ISAzs16

  • Family IS5
  • Group IS1031
MGE type ISRelated element(s) :
Isoform Synonym(s)
Accession numberTranspositionOriginHost
NC_013854 ND Azospirillum sp.
Azospirillum sp. B510
DNA section
IS Length : 990 bp

Ends


IR Length : 16/17

IRL : GAGGCTGTCCGGTAAAATATTGACGAGATTGGGCTCGAATTTTTTGCGGG
IRR : GAAGCTGTCCGGTAAAAGGTGGATCGAGTTTTTGGCTAATTTGGGAGGGC

Insertion site


Left flankDirect repeatRight flankDR Length
GCATCCCCGATGTGGACTGATTACTCCTCCGGGAAACGGA0
TCACGCCGCCAGCCCCATAATTACAGTGCACTTATGGGCC0

DNA sequence

GAGGCTGTCCGGTAAAATATTGACGAGATTGGGCTCGAATTTTTTGCGGGCAACGGGGCGTTGCCGGGAGTGTTGATCCGATATCCCTTTACGGTGGTGG
AGGCCTGTCATGCCATGCCCACACGATCCCTATCCGTCTGACCTCAGCGACGATGAATGGGAAGTCGTCGCACCTCTCTTTTCCAAGAGCGAAAGGCGGG
GACGGAAGCCGATCTATGATCTGCGCCGGATCTTGGACGGCTGCTTCTATGTTTTGCGCGGCGGAATTGCTTGGCGGATGATGGCGCATGACTTGCCGCC
GTGGCGCGTAGTCTACGATCACTTCAGCCAATGGCGCAAGACTGGCAAGTGGGAGCGGATCAATGAGGCCTTACGTGAGCGTTACCGCGTGAGTCGGGGG
CGTGATCCCCAGCCGTCGGCCGCCGTCATCGACAGCCAATCGGTCAAGACGACCCAAATCGGCGGACCGCGCAGCTACGATGGCGGCAAGAAGGTCACAG
GCCGCAAGCGCCAGATCCTGGTCGACACCGAGGGCATTCTACTCAAGGTTGTGGTTCATTCCGCTGATCTCCACGATAAGGCGGGCGGGATGCTCCTGCT
CGCTTGGCTCCACCTCCTGTTCCCGCGGCTCGTTCTGGTGTGGTCCGACACCCATTATCAAGGCTTGAAGGCGTGGGCAAAAGGTCACCTCGGCTGGACG
ATCGAAGTCGTCAAGCATTGGTGGACCGGCGTTCGCGGCTTCTGGTGTGCACCCGGCCAGGAACCACCCGAAGTTCTCACTGGCTTTCACGTGTTGCCGC
GACGTTGGGTTGTTGAGCGATGCTTTTCTTGGTTGTTGACAAACCGCCGCTTCGTCGTCGATTACGAACGTCTACCGAAAACAGACGAAGCTTTTATTTA
TATGGCGATGGGGCGAATTTTACTCCGCCGCCTCGCAAAAGCCCTCCCAAATTAGCCAAAAACTCGATCCACCTTTTACCGGACAGCTTC
Protein section
ORF number : 1

 

ORF 1
LengthBeginEndStrandFusion ORF
846 bp281 aa110955+No
ORF function : Transposase
Chemistry : DDE

ORF sequence :

MPCPHDPYPSDLSDDEWEVVAPLFSKSERRGRKPIYDLRRILDGCFYVLRGGIAWRMMAHDLPPWRVVYDHFSQWRKTGKWERINEALRERYRVSRGRDP
QPSAAVIDSQSVKTTQIGGPRSYDGGKKVTGRKRQILVDTEGILLKVVVHSADLHDKAGGMLLLAWLHLLFPRLVLVWSDTHYQGLKAWAKGHLGWTIEV
VKHWWTGVRGFWCAPGQEPPEVLTGFHVLPRRWVVERCFSWLLTNRRFVVDYERLPKTDEAFIYMAMGRILLRRLAKALPN

 

Blast result :
Comments
ISAzs16 is 64% aa similar to ISMac15.
References
1] ISfinder annotation (2010).
2] Kaneko,T., Minamisawa,K., Isawa,T., Nakatsukasa,H., Mitsui,H., Kawaharada,Y., Nakamura,Y., Watanabe,A., Kawashima,K., Ono,A., Shimizu,Y., Takahashi,C., Minami,C., Fujishiro,T., Kohara,M., Katoh,M., Nakazaki,N., Nakayama,S., Yamada,M., Tabata,S. and Sato,S. (2010) DNA Res. 17 (1), 37-50.