ISAzs11

  • Family ISAs1
  • Group
MGE type ISRelated element(s) :
Isoform Synonym(s) ISazl23_01
Accession numberTranspositionOriginHost
NC_013854 ND Azospirillum sp.
Azospirillum sp. B510 plasmid pAB510b
Azospirillum sp. B510 plasmid pAB510f
Azospirillum sp. B510 plasmid pAB510a
Azospirillum sp. B510
Azospirillum sp. B510 plasmid pAB510c
DNA section
IS Length : 1224 bp

Ends


IR Length : 16/18

IRL : CAGGGGAATCGCATTTGGAAAAATCGAGTAGCGTGTGTGAGAGAAAAGGA
IRR : CAGGGTCATCGCATTTGGCCGAGGATGGATCTTGCGAACTCGTCGGACCA

Insertion site


Left flankDirect repeatRight flankDR Length
CGGACGCATCTGGCCTGACGCCAGCGCCGT10
TCTTCCCAGGCAGAAATGCTTGTGCCTCAT10
GGGAAGCCCTGCCTTGCCGCCGCCGCGGCT10
TCGGAGCCCTGGATCTGGCCCATCAGCGACATTGACCGCT0
GATTCCCCTGGCCCTGGGTGCAGGGCGATT10
CGACCTGACCGCTGCGGCGGCCCGACGCCT10

DNA sequence

CAGGGGAATCGCATTTGGAAAAATCGAGTAGCGTGTGTGAGAGAAAAGGATTCTGTAAGGGGCTTTATCGCCGATTTTGGAGCCCCCGATGCAATCGTGG
TTTGAGAAAGCGTTCGATGCGTTGCCGGACCCACGCACGGGCAACGCCAAGCGGCACGACCTGCTGGAGATCCTGACGATCGCGCTGACGGCAACGGTGT
GCGGGGCGGAGAGCTGTGCCGACTTTGCCGACTTCGCGGTGGATCGCGAGGGGCTGTTTCGGTCGTTTTTGCGTCTGGAGAACGGGGTGCCGAGTCACGA
CACCTTCTCGCGCATCTTCCGGCTGCTGGACCCGGTGGCCTTTGCCGCCTGCTTTGGCCGTTTCCTGGAGGGGCTGGGAACGGCCGGGGCTGGGGTGATC
GCCATCGACGGCAAGACGTTACGCCGCTCCTTCGATGACGCGGCCCGGTCCAACCCGCTGGCGGTGGTGACGGCCTTCGCCTCGGCGACGCGGCTGGTGG
TGGGCCAGCACAGCTACCGGGTGGCCGAGGGCGACAGCGAGATCCTGGCCGCCCGTGCCCTGCTGGAGTGCCTGGATCTCCAGGGCCATCTGGTCACCGC
CGACGCCATCCATTGTCAGGAGGAGACCGCCCGCCTCATCCGGAAGCGCGGCGGCGACTACCTGCTGCGGCTGAAGGGCAACCATCCCGCCCTGCTCGCC
ATGGTCGAGGAGTACTTCACCGACCCGGAACTGTGCGCCGGGCTGGCGAGTGCCACGACCACCGACGCCGATCACGGCCGGATCGAGACCCGGCGGGCCT
GGGTGTGTCACGAATTGGATTGGCTGCGCGGTCCCAAAGCGGCCAGCAGCGAACCGGTCCTGCTGCCCGACATGGCCTTTCTGGGCTTGATCGAGGCTAC
CGTCGAGCATCGCGGCAAGACCACCGTGACCCGGCACTACCACATCGCCTCGCGCGCGCTCAGCGCGGCCGACTATCTGGAGGCCGCCCGCGCCCATTGG
TCGATCGAGAACGGGTTGCACTGGATGCTCGACGTCGTCTTCGACGAGGATCGCGCCCGCAACCGCAAGGATCACGGGCCGGAAAACCTCGCCACCCTCC
GCAAGCTCGCGCTCAACATCCTGAACCGCGCCCGTCGCGATATCTCCGTCCGCCGAAAGCGAAAGCGCTCAGGATGGTCCGACGAGTTCGCAAGATCCAT
CCTCGGCCAAATGCGATGACCCTG
Protein section
ORF number : 1

 

ORF 1
LengthBeginEndStrandFusion ORF
1131 bp376 aa891219+No
ORF function : Transposase
Chemistry : Unknow

ORF sequence :

MQSWFEKAFDALPDPRTGNAKRHDLLEILTIALTATVCGAESCADFADFAVDREGLFRSFLRLENGVPSHDTFSRIFRLLDPVAFAACFGRFLEGLGTAG
AGVIAIDGKTLRRSFDDAARSNPLAVVTAFASATRLVVGQHSYRVAEGDSEILAARALLECLDLQGHLVTADAIHCQEETARLIRKRGGDYLLRLKGNHP
ALLAMVEEYFTDPELCAGLASATTTDADHGRIETRRAWVCHELDWLRGPKAASSEPVLLPDMAFLGLIEATVEHRGKTTVTRHYHIASRALSAADYLEAA
RAHWSIENGLHWMLDVVFDEDRARNRKDHGPENLATLRKLALNILNRARRDISVRRKRKRSGWSDEFARSILGQMR

 

Blast result :
Comments
ISAzs11 is 56% aa similar to ISRpa5.
References
1] ISfinder annotation (2010).
2] Kaneko,T., Minamisawa,K., Isawa,T., Nakatsukasa,H., Mitsui,H., Kawaharada,Y., Nakamura,Y., Watanabe,A., Kawashima,K., Ono,A., Shimizu,Y., Takahashi,C., Minami,C., Fujishiro,T., Kohara,M., Katoh,M., Nakazaki,N., Nakayama,S., Yamada,M., Tabata,S. and Sato,S. (2010) DNA Res. 17 (1), 37-50.