGCGTTGAGGACATCCTGCTGGCTGTGGTCGACGGGCTGAAGGGCTTTCCCGAAGCGATCGCGGCGGCCTATCCCG
AGACGATCGTGCAGACCTGCATCGTCCACCTGCTGCGCCACAGCATGGAATTCGCGTCCTGGAAGGACCGCAAGGCCATCGCGGGCGCCTTGAAGACCGT
CTACGACGCTGTCGACGATAAGGCCGCCGAGGTGGCCCTGACCGCCTTCGAAGACGGACCCTGGGGCGAAAAGTATGCGGCCATCGGCAAGGCCTGGCGG
CGCGCTTGGCCGGAGGTCATTCCCTTTTTCGCCTTTCCCCGCGCGGTTCGACGCATCCTTTACACCACAAATGCCATCGAAGCGTTGAATTCCAAGCTGC
GCCGGGCGGTGCGGGCCCGAGGGCATTTTCCAAATGACGAGGCCGCGCTGAAGCTCTTGTTTCTGGTCTTGAACCGCTCCGAGAAAGATTGGAAGATGCC
GCCACGGGAATGGACGGCCGCCAAGGCACAGATGGCCGTCATGTTCGGCGAGCGTTTCTCAAAGGCGATGAGCGCCTGAGATTAAAACCCGCCCCGCCGA
GCACGAAATTCCGGATAGTCCC
GACCAGCTCCTGGCGGGGGCGGATGCCAAGACGGCGTTCGACCAGAACGGCCTGCTCGACCAGTTGAAGAAGGCGCTGACGGAACGGGCGCTGAAAGCGG
AGCTGGATCATCATCTGGCTGGGGACGAGAGCGGCAACCGGCGCAACGGCTACGGCCGCAAGACGGTGCTGACGGAGGGCGGGTCGATGGACTTGGCGAT
CCCGCGCGACCGGACGGGTACGTTCGATCCGGCGCTGATCGGCAAGTACCAGCGGCGGTTCCCCGGCTTCGATGAGAAGATCATCTCAATGTACGCACGG
GGCATGTCGACGCGGGAGATCACGGGGCACCTGCGGGAGCTCTATGGCATCGAGGTGTCGGCCGACCTGATCTCCACGGTGACGGATGCGGTGATCGACG
AGGTGACGACGTGGCAGAACCGGCCTTTGGAGGCGGTCTACCCGCTGGTCTTCCTGGATGCCATCCGGGTCAAGATCCGGGACGAAGGGCTGGTCCGCAA
CAAGGCCATCCATGTGGCCATCGGCGTGCGGGCCGACGGCGCCAAGGAGGTGCTGGGTCTGTGGATCGAACAGAACGAAGGCGCCAAGTTCTGGCTGCGC
GTCCTGAATGAATTGAAGAACCGGGGCGTTGAGGACATCCTGCTGGCTGTGGTCGACGGGCTGAAGGGCTTTCCCGAAGCGATCGCGGCGGCCTATCCCG
AGACGATCGTGCAGACCTGCATCGTCCACCTGCTGCGCCACAGCATGGAATTCGCGTCCTGGAAGGACCGCAAGGCCATCGCGGGCGCCTTGAAGACCGT
CTACGACGCTGTCGACGATAAGGCCGCCGAGGTGGCCCTGACCGCCTTCGAAGACGGACCCTGGGGCGAAAAGTATGCGGCCATCGGCAAGGCCTGGCGG
CGCGCTTGGCCGGAGGTCATTCCCTTTTTCGCCTTTCCCCGCGCGGTTCGACGCATCCTTTACACCACAAATGCCATCGAAGCGTTGAATTCCAAGCTGC
GCCGGGCGGTGCGGGCCCGAGGGCATTTTCCAAATGACGAGGCCGCGCTGAAGCTCTTGTTTCTGGTCTTGAACCGCTCCGAGAAAGATTGGAAGATGCC
GCCACGGGAATGGACGGCCGCCAAGGCACAGATGGCCGTCATGTTCGGCGAGCGTTTCTCAAAGGCGATGAGCGCCTGAGATTAAAACCCGCCCCGCCGA
GCACGAAATTCCGGATAGTCCC
Protein section
ORF number : 1
ORF 1
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 1221 bp | 406 aa | 59 | 1279 | + | No |
Chemistry : DDE
ORF sequence :
MARRKEPVIPDAILDQLLAGADAKTAFDQNGLLDQLKKALTERALKAELDHHLAGDESGNRRNGYGRKTVLTEGGSMDLAIPRDRTGTFDPALIGKYQRR
FPGFDEKIISMYARGMSTREITGHLRELYGIEVSADLISTVTDAVIDEVTTWQNRPLEAVYPLVFLDAIRVKIRDEGLVRNKAIHVAIGVRADGAKEVLG
LWIEQNEGAKFWLRVLNELKNRGVEDILLAVVDGLKGFPEAIAAAYPETIVQTCIVHLLRHSMEFASWKDRKAIAGALKTVYDAVDDKAAEVALTAFEDG
PWGEKYAAIGKAWRRAWPEVIPFFAFPRAVRRILYTTNAIEALNSKLRRAVRARGHFPNDEAALKLLFLVLNRSEKDWKMPPREWTAAKAQMAVMFGERF
SKAMSA
FPGFDEKIISMYARGMSTREITGHLRELYGIEVSADLISTVTDAVIDEVTTWQNRPLEAVYPLVFLDAIRVKIRDEGLVRNKAIHVAIGVRADGAKEVLG
LWIEQNEGAKFWLRVLNELKNRGVEDILLAVVDGLKGFPEAIAAAYPETIVQTCIVHLLRHSMEFASWKDRKAIAGALKTVYDAVDDKAAEVALTAFEDG
PWGEKYAAIGKAWRRAWPEVIPFFAFPRAVRRILYTTNAIEALNSKLRRAVRARGHFPNDEAALKLLFLVLNRSEKDWKMPPREWTAAKAQMAVMFGERF
SKAMSA
Blast result :
Comments
ISAzs19 is 95% aa similar to ISAzba8. The reference copy is disrupted by insertion of an ISAzs14 ( IS256 family). The sequence is reconstructed in silico by deletion of ISAzs14 and one of the direct repeat of this.
References
1] ISfinder annotation (2011)
2] Kaneko,T., Minamisawa,K., Isawa,T., Nakatsukasa,H., Mitsui,H., Kawaharada,Y., Nakamura,Y., Watanabe,A., Kawashima,K., Ono,A., Shimizu,Y., Takahashi,C., Minami,C., Fujishiro,T., Kohara,M., Katoh,M., Nakazaki,N., Nakayama,S., Yamada,M., Tabata,S. and Sato,S. (2010) DNA Res. 17 (1), 37-50.
2] Kaneko,T., Minamisawa,K., Isawa,T., Nakatsukasa,H., Mitsui,H., Kawaharada,Y., Nakamura,Y., Watanabe,A., Kawashima,K., Ono,A., Shimizu,Y., Takahashi,C., Minami,C., Fujishiro,T., Kohara,M., Katoh,M., Nakazaki,N., Nakayama,S., Yamada,M., Tabata,S. and Sato,S. (2010) DNA Res. 17 (1), 37-50.