ISAzs7
- Family IS66
- Group ISBst12
Isoform Synonym(s)
| Accession number | Transposition | Origin | Host |
|---|---|---|---|
| NC_013854 | ND | Azospirillum sp. | Azospirillum sp. B510 |
DNA section
IS Length : 1455 bp
Ends
IR Length : 18/21
IRL : GTAACTGCTCACGGGGCTGGCGAAATGGGTTGCGCGGGGTGCTGGAATAT
IRR : GTAATTGCTCAGGTGGCTGGCAGCGCGGGTGCTTCGGCGACAGCGAAGGT
Insertion site
| Left flank | Direct repeat | Right flank | DR Length |
|---|---|---|---|
| GGGTCAGGCT | CTAAGGAC | GGCGGCTTTA | 8 |
DNA sequence
GTAACTGCTCACGGGGCTGGCGAAATGGGTTGCGCGGGGTGCTGGAATATGAGTCAACGTCGGGATGGAGAAACCGCCGCGCCATCGCCTGAGTGATGCC
GAGAAGGACGCCCTGCTTTTGGAGCAGGCGGCGTTGATCGAGCGTCTGGCCGCGCGGATCACGGAACTCGAGGCGCTGGTCGGCAAGCCGCGCAAGACGT
CGTCGAATTCCCACACGCCGCCTTCACAGGACGGCCCCGGCCGCCCCGGCTCGAAGGCGGCGGCCACGCGCCGGCGCAAGCCGCGACCATCGCGTCCCGG
CGTGTCGCGCCCGCTGAGCGAGGAGCCGGACAAGACGGATCGGCGTCTGGCCGAGAGCTGCCCCCATTGCGGGACGGCGGTATCGGCGATGCCCCAGCGC
TGCCGACACCGCTACGATCACATCGATCTTCCGGTCATCCGCCCGATGGTGACACGGGTGGAACTGTTCGGTGGTCGCTGTGGCGATTGCGGGCGGCGCT
ACCGGGCGGAGCCTCCGGCTTTGATGCCGCCGGGCACACCGTTCGGCCCCGGCATCCGGGCGTTGCTGGCCTACCTTCACCACAGCCACCACGTCGGCTT
CGAGCGTCTGTCGCGGATGCTGAAAGAGGTGTTCGGGCTGACCATCTCAGAAGGCGCCATCGCCAACAGCTTCCGGCGGATGGGGGGGGCGTTCGACACG
GCCCGCGCGGCGATCAAGGCCAAGCTGTTGACCGCGCCGGTCATCGCGTCCGACGAAACCACCACGCGGGTGAATGGCGTCACCCAGTGGCAGTGGGTCT
TCCACTCCGACAAGGCGGTGCTGCACGACATCGTCCCCAGCCGGGCCCGGAGCGTCGCGGCGCAGCTTCTCGGTGACCATCGGCCCGAGGTCTGGGTCTC
CGACCGCTATGCCGGGCAGCAGGAATTGGGGCGCGTCCATCAGGTCTGCCTGGCCCATGTCCTGCGCGATGTCCAGTACGCCATCGACTGCGGCGACACC
GTCGTCGCTCCCAAAATCCGCGACCATCTGCGCTGGGCCATCCGCATCGGCAAGCGGCGCCCCGCCTCGAAGGACAGCACGCTCGCCGCTTACGCCGCCA
GAGCCGATCGGGGCCTTGATGTCCTGCTCGGCGTTCCCGCCGCCCATCCCGCCGGACGCGACCTGCAGCGCCAGATCAAGGCGTGGCGCGGCAAGTTCTT
CGTCTTCCTCACCGACCGCCGCGTCCCGCCAACCAACAACGGCAGCGAGCGGGAAATCCGGCCCTCCGTCATCTTCCGCAAGGTCACCAACGGCTTTCGA
TCAACCTGGGGGCCGGGCATCCACGCCGGCTATCGATCCGTCACCGGGACTGCCCGCTTGCAGGGCCAGTCCGCCTGGACCGCCATCCGCGACCTCGTCG
ATGGCACCTTCGCTGTCGCCGAAGCACCCGCGCTGCCAGCCACCTGAGCAATTAC
GAGAAGGACGCCCTGCTTTTGGAGCAGGCGGCGTTGATCGAGCGTCTGGCCGCGCGGATCACGGAACTCGAGGCGCTGGTCGGCAAGCCGCGCAAGACGT
CGTCGAATTCCCACACGCCGCCTTCACAGGACGGCCCCGGCCGCCCCGGCTCGAAGGCGGCGGCCACGCGCCGGCGCAAGCCGCGACCATCGCGTCCCGG
CGTGTCGCGCCCGCTGAGCGAGGAGCCGGACAAGACGGATCGGCGTCTGGCCGAGAGCTGCCCCCATTGCGGGACGGCGGTATCGGCGATGCCCCAGCGC
TGCCGACACCGCTACGATCACATCGATCTTCCGGTCATCCGCCCGATGGTGACACGGGTGGAACTGTTCGGTGGTCGCTGTGGCGATTGCGGGCGGCGCT
ACCGGGCGGAGCCTCCGGCTTTGATGCCGCCGGGCACACCGTTCGGCCCCGGCATCCGGGCGTTGCTGGCCTACCTTCACCACAGCCACCACGTCGGCTT
