ISAzs3
- Family IS1380
- Group
Isoform Synonym(s) ISazl02_01
| Accession number | Transposition | Origin | Host |
|---|---|---|---|
| NC_013854 | ND | Azospirillum sp. | Azospirillum sp. B510 plasmid pAB510b Azospirillum sp. B510 plasmid pAB510f Azospirillum sp. B510 plasmid pAB510a Azospirillum sp. B510 Azospirillum sp. B510 plasmid pAB510d Azospirillum sp. B510 plasmid pAB510c Azospirillum sp. B510 plasmid pAB510e |
DNA section
IS Length : 1613 bp
Ends
IR Length : 15/22
IRL : CCGGGAAAATTCATGAGGGTTGGCGGGTGTAGCGGCATCCGTTGAGCGGC
IRR : CCGCCTTTATTCACCAGGGTGGCGAGCGGTCTGGTCGCCGCGTTGAGATG
Insertion site
| Left flank | Direct repeat | Right flank | DR Length |
|---|---|---|---|
| TCGTTGACACGTTCCGAGCT | CTAG | CAAGCTCACCGCATGCATAC | 4 |
| GCGACTTCGCCGGCTATTCG | CTAA | TCGCCATCGGGCTGGCGCAG | 4 |
| CAATACGACTGCCCTCAGTC | CTAG | TTACCCGAATTGCAACAAGT | 4 |
| GCCGGCGGAGCGATTTCCCT | CTAG | GTAACGCTCCGCCGGGGCCG | 4 |
| TCTTGAGCCGTCCCCCATTC | CTAG | CCACCTGATCCTTAAACCAA | 4 |
| GACATTGTCCATTCCTGTCCTTAG | CTAGAAGGCTATCAACCTCAACAA | 0 | |
| GCCGCCTTAGGCCAGACCGG | CTAG | GGAATTCGCGCTAAACGGCA | 4 |
| GCCCCAGCCCCGTGACGTCC | CTAG | GCATCACCCCCTAAAGGGGG | 4 |
| CCCACTCCCCTTCCCCATTA | CTAG | AGGCATCCCTATGACCTCGA | 4 |
| GTGGTCATTGGGCCGGTCGG | CTAG | AGTGGAGTTGCGACACTTCC | 4 |
| CCCCGACCACCGCCACGGAC | CTAG | AGCCGAACGGCACGGATGCG | 4 |
| GTTGCCGTGACGAAGCCGGG | CTAG | CTCATCCAGAAATCCAATTC | 4 |
| GGCTCGGGCAGCTTGGCGTA | CTAG | CCCTTCTCTAGCATGGCATT | 4 |
| GATAAAGACCGGCTTTAGTA | CTAG | CAGAGATGTCCACCGATTCG | 4 |
| GCTCTTGCGCCCAAGCCGCC | CTAG | GAGCATCGAATGAATTTACC | 4 |
| TGCCAGACGATGTCACTGTC | CTAG | GGTGGGATGCGGCCGGCACG | 4 |
| GCGGGGCAACCCGCCCGCGG | CTAG | GCAGAAGCCGCGCCGGCGCT | 4 |
| ACGCACCGGCAAGGGCGATC | CTAG | AGCAGGCGCTGTAGTCAGGC | 4 |
| TGATATCGGGAAAGCCGATG | TTAG | CCATCGGGTAAATTCGCTTT | 4 |
| ACCCAGGTGCATAAAGGCGGCTAG | CTAAGTCGCTCCTGCACATGGTCA | 0 | |
| AAGCCGGAGAGCATCCAAGC | CTAA | TTCATAACTCGGGCTGTCTC | 4 |
| TGCAATCGACCTTTCTGCCG | CTAA | GGAACCCGATCAGCGACGCC | 4 |
| TAATTTCCCTGGGACTACGC | CTAG | AGAGATTGGCCGAGCAGCTT | 4 |
| CTCTAAAGCCTGAAGGTTTA | TTAG | TATTTCTCCAAGTCCCTTGA | 4 |
| GCGGACACCTACCCTCATTC | CTAG | TAAACGAGGCACCCTCCCTG | 4 |
| CCCCATTTCGTGAGCGTGAA | TTAG | GCGGTTGAGCCCCGGTGGTT | 4 |
| TCTTGAGCCGTCCCCCATTC | CTAG | CCACCTGATCCTTAAACCTG | 4 |
DNA sequence
CCGGGAAAATTCATGAGGGTTGGCGGGTGTAGCGGCATCCGTTGAGCGGCCGTAGGATTTGGGTGCTGACGCCAACTCCTGCCGTTTCCGGAGCGCCCAC
CCGCCATGGTTGATCATACCCTGCCGCTGCCCGGCCTGTCACCCGTTGCTGGAAAGCCGGTGATCGGGCGCTTTGATGGCGGCCGCCTGTCCTCCGATGG
CGGGCTGCTGGTGCTGCGGGAGGTGGCGAAGCGGCTGCGGATTGCCGATCGGCTGGCCGCCTGTATTGAGGACCCGCGCGATCCAACGCGCACCGTGCAT
TCGCTGGCCGACATCATCGGCTTTCGCCTGCTGGCCATCGCGGCGGGCTACGAGGACGGCAACGACGCCGGCAGCCTACGCTCCGACCCGCTGTTCAAGA
TGGCCCTGGAACGCCTGCCGTCCGAGCGTGACCTTTGCTCGCAAGCCACCATCTCGCGCCTGGAGAACCTGCCGGATACCCGCGCACTGCTGCGCATGGG
CCGAGCCATGGTCGATCTCTACTGCGCGTCCTTTCGCCAGGTGCCCAAGCGCATCGTGCTGGATGTAGACGACACCTTCGACACGGTGCATGGCGGTCAG
CAGTTGCGCCTGTTCAACGCCCATTATGACGAGTACGGCTTCCAGCCCATCGTTGTCTTCGACGGCAACGGCCGCTTTGTCACCGCTGTGCTGCGCCCAG
CCAAGCGGCCCAAGGGGACGGAGATCCGGACCTTCCTGCGTCGCCTGCTCCGCGCCATTCGGGCAAACTGGCCCAAGACCGAGATCCTGCTGCGCGCCGA
CGGCCATTACGCCTGCCCGGAGGTGCTGGACTGGTGCGAGGCGGAGGGGCTCGACTACGTGCTCGGTCTGCCGACCAGCAGCACACTGCGCCGTCACGTC
ACCACGCTGGAGGCCAGCACCGCGGCGCGCTTCCAGGCCATGCCCGGAGCCGACAAGGTGCGCCGCTTCAAGGAATTCTATGACGGGGCCAGCACCTGGA
GCCGGGTCCGCCGCATCGTCGCGCGCGTCGAGGCAGGAGACCAGGGCACCGACAGCCGCTTCATCGTCACCAACCTACGCCACGGCACGGGTCGCTGGCT
GTATGCCGGCCTGTATTGCGCGAGGGGACAGGCGGAAAATCATATAAAAGCCTGGAAATCCCACCTTGCCGCAGACCGGACCTCCTGCACCAAGGCGACG
GCCAACCAGTTCCGCCTGTTCCTGCACGCCGGGGCGTACTGGCTGCTGTGGAGCCTGCGCTCGCTGATGCCGAAACGCTCCCGCTGGCGCACGGTGCAAT
TCGACACCTTGCGGCTGCGCTTGGTGAAGACCGCCGCGCGTATCGTGGAGATGAAGACGCAGATCAAGGTGCACCTGCCGACCAGCGCGCCTGATCAGGC
GATCATCCACCTCGCCCTCGGCCGCATGCCCCGGCTCATCTGCTGAGCACCGGGGCAGCGTGCCCCAGCCGTCACCCAATCCCCTCCACCACCAACTCCG
ACACCGTCACGCCGCCGTCAGCGCGGCGGCCGACGACGCGCGCCCATACAGCCCAACGTTGATCATCTCAACGCGGCGACCAGACCGCTCGCCACCCTGG
TGAATAAAGGCGG
CCGCCATGGTTGATCATACCCTGCCGCTGCCCGGCCTGTCACCCGTTGCTGGAAAGCCGGTGATCGGGCGCTTTGATGGCGGCCGCCTGTCCTCCGATGG
CGGGCTGCTGGTGCTGCGGGAGGTGGCGAAGCGGCTGCGGATTGCCGATCGGCTGGCCGCCTGTATTGAGGACCCGCGCGATCCAACGCGCACCGTGCAT
TCGCTGGCCGACATCATCGGCTTTCGCCTGCTGGCCATCGCGGCGGGCTACGAGGACGGCAACGACGCCGGCAGCCTACGCTCCGACCCGCTGTTCAAGA
TGGCCCTGGAACGCCTGCCGTCCGAGCGTGACCTTTGCTCGCAAGCCACCATCTCGCGCCTGGAGAACCTGCCGGATACCCGCGCACTGCTGCGCATGGG
CCGAGCCATGGTCGATCTCTACTGCGCGTCCTTTCGCCAGGTGCCCAAGCGCATCGTGCTGGATGTAGACGACACCTTCGACACGGTGCATGGCGGTCAG
CAGTTGCGCCTGTTCAACGCCCATTATGACGAGTACGGCTTCCAGCCCATCGTTGTCTTCGACGGCAACGGCCGCTTTGTCACCGCTGTGCTGCGCCCAG
CCAAGCGGCCCAAGGGGACGGAGATCCGGACCTTCCTGCGTCGCCTGCTCCGCGCCATTCGGGCAAACTGGCCCAAGACCGAGATCCTGCTGCGCGCCGA
CGGCCATTACGCCTGCCCGGAGGTGCTGGACTGGTGCGAGGCGGAGGGGCTCGACTACGTGCTCGGTCTGCCGACCAGCAGCACACTGCGCCGTCACGTC
