ISErh1
- Family IS3
- Group IS150
Isoform Synonym(s)
| Accession number | Transposition | Origin | Host |
|---|---|---|---|
| ND | Erysipelothrix rhusiopathiae | Erysipelothrix rhusiopathiae Fujisawa |
DNA section
IS Length : 1280 bp
Ends
IR Length : 35
IRL : GGAACTGACCTAGTTCCGTGGGACTAAAGAAAAAAACCTCTTGTATGAGA
IRR : GGAACTGACCTAGTTCCGTGGGACTAAAGAAAAAACCAAGTTAGGACGAA
Insertion site
| Left flank | Direct repeat | Right flank | DR Length |
|---|---|---|---|
| AACTTAGTAT | ATT | CTCTATACTA | 3 |
| ATTAAAATTT | ACC | TGAAGTGTGT | 3 |
| CTTGTATAAG | ATA | AAATGTGAAA | 3 |
| TGGAACTGAC | CCAG | TTCCATTTTA | 4 |
| AACACATTAG | CGT | TTAGGCTGGA | 3 |
DNA sequence
GGAACTGACCTAGTTCCGTGGGACTAAAGAAAAAAACCTCTTGTATGAGAATTATTGTAAAATAGTTCAATATAGGAGGTTTTTATATGGGAACACGAAC
TATGCATAGCTATGAGACAAAGATGAAAGTTATAGAAATGAAATTGGCAGGCTACTCAAGTAGGTTTATTCAGACTGAACTTGGAATAAAAAATGTAACA
CAAGTCAAAACATGGTGGAGATGGTATCGAAATGGTGAACACTATCGATTTTCTCAACCTGTAGGCAAGCAATATACTTTTGGAAAAGGGCCTGAAGGAG
ACACGGTCGAAGAAACACAAAGACTTAGAATTAAATCTTTGGAGCAACAAATTGAACTATTAAAAAAGTATTTGGAAAGAGAAAGGATGTGGTTCCTGAA
ATAATCATCAAGCTCGTTGAAGAGTATCGCAACACTGTATCTATTAAAGATATCTTGAATCTTTTTGGGGTACCTAAGTCAACGTATTACCGTTGGACTA
AAAAAGAGCAACTAGAGTCTAATAATTATTCTGTCAATGAAGCATTAGTAATTGAACTTTGTAAAGAAAATAAATTTCGTTATGGATATCGAAAAATAAC
TGCATTAATTCGAAAAGAAAGAATTATCAATAAGAACACTGTTCAAAAGATAATGCAGAAACACCAATGTCAATGCCGTGTTAAGGTCAAACGGTATAGA
AAAAACAAAAATCCGAAGATCATTATGCCCAATATCATTAATCGCGACTTTAAATCACTACGTCCTCTAGAGAAATTGGTGACAGATATCACTTACATCC
CTTATGGCCATAAGATGCTCTATTTATCTACGATCATGGATTTATATAATGGTGAGATTATTGCGTCTACATTGAGTGACAGACAAAACCTAGAATGTGT
GGTTGATACATTAAATCAACTTCCGGATATCGTTCAGCCATGTATTCTTCATTCTGATCAAGGGAGTGTCTATACATCAAAAGAGTATCAACTCAAAGTA
AAAAATAAAAGCATTACCATGAGTATGTCCCGTAAGGGTACACCCGCTGATAATGCTCCTATCGAATCGTTTCATGCCTCGCTAAAGTGTGAAACATTCG
AATTAAACCCAGAACTAAAGGGTTCTACTGAAATTGTATCACAAACTGTGATAAACTATTTAAAATATTACAATGAAAATCGAATACAAGAAAAGCTAGG
ATATCAATCTCCCGTAAATTATCGGTTGACTTCGTCCTAACTTGGTTTTTTCTTTAGTCCCACGGAACTAGGTCAGTTCC
TATGCATAGCTATGAGACAAAGATGAAAGTTATAGAAATGAAATTGGCAGGCTACTCAAGTAGGTTTATTCAGACTGAACTTGGAATAAAAAATGTAACA
CAAGTCAAAACATGGTGGAGATGGTATCGAAATGGTGAACACTATCGATTTTCTCAACCTGTAGGCAAGCAATATACTTTTGGAAAAGGGCCTGAAGGAG
ACACGGTCGAAGAAACACAAAGACTTAGAATTAAATCTTTGGAGCAACAAATTGAACTATTAAAAAAGTATTTGGAAAGAGAAAGGATGTGGTTCCTGAA
ATAATCATCAAGCTCGTTGAAGAGTATCGCAACACTGTATCTATTAAAGATATCTTGAATCTTTTTGGGGTACCTAAGTCAACGTATTACCGTