ISAzs17

  • Family Tn3
  • Group
MGE type ISRelated element(s) :
Isoform Synonym(s) TnAzs17
Accession numberTranspositionOriginHost
NC_013854 ND Azospirillum sp.
Azospirillum sp. B510
DNA section
IS Length : 7252 bp

Ends


IR Length : 36

IRL : GGGGTCACTACACGAAAGTGATCAGATTGTCCGGGATCTGAGAACAGCCT
IRR : GGGGTCACTACACGAAAGTGATCAGATTGTCCGGGAAGTGGGAACGGAAA

Insertion site


Left flankDirect repeatRight flankDR Length
GCGCCGCCTGCACGAGGTGCTGCGCGGTCAGGGA5

DNA sequence

GGGGTCACTACACGAAAGTGATCAGATTGTCCGGGATCTGAGAACAGCCTGAGAACATGAGCCGTGCAGCGATCCTGTGCCGGCTGCTTGCCTCGCAAAA
CTCTTGAGTTTCGCGACAGGTAGGTACCGGACTGGAACCTTGGAGTTTTTGATGTTGCAAAAGTCTATCGCAAAAGGAAAGAGTTTCGCGATGACTTTTG
CGACGGATGCCCCATGCTCATCGGCTATGCGCGCGTCTCCACCGACGACCAGGATCTTTCGCAGCAGCGGGCGTCGCTACAGGCGGTCGGCTGTCGGCGG
ATTTATGAGGAGAAGGTCTCCGGGGCAAAGCGCGACCGGCCACAGCTTGCCCGGCTGATCGACCAACTCCGGGCCGATGACGTTGTGACGGTCACGCGCC
TCGACAGGCTGGCCCGGTCAACCCGTGATCTTCTCGATATTGCGGAGCAGCTCCAGGGAGCCGGTGCGGGTCTCCGATCACTCGCGGAGCCATGGGCCGA
TACCACCTCGCCGGCCGGGCGGATGGTCCTAACCGTGTTCGCCGGCATCGCCGAGTTCGAGCGCTCCCTGATCGCCGAGCGGACCGGCATGGGCCGCGTG
GCGGCCAGAGCACGCGGCGTGCGGTTCGGCCGGCCGCCGAAGTTGACCGCCGACCAGATCGCTCTCGCTCGGCGCCTGGTCGATGAAGGCCGATCCCCCC
GGGAGGCAGCCCGGATTCTCAACGTCCACGCATCGACGCTGTACCGGGCCTTTGCACTCCAGCCCCCCGAGGTTGCTGGCTCAGGTCCCTAACCGGGCTA
TGTCAGATTTCGTTCCTTTGCTACTCGATCTTCGACCAGCCCCTGCCGAAATGCCGTCATGCTGTCTTCTCACCGCTCATCGCAGCGGCGGCCAGGGCCT
GCGCCTCGGTTGCCGCCTTCAGCCCCGCCTCGCGGCGCTCGCTGTAGCGGTCGGTCAGGTAGTCGCTGCGGTCGCGCACCAGCAGAGTGAACTTCATCAG
TTCCTCCATCACGTCCACCACGCGGTCGTAATAGGGCGACGGCTTCATCCGCCCGGCCTCGTCGAACTCCTGGAAGGCCTTGGCCACCGACGACTGGTTC
GGGATGGTCACCATCCGCATCCAGCGGCCTAGCACCCGCATCGCGTTGACCGCATTGAAGGATTGCGAGCCGCCGGAGACCTGCATCACCGCCAGCGTCC
GCCCCTGCGTCGGCCGGACGCCGCCGCTTTCCAGCGGCAGCCAGTCGATCTGGCATTTCAGGATGCCGGTGATGGTGCCGTGCCGCTCCGGGCTGACCCA
AACCTGGCCTTCCGACCATTGCGACAGGGCGCGCAGCTCCGCCACCTTGGGATGGTCGGCCGGCACGCTGTCCGGCAGCGGCAGGTCGCGCGGGTCGAAG
ATGCGCGTCTCGGCCCCCATGCGCTGGAGCAGCCGGGCGGCTTCCTCGGCCAGGAAGCGGCTGTAGGACCGCTCCCGCAGCGAGCCATAGAGCAGCAGGA
