ISfinder Mirror Site

ISAzs15

Family ISAs1
Group

MGE type ISRelated element(s) :
Isoform Synonym(s) ISazl24_01

Accession number	Transposition	Origin	Host
NC_013854	ND	Azospirillum sp.	Azospirillum sp. B510 plasmid pAB510a Azospirillum sp. B510 Azospirillum sp. B510 plasmid pAB510c

DNA section

IS Length : 1263 bp

Ends

IR Length : 16

IRL : CAGGGCAATCGCTTGAAGCGGCGCTTGACGCATCGAGTTTAAACGCGGTT
IRR : CAGGGCAATCGCTTGACCGACGTTAGCCGCGCTGCCGGATGACGGTTTCG

Insertion site

Left flank	Direct repeat	Right flank	DR Length
AAGTCCGACC	GAGTCAAGT	CCGACCGTTC	9
AAGCGTCCCC	GCCGGTCACG	GCGGCGTTCC	10
CCAGTACCGG	CTTCCGCAGG	GCAGACCATG	10
CAAATCCAAT	ACGTTCCTGA	CGCGGGCATC	10
AGCATCGGGC	GGCGGGAA	AGGGGAAGGC	8

DNA sequence

CAGGGCAATCGCTTGAAGCGGCGCTTGACGCATCGAGTTTAAACGCGGTTGTGGATTCCCTCTGAAGGGCGGGCATGATTCATAGGAGCCTCCATTGATG
GGAGGCAGGTATGGCGGAAGGGGCTGGCAAGTCGCTGTTGGATCACTTCTCGGCGCTGGAGGATCCGCGTCAGGCATGGAAGGTCGTATATCCGCTGCCG
GAAATCCTGCTCCTGGTGCTGTGTGCCACCCTGGGTGGGGCGGAGAACTTTGTCGAGATCGAGGAGTGGGGCGAGGATCGCCTGGACTTTCTGCGTCGTT
TCCTGCCCTACCGGCGAGGCATCGCCAGCCATGACACGCTCAACGACGTGATGAACGCGCTTGATGGCGAGCTGTTTTCGTCCTGCTTCACGGCGTGGGT
GGACGGGTTGCGCGAGGGCGAGCCGGACATCGTCGCCATTGACGGCAAGACCTCCCGGCGCGCTCACGCTCGGGCTCAGGGGCGCAACCCGCTGCATCTC
GTCTCCGCCTGGGCCAGCCGACAGCGGCTGGTGCTGGGCCAGCAAGCCTGCGAGGCGAAGTCGAACGAGATCACCGCCATTCCACTGCTGCTGGAGCGCT
TGGCCCTGACCGGAGCATTGGTAACCATCGATGCCATGGGGTGCCAGACCAAGATCGCCCAAGCCATTCTCGACAAGGGTGCCGACTATCTGCTGGCGGT
GAAGGGCAACTGGCCGATCCTGTGCGGGGAAATCGAGCGCTACTTCAGCGAGGCACGCGACGGCGTATCCGACACGTTCACCACCACCGACGGCGACCAT
GGCCGGATCGAAGTCCGCCACCATGTCGTCAGCCACGACGTCGACTGGCTGTCCACCGACCGCCGCTTCCCGGGCGAGCCCCGCTTCCCCGCCTTGACCA
GCATCGCCATGGTCGAGGCCGACGTCGAGCGGGAGGGTAAGCCCAGCCGAGAACGGCGTTACTTTCTCTCCTCGGCACGCCTCGACGCCCGTCTCCTGGC
CCACGCTGTCCGCTGCCATTGGCATGTCGAGAACCGCCTGCACTGGGTGCTTGATGTCGTCTTCCACGACGACCTCTCCCGCTTGCGGTCCGGCTTCGGC
CCTCAAAACATGGCCGCCATCCGGCACATGGCCATAAACCTCATCCGAAAGGCCCCAGGCAAACAGAGCCTGACCGTCAAGCGTAAGAAAATGTCCTGGA
ACGCCGACTACCTCGAAACCGTCATCCGGCAGCGCGGCTAACGTCGGTCAAGCGATTGCCCTG

Protein section

ORF number : 1

ORF 1

Length		Begin	End	Strand	Fusion ORF
1131 bp	376 aa	111	1241	+	No

ORF function : Transposase
Chemistry : Unknow

ORF sequence :

MAEGAGKSLLDHFSALEDPRQAWKVVYPLPEILLLVLCATLGGAENFVEIEEWGEDRLDFLRRFLPYRRGIASHDTLNDVMNALDGELFSSCFTAWVDGL
REGEPDIVAIDGKTSRRAHARAQGRNPLHLVSAWASRQRLVLGQQACEAKSNEITAIPLLLERLALTGALVTIDAMGCQTKIAQAILDKGADYLLAVKGN
WPILCGEIERYFSEARDGVSDTFTTTDGDHGRIEVRHHVVSHDVDWLSTDRRFPGEPRFPALTSIAMVEADVEREGKPSRERRYFLSSARLDARLLAHAV
RCHWHVENRLHWVLDVVFHDDLSRLRSGFGPQNMAAIRHMAINLIRKAPGKQSLTVKRKKMSWNADYLETVIRQRG

Blast result :

Comments

ISAzs15 is 65% aa similar to ISAli14.

References

1] ISfinder annotation (2010).
2] Kaneko,T., Minamisawa,K., Isawa,T., Nakatsukasa,H., Mitsui,H., Kawaharada,Y., Nakamura,Y., Watanabe,A., Kawashima,K., Ono,A., Shimizu,Y., Takahashi,C., Minami,C., Fujishiro,T., Kohara,M., Katoh,M., Nakazaki,N., Nakayama,S., Yamada,M., Tabata,S. and Sato,S. (2010) DNA Res. 17 (1), 37-50.