ISAra1

  • Family IS5
  • Group IS903
MGE type ISRelated element(s) :
Isoform Synonym(s)
Accession numberTranspositionOriginHost
NC_011983 ND Agrobacterium radiobacter
Agrobacterium radiobacter K84 chromosome 2
DNA section
IS Length : 1116 bp

Ends


IR Length : 11/13

IRL : GGTGCAGGGATCAAATTTCAACATTTTGCATCTATGCGGCCGGGATCGAA
IRR : GGTGCAGCGTTCATCGACAGGTTATCATTCGGTCTTTGATGGGCGGCTTA

Insertion site


Left flankDirect repeatRight flankDR Length
AGATGGAATTAACGCCGACT0

DNA sequence

GGTGCAGGGATCAAATTTCAACATTTTGCATCTATGCGGCCGGGATCGAAAATAGCTCGATTGATCCCGGATGCCGCACAAACATAACGCCGCCCGCCGC
CATCACGTCGGTAAAATGAAGTTCAAAGTGACGAACTGGTCGGAGTATGAGGCGGGCCTTCGCCGCCGTGGCAGTTTAACCCTTTGGATAACACCGGAGG
CGCTGGCAGGCTGGGTCGCTCCGCGGCGCAAGACGCGCGGTGGCCAGCCTCTCTATTCGGATCTGGCGATCGAAACCACACTGATGCTGGGCATCGTCTT
TGGGCTGCGTTTGCGCCAGAGCGAAGGGCTTTTGAGTTCGGTGCTTGCCATGATGGGATTGGACTTGCCTGTGCCCGATCACACCACACTGAGCCGACGG
GCCAGAACCTGGAAATCGTCGGCCGGAGGCGGCGACCGGCAGCACGTGGCGGAAGGCCCCGTTCATATTCTGGTCGATAGTACTGGGCTCAAGGTCTACG
GCGCGGGCCAATGGCTGGAGGAGAAGCATGGCGTAAAGTCCCGGCGTAGCTGGCGAAAGCTGCACCTGGCTGTTGATGCCGACAGTGGCGAGATTATTGC
CCATAGCCCAACAGATCAGGAAGCCGGCGATGCCACGCAGCTGGGGTCGTTGCTGGATCAGATCGACGACGAGATTGATCAATTCACTGCCGATGGAGCC
TATGACGGCGATCCATCCTACGACGCTGTCCTGCGTCACAGCCCAGGCGCAAGGGTCGTCATTCCACCACGCTCGAACGCGGTTGAAAGGCCCAACGCCC
AAGCGTCCTGCCAGAGAGACGATCACACAGCATCCATGCAGATCGATGGTCGGTTGAAATGGCAGACGCCCATCGGCTATAGCAAACGGGCTTTGGCTGA
AACCGCGATGGGTCGATACAAGGGCGTCATCGGTCCGCAGCTACGTGCTCGTTCATTCCGTGCGCAGCAGACTGAAGCTGCGATAAGCGTCGCCATCCTT
AACCGAATGCTTGCTTGCGGACGCCCTAAATCCGTTCGTTGCCGAGCCTTGACGGGAAAAGCCAAATAAGCCGCCCATCAAAGACCGAATGATAACCTGT
CGATGAACGCTGCACC
Protein section
ORF number : 1

 

ORF 1
LengthBeginEndStrandFusion ORF
999 bp332 aa711069+No
ORF function : Transposase
Chemistry : DDE

ORF sequence :

MPHKHNAARRHHVGKMKFKVTNWSEYEAGLRRRGSLTLWITPEALAGWVAPRRKTRGGQPLYSDLAIETTLMLGIVFGLRLRQSEGLLSSVLAMMGLDLP
VPDHTTLSRRARTWKSSAGGGDRQHVAEGPVHILVDSTGLKVYGAGQWLEEKHGVKSRRSWRKLHLAVDADSGEIIAHSPTDQEAGDATQLGSLLDQIDD
EIDQFTADGAYDGDPSYDAVLRHSPGARVVIPPRSNAVERPNAQASCQRDDHTASMQIDGRLKWQTPIGYSKRALAETAMGRYKGVIGPQLRARSFRAQQ
TEAAISVAILNRMLACGRPKSVRCRALTGKAK

 

Blast result :
Comments
ISAra1 is 66% aa similar to ISAzba3.
References
1] ISfinder annotation (2010)
2] Slater,S.C., Goldman,B.S., Goodner,B., Setubal,J.C., Farrand,S.K., Nester,E.W., Burr,T.J., Banta,L., Dickerman,A.W., Paulsen,I., Otten,L., Suen,G., Welch,R., Almeida,N.F., Arnold,F., Burton,O.T., Du,Z., Ewing,A., Godsy,E., Heisel,S., Houmiel,K.L., Jhaveri,J., Lu,J., Miller,N.M., Norton,S., Chen,Q., Phoolcharoen,W., Ohlin,V., Ondrusek,D., Pride,N., Stricklin,S.L., Sun,J., Wheeler,C., Wilson,L., Zhu,H. and Wood,D.W. (2009) J. Bacteriol. 191 (8), 2501-2511.