ISAzs9
- Family IS5
- Group IS427
Isoform Synonym(s)
| Accession number | Transposition | Origin | Host |
|---|---|---|---|
| NC_013854 | ND | Azospirillum sp. | Azospirillum sp. B510 plasmid pAB510b Azospirillum sp. B510 plasmid pAB510a Azospirillum sp. B510 Azospirillum sp. B510 plasmid pAB510d Azospirillum sp. B510 plasmid pAB510c |
DNA section
IS Length : 845 bp
Ends
IR Length : 15
IRL : GGCCTGTTGACATTCGCGTTGAGAAGAGGCTTCCGGTGGTCGTAGAAATT
IRR : GGCCTGTTGACATTCATCGTGTTACCAAGACGGTAGCGACGAGGTTAATG
Insertion site
| Left flank | Direct repeat | Right flank | DR Length |
|---|---|---|---|
| CTGGCGGGGGCTAG | CTAAGAGTTTCAGA | 0 | |
| CGGCTTGGCA | CTAG | TACTACAGCC | 4 |
| GCATACGATG | CTAG | GGCGGATAAT | 4 |
| ACCAAGTCGG | CTAG | TCCCCTCAAG | 4 |
| GCCGGCCGAT | CTAG | CCCGGCTGGT | 4 |
| AAATTGGCTTTTAG | CTAGTACTACAGCC | 0 | |
| TTCGAAAAAA | CTAG | TACTACAGCC | 4 |
| GGCTGTAGTA | CTAG | AAAAAAATGC | 4 |
| CGGTGCGGCTGTAG | CTAGAAAAAAATTC | 0 | |
| GGCTGTAGTA | CTAG | TTCTACTATG | 4 |
| AAATTTTTTT | CTAG | TACTACAGCC | 4 |
| CGCAGAGTGG | CTAG | GTTACTGACA | 4 |
| GGAGAGGGAGCTAG | CTAATACTACAGCC | 0 |
DNA sequence
GGCCTGTTGACATTCGCGTTGAGAAGAGGCTTCCGGTGGTCGTAGAAATTTGATTCAAGGCTACCAAAAGGGAGGTGGCCTTGGATCGGATTGTATTGCG
AGATGATCAGTGGGAGCGGATAGCGCCGCTTCTTCCCGGCAAAGTGGGCGACCCTGGCCGCTCGGCAGCGGACAATCGCCTGTTTTTGGAGGCGGTCCTG
TGGATCGTCCGCGTTGGAGCGCCCTGGCGGGACTTGCCCGCAGCGTTCGGCAATTGGAACTCGGTGTTCCAGCGCTATCGCCGATGGGCCAAGGCCGGTG
TCTTCGACGAGGTCTTCGCCGCTTTATCGAGCGACGCGGACTTCGAGTACGCGATCATTGATGGCACGATTGTCCGGGTGCATCAGCACGGTACCGGCGC
AAGGGGGGGACTCAGGCTCAGGCCATCGGTCGCTCTCGTGGCGGGCTGACGACCAAGATCCTGGCCGTCGTCGATGCGTTGGGGAACCTTGGGCGCTTCA
TCCTGCTGCCGGGCCAGCGACATGACAGCGTCGGCGTCGAACCGGTGCTGGACGGTATCGAATTCTCCGCCTTGCTGGCTGACAAAGCCTTCGACAGCAA
CGCCATCCGTATCTTGATCGCCGAGCGAGGCGCGGTCGCCGTCATTCCGTCCAAGGCCAACCGATCTCCCCCGATTCCCCACGACGCCGAGATGTACAAA
TGGCGCCATCTGGTCGAGAACTTCTTCTGCAAAATCAAAGAATTCCGACGCATCGCCACACGATACGACAAAACTGATACAAGCTTCGCCGCTGCCATTA
ACCTCGTCGCTACCGTCTTGGTAACACGATGAATGTCAACAGGCC
AGATGATCAGTGGGAGCGGATAGCGCCGCTTCTTCCCGGCAAAGTGGGCGACCCTGGCCGCTCGGCAGCGGACAATCGCCTGTTTTTGGAGGCGGTCCTG
TGGATCGTCCGCGTTGGAGCGCCCTGGCGGGACTTGCCCGCAGCGTTCGGCAATTGGAACTCGGTGTTCCAGCGCTATCGCCGATGGGCCAAGGCCGGTG
TCTTCGACGAGGTCTTCGCCGCTTTATCGAGCGACGCGGACTTCGAGTACGCGATCATTGATGGCACGATTGTCCGGGTGCATCAGCACGGTACCGGCGC
AAGGGGGGGACTCAGGCTCAGGCCATCGGTCGCTCTCGTGGCGGGCTGACGACCAAGATCCTGGCCGTCGTCGATGCGTTGGGGAACCTTGGGCGCTTCA
TCCTGCTGCCGGGCCAGCGACATGACAGCGTCGGCGTCGAACCGGTGCTGGACGGTATCGAATTCTCCGCCTTGCTGGCTGACAAAGCCTTCGACAGCAA
