ISfinder Mirror Site

ISAzs18

Family IS66
Group

MGE type ISRelated element(s) :
Isoform Synonym(s)

Accession number	Transposition	Origin	Host
NC_013855	ND	Azospirillum sp.	Azospirillum sp. B510 plasmid pAB510a Azospirillum sp. B510

DNA section

IS Length : 2633 bp

Ends

IR Length : 18/22

IRL : GTAAGCGTCGTCTGAGTGCTGCCTTGCGGGAGGAGTGTGCCGCGTGCTGA
IRR : GTAAGCGTCGTCTGGCGACTGCGGCGGAATTGGGTTGACGGCTCCGCTTC

Insertion site

Left flank	Direct repeat	Right flank	DR Length
CTGTCCTGAAAGTTTATTTC		CACCGCACTCGCCAGCCGGC	0
TCTCCGAAGCCGTGTAGCTC		TCCAAATCCACCGAGCAGCACG	0

DNA sequence

GTAAGCGTCGTCTGAGTGCTGCCTTGCGGGAGGAGTGTGCCGCGTGCTGAAAGCTGGACGTCGGCGGCACCGGTGGGTGTGGCTGGCTTGGCATCGTGGC
AAGCAGCGTGGTTTGCACTGTGGCAGTCCAACGTATCGAGGTCATCACCGGCGCCGGCGGGCGGCGCACCTACTCGGCCGAGGAGAAGATCCGCTTGGTC
GGCGAGGCCCACGGCGGGCGCGGCGCCGTCGTCGCGGTGGCCCGCCGCCACGGCGTGTGCACCAGCCTGATCTACCGCTGGCGCCGACAGTTCAAGAGCG
GAGAACTGTCTGCCGCGGCGCCCTCCTTCGTGCCGGTTCATGTGTTGGAGGGGCCGCCGTCCGCCGGCCTGCTCTCTCCCACCAAGACGCCGTCGGCGCC
GGAGCCGGCACCCTCAGCCGAGCGCCGTGCCGCCCTGGTCGAGGTGGTTCTCGCCAACGGCCGCGTGCTGCGCGTCGCCGAGGATATCGCCCCGGCCACG
CTGCGCCGACTGGTCGGCGCGTTGGATTCGCCATGATCTCGGTTCCCGCCGGGGTGCGGGTCTATCTGGCGATGGGCGCCACCGACATGCGCAAGGGCAT
GGACGGGTTGGCCATGCTCGCCCAGCAGGTCCTTCAGCAGGACCCGTTCGCCGGCCATTTGTTTGTTTTCCGGGGTCGGCAAGGTCATCTGGTGAAGGTT
CTTTACTGGGATGGCCAGGGCTTCTGCCTGTTCACCAAGCGCCTGGAGAAGGGGCGCTTCGTGTGGCCGATCAGCCGCGAGGGCGTGGCGGTGCTCACTC
CGGCCCAGTTGGCGATGCTCATCGAGGGCATGGATTGGCGCGCCCCGCAGCGCACATGGCGCCCGGAGATGGCGGGGTGAGTCCCGGCCGCCACGCCCGC
CGATGATCGGCGCCGGGGTGGTGATCGCTCCTCCTCCGGGAGCCGCCGATCGGGTAGACTCCGGCCATGCCCGCTACCGCCGAGCCGCTGCCCACCGACA
TTGAGACGCTGCGCGCGATCATCGCGGCGCAGGCCGCGGAGTTGGCGGCCGAGCGTCGGTGTCGTGAGGCCAGCGAGGCCGAATTGGCCGCCGCCAAGGC
CGGCCTGGTGGCCAAAGCGCTGGAGGTGGAGAAGCTCAAGATCCAGTTGGCTCGGCTGCGGCGCATGCAATTCGGGCGTTCCTCCGAGAAGATCGACCGC
GAGATCGACCAGCTCGAACTGGCGCTGGAGGATCTGGAGGCTGCCGCGGCGGCCGAAGGGATGCCGGCCGATCCCGCCGAGCCGCGGGCGCCCTCGGCGG
AGAGCCGTGCCCAAGCCGGCCGGCGCAAGCTGCCGGAGCATCTGCCGCGCACCGAGATCGTGCACGCCCCGTTTGAGGCCTGCCCCTCCTGCGGCGGCGC
CCTGCGTCCGGTGGGCGAGGATGTCCGCGAGGTTCTGGACTACATCCCGGCGCGTTTCACCGTGATCCGCCACGTCCGCCCGGCGCTGTCGTGCCGGTGC
TGCGAGGGCATGGTGCAGGCGCCGATGCCGTCGCTGCCCATCGAGCGCGGCCTGCCCTCGGCCGGGCTGCTGGCGCATGTGCTGATCGCCAAATATTGCG
ATCACCTGCCGCTCTATCGGCAATCGGCCATCTACGCCCGCGAAGGCGTGGAATTGGAACGCTCGCTGCTGGCCGACTGGGTCGGCAAGGCGGCGGCGCT
GATGGCGCCGCTGGCCGAGGCCATTGGCCGGCACGTGCTGGCCGGTTCGGTTCTACACGCGGACGACACGCCGGTGCCGGTGCTCGATCCCGGGCGTGGC
ACGACCAAGACGGGGCGACTGTGGGTCTATCTGCGCGATGAACGCCCGCACGCCGGACAAGGGCCGCCGGCGGTGCTGTACCGCTATACCCCCGACCGCA
AAGGCGAACATCCGCAACGGGAGCTGGCTGGCTTCCGTGGGCACCTGCATGCCGACGGCTATGGCGGGTTCGATGCTCTGTACGAAGCCAAGGGCGGCAA
ACCGGCCGCTGTCGCCGAAGTGGCCTGTATGGCTCACGTGCGCCGGAAATTCTTCGACGAGCACACCGAAACCGGCTCGCCCCTGGCCGCCGACGCGCTG
AAACGGATCGGCAGGCTGTTTGACATCGAACGCCCCCTGACCGGCCAGCCCGCCGAGGACCGCCGCCGGATTCGCCAAGCGGAAGCCCGGCCCGTTCTCG
ATGAACTGGCCACCTTCCTCGACGCCGCCTTGGTGCGCATCCCCGGCAAGGGCGACCTGGCCAAGGCGATCCGCTACGCCCGCTCGCGCTGGACGGCACT
GACGCGCTACGTGGACGACGGCCGGCTGGAGAACTCCAACAACGCCGCCGAACGAGCGATCAGGCCGCTGGCCATCGGAAGACGCAACTGGATGTTCGCC
GGTTCCGACGCCGGCGGGGAGCGTGCCGCGGCGATTTATACCATCGTGGAATCCGCCAAACTCAACGGCCTCGATCCCCAGGCCTACCTGCGACATGTGC
TCGACCGCATCGCGGATCACCCGATCAACCGAATCGCCGATCTCCTGCCCTGGAACCTCACGATTCCCGACCACGGGTGAACGGAAGCGGAGCCGTCAAC
CCAATTCCGCCGCAGTCGCCAGACGACGCTTAC

