ISAzs6

  • Family IS701
  • Group
MGE type ISRelated element(s) :
Isoform Synonym(s) ISazl19_01
Accession numberTranspositionOriginHost
NC_013854 ND Azospirillum sp.
Azospirillum sp. B510 plasmid pAB510b
Azospirillum sp. B510 plasmid pAB510a
Azospirillum sp. B510
Azospirillum sp. B510 plasmid pAB510d
Azospirillum sp. B510 plasmid pAB510c
DNA section
IS Length : 1385 bp

Ends


IR Length : 14

IRL : TACTACAGCCGGTCGCGAAAGACGGAATATGCGGCCGGGCGGCCTGTTGA
IRR : TACTACAGCCGGTCCTTATCGGTTTGGGTGCGTCGTTTCCAATGGGAGCG

Insertion site


Left flankDirect repeatRight flankDR Length
TCAACAGGCCCTAGGCGGCAACCC4
CGCTCGAGCCCTAGGGCCTGTTGA4
TCCGGTCTAGATAGCCGGCG0
TCAACAGGCCCTAGGGCCTGTTGA4
TATGAATTAGCTAGGGCCTG0
GCTTGCCGTCCTAGGGCCTGTTGA4
TTCACCGTGGCTAGGGCCTGTTGA4
GGGTGGCGGGTTAGGGCCTGTTGA4

DNA sequence

TACTACAGCCGGTCGCGAAAGACGGAATATGCGGCCGGGCGGCCTGTTGAGCTTGGAACCGACTGGAGGGTCCGAGCATGACCGAGATGACGGAAGCGGC
GGAATGGTCCGATGCCCTGGAAGGGCTGGTCGGCGGATTGAGGTCCTTCTACAGCGCGGCCGGTCGCCAACGGGCTCTGCGTTACGTGAGAGGTCTTTTG
GCGCCGCTGGATCGCAAGAATGGCTGGCAGTTGGCCGAAGCCGCCGGGGATTGCTCGCCGGCGGCGATGCAGGATTTCCTGGCCCGGACCCGTTGGGATG
CCGACGCCGTCCGCGACGACCTCCAGGCCTATGTCATCGAACGGTTGAGCGACGATGAGGCCGTGCTGGTCCTCGATGAAACCGGCTTCGTCAAGAAGGG
CACCCGCTCGGTCGGGGTGAAGCGGCAGTATTCCGGCACCGCCGGACGTATCGAAAACTGCCAGATCGGCGTCTTCCTCGGCTACGCCAGCCGACGCGGC
CGGGTGCTGGTCGACCGCGCTCTCTATCTGCCCGAGGACTGGGCTGGCAACGACGAACGCCGACGGATGGCCGGCGTCCCCAACGCCGTCGGCTTCGCCA
CCAAGCCGAAACTGGGGCGGGCGATGCTGGAGCGCGCCGTGGCAGCCGGCCTGCCCTGCCGCTGGGTGACCGCCGACTCCGTCTACGGCGGGGACTACGC
GCTGCGGCTATGGCTGGAGCGCCAACCGCTGGGCTACGTGCTGGCGATCACCAGCAAACAGCGCGCGCCCATGGGCTTCGATACGGTCAAGGAGCGGACG
ACCGGCTTCACCGCCACCGACTGGCAGCGGTTGAGTGCTGGCGACGGAGCCAAGGGGCCACGCCTCTACGACTGGGCGCACAAAACCTACGCTTCCCTGC
GCGAGGGCTGGTGCCGCGGTCTGCTGGTGCGCCGCTCCATCGCCGAGCCGGACAAACTCACCTACTATCTGACCTTCGCCCCGGAAGGCACGCCGGTCGC
CACATTGGTCCGGGTCGCCGGGTCGCGCTGGACCATCGAGTCCTGCTTCGAGGCGGCGAAGGGCGAGGTCGGGCTCGACCAGTACGAGGTGCGCTCGTGG
ACCGGCTGGCACCGCCACGTCACCTTGGCCATGCTGGCCCTGGCCTTTCTCACCGTCGTGCGCACGGCGGCCATCGGGGGGAGAGGATCAACTCGACCTG
TCCGCCGATCTTCTGCCCTTGACCGTGCCCGAGATCCGCGCCCTCCTGGTCGCCTTGATCGGCCGGCCCCCCGCGCCACCCACCGCCATTATCGATTGGT
CCATCTGGCGAAGACGCCACCAACAGCGAGCACGGCGCTCCCATTGGAAACGACGCACCCAAACCGATAAGGACCGGCTGTAGTA
Protein section
ORF number : 1

 

ORF 1
LengthBeginEndStrandFusion ORF
1293 bp430 aa781370+No
ORF function : Transposase
Chemistry : DDE

ORF sequence :

MTEMTEAAEWSDALEGLVGGLRSFYSAAGRQRALRYVRGLLAPLDRKNGWQLAEAAGDCSPAAMQDFLARTRWDADAVRDDLQAYVIERLSDDEAVLVLD
ETGFVKKGTRSVGVKRQYSGTAGRIENCQIGVFLGYASRRGRVLVDRALYLPEDWAGNDERRRMAGVPNAVGFATKPKLGRAMLERAVAAGLPCRWVTAD
SVYGGDYALRLWLERQPLGYVLAITSKQRAPMGFDTVKERTTGFTATDWQRLSAGDGAKGPRLYDWAHKTYASLREGWCRGLLVRRSIAEPDKLTYYLTF
APEGTPVATLVRVAGSRWTIESCFEAAKGEVGLDQYEVRSWTGWHRHVTLAMLALAFLTVVRTAAIGGRGSTRPVRRSSALDRARDPRPPGRLDRPAPRA
THRHYRLVHLAKTPPTASTALPLETTHPNR

 

Blast result :
Comments
ISAzs6 is 68% aa similar to ISMdi28.
References
1] ISfinder annotation (2010)
2] Kaneko,T., Minamisawa,K., Isawa,T., Nakatsukasa,H., Mitsui,H., Kawaharada,Y., Nakamura,Y., Watanabe,A., Kawashima,K., Ono,A., Shimizu,Y., Takahashi,C., Minami,C., Fujishiro,T., Kohara,M., Katoh,M., Nakazaki,N., Nakayama,S., Yamada,M., Tabata,S. and Sato,S. (2010) DNA Res. 17 (1), 37-50.