TGCGCGGCGGGTGGGTCGACGGCCGCTCCGGTGTCAGCCTGTCGAGGCCGGGCACATCGAAATAAGGCTCGTCCAGGCTCGGCAAGGAGTCGCGTCCGGT
CGTCTCGATATCATGCATGGGTGCGTCCCTCGTACCAGGGCTTGGTCGCCTTGACGATCTTCACCACAGACAGCATCACCGGCACCTCGACCAGCACGCC
GACCACGGTGGCGAGCGCCGCCCCGGAGCCCAAACCGAACAGGCTTATGGCGGCGGCGACCGCCAGCTCGAAGAAGTTGGAGGCGCCGATCAGCGCCGCC
GGCGCTGCCACGCACCACGCCACGCCGAAGCGGCGCGACAGCCAATAGGCCAGCCCGGCGTTGAAATAGACCTGGATCAGGATCGGCACCGCCAGCAGCA
GGATGACCAGCGGCTGGGCGAGGATCTGCTCGCCCTGGAAGCCGAACAGCAGCACCAGCGTGGTCAGCAGCGCCAGCAGCGAAACCGGCTGGATGAGGTC
GAGCACGCGCTTCAGCGCCGGCTCTCCGCCGGCGACCAGCAGGCTGCGCCGCCAGAGCTGGGCACCGACCACGGGGATGACGATGTAGAGCAGCACCGAC
AGCAGCAGCGTGTCCCACGGCACGGTGATCGACGCCACGCCCAGCAGCAGGCCGACCAGCGGCGCGAAAGCGAAGACCATGATGATGTCGTTCAGCGCCA
CCTGGCTCAGCGTGTAGTGCGGCTCCCCCTCACACAGGTTCGACCAGACGAACACCATGGCGGTGCAGGGCGCCGCCGCCAGCAGGATCAGGCCGGCAAC
GTAGGAGGAAATCTGGTCCTGCGGCAGCAGCGGCGCGAACAGGTTGCCGATGAACAGCGTGCCCAGCAGCGCCATCGAGAAGGGCTTGACCGCCCAGTTG
ATGAACAGCGTCACCCCAACGCCGCGCCAGTGCTCCTTGACCTGCCCCAGCGCGCCGAGGTCGATCTTCAGCAGCATCGGCACGATCATCAGCCAGATCA
GCACCGCCACCGGCAGGTTGACCTTGGCGACCTCGGCCGCGGCGATCGTCTGGAACAGGCCGGGCACCAGATGGCCGAGCGCGATGCCGGCGACGATGCA
CAGCGCTACCCAGACGCTGAGATAGCGCTCGAACGGGCCCATGGCGGGGCGGGCCGCCGGGGCGGCATGAATGTCAGCGGTCATGATGCGTGTCCGTCAG
GAGGCTTTGCCGATGCGGTGTCCGGCGGCGTCGACGACCTGCTCCCCGTCTTCCTTGGCGAAGGCGCCGCGCTGGGCATCGGGGAGGATGTCGAGCACCG
CCTCGGACGGGCGGCACAGCCGCACGCCGAGCGGCGTCACCACGATCGGCCGGTTGATCAGGATCGGATGGGCCATCATGGCGTCGAGCAGTTGGTCGTC
GCTCAACGACGGGTCGGCGAGCCCGGGTTCGGCATAGGGCGTGCCCTTCTCGCGCAGCACGGCGCGCACCGGCACGCCCATGCGGCCGATCAGGTCGGCC
AGCTCGTCACGCGACGGCGGTGTCTTCAGATACTCGATCACCGTCGGCTCGACGCCGCTGTTGCGGATCAGCGCCAGCGTGTTGCGCGAGGTGCCGCAAT
CCGGGTTGTGGTAGATGGTCACCGATGTCATCGCGCCGCCTCCGTGGTGGCGGACGCCTTCCCCATGTCGTCCAGCTTGCGCTTCAGCGCCAGACGGTCG
AGCGAGGCGATGGGCAGGGCGGTGAAAGCCTGGATGCGGCGCTGGATCTGTCCGAACACCGTGGCGGTGAAGGCGGCCTTCTCCGCGTCGCTGCCCTGGG
CAGTCGACGGGTCTGGGAAGCCCCAGTGCGCCGTCATCGGCTGGCCGGGCCACACCGGGCAGACCTCGCCGGCGGCGTTGTCGCAGACGGTGAAGACGAA
