ISfinder Mirror Site

ISCja2

Family IS4
Group IS4Sa

MGE type ISRelated element(s) :
Isoform Synonym(s)

Accession number	Transposition	Origin	Host
NC_010995	ND	Cellvibrio japonicus	Cellvibrio japonicus Ueda107

DNA section

IS Length : 1274 bp

Ends

IR Length : 18

IRL : CACTGCTGTCCCATAGTTTGTAGATAAAAGAAAAGCCTGTTTTCCAAGTT
IRR : CACTGCTGTCCCATAGTTCTACTCATAAGGCGAGCCTCATTTGAGGCTCG

Insertion site

Left flank	Direct repeat	Right flank	DR Length
TGAATGCACT	CTTGCACTAC	TTGCGCTTGT	10
AATAAAAAGG	GCGCTGATGC	GCCCTTTTTA	10
GAACAGGGTA	GCCATCAGGC	TCGCTTTTTT	10
TAAAAAGGCC	GCGTATGCGG	CCTTTTTTAT	10
ACAAAAAAGG	TGGCATGGCC	ACCTTTTTTG	10
ATAAAAAAGC GGCATGACGC		GGCCTTGTGC CGGTTTTTTT	0
GTAAAAAGGC	GCTATTAGC	GCCTTTTTTG	9
ATAAAAAAGG	CAGCCACAGC	TGCCTTTTTT	10
AAAAAAGCCT	GCTCAGACT	TAGCTTTAAA	9
AAAAAGCACC	GCATTAGCGG	TGCTTTTTTT	10
GAAAAAGCCC	GGATATATTC	CGGGTTTTTT	10
TGCGGAAGGG	AACGCGCCCC	TGGTTTTTTT	10
ACAAAAAACC GGCCTTGTGC		GGCATGACGC CGCTTTTTTA	0

DNA sequence

CACTGCTGTCCCATAGTTTGTAGATAAAAGAAAAGCCTGTTTTCCAAGTTGATACAATGTAAGTGCGACCAAACATTGGTAAAACAAAGGAAAACAGGCT
TTGTCACATTCTAACACCGCATTTCATCAACTACTCAAACCTTTATCGAGACATGAATTTGAAGCGGAAGCTAAAAAGCATCATGTCGGCCAAAAATTGC
GCTCGGCCACCCGCTGGGATCAATTTGTCGGCATGGCCATGTCGCAGCTCTCTGGCCGCCAAAGCTTGCGTGATATTCAATCCAATCTCGAAGCACAGCA
GCATAAACTTTATCATCTCGGTGCCAAGCCAATAGCCCGTTCAACGTTGGCACGAATCAACGAAGTACAGCCCGCCGAACTCTACAAACACGTATTTGCT
CGTCTGCTTCACCGCTGTAAATCCATGCAGGGCAAACACAAATTCCAGTTTAAAAATCCGCTGTACTCCCTTGATGCCAGCGCGATTGATTTATCGCTGT
CGGTATTTCCCTGGGCGGCGCATCGAGATGACACAGCAAATGTAAAATTAAGTGTTGGCCTGAATCATGGCACCCAAGTGCCGGAGTTTGTCGCACTCAG
TGATGGCCAGGAAAACGACATGATTGAGGGACGAAAATTTGATTTTCCCAAAGGCAGCATCGTTGCGTTTGATAAAGGCTACGTGGATTACCGCTGGTTT
AAGTTATTGACAGATAAAGGAGTTTTCTTTGTAACCCGCTTGCGCGCCAAAGCCGTCTATCGCGTGGAAGAACGTCGCTATGCCGACAGCAGCAAAGGTA
TTATCAGTGATCAGGTCATTCAATTATCCAGCGCTCATGCGATCAAACGCGGCGCACCGAAGTTGCGTCGAATTGGCTATCGTGACGCAACAACCGGCAA
ATTCTATGAGTTTCTCACCAACAACTTTCAGCTGGCGGCGGCCACAATTGCAGCGATTTATAAGGATCGCTGGCAGGTGGAGTTGTTCTTCAAAGCGATC
AAACAAAACCTGAAGATCAAAGCGTTTGTGGGCACTTCAAGAAATGCGGTGTTAACACAAATTTGGATTGCCATGATTACGTATCTATTGTTGGCATTTT
CGCGTCACAGCACTAAAGCGGGATGGACAGTTCAACGGATCATGAGGGTAATTCAGTTAAATTTGTTTGAGCGTAGGAGTCTAAAAAGCATCCTGATACC
GGACCCGCCGAATCATAAAAAAAGCGAGCCTCAAATGAGGCTCGCCTTATGAGTAGAACTATGGGACAGCAGTG

Protein section

ORF number : 1

ORF 1

Length		Begin	End	Strand	Fusion ORF
1152 bp	383 aa	101	1252	+	No

ORF function : Transposase
Chemistry : DDE

ORF sequence :

MSHSNTAFHQLLKPLSRHEFEAEAKKHHVGQKLRSATRWDQFVGMAMSQLSGRQSLRDIQSNLEAQQHKLYHLGAKPIARSTLARINEVQPAELYKHVFA
RLLHRCKSMQGKHKFQFKNPLYSLDASAIDLSLSVFPWAAHRDDTANVKLSVGLNHGTQVPEFVALSDGQENDMIEGRKFDFPKGSIVAFDKGYVDYRWF
KLLTDKGVFFVTRLRAKAVYRVEERRYADSSKGIISDQVIQLSSAHAIKRGAPKLRRIGYRDATTGKFYEFLTNNFQLAAATIAAIYKDRWQVELFFKAI
KQNLKIKAFVGTSRNAVLTQIWIAMITYLLLAFSRHSTKAGWTVQRIMRVIQLNLFERRSLKSILIPDPPNHKKSEPQMRLAL

Blast result :

Comments

ISCja2 is 83% aa similar to ISOsp1. There is an exchange of direct repeat between these two copies of the IS : (2118754-2120027) and COMPLEMENT (2337006-2335733).

References

1] ISfinder submission (2011)
2] Deboy,R.T., Mongodin,E.F., Fouts,D.E., Tailford,L.E., Khouri,H., Emerson,J.B., Mohamoud,Y., Watkins,K., Henrissat,B., Gilbert,H.J. and Nelson,K.E. (2008) J. Bacteriol. 190 (15), 5455-5463.