ISfinder Mirror Site

ISMem1

Family IS5
Group

MGE type ISRelated element(s) :
Isoform Synonym(s)

Accession number	Transposition	Origin	Host
NC_014002	ND	Methanohalophilus mahii	Methanohalophilus mahii DSM 5219

DNA section

IS Length : 1004 bp

Ends

IR Length : 16/17

IRL : GGCTCTGTAGAAACCCTTTCCTCTAACTTCCACTAATACATTATTATTTT
IRR : GGCTCTGTAGAAATCCTCATTTGAATAATTATTGAAACAGAGATAGCCCT

Insertion site

Left flank	Direct repeat	Right flank	DR Length
GTACGATTTT	CATTTATA	ACACTCCCAA	8
TAACATGTAT	TATGTTTG	TACTATTTAT	8
AAAAATACTG	AAAATTAA	ATAATTTTAT	8

DNA sequence

GGCTCTGTAGAAACCCTTTCCTCTAACTTCCACTAATACATTATTATTTTTATAAGAAGAGGTCCCCAATGTGATTTTGACCATAAAAAACACAAAGGGG
ATGAATGTGTTTTGTCAAGCGATTACTTAAACTTTATCGATACAGCTATGTCTGTAGCTGGAAGATCTCATCTTCCAATCTACAGTTGTAAGTATTCCAA
ACGAAAATATACTCAGCATCAGTTATTGACTTTGGTATTGGTCAAAGAGTACACAGGTAAGAACTATAGAGATGTTGTTAGACTTGTTGAGTTAATGGAT
AGAGTTCAGTCCAAGATTGGTATAAAACACGTACCTCACTTCACAACATTACATAAATTTACCAATAGAATATCTTCTTTCTATTTCAATAGCTTACTTC
ACCAAACTCTGAAACTATTCTATTCACATGGTGAAAAAATACCTCTGATTGCTATTGATTCAAGTGGATTTACAGGTGGACATTGTAGTTACTATTATTC
TGTGAGAACAGGAAAGAAAAGACGGTCGTATCTGAAAACAAGTATAGCTGTTGACGTTGAGAAGTTCATTGTAACTGGTTTTAAGATTTCAGGTAAGCCT
GTGCATGATGCAAAGCATGCATTGACATTGCTTAGACAATGTCACAAAAGGAGAAAAGCTGATTGTTATCTGATGGATAAAGGATATGATTCTGAGAAGA
TACATGCTCTAATCAATGAAGAATTGAAAGCAGAAGCCATCATACCTGTAAGATGTAGAAAAAGGAAGAAGATCAAAGGAAAATATCGTCGAAAAATGAG
AGATGAGTTTGATGAAGACATCTATCATTATAGAAACCTGGTAGAGACGATGTTTTCTGTTTTGAAAAGGAAGTATAGGGAAGAACTGAGGGCAACAAAA
TACAGAAATCAGGTCAAAGAGGTCAAGTTCAAATTATTAATTCATAATATAGATAGGGCTATCTCTGTTTCAATAATTATTCAAATGAGGATTTCTACAG
AGCC

Protein section

ORF number : 1

ORF 1

Length		Begin	End	Strand	Fusion ORF
897 bp	298 aa	108	1004	+	No

ORF function : Transposase
Chemistry : DDE

ORF sequence :

VLSSDYLNFIDTAMSVAGRSHLPIYSCKYSKRKYTQHQLLTLVLVKEYTGKNYRDVVRLVELMDRVQSKIGIKHVPHFTTLHKFTNRISSFYFNSLLHQT
LKLFYSHGEKIPLIAIDSSGFTGGHCSYYYSVRTGKKRRSYLKTSIAVDVEKFIVTGFKISGKPVHDAKHALTLLRQCHKRRKADCYLMDKGYDSEKIHA
LINEELKAEAIIPVRCRKRKKIKGKYRRKMRDEFDEDIYHYRNLVETMFSVLKRKYREELRATKYRNQVKEVKFKLLIHNIDRAISVSIIIQMRISTE

Blast result :

Comments

ISMem1 is 77% aa similar to ISMbu10. There is nos STOP codon inside the element, the transposase use the first after the end of the IS.

References

1] ISfinder annotation (2010)
2] Lucas,S., Copeland,A., Lapidus,A., Glavina del Rio,T., Dalin,E., Tice,H., Bruce,D., Goodwin,L., Pitluck,S., Kyrpides,N., Mavromatis,K., Ivanova,N., Lykidis,A., Saunders,E., Brettin,T., Detter,J.C., Han,C., Land,M., Hauser,L., Markowitz,V., Cheng,J.-F., Hugenholtz,P., Woyke,T., Wu,D., Spring,S., Schneider,S., Schroeder,M., Klenk,H.-P. and Eisen,J.A. (2010) Direct submission GenBank.