GTCCATGTGCGGCGCGTCCGCTGCGGCGAACTCGTCCCAGGTCTTGGAGCGCAGCTCGGCGGTCGGGAAGCCCAGCCGCTCCAGCAGGTCATGGACATAG
GGATTGATGCGGCCGGACGGCTGGCTGCCGGCGCTGAAGCCGCGGAAGCGGCCCTTGCCATCGCGGTTCAGCAGGCACTCCGCCATGACGCTGCGGGCGC
TGTTGTGGGTGCACAGGAACAGGACGTTGTAGACGCGGTCGGTCATCGCTGGATTGCCTTTCCGGGCCGCGGGATGCGGCGGTTGGGATCGGGGCCTCAG
GCGTCGTGGTCACATCCGTCGGGACCACATGGAACGGCCTGGGGGAGGTCGGCGGAGCAGAGTTCGGGGCGGCCCTGGCAGCAGTCCTCCATCAGGAAGG
CGATCAGCCGGCGGGTGCCCTCATAGTCGGACGCGTAGAAGATCTGCCGCTGCACCCGCCAGGAGCGGAGCAGGCCGGCGCGTTCCAGCGTCGCCAGATG
ATGGGACAGGGTGGAGGCCGGAACCCCCACCTGCTGGGCAACCTCGCCTGCCGTCAACCCGTTCGGCCCCACCGTGATCAGCAGGCGGAACACGGCCAGA
CGGGTTTCCTGGGCGAGAGCGGCAAAGGCGTCGACGGCAATCTTGTTTTCCATTATTCGACGATAGTCGAATTATCGATTTGAGGCAATCAGCAGTTTTG
CGCGCCATGCGGTGAGGGGACGCGGGAAAACCGGAAGCAGCGCCTCCGACAGCTCACCCCGTGACAGCCTTGGACCCGCATCACTATTCTCCCCCGAAAA
CGAAGCGATGGGGGATTGGAAGAATGGCGCGTCGGCGGCTGTTGACCGAAGACCAGTGGGCAAGCCTCCTGGCAGTGCCGGACGACGAACGCGCGATGGT
GCGCCACTATACGCTCAGCAGGGACGATCTCGACATCGTCACCCTCCGGCGTACGGCGCACACCCGCCTGGGCTGTGCCATTCTGCTCTGTTATCTCCAT
CATCCTGGGCGGATTCCTGGACCGGACGAGCGCCCGCCCATGGCGCTGCTGGTGTTCGTCGCCCGGCAGGTGGGCGCCGAGCCTGACGATTTCATCGCCT
ACAGTCGACGTGACCAGAACCGGCGCGAGCAGGTTGCCGCGATCATGGCACGGACCGGCCACCGTGCCTTTGATCGAACCCTCTTTCGCGACCTTGCCGC
CTGGCTGACCTCCAAGGCACAGATCGCACGGGACCCGATCATTCTGGCGACCACCTTGATCGATGAGTTCCGTCGCCGTCGCATCCTGATCCCTTCTGCG
GCGATCCTGGAACTGATGCTCCATCAGGCTCGGGGCCGGGCCGAACGGGTTCTGCACCGTGCCCTTGTCGATGGTGTCGACCAGCGGACAACCGACGCGC
TGGACCGATTGCTGACCCTGCAGGCCGACACCGAAACGACCATGCTCGCTTGGTTGCGCCGGGTGCCGGCGGCTCCAACCGCCCGCAACATGCTGGCGGT
CATTGAGCGCCTTTCCACCTTGAAGGAACTCGGCATCGACCGGTCGTTGCGCGCTCGCGTGCCGGAGGTCGCGTTCGAGCGGCTCGCGGCCGAAGGCCTG
CGGATGACGCCGCAGCATCTTCAGGATCTGGCGACGTCGCGCCGGCGTGCTGTGCTCACGGCCACCGCGATCCGCCTGGAGATGGATCTGACCGATGTTA
CCCTGTCGATGTTCGACAAGCTGATCGGCAGTCTGGCACGGAAGGCCGAGCGCCGGACGGCGGAAAACACCCTGCGTTCCGTCCGCGATACCCAGACCCA
ACTGCGGGTTCTGCTGACCGCGTGCAAGGCAGTGATCGGCGCGCGGGAGGCTGGCGCCGATCCCTACGAGGCCGTCGACCAAGGGGTCGGCTGGTTCCGT
