ISfinder Mirror Site

ISAzs10

Family IS5
Group

MGE type ISRelated element(s) :
Isoform Synonym(s)

Accession number	Transposition	Origin	Host
NC_013854	ND	Azospirillum sp.	Azospirillum sp. B510 plasmid pAB510f Azospirillum sp. B510 plasmid pAB510a Azospirillum sp. B510

DNA section

IS Length : 1307 bp

Ends

IR Length : 24/29

IRL : CAGGCCGCGGAAAAAGGGCTGTGCACGGCTGTCGTGTCGTGATTCCCTCC
IRR : CAGGACGCGGAAAAAGGCTCGTGCATGGCGTCCTTGCCTCTGCGGAGGCT

Insertion site

Left flank	Direct repeat	Right flank	DR Length
ATCTGAGCCG	CTAA	GGCGGTCTCG	4
ATATCGGGCT	TTAA	GCTTCGCGAC	4
GCCACCGCGC	TTAG	AAGGAAAACG	4
CCTAAACACCCTAA		TTAACACCAACAGC	0
GAGTTATGAA	TTAG	GCTTGGATGC	4
CACCTGATCCTTAG		TTAAACCAAAGCGT	0
GAGTTATGAA	TTAG	GGCGGCAGCG	4
CCTCGGTCTT	TTAG	AGGCCGTTGA	4
GATGGCCCAG	TTAG	AGCGGCAGCC	4

DNA sequence

CAGGCCGCGGAAAAAGGGCTGTGCACGGCTGTCGTGTCGTGATTCCCTCCAGTGAGGGCAAAGGCGACCGTTGAGGGAATGATGCGGGGCTCGGACGAAC
GCAGCGAGGGTCTTTTCAGCTACGTGAGCTGCGAGGCACGGGTTCCGGCGACCCATCCATTGCGGCCGATCCGGGCCATCGTGGACGAAGCGCTGGAGGT
GATGTCACCGGCGTTCGAGGGGCTGTACTCCAAGATCGGCCGGCCGTCGATCCCGCCGGAAAAGCTGCTGCGGGCGCTGCTGCTCCAGGCCTTCTACTCG
GTGCGCTCGGAACGCCAGCTGATGGAACAGCTCGACTATAACCTGCTGTTCCGCTGGTTCGTCGGCTTGTCGATGGACGCTCCGGTGTGGGACGTGACGG
TGTTCACCAAGAACCGGGAGCGTCTGCTGGCCGGGGATGTGGCGGCCAAGTTCCTGGCCACCGTGCTGGGCCAGCCGAAGGTGAAAGCGCTGCTGTCGGA
CGAGCACTTCTCGGTGGACGGGACGCTGATCGAAGCCTGGGCCAGCGTGAAGAGCTTCCGGCCCAAAGATGGCAGCGGTGAGCCGCCGGGGCCGGGACGC
AACGGCGAACGCGACTTTCACGGCGAGAAGCGGTCCAACGAGACCCATGCCTCGACCAGCGACCCCGAGGCGCGGCTGTACCGCAAGGGCAACGGGCAGC
CGGCGAAGCTGGCCTTCATGGGTCATGCGTTGATGGAGAACCGGAACGCCCTGGTGGTCAATGTCCGGCTCACCGCGGCGACCGGCTTGGCCGAGCGCGA
GGCGGCGGTGTCCATGGTCGAGGCCATCCCCGGCCGCCACCGCATCACGGTGGGCGCCGACAAGGCCTACGACACCAAGGATTTCGTGGCGAACATGCGC
GAGTTGGGCGCCGCCCCGCACGTCGCCCAGAACACCCGCAACCGCCGCTCGGCCATCGACGGCCGAACCACCCGCCACCCCGGCTATGCAGTCAGCCTAC
GAATCCGCAAGCGCATCGAGGAGGTGTTCGGCTGGATCAAAGGGGCCGGGCTACGCAAGACCCGCCATCGCGGCACGGCCCGTGTCGGCTGGATGTTCAC
CCTGACCGCCGCCGCCTACAACCTGATCCGCCTGCCAAAGCTACTGGCGGCGGCATAATCACGCCCGGAGTCCGCCCAAAAGGCGGCCTTGGGCGCTCCA
GGCCGGGGGTGCACCCGCTGATTTCGACCAAAGCGGGTGCAAAAGGCCCAAATTTCGAGCCTCCGCAGAGGCAAGGACGCCATGCACGAGCCTTTTTCCG
CGTCCTG

Protein section

ORF number : 1

ORF 1

Length		Begin	End	Strand	Fusion ORF
1077 bp	358 aa	82	1158	+	No

ORF function : Transposase
Chemistry : DDE

ORF sequence :

MRGSDERSEGLFSYVSCEARVPATHPLRPIRAIVDEALEVMSPAFEGLYSKIGRPSIPPEKLLRALLLQAFYSVRSERQLMEQLDYNLLFRWFVGLSMDA
PVWDVTVFTKNRERLLAGDVAAKFLATVLGQPKVKALLSDEHFSVDGTLIEAWASVKSFRPKDGSGEPPGPGRNGERDFHGEKRSNETHASTSDPEARLY
RKGNGQPAKLAFMGHALMENRNALVVNVRLTAATGLAEREAAVSMVEAIPGRHRITVGADKAYDTKDFVANMRELGAAPHVAQNTRNRRSAIDGRTTRHP
GYAVSLRIRKRIEEVFGWIKGAGLRKTRHRGTARVGWMFTLTAAAYNLIRLPKLLAAA

Blast result :

Comments

ISAzs10 is 79% aa similar to ISSpa12.

References

1] ISfinder annotation (2010).
2] Kaneko,T., Minamisawa,K., Isawa,T., Nakatsukasa,H., Mitsui,H., Kawaharada,Y., Nakamura,Y., Watanabe,A., Kawashima,K., Ono,A., Shimizu,Y., Takahashi,C., Minami,C., Fujishiro,T., Kohara,M., Katoh,M., Nakazaki,N., Nakayama,S., Yamada,M., Tabata,S. and Sato,S. (2010) DNA Res. 17 (1), 37-50.