CGCCATCCGTATCTTGATCGCCGAGCGAGGCGCGGTCGCCGTCATTCCGTCCAAGGCCAACCGATCTCCCCCGATTCCCCACGACGCCGAGATGTACAAA
TGGCGCCATCTGGTCGAGAACTTCTTCTGCAAAATCAAAGAATTCCGACGCATCGCCACACGATACGACAAAACTGATACAAGCTTCGCCGCTGCCATTA
ACCTCGTCGCTACCGTCTTGGTAACACGATGAATGTCAACAGGCC
Recoding section
- Recoding by frameshift
- Frame
- Type
- Experimentally demonstrated
Stimulators :
- Shine-Dalgarno sequence :
- Secondary structure :
Recoding motif :
Protein section
ORF number : 3
ORF 1
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 375 bp | 124 aa | 75 | 449 | + | No |
Description : First part of the transposase
ORF sequence :
MALDRIVLRDDQWERIAPLLPGKVGDPGRSAADNRLFLEAVLWIVRVGAPWRDLPAAFGNWNSVFQRYRRWAKAGVFDEVFAALSSDADFEYAIIDGTIV
RVHQHGTGARGGLRLRPSVALVAG
RVHQHGTGARGGLRLRPSVALVAG
Blast result :ORF 2
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 472 bp | 156 aa | 361 | 832 | + | No |
Description : Second part of the transposase
ORF sequence :
WHDCPGASARYRRKGGTQAQAIGRSRGGLTTKILAVVDALGNLGRFILLPGQRHDSVGVEPVLDGIEFSALLADKAFDSNAIRILIAERGAVAVIPSKAN
RSPPIPHDAEMYKWRHLVENFFCKIKEFRRIATRYDKTDTSFAAAINLVATVLVTR
RSPPIPHDAEMYKWRHLVENFFCKIKEFRRIATRYDKTDTSFAAAINLVATVLVTR
Blast result :ORF 3
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 758 bp | 252 aa | 75 | 832 | + | Yes |
Chemistry : DDE
ORF sequence :
MALDRIVLRDDQWERIAPLLPGKVGDPGRSAADNRLFLEAVLWIVRVGAPWRDLPAAFGNWNSVFQRYRRWAKAGVFDEVFAALSSDADFEYAIIDGTIV
RVHQHGTGARGGTQAQAIGRSRGGLTTKILAVVDALGNLGRFILLPGQRHDSVGVEPVLDGIEFSALLADKAFDSNAIRILIAERGAVAVIPSKANRSPP
IPHDAEMYKWRHLVENFFCKIKEFRRIATRYDKTDTSFAAAINLVATVLVTR
RVHQHGTGARGGTQAQAIGRSRGGLTTKILAVVDALGNLGRFILLPGQRHDSVGVEPVLDGIEFSALLADKAFDSNAIRILIAERGAVAVIPSKANRSPP
IPHDAEMYKWRHLVENFFCKIKEFRRIATRYDKTDTSFAAAINLVATVLVTR
Blast result :
Comments
ISAzs9 is 96% aa similar to ISAli9. The third ORF is the putative ORFAB transposase reconstructed in silico by possible -1 frameshift.
References
1] ISfinder annotation (2010).
2] Kaneko,T., Minamisawa,K., Isawa,T., Nakatsukasa,H., Mitsui,H., Kawaharada,Y., Nakamura,Y., Watanabe,A., Kawashima,K., Ono,A., Shimizu,Y., Takahashi,C., Minami,C., Fujishiro,T., Kohara,M., Katoh,M., Nakazaki,N., Nakayama,S., Yamada,M., Tabata,S. and Sato,S. (2010) DNA Res. 17 (1), 37-50.
2] Kaneko,T., Minamisawa,K., Isawa,T., Nakatsukasa,H., Mitsui,H., Kawaharada,Y., Nakamura,Y., Watanabe,A., Kawashima,K., Ono,A., Shimizu,Y., Takahashi,C., Minami,C., Fujishiro,T., Kohara,M., Katoh,M., Nakazaki,N., Nakayama,S., Yamada,M., Tabata,S. and Sato,S. (2010) DNA Res. 17 (1), 37-50.