ISAzs19

  • Family IS256
  • Group
MGE type ISRelated element(s) :
Isoform Synonym(s)
Accession numberTranspositionOriginHost
NC_013860 ND Azospirillum sp.
Azospirillum sp. B510 plasmid pAB510f
Azospirillum sp. B510 plasmid pAB510a
DNA section
IS Length : 1322 bp

Ends


IR Length : 31

IRL : GGGACTATCCGGAATTTCGTGCTCGGCGGGGTTATCCTGAAGCAGAAGGA
IRR : GGGACTATCCGGAATTTCGTGCTCGGCGGGGCGGGTTTTAATCTCAGGCG

Insertion site


Left flankDirect repeatRight flankDR Length
CATGGGTCTCGTTGGACCGCTTCTCGCCGTG9

DNA sequence

GGGACTATCCGGAATTTCGTGCTCGGCGGGGTTATCCTGAAGCAGAAGGAGACCCTGAATGGCCCGACGCAAAGAACCGGTTATCCCCGACGCGATCCTC
GACCAGCTCCTGGCGGGGGCGGATGCCAAGACGGCGTTCGACCAGAACGGCCTGCTCGACCAGTTGAAGAAGGCGCTGACGGAACGGGCGCTGAAAGCGG
AGCTGGATCATCATCTGGCTGGGGACGAGAGCGGCAACCGGCGCAACGGCTACGGCCGCAAGACGGTGCTGACGGAGGGCGGGTCGATGGACTTGGCGAT
CCCGCGCGACCGGACGGGTACGTTCGATCCGGCGCTGATCGGCAAGTACCAGCGGCGGTTCCCCGGCTTCGATGAGAAGATCATCTCAATGTACGCACGG
GGCATGTCGACGCGGGAGATCACGGGGCACCTGCGGGAGCTCTATGGCATCGAGGTGTCGGCCGACCTGATCTCCACGGTGACGGATGCGGTGATCGACG
AGGTGACGACGTGGCAGAACCGGCCTTTGGAGGCGGTCTACCCGCTGGTCTTCCTGGATGCCATCCGGGTCAAGATCCGGGACGAAGGGCTGGTCCGCAA
CAAGGCCATCCATGTGGCCATCGGCGTGCGGGCCGACGGCGCCAAGGAGGTGCTGGGTCTGTGGATCGAACAGAACGAAGGCGCCAAGTTCTGGCTGCGC
GTCCTGAATGAATTGAAGAACCGGGGCGTTGAGGACATCCTGCTGGCTGTGGTCGACGGGCTGAAGGGCTTTCCCGAAGCGATCGCGGCGGCCTATCCCG
AGACGATCGTGCAGACCTGCATCGTCCACCTGCTGCGCCACAGCATGGAATTCGCGTCCTGGAAGGACCGCAAGGCCATCGCGGGCGCCTTGAAGACCGT
CTACGACGCTGTCGACGATAAGGCCGCCGAGGTGGCCCTGACCGCCTTCGAAGACGGACCCTGGGGCGAAAAGTATGCGGCCATCGGCAAGGCCTGGCGG
CGCGCTTGGCCGGAGGTCATTCCCTTTTTCGCCTTTCCCCGCGCGGTTCGACGCATCCTTTACACCACAAATGCCATCGAAGCGTTGAATTCCAAGCTGC
GCCGGGCGGTGCGGGCCCGAGGGCATTTTCCAAATGACGAGGCCGCGCTGAAGCTCTTGTTTCTGGTCTTGAACCGCTCCGAGAAAGATTGGAAGATGCC
GCCACGGGAATGGACGGCCGCCAAGGCACAGATGGCCGTCATGTTCGGCGAGCGTTTCTCAAAGGCGATGAGCGCCTGAGATTAAAACCCGCCCCGCCGA
GCACGAAATTCCGGATAGTCCC
Protein section
ORF number : 1

 

ORF 1
LengthBeginEndStrandFusion ORF
1221 bp406 aa591279+No
ORF function : Transposase
Chemistry : DDE

ORF sequence :

MARRKEPVIPDAILDQLLAGADAKTAFDQNGLLDQLKKALTERALKAELDHHLAGDESGNRRNGYGRKTVLTEGGSMDLAIPRDRTGTFDPALIGKYQRR
FPGFDEKIISMYARGMSTREITGHLRELYGIEVSADLISTVTDAVIDEVTTWQNRPLEAVYPLVFLDAIRVKIRDEGLVRNKAIHVAIGVRADGAKEVLG
LWIEQNEGAKFWLRVLNELKNRGVEDILLAVVDGLKGFPEAIAAAYPETIVQTCIVHLLRHSMEFASWKDRKAIAGALKTVYDAVDDKAAEVALTAFEDG
PWGEKYAAIGKAWRRAWPEVIPFFAFPRAVRRILYTTNAIEALNSKLRRAVRARGHFPNDEAALKLLFLVLNRSEKDWKMPPREWTAAKAQMAVMFGERF
SKAMSA

 

Blast result :
Comments
ISAzs19 is 95% aa similar to ISAzba8. The reference copy is disrupted by insertion of an ISAzs14 ( IS256 family). The sequence is reconstructed in silico by deletion of ISAzs14 and one of the direct repeat of this.
References
1] ISfinder annotation (2011)
2] Kaneko,T., Minamisawa,K., Isawa,T., Nakatsukasa,H., Mitsui,H., Kawaharada,Y., Nakamura,Y., Watanabe,A., Kawashima,K., Ono,A., Shimizu,Y., Takahashi,C., Minami,C., Fujishiro,T., Kohara,M., Katoh,M., Nakazaki,N., Nakayama,S., Yamada,M., Tabata,S. and Sato,S. (2010) DNA Res. 17 (1), 37-50.