ISAzs1

  • Family IS701
  • Group
MGE type ISRelated element(s) :
Isoform Synonym(s) ISazl18_b1
Accession numberTranspositionOriginHost
NC_013856 ND Azospirillum sp.
Azospirillum sp. B510 plasmid pAB510b
DNA section
IS Length : 1508 bp

Ends


IR Length : 19/23

IRL : TAATTCTACTGTGTCATAAAGCTGGAATGGGACAGCGGTTCGTTGTAGTA
IRR : TAGTTCTACTATGTCGTAAAGTTCGTAATTGATGGCAGCATGGACATCGC

Insertion site


Left flankDirect repeatRight flankDR Length
TATCACGACTGGGCCTGTTG0

DNA sequence

TAATTCTACTGTGTCATAAAGCTGGAATGGGACAGCGGTTCGTTGTAGTAAGAGGATTCATGCTTCGTTCAGAGAGGCGGTATGGATCCCCTTGGCACCG
CAGGCGGCATCGAATCACGGTTCGCGGCGTATGTCGAGGGCCTGTCTCCGGCTTTGGGTCACGCTGATCGCGCGGCGCCGTTTCGTGCCTATTGCACCGG
ATTGATCCTTCCAGGCGACCGCAAGAGTGTCGAGCCGATGGCCGCGCGGGTGGAGCCGGGACGTGTCGGCGCCGCCCATCAGTCCTTGCACCATTTTGTT
GCCAAGGCGGCCTGGGAGGATGCGGCTGTGCTGGCCGGGGTGCGCGATCTGGTTTTGCCGGCGCTGTTGGAACGCGGCCCGATCCGCGCCTGGATCATCG
ACGACACCGGCATGCCGAAGAAGGGGCGCCATTCCGTTGGCGTGACGCGCCAGTACTGCGGCCAAATCGGCAAGCAGGACAATTGCCAAGTCGCCGTTTC
GCTGTCGCTGGCAACCGACCACGCCAGCCTTCCCGTCGCCTTCCGGCTTTACCTGCCGGAGACATGGGCCGGCGACGTGGAGCGCCGGGCGCAGACCGGC
GTCCCGGACGGGGTCCTCTTCCAGACCAAGCCGGCCATCGCGCTGGACCAGATCCGCACGGCCATGGCGGCGGGTCTGCCGCCGGGCGTGGTCCTGATGG
ACGCGGGCTACGGCAACGACACCGCCCTGCGCGAAGGCGTGACCGTGCTTGGGCTGACTTACATCGCCGGAGTCCAGTCCTCGCTGACCGTCTGGCCGCC
CGGTGTGGAACCACTGCCGCCCAAACCGTGGAGCGGCAAGGGGCGGCCGCCGAAGCTCCTGCGCCGCGCCCCTGGACATGAACCGATCTCGGTAAAGGCT
CTGGCCGAAGGGTTGGCGCCGGAGGCTTGGCGCTCCGTTACCTGGCGGGAAGGCACGAACGCGCCGCTGGCCTCCCGTTTCGCCGCCGTTCGCGTTCACG
CCGCGCACCGCGACGAGGAGCGGGCGGTGCGTCGCCCGAAGGAGTGGCTGCTGATCGAATGGCCCGAGGATGAAGAGAAGCCGACCAAGTACTGGTTGTC
GACGCTGCCGGAGACAATATCGCTCGACGCCTTGGTGGAGACCGCCAAGTTGCGGTGGCGCATCGAACGCGATTACCTCGAACTCAAGCAGGAGCTCGGC
CTTGGCCATTATGAGGGCCGGGGCTGGCGCGGCTTCCACCATCACGCGACGCTGTGCATCGCCGCCTACGGATTCCTGATCAGGGAACGGGCGGCGATTC
CCCCCTCAGGACCACCTCCCTCCGGGCGCCTCCAAGCTCCTGACCTTCCCGAAAGCTTCCGACCCCGCGGGGCCGCCGCCCCGCCCTGAGCGACACATCC
CGACGTCCATCGCCACCCTGCGCATCGCAATCGCCAGAATGCTCGCCAGGACCCTTCAGCGATGTCCATGCTGCCATCAATTACGAACTTTACGACATAG
TAGAACTA
Protein section
ORF number : 1

 

ORF 1
LengthBeginEndStrandFusion ORF
1308 bp435 aa821389+No
ORF function : Transposase
Chemistry : DDE

ORF sequence :

MDPLGTAGGIESRFAAYVEGLSPALGHADRAAPFRAYCTGLILPGDRKSVEPMAARVEPGRVGAAHQSLHHFVAKAAWEDAAVLAGVRDLVLPALLERGP
IRAWIIDDTGMPKKGRHSVGVTRQYCGQIGKQDNCQVAVSLSLATDHASLPVAFRLYLPETWAGDVERRAQTGVPDGVLFQTKPAIALDQIRTAMAAGLP
PGVVLMDAGYGNDTALREGVTVLGLTYIAGVQSSLTVWPPGVEPLPPKPWSGKGRPPKLLRRAPGHEPISVKALAEGLAPEAWRSVTWREGTNAPLASRF
AAVRVHAAHRDEERAVRRPKEWLLIEWPEDEEKPTKYWLSTLPETISLDALVETAKLRWRIERDYLELKQELGLGHYEGRGWRGFHHHATLCIAAYGFLI
RERAAIPPSGPPPSGRLQAPDLPESFRPRGAAAPP

 

Blast result :
Comments
ISAzs1 is 79% aa similar to ISMmg4.
References
1] ISfinder annotation (2010)
2] Kaneko,T., Minamisawa,K., Isawa,T., Nakatsukasa,H., Mitsui,H., Kawaharada,Y., Nakamura,Y., Watanabe,A., Kawashima,K., Ono,A., Shimizu,Y., Takahashi,C., Minami,C., Fujishiro,T., Kohara,M., Katoh,M., Nakazaki,N., Nakayama,S., Yamada,M., Tabata,S. and Sato,S. (2010) DNA Res. 17 (1), 37-50