ISfinder Mirror Site

ISAzs4

Family IS110
Group IS1111

MGE type ISRelated element(s) :
Isoform Synonym(s) ISazl07_b1

Accession number	Transposition	Origin	Host
NC_013856	ND	Azospirillum sp.	Azospirillum sp. B510 plasmid pAB510b Azospirillum sp. B510 plasmid pAB510a

DNA section

IS Length : 1301 bp

Ends

IR Length : 8/15

IRL : CCTCATAGGAGCCGACCATGGGCGTGACGACAATCGGTCTGGATCTGGCG
IRR : ----TGTGGACGGCCCTCTCGGCGCAAGAGGAGAATTCATGTTGACGATT

Insertion site

Left flank	Direct repeat	Right flank	DR Length
GGAGAAACCA		CATGGTTTCT	0

DNA sequence

CCTCATAGGAGCCGACCATGGGCGTGACGACAATCGGTCTGGATCTGGCGAAGAATGTTTTTCCAGGTTCATGGTATCGACGGCGACGGGAAGATTCTGG
TCCGGCGGCAGTTACGACGAGGCGAGGTGCTGAAGTTATTTCAAGGCCTGCCTGCGTGCCTGGTGGGGATGGAGGCGTGTGGCACGGCGCACCACTGGGC
ACGGGAAATTGCGGCACTTGGTCATACGGTGAAGTTGATGCCGCCCGCCTACGTCAAACCGTACGTGAAACGGGGAAAGACCGACGCGGCGGATGCCGAG
GCGATCTGCGAAGCGGTGACCCGGCCGACAATGCGCTTCGTGGCGGTCAAGACCATCGATCAGCAGGCCGCCTTGATGCTTCACAAGACTCGGGACCTCT
TGGTCCGGCAGCGGACGATGCTGATCAACGTCTTGCGCGGTCACCTCGCGGAATTCGGGATCATCGCGGCCAAAGGGCCCAGCGGGACGAAGGCCGCCAT
CGAGGCTTTGCACGAGGCGCAGGATCGTCTGCCGGAACTGGCGCGACGGGCGCTGCACGGCCTCGTCGATCAGCTCCGCCTGCTCGGCGAGGAAATCGAC
CGGCTGGAGGCGCGTATTCTGGAGTGGCATCGCGTCAGCGACGTCAGCCGCCGTTTGGCGACGATACCCGGCATTGGGCCGATCACAGCCAGTGCGATCG
CCGCGGCGGTGCCGGACGGCTCACTGTTCCGGTCCGGCCGGCAGTTCGTCGCATGGCTCGGCCTGACACCGAAGGCGCACAGCAGCGGCGGCAAAGAGTG
CCAGGTCGGCATCAGCAAGCAGGGCGACGGCGACCTCCGCCGCCTGCTGGTCGTGGGCGCCACGGCGGTCATGCGGTTGGCGCGCCGAGACAATGCGGCT
CGATGCTGGGCAGCGAAGCTGCTGGAGCGCAAGCCGGCCAAACTCGCCGCCGTCGCCCTGGCCAACAAGACCGCGCGCATCGCCTGGGCGGTGCTGACGC
GCGGCGGGACCTACGCGGCACCCACCACCGCATAGTCGAGGTTCGGCTCGGCAGCGGACCAAGTCCCTGCCGGGACGGATGGCGTGAGGGTGGTGAGACG
TGATGACAAACCGGTCAGACCGGGGATCGACCAAACCCACGGGGACCAAGCGTCCGGGAGCGCGCTGAGGTGATTTGGGCGTTGATCCGCGGACTTCATC
AAGGCCAGCGACCAGAGGTCGCGACGACAGGCCGGACACATGGATGCACCCGACCAATCGTCAACATGAATTCTCCTCTTGCGCCGAGAGGGCCGTCCAC
A

Protein section

ORF number : 1

ORF 1

Length		Begin	End	Strand	Fusion ORF
981 bp	326 aa	55	1035	+	No

ORF function : Transposase
Chemistry : DEDD

ORF sequence :

MFFQVHGIDGDGKILVRRQLRRGEVLKLFQGLPACLVGMEACGTAHHWAREIAALGHTVKLMPPAYVKPYVKRGKTDAADAEAICEAVTRPTMRFVAVKT
IDQQAALMLHKTRDLLVRQRTMLINVLRGHLAEFGIIAAKGPSGTKAAIEALHEAQDRLPELARRALHGLVDQLRLLGEEIDRLEARILEWHRVSDVSRR
LATIPGIGPITASAIAAAVPDGSLFRSGRQFVAWLGLTPKAHSSGGKECQVGISKQGDGDLRRLLVVGATAVMRLARRDNAARCWAAKLLERKPAKLAAV
ALANKTARIAWAVLTRGGTYAAPTTA

Blast result :

Comments

ISAzs4 is 78% aa similar to ISMno26. The IS is inserted into a large secondary structure of 134 bp.

References

1] ISfinder annotation (2010)
2] Kaneko,T., Minamisawa,K., Isawa,T., Nakatsukasa,H., Mitsui,H., Kawaharada,Y., Nakamura,Y., Watanabe,A., Kawashima,K., Ono,A., Shimizu,Y., Takahashi,C., Minami,C., Fujishiro,T., Kohara,M., Katoh,M., Nakazaki,N., Nakayama,S., Yamada,M., Tabata,S. and Sato,S. (2010) DNA Res. 17 (1), 37-50