ISfinder Mirror Site

ISPrst3

Family IS5
Group IS427

MGE type ISRelated element(s) :
Isoform Synonym(s)

Accession number	Transposition	Origin	Host
NZ_ABJD02000085	ND	Providencia stuartii	Providencia stuartii ATCC 25827

DNA section

IS Length : 869 bp

Ends

IR Length : 17/21

IRL : GAATCTATCCCAAAAGGAATTATCAGATAATACATAATGATAATTTATGA
IRR : GAACCTATCCCAATAGGGTTTTGTTCCAGACAATGATGGCACAGGAAAGA

Insertion site

Left flank	Direct repeat	Right flank	DR Length
ATTTATCATATAA		TTAATGGTAAAAA	0

DNA sequence

GAATCTATCCCAAAAGGAATTATCAGATAATACATAATGATAATTTATGAGGGCAACGTATGGCAGGTAATAAGTGGCAAATCAGTGATGAACTCTGGGA
GAAAATGGCTCCTCTCATCCCGGAGCATAGAACTCAACACCCGCTGGGTACGCACCGCAAGCGGGTTGATAATCGCGCTGCAATGAACGCCATTTTCTTC
GTACTCAGGACGGGTTGCCAGTGGAATGCGCTAAATGCCACCGGTATATGCTCGTCAAGCTCTGCTCATCGCCGATTTCAGGAGTGGCGGGATGCTGGAG
TATTTGAACGCTTCTGGCAAAACGGGTTGCTTGCTTGCGAACAGTTGGACGCTATTGACTGGTCGTGGCTGTCGATGGATGGTTGTATGACCAAATCCCC
CCTGGCAGGCTCAAAAAAACAGGCAAGAACCCAACAGACCGAGGGAAACGGGGCGTAAAGCGCAGTCTGATGACTGACGGGAACGGGCTTCCGCTTGCAC
TGGTTGTCGCTGGAGCAAATACGCACGACATAAAGCTGGTTGCGGATACGCTCGATGCCCTCCAGACGGGCAGACCGGGCAAGAGGCTCCGGTTGTGCAT
GGACAAAGGGTATGAAGCGGAATGGTTGGGATCGTATCTGAGAAGTCGTCGCTATGAGCCTCATGTCCAGTCACGGAAGGATGAATCAGAAGCCATCAAA
AACACGGATTTTAAGGCCCACCGCTGGGTTGTAGAGAGAACACACAGTTGGATGAATCGCTACCGTCGTGTCCTGACTCGTTGGGAGAAAGAGGTCGAAA
ATTACGAGGCAATGCTGCATCTTTCCTGTGCCATCATTGTCTGGAACAAAACCCTATTGGGATAGGTTC

Recoding section

Recoding by frameshift
Frame
Type
Experimentally demonstrated

Stimulators :

Shine-Dalgarno sequence :
Secondary structure :

Recoding motif :

Protein section

ORF number : 3

ORF 1

Length		Begin	End	Strand	Fusion ORF
399 bp	132 aa	60	458	+	No

ORF function : Transposase
Description : First part of the transposase

ORF sequence :

MAGNKWQISDELWEKMAPLIPEHRTQHPLGTHRKRVDNRAAMNAIFFVLRTGCQWNALNATGICSSSSAHRRFQEWRDAGVFERFWQNGLLACEQLDAID
WSWLSMDGCMTKSPLAGSKKQARTQQTEGNGA

Blast result :

ORF 2

Length		Begin	End	Strand	Fusion ORF
507 bp	168 aa	359	865	+	No

ORF function : Transposase
Description : Second part of the transposase

ORF sequence :

LVVAVDGWLYDQIPPGRLKKTGKNPTDRGKRGVKRSLMTDGNGLPLALVVAGANTHDIKLVADTLDALQTGRPGKRLRLCMDKGYEAEWLGSYLRSRRYE
PHVQSRKDESEAIKNTDFKAHRWVVERTHSWMNRYRRVLTRWEKEVENYEAMLHLSCAIIVWNKTLLG

Blast result :

ORF 3

Length		Begin	End	Strand	Fusion ORF
806 bp	268 aa	60	865	+	Yes

ORF function : Transposase
Chemistry : DDE

ORF sequence :

MAGNKWQISDELWEKMAPLIPEHRTQHPLGTHRKRVDNRAAMNAIFFVLRTGCQWNALNATGICSSSSAHRRFQEWRDAGVFERFWQNGLLACEQLDAID
WSWLSMDGCMTKSPLAGSKKTGKNPTDRGKRGVKRSLMTDGNGLPLALVVAGANTHDIKLVADTLDALQTGRPGKRLRLCMDKGYEAEWLGSYLRSRRYE
PHVQSRKDESEAIKNTDFKAHRWVVERTHSWMNRYRRVLTRWEKEVENYEAMLHLSCAIIVWNKTLLG

Blast result :

Comments

ISPrst3 is 95% aa similar to ISPrst2. The third ORF is the putative transposase reconstructed in silico by possible -1 frameshift.

References

1] ISfinder annotation (2010)
2] Sudarsanam,P., Ley,R., Guruge,J., Turnbaugh,P.J., Mahowald,M., Liep,D. and Gordon,J. (2008) Direct submission GenBank.