ISfinder Mirror Site

ISAzs14

Family IS630
Group

MGE type ISRelated element(s) :
Isoform Synonym(s)

Accession number	Transposition	Origin	Host
NC_013854	ND	Azospirillum sp.	Azospirillum sp. B510 plasmid pAB510f Azospirillum sp. B510 plasmid pAB510a Azospirillum sp. B510 Azospirillum sp. B510 plasmid pAB510e

DNA section

IS Length : 1176 bp

Ends

IR Length : 18/23

IRL : TACCAGAGCCGGAAACTTCTGACTCGCCGGGTCTGTGGCTTGCGGTTCAG
IRR : TACCAAAGCCGTTAAATTTTGACCTGTAAGGCCCACGGGTAACCGCATGT

Insertion site

Left flank	Direct repeat	Right flank	DR Length
GTTCGCCAAGTAA		TGACCACCGGCGA	0
GGCATGCATCTAA		TCACTCCTCCAGC	0
TGCGACTCAA	TGA	GATCTGCCAG	3
CGGATGGTCC	TAA	CCGTGTTCGC	3
GCCGACCCAG	TCA	GCCAGCAGCG	3
GACGTGCCGA	TGA	CACCGGTCTC	3
GCACGGTGTC	TCA	CTCTCATTGG	3
CGACCGCCTG	TCA	CACGCCGTTG	3
GACGCATCCT	TTA	CACCACAAAT	3

DNA sequence

TACCAGAGCCGGAAACTTCTGACTCGCCGGGTCTGTGGCTTGCGGTTCAGCGCCGGTAGTGATTCCCTGTGCCAGGTCTCACCATTGAGCGAAGCACAGC
CATGTCCGCCCTGCCGATCCGCCCCGACCTGAGCTCGGAAGACCTGCGCCGCCTCGCCCGCGGCGAGAGCGATGGGCGGGTGTGCCGGCGCCTGCTGGCG
ATCGCCATGGCGCTGGACGGCGTCAGCCGGGCGGAAGCGGCGCGGCAAGCCGGCATGGACCGGCAAGCGCTGCGCGACTGGGTCGTGCGCTACAACGCCG
AAGGGGTGGACGGCTTGCGGGATCGGGCGCGCTGCGGCCGGCCGCCGCTGCTCGCCGACGCCCTGGAGCCGGAATTGGCTGACCTGATCGCCGCCGGCCC
CGAGGTCGAACGGGACGGCGTCGTCGAGTACCGGGTTCGCCACATCCGCGACTTGGCCCTGCGGCATTTCGGGGCGGACTACAGCCGCACCGGCATGCAG
GACCGCTTGCACCGCATGAAGCTGTCCTTCCTGACGCCGCGCCCGATCCATCCCAAGGCCGACGCACCGGCCCAGGAGGCGTTTAAAAAAACTTCGCCGA
ACGCCTCGCCGCCATCGGAGAGGACCACCCCGAGGCGAGCGCCGTGGAGGTCTGGTTCCAGGACGAGGCCCGCATCGGCCAGAAAGGCACCCTGACCCGG
CGCTGGGCGCCGCGCGGCAGCCGGCCGCGCGCCGTGCGCGATCATCGTTTCAAGTCGGCCTATCTCTTCGGCGCGGTCTGTCCTGAGCGCGACACCGGAG
CCGCCATCGTCATGACGCGCGCCAACACCGAGGCGATGAACCTCATGCTGGAGGAGATCAGCCGCACCGTCACCCCCGGCGCCCATGCCGCCGTGGTGAT
CGACGGGGCGGGATGGCACACCAGCGGCGATCTCGCCGTGCCGGCCAACATCACCCTCGTGCCGCTCCCGCCCTACAGCCCGGAACTCAACGCCATCGAA
AGGCTCTGGCAGGTCATGCGCGACACCCTGCTGTCCCACCGGCTCTTCACCGACCTCAACGCCATCCTCGACGTCTGCTGTCGCGTCTGGAACCGCATCC
TCGCCGAACCCGGCCGCATCCGCTCCACATGCGGTTACCCGTGGGCCTTACAGGTCAAAATTTAACGGCTTTGGTA

Recoding section

Recoding by frameshift
Frame
Type
Experimentally demonstrated

Stimulators :

Shine-Dalgarno sequence :
Secondary structure :

Recoding motif :

Protein section

ORF number : 3

ORF 1

Length		Begin	End	Strand	Fusion ORF
627 bp	208 aa	69	695	+	No

ORF function : Transposase
Description : First part of the transposase

ORF sequence :

Blast result :

ORF 2

Length		Begin	End	Strand	Fusion ORF
579 bp	192 aa	587	1165	+	No

ORF function : Transposase
Description : Second part of the transposase

ORF sequence :

KNFAERLAAIGEDHPEASAMEVWFQDEARIGQKGTLTRRWAPRGSRPRAVRDHRFKSAYLFGAVCPERDTGAAIVMTRANTEAMNLMLEEISRTVTPGAH
AAVVIDGAGWHTSGDLAVPANITLVPLPPYSPELNAIERLWQVMRDTLLSHRLFTDLNAILDVCCRVWNRILAEPGRIRSTCGYPWALQVKI

Blast result :

ORF 3

Length		Begin	End	Strand	Fusion ORF
1097 bp	365 aa	69	1165	+	Yes

ORF function : Transposase
Chemistry : DDE

ORF sequence :

MPGLTIERSTAMSALPIRPDLSSEDLRRLARGESDGRVCRRLLAIAMALDGVSRAEAARQAGMDRQALRDWVVRYNAEGVDGLRDRARCGRPPLLADALE
PELADLIAAGPEVERDGVVEYRVRHIRDLALRHFGADYSRTGMQDRLHRMKLSFLTPRPIHPKADAPAQEAFKKNFAERLAAIGEDHPEASAMEVWFQDE
ARIGQKGTLTRRWAPRGSRPRAVRDHRFKSAYLFGAVCPERDTGAAIVMTRANTEAMNLMLEEISRTVTPGAHAAVVIDGAGWHTSGDLAVPANITLVPL
PPYSPELNAIERLWQVMRDTLLSHRLFTDLNAILDVCCRVWNRILAEPGRIRSTCGYPWALQVKI

Blast result :

Comments

ISAzs14 is 80% aa similar to ISAli2. The third ORF is the putative ORFAB transposase reconstructed in silico by possible -1 frameshift.

References

1] ISfinder annotation (2010).
2] Kaneko,T., Minamisawa,K., Isawa,T., Nakatsukasa,H., Mitsui,H., Kawaharada,Y., Nakamura,Y., Watanabe,A., Kawashima,K., Ono,A., Shimizu,Y., Takahashi,C., Minami,C., Fujishiro,T., Kohara,M., Katoh,M., Nakazaki,N., Nakayama,S., Yamada,M., Tabata,S. and Sato,S. (2010) DNA Res. 17 (1), 37-50.