ISAzs5

  • Family ISAs1
  • Group
MGE type ISRelated element(s) :
Isoform Synonym(s)
Accession numberTranspositionOriginHost
NC_013854 ND Azospirillum sp.
Azospirillum sp. B510 plasmid pAB510b
Azospirillum sp. B510 plasmid pAB510f
Azospirillum sp. B510 plasmid pAB510a
Azospirillum sp. B510
Azospirillum sp. B510 plasmid pAB510d
Azospirillum sp. B510 plasmid pAB510c
Azospirillum sp. B510 plasmid pAB510e
DNA section
IS Length : 1333 bp

Ends


IR Length : 20/23

IRL : CAGGGCTCCGAAGTCGGGTTAACAGCGGTTTACGAATTGGGAAGTTTCTG
IRR : CAGGGCTCCGAATCCGTGTTAACGGATATTAACGTCGAGGATAGCGTTGA

Insertion site


Left flankDirect repeatRight flankDR Length
GGTGCCGACCGAGCCGTTCC0
TAGGGTGGTGGGCGGACCTCTGGGCGGGGG10
CAGTATGGCGGCAGGCCGATCGGGCAGGTC10
AAGCGTCGGATCGCCGCGGGTGCGACTTCC10
CAGCGGACGCATCTGTCCGGTGGGGCAGGA10
GATAGCGGCAGGAGCCGAGTGC2

DNA sequence

CAGGGCTCCGAAGTCGGGTTAACAGCGGTTTACGAATTGGGAAGTTTCTGATTCGGTGGAGGCTCTCGTCTTGGAAGGTCCTCCCATGAGTTCGATCGAA
GCCGGCTTTGGTGAGAAATCGCGGCTGAAGGCGCTGCTGGAACATTTTTCGCGGATCGACGACCCGCGTGCTCCCTGGCGGGTCGCCTATCCGTTACCGG
AGATCCTGCTTCTGGCGGTGTGCGGGACGATCGCGGATTGCGAGGATTACGAGGCGATCGCCGCCTGGGGTGAAGCGCGGCTCGACTTTCTGCGGCGCTT
CCAGCCCTATCACCATGGGGTGCCGAGCGGACGCTGGCTGACCGTGTTCATGAACCGGATCCCTCCCGGCCTGTTCCAGGACTGCTTCCTGTCCTGGGTG
CGCGAGGCGTGGCCCGACCGGCCGGAACTGGTCGCCATCGACGGCAAGACCTCGCGACGCAGCCATGACCGCAGCCTTGGGCAGGCCCCTTTGCACTTGG
TCTCGGCCTTCGCCACCACCAGCCGTCTGGTCCTCGGCCAGGAGGCCGTCGAGGACAAAGCCAGCGAACTGGCGGCCATTCCGGTTCTGCTGGAGCGCCT
GGCGACCGAGGGCGGGCTCAAGGGCGCGATCGTCACCATCGACGCCATCGCCTGCAACGCCACCATCGCCCAGGCCGTTCGCGACGCCGGGGCCGACTAT
CTGCTGGCGGTCAAAGCCAACCAGCCCACCCTGCGCGACGAGATCGAGAGTTTCTTCACCAGCGCACCGCCGGAGACGCTCGATCACGCCGCCGATATCG
ACAAGGGCCATGGCCGGATCGAGCAGCGCGCGGTCACGGTCGCGCGCCAGGTCGATTGGTTGAGCGGCGCGCGGCGTTTTCCCGGCGAAGTCCGCCTGCC
CGCCGTCGCCGCCATCGTTCGGGTGGTCAGCAAGACCGAGCTGAAGGATCGTTGCCGCACCGACACGCGCTATTACATCACCTCCGCCCCGCTCGCCGCC
CAGGCGGCGGCCGAGGCCGTGCGCGGCCATTGGCGCATCGAGAACCAACTGCACTGGCTGCTCGACGTCGTCTTCAACGAGGACCAGTCCCGCTTGCGCA
AAGGCCACGGCGCCCTGAACATGGCCGTCGTCCGCCACTTCGCCATCAACCTCGTCCGCAAGGCCGCCGAACCCGCCCGGCCGCGCTCCGGATTACGGCG
CGCCACCAAAAAGCCGGCCGCCACTCCCAGACCCACCAGCATCAAGCTCCGCAGGAAGCTGGCAGGCTGGGACACCGATTACCTCAACGCTATCCTCGAC
GTTAATATCCGTTAACACGGATTCGGAGCCCTG
Protein section
ORF number : 1

 

ORF 1
LengthBeginEndStrandFusion ORF
1230 bp409 aa861315+No
ORF function : Transposase
Chemistry : Unknow

ORF sequence :

MSSIEAGFGEKSRLKALLEHFSRIDDPRAPWRVAYPLPEILLLAVCGTIADCEDYEAIAAWGEARLDFLRRFQPYHHGVPSGRWLTVFMNRIPPGLFQDC
FLSWVREAWPDRPELVAIDGKTSRRSHDRSLGQAPLHLVSAFATTSRLVLGQEAVEDKASELAAIPVLLERLATEGGLKGAIVTIDAIACNATIAQAVRD
AGADYLLAVKANQPTLRDEIESFFTSAPPETLDHAADIDKGHGRIEQRAVTVARQVDWLSGARRFPGEVRLPAVAAIVRVVSKTELKDRCRTDTRYYITS
APLAAQAAAEAVRGHWRIENQLHWLLDVVFNEDQSRLRKGHGALNMAVVRHFAINLVRKAAEPARPRSGLRRATKKPAATPRPTSIKLRRKLAGWDTDYL
NAILDVNIR

 

Blast result :
Comments
ISAzs5 is 75% aa similar to ISRce1.
References
1] ISfinder annotation (2010)
2] Kaneko,T., Minamisawa,K., Isawa,T., Nakatsukasa,H., Mitsui,H., Kawaharada,Y., Nakamura,Y., Watanabe,A., Kawashima,K., Ono,A., Shimizu,Y., Takahashi,C., Minami,C., Fujishiro,T., Kohara,M., Katoh,M., Nakazaki,N., Nakayama,S., Yamada,M., Tabata,S. and Sato,S. (2010) DNA Res. 17 (1), 37-50.