ACCACGCTGGAGGCCAGCACCGCGGCGCGCTTCCAGGCCATGCCCGGAGCCGACAAGGTGCGCCGCTTCAAGGAATTCTATGACGGGGCCAGCACCTGGA
GCCGGGTCCGCCGCATCGTCGCGCGCGTCGAGGCAGGAGACCAGGGCACCGACAGCCGCTTCATCGTCACCAACCTACGCCACGGCACGGGTCGCTGGCT
GTATGCCGGCCTGTATTGCGCGAGGGGACAGGCGGAAAATCATATAAAAGCCTGGAAATCCCACCTTGCCGCAGACCGGACCTCCTGCACCAAGGCGACG
GCCAACCAGTTCCGCCTGTTCCTGCACGCCGGGGCGTACTGGCTGCTGTGGAGCCTGCGCTCGCTGATGCCGAAACGCTCCCGCTGGCGCACGGTGCAAT
TCGACACCTTGCGGCTGCGCTTGGTGAAGACCGCCGCGCGTATCGTGGAGATGAAGACGCAGATCAAGGTGCACCTGCCGACCAGCGCGCCTGATCAGGC
GATCATCCACCTCGCCCTCGGCCGCATGCCCCGGCTCATCTGCTGAGCACCGGGGCAGCGTGCCCCAGCCGTCACCCAATCCCCTCCACCACCAACTCCG
ACACCGTCACGCCGCCGTCAGCGCGGCGGCCGACGACGCGCGCCCATACAGCCCAACGTTGATCATCTCAACGCGGCGACCAGACCGCTCGCCACCCTGG
TGAATAAAGGCGG
Protein section
ORF number : 1
ORF 1
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 1341 bp | 446 aa | 106 | 1446 | + | No |
Chemistry : DDE
ORF sequence :
MVDHTLPLPGLSPVAGKPVIGRFDGGRLSSDGGLLVLREVAKRLRIADRLAACIEDPRDPTRTVHSLADIIGFRLLAIAAGYEDGNDAGSLRSDPLFKMA
LERLPSERDLCSQATISRLENLPDTRALLRMGRAMVDLYCASFRQVPKRIVLDVDDTFDTVHGGQQLRLFNAHYDEYGFQPIVVFDGNGRFVTAVLRPAK
RPKGTEIRTFLRRLLRAIRANWPKTEILLRADGHYACPEVLDWCEAEGLDYVLGLPTSSTLRRHVTTLEASTAARFQAMPGADKVRRFKEFYDGASTWSR
VRRIVARVEAGDQGTDSRFIVTNLRHGTGRWLYAGLYCARGQAENHIKAWKSHLAADRTSCTKATANQFRLFLHAGAYWLLWSLRSLMPKRSRWRTVQFD
TLRLRLVKTAARIVEMKTQIKVHLPTSAPDQAIIHLALGRMPRLIC
LERLPSERDLCSQATISRLENLPDTRALLRMGRAMVDLYCASFRQVPKRIVLDVDDTFDTVHGGQQLRLFNAHYDEYGFQPIVVFDGNGRFVTAVLRPAK
RPKGTEIRTFLRRLLRAIRANWPKTEILLRADGHYACPEVLDWCEAEGLDYVLGLPTSSTLRRHVTTLEASTAARFQAMPGADKVRRFKEFYDGASTWSR
VRRIVARVEAGDQGTDSRFIVTNLRHGTGRWLYAGLYCARGQAENHIKAWKSHLAADRTSCTKATANQFRLFLHAGAYWLLWSLRSLMPKRSRWRTVQFD
TLRLRLVKTAARIVEMKTQIKVHLPTSAPDQAIIHLALGRMPRLIC
Blast result :
Comments
ISAzs3 is 80% aa similar to ISAcr4.
References
1] ISfinder annotation (2010)
2] Kaneko,T., Minamisawa,K., Isawa,T., Nakatsukasa,H., Mitsui,H., Kawaharada,Y., Nakamura,Y., Watanabe,A., Kawashima,K., Ono,A., Shimizu,Y., Takahashi,C., Minami,C., Fujishiro,T., Kohara,M., Katoh,M., Nakazaki,N., Nakayama,S., Yamada,M., Tabata,S. and Sato,S. (2010) DNA Res. 17 (1), 37-50.
2] Kaneko,T., Minamisawa,K., Isawa,T., Nakatsukasa,H., Mitsui,H., Kawaharada,Y., Nakamura,Y., Watanabe,A., Kawashima,K., Ono,A., Shimizu,Y., Takahashi,C., Minami,C., Fujishiro,T., Kohara,M., Katoh,M., Nakazaki,N., Nakayama,S., Yamada,M., Tabata,S. and Sato,S. (2010) DNA Res. 17 (1), 37-50.