TTCATGAAATGCGTCACCGACAGCGAAGCCCTGGCGAAGCCGGAGATCGCCGACCCGCGCACCGAGATGCTTGGCCGTTACGCCACCGTTCGAGCCTTCG
CCCCCACGCTCCTCAATCGCTTCAGCTTTCTCGGCAGTCCATCGGTGACGCCGTTGCTGCGGGCGCTCGACATCATTCGGGTCATGTACGCCGCCGGGCG
CCGCACTCTGCCCGAGAAGCCACCGACCGGCTTCATCCGACGCTCCTGGCGCCCCTTCGTCATCAAGAAGGAGGGCATCGACCGGAAAGCTTACGAAATC
TGCGCATTGTCCGAACTGCGTGACCGGTTGCGGGCGGGCGACGTCTGGGTGGAGGGGAGCCGCCAGTACCGCGACTTCGAGAGCTGCCTCATGCCGCGTC
CGACCTTCGATATCCTTTGCGAGGAGGGGGCGTTGCCCGTGGCCGTTCCACGCAGCGCCGACGGCCACCTTGCTGAAAGAGGCGCGGAACTCGACCGGAT
TGTTGCCGAAGTAGCGGACCTTGCCGAAGAGGGGTTGCTTGAAGACGTCGACCTGTCCGATGGCGAACTCCGCGTCACGCCGATCCGGGACACGACCCCA
CCGGAGGCCGAAGACCTTGGCCGCCGAGCTTACGATCTCATGCCACGGGTCAAGATCACCGACCTTCTGCAGGAGGTGGATGGCTGGACCGACTTCTCCG
GCTGCTTCACGCACCAACGCAGCGGCCGTCCCGCTGACGACAGGGCGGCAACATTGACCGCTGTGCTGGCCGACGGCATCAACCTCGGGCTGTCCCGGAT
GGCCGAAGCCTGCCGGGGGGCATCCATGCGGCAACTGGCCTGGATGCACGACTGGCACATCCGCGACGATACCTACATGGTGGCGCTTGCCCGCATCATC
GACACGCACCGAGCCTTGCCGTTGGCTGCCGTCTGGGGTGACGGGCGCACGTCATCCTCCGACGGCCAATTCTACCGGGCTGGCGGTCGCGGCGAAGCCG
TCGGCGATGTGAACGCCCATCATGGCAACGAGCCGGGCATCGCCTTCTACACCCACGTCTCCGATCAGTACGGCCCCTTCCACACCAAGGTGATCGCAGC
GACCGCCAGCGAGGCACCCCACGTCATTGATGGCCTTCTCCATCACCAGAGCGGTTTGGACATCGAGGAGCACTACACGGACACCGGAGGCGCAAGCGAT
CACGTTTTCGGCCTGTGTGCCCTGCTCGGATTCCGTTTCGCCCCGCGCATCCGCGATTTGAAGGACCGGAGGCTTTACCTGCTGCCCGGTCGGGAGGCCC
CCGCCATTCTGCGGCCCTTGGTTGGCGGAACCATCGACATCGAGCACCTCACGGCCAATTGGACGGAATTGTTGCGACTGGCGACCTCGATCCGCTCGGG
CACCGCGACGGCCTCGGCCATGCTGCGGCGGCTCTCGGCTTACCCGCGCCAGAATGGCTTGGCCCTGGCTCTGCGAGAACTCGGCCGTATCGAGCGGACC
GCGTTCACCTTACAGTGGCTGCGTGATCCCGCCCTCCGCCGGCGCGCCAACGCCGGCCTCAACAAGGGAGAGGCTCGGAACGCCCTGGCCAGGGCGATCT
TCTTCAATCGCCTGGGTGAGATGCGTGATCGCAGCTTCGAGAACCAGGTCTTCCGGGCATCAGGGCTGAACCTGCTGGTGTCCGCCATCATCCTGTGGAA
CACACGCTATCTCGAAGCCGCTTTCGCCGAACTGGCAAGCCAAGGGCATGGCGTAACCCCCGAATTAATGCGGCATGTGGCGCCTCTGGGATGGGAGCAT
GTCGGCCTCACCGGTGACTATGTCTGGGGAGCCGAACCCCTTCCAGAAGCCGGCCTCAGGCCATTGCGGAGAATGCAGTCCCTGCTCGCCGCATAAGTTC
CATTTCCGTTCCCACTTCCCGGACAATCTGATCACTTTCGTGTAGTGACCCC
Protein section
ORF number : 2