CGAGCGTCTGTCGCGGATGCTGAAAGAGGTGTTCGGGCTGACCATCTCAGAAGGCGCCATCGCCAACAGCTTCCGGCGGATGGGGGGGGCGTTCGACACG
GCCCGCGCGGCGATCAAGGCCAAGCTGTTGACCGCGCCGGTCATCGCGTCCGACGAAACCACCACGCGGGTGAATGGCGTCACCCAGTGGCAGTGGGTCT
TCCACTCCGACAAGGCGGTGCTGCACGACATCGTCCCCAGCCGGGCCCGGAGCGTCGCGGCGCAGCTTCTCGGTGACCATCGGCCCGAGGTCTGGGTCTC
CGACCGCTATGCCGGGCAGCAGGAATTGGGGCGCGTCCATCAGGTCTGCCTGGCCCATGTCCTGCGCGATGTCCAGTACGCCATCGACTGCGGCGACACC
GTCGTCGCTCCCAAAATCCGCGACCATCTGCGCTGGGCCATCCGCATCGGCAAGCGGCGCCCCGCCTCGAAGGACAGCACGCTCGCCGCTTACGCCGCCA
GAGCCGATCGGGGCCTTGATGTCCTGCTCGGCGTTCCCGCCGCCCATCCCGCCGGACGCGACCTGCAGCGCCAGATCAAGGCGTGGCGCGGCAAGTTCTT
CGTCTTCCTCACCGACCGCCGCGTCCCGCCAACCAACAACGGCAGCGAGCGGGAAATCCGGCCCTCCGTCATCTTCCGCAAGGTCACCAACGGCTTTCGA
TCAACCTGGGGGCCGGGCATCCACGCCGGCTATCGATCCGTCACCGGGACTGCCCGCTTGCAGGGCCAGTCCGCCTGGACCGCCATCCGCGACCTCGTCG
ATGGCACCTTCGCTGTCGCCGAAGCACCCGCGCTGCCAGCCACCTGAGCAATTAC
Protein section
ORF number : 1
ORF 1
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 1383 bp | 460 aa | 65 | 1447 | + | No |
Chemistry : DDE
ORF sequence :
MEKPPRHRLSDAEKDALLLEQAALIERLAARITELEALVGKPRKTSSNSHTPPSQDGPGRPGSKAAATRRRKPRPSRPGVSRPLSEEPDKTDRRLAESCP
HCGTAVSAMPQRCRHRYDHIDLPVIRPMVTRVELFGGRCGDCGRRYRAEPPALMPPGTPFGPGIRALLAYLHHSHHVGFERLSRMLKEVFGLTISEGAIA
NSFRRMGGAFDTARAAIKAKLLTAPVIASDETTTRVNGVTQWQWVFHSDKAVLHDIVPSRARSVAAQLLGDHRPEVWVSDRYAGQQELGRVHQVCLAHVL
RDVQYAIDCGDTVVAPKIRDHLRWAIRIGKRRPASKDSTLAAYAARADRGLDVLLGVPAAHPAGRDLQRQIKAWRGKFFVFLTDRRVPPTNNGSEREIRP
SVIFRKVTNGFRSTWGPGIHAGYRSVTGTARLQGQSAWTAIRDLVDGTFAVAEAPALPAT
HCGTAVSAMPQRCRHRYDHIDLPVIRPMVTRVELFGGRCGDCGRRYRAEPPALMPPGTPFGPGIRALLAYLHHSHHVGFERLSRMLKEVFGLTISEGAIA
NSFRRMGGAFDTARAAIKAKLLTAPVIASDETTTRVNGVTQWQWVFHSDKAVLHDIVPSRARSVAAQLLGDHRPEVWVSDRYAGQQELGRVHQVCLAHVL
RDVQYAIDCGDTVVAPKIRDHLRWAIRIGKRRPASKDSTLAAYAARADRGLDVLLGVPAAHPAGRDLQRQIKAWRGKFFVFLTDRRVPPTNNGSEREIRP
SVIFRKVTNGFRSTWGPGIHAGYRSVTGTARLQGQSAWTAIRDLVDGTFAVAEAPALPAT
Blast result :
Comments
ISAzs7 is 85% aa similar to ISRhru2.
References
1] ISfinder annotation (2010).
2] Kaneko,T., Minamisawa,K., Isawa,T., Nakatsukasa,H., Mitsui,H., Kawaharada,Y., Nakamura,Y., Watanabe,A., Kawashima,K., Ono,A., Shimizu,Y., Takahashi,C., Minami,C., Fujishiro,T., Kohara,M., Katoh,M., Nakazaki,N., Nakayama,S., Yamada,M., Tabata,S. and Sato,S. (2010) DNA Res. 17 (1), 37-50.
2] Kaneko,T., Minamisawa,K., Isawa,T., Nakatsukasa,H., Mitsui,H., Kawaharada,Y., Nakamura,Y., Watanabe,A., Kawashima,K., Ono,A., Shimizu,Y., Takahashi,C., Minami,C., Fujishiro,T., Kohara,M., Katoh,M., Nakazaki,N., Nakayama,S., Yamada,M., Tabata,S. and Sato,S. (2010) DNA Res. 17 (1), 37-50.