Protein section

ORF number : 3

ORF 1

Length		Begin	End	Strand	Fusion ORF
441 bp	146 aa	96	536	+	No

ORF function : Accessory Gene
AG : IS66 TnpA

ORF sequence :

MASSVVCTVAVQRIEVITGAGGRRTYSAEEKIRLVGEAHGGRGAVVAVARRHGVCTSLIYRWRRQFKSGELSAAAPSFVPVHVLEGPPSAGLLSPTKTPS
APEPAPSAERRAALVEVVLANGRVLRVAEDIAPATLRRLVGALDSP

Blast result :

ORF 2

Length		Begin	End	Strand	Fusion ORF
348 bp	115 aa	533	880	+	No

ORF function : Accessory Gene
AG : IS66 TnpB

ORF sequence :

MISVPAGVRVYLAMGATDMRKGMDGLAMLAQQVLQQDPFAGHLFVFRGRQGHLVKVLYWDGQGFCLFTKRLEKGRFVWPISREGVAVLTPAQLAMLIEGM
DWRAPQRTWRPEMAG

Blast result :

ORF 3

Length		Begin	End	Strand	Fusion ORF
1614 bp	537 aa	967	2580	+	No

ORF function : Transposase
Chemistry : DDE

ORF sequence :

MPATAEPLPTDIETLRAIIAAQAAELAAERRCREASEAELAAAKAGLVAKALEVEKLKIQLARLRRMQFGRSSEKIDREIDQLELALEDLEAAAAAEGMP
ADPAEPRAPSAESRAQAGRRKLPEHLPRTEIVHAPFEACPSCGGALRPVGEDVREVLDYIPARFTVIRHVRPALSCRCCEGMVQAPMPSLPIERGLPSAG
LLAHVLIAKYCDHLPLYRQSAIYAREGVELERSLLADWVGKAAALMAPLAEAIGRHVLAGSVLHADDTPVPVLDPGRGTTKTGRLWVYLRDERPHAGQGP
PAVLYRYTPDRKGEHPQRELAGFRGHLHADGYGGFDALYEAKGGKPAAVAEVACMAHVRRKFFDEHTETGSPLAADALKRIGRLFDIERPLTGQPAEDRR
RIRQAEARPVLDELATFLDAALVRIPGKGDLAKAIRYARSRWTALTRYVDDGRLENSNNAAERAIRPLAIGRRNWMFAGSDAGGERAAAIYTIVESAKLN
GLDPQAYLRHVLDRIADHPINRIADLLPWNLTIPDHG

Blast result :

Comments

ISAzs18 is 71% (ORFC : the transposase) aa similar to ISPre3.

References

1] ISfinder annotation (2010).
2] Kaneko,T., Minamisawa,K., Isawa,T., Nakatsukasa,H., Mitsui,H., Kawaharada,Y., Nakamura,Y., Watanabe,A., Kawashima,K., Ono,A., Shimizu,Y., Takahashi,C., Minami,C., Fujishiro,T., Kohara,M., Katoh,M., Nakazaki,N., Nakayama,S., Yamada,M., Tabata,S. and Sato,S. (2010) DNA Res. 17 (1), 37-50.