 

ORF 1
LengthBeginEndStrandFusion ORF
579 bp192 aa214792+No
ORF function : Accessory Gene
AG : Tn3 resolvase

ORF sequence :

MLIGYARVSTDDQDLSQQRASLQAVGCRRIYEEKVSGAKRDRPQLARLIDQLRADDVVTVTRLDRLARSTRDLLDIAEQLQGAGAGLRSLAEPWADTTSP
AGRMVLTVFAGIAEFERSLIAERTGMGRVAARARGVRFGRPPKLTADQIALARRLVDEGRSPREAARILNVHASTLYRAFALQPPEVAGSGP

 

Blast result :
ORF 2
LengthBeginEndStrandFusion ORF
2973 bp990 aa42247196+No
ORF function : Transposase
Chemistry : DDE

ORF sequence :

MARRRLLTEDQWASLLAVPDDERAMVRHYTLSRDDLDIVTLRRTAHTRLGCAILLCYLHHPGRIPGPDERPPMALLVFVARQVGAEPDDFIAYSRRDQNR
REQVAAIMARTGHRAFDRTLFRDLAAWLTSKAQIARDPIILATTLIDEFRRRRILIPSAAILELMLHQARGRAERVLHRALVDGVDQRTTDALDRLLTLQ
ADTETTMLAWLRRVPAAPTARNMLAVIERLSTLKELGIDRSLRARVPEVAFERLAAEGLRMTPQHLQDLATSRRRAVLTATAIRLEMDLTDVTLSMFDKL
IGSLARKAERRTAENTLRSVRDTQTQLRVLLTACKAVIGAREAGADPYEAVDQGVGWFRFMKCVTDSEALAKPEIADPRTEMLGRYATVRAFAPTLLNRF
SFLGSPSVTPLLRALDIIRVMYAAGRRTLPEKPPTGFIRRSWRPFVIKKEGIDRKAYEICALSELRDRLRAGDVWVEGSRQYRDFESCLMPRPTFDILCE
EGALPVAVPRSADGHLAERGAELDRIVAEVADLAEEGLLEDVDLSDGELRVTPIRDTTPPEAEDLGRRAYDLMPRVKITDLLQEVDGWTDFSGCFTHQRS
GRPADDRAATLTAVLADGINLGLSRMAEACRGASMRQLAWMHDWHIRDDTYMVALARIIDTHRALPLAAVWGDGRTSSSDGQFYRAGGRGEAVGDVNAHH
GNEPGIAFYTHVSDQYGPFHTKVIAATASEAPHVIDGLLHHQSGLDIEEHYTDTGGASDHVFGLCALLGFRFAPRIRDLKDRRLYLLPGREAPAILRPLV
GGTIDIEHLTANWTELLRLATSIRSGTATASAMLRRLSAYPRQNGLALALRELGRIERTAFTLQWLRDPALRRRANAGLNKGEARNALARAIFFNRLGEM
RDRSFENQVFRASGLNLLVSAIILWNTRYLEAAFAELASQGHGVTPELMRHVAPLGWEHVGLTGDYVWGAEPLPEAGLRPLRRMQSLLAA

 

Blast result :
Comments
ISAzs17 is 59% (ORFA : the resolvase) aa similar to ISSod9 and 61% (ORFB : the transposase ) to ISAcsp1.
Between the resolvase and the transposase, there is an operon arsenic with 5 genes :
arsenical resistance protein : 253(1618-857) CS
arsenite transporter ACR3 family : 357(2684-1611) CS
arsenate reductase : 143(3131-2697) CS
transcriptional regulator : 172(3646-3128) CS
transcriptional regulator : 118(4053-3697) CS
References
1] ISfinder annotation (2010).
2] Kaneko,T., Minamisawa,K., Isawa,T., Nakatsukasa,H., Mitsui,H., Kawaharada,Y., Nakamura,Y., Watanabe,A., Kawashima,K., Ono,A., Shimizu,Y., Takahashi,C., Minami,C., Fujishiro,T., Kohara,M., Katoh,M., Nakazaki,N., Nakayama,S., Yamada,M., Tabata,S. and Sato,S. (2010) DNA Res. 17 (1), 37-50.