ISfinder Mirror Site

ISNmo4

Family IS5
Group IS5

MGE type ISRelated element(s) :
Isoform Synonym(s)

Accession number	Transposition	Origin	Host
NZ_AAOF01000003	ND	Nitrococcus mobilis	Nitrococcus mobilis Nb-231

DNA section

IS Length : 1176 bp

Ends

IR Length : 15/18

IRL : GGAGACTCTGAACTATTCCCGGCCACGCCAGTATAATTGCGGCATTGCCA
IRR : GGAACGTCTGAACTATTCGATCTGAAGTCAAACGCATTCGTCTCTGACGA

Insertion site

Left flank	Direct repeat	Right flank	DR Length
ACCTGCGGGG	TTAA	GTATTACCGA	4
GTGCACACAC	CTTA	AGAGTCCAGT	4
AGGCTGACAT	TTAA	TCAGCCCTCC	4

DNA sequence

GGAGACTCTGAACTATTCCCGGCCACGCCAGTATAATTGCGGCATTGCCACCGACCCAGGCGGAACCGATGAAACAAACCACCTTTGCTTCGATGAGTTT
TGCGGCCAAGAAGCGGCGCACGCGCCGGGAGAAGTTTCTCGCCGAGATGGAGCAGGTGGTGCCTTGGGCAAAACTGGTGGCGCTCATTGAGCTGCACTAC
CCCAAGGCGGGCCGGCGTGGCCGCCCGCCGATGCCGCTTGAGTCGATGCTGCGTATTTATTTCATGCAGCAGTGGTACGCGCTCTCCGACCCGGCGATGG
AGGATGCGCTCTATGAGATCGAGTCGATGCGCCGCTTCGCCGGGCTTGAGCTCATCGACGACGCGCTGCCCGACGAGACCACGATTTTGAAGTTTCGCCG
CCTGCTGGAGCGCCACGGCCTGACTGCCCAGATGATGAACGTAATCCACGACGTGCTCGCCGAGCGCGGGGCGCTGCTCAAGGGCGGCACTATGGTCGAT
GCCACGAGTGTGCACGCTCCGGCCTCGACGAAGAACAAGGCGCGCCGGCGCGACCCGCAGATGCACCAGACGCGGAAGGGCAATCAGTGGTATTTCGGCA
TGAAGATCCACGTGGGGGCCGATGTCAACAGCGGCCTTGCTCACACGGTTTCGGTGACTGCAGCCAACGCCTCGGACATCAGCCAATTGCCGCGCCTGCT
GCGCGAGGACGACCGCGCGGTCTTTGGTGATAAAGGCTACGTCGATAACACCCTTAAGCGCGCGGCGCGCGGGGCGGGTGTCCACTGGGGCGTGTCCTTG
AAGGCAAGCTCCAAGCACAAACTCACCGGTGCGAACAAGCGCACCAACCGCAAGCGCTCTTCGGTCCGTGCCCGGGTGGAGCACATTTTCCGGGTGATCA
AGCGCCAATTCGGTTACACCAAGGTGCGCTACAAGGGGCTTGCCAAGAATGCCGCACAGGTGTTCACGCTGATCGGCCTGACGAATCTGTATCTGGTGAG
GAGGCAACTGATGACGAGCTGACAGGCAAACTCCGCCTGCTTGCTCAGAAAAGGGGTTCAGCGGCGCCAAACGGCCCCGTAAATCAGCACTCGGCGGCGG
GAAACCGAGCATTTTTACCCTTCCTCTCGTCAGAGACGAATGCGTTTGACTTCAGATCGAATAGTTCAGACGTTCC

Protein section

ORF number : 1

ORF 1

Length		Begin	End	Strand	Fusion ORF
954 bp	317 aa	69	1022	+	No

ORF function : Transposase
Chemistry : DDE

ORF sequence :

MKQTTFASMSFAAKKRRTRREKFLAEMEQVVPWAKLVALIELHYPKAGRRGRPPMPLESMLRIYFMQQWYALSDPAMEDALYEIESMRRFAGLELIDDAL
PDETTILKFRRLLERHGLTAQMMNVIHDVLAERGALLKGGTMVDATSVHAPASTKNKARRRDPQMHQTRKGNQWYFGMKIHVGADVNSGLAHTVSVTAAN
ASDISQLPRLLREDDRAVFGDKGYVDNTLKRAARGAGVHWGVSLKASSKHKLTGANKRTNRKRSSVRARVEHIFRVIKRQFGYTKVRYKGLAKNAAQVFT
LIGLTNLYLVRRQLMTS

Blast result :

Comments

ISNmo4 is 90% aa similar to ISAzo27.

References

1] ISfinder annotation (2010)
2] Waterbury,J., Ferriera,S., Johnson,J., Kravitz,S., Halpern,A., Remington,K., Beeson,K., Tran,B., Rogers,Y.-H., Friedman,R. and Venter,J.C. (2006) Direct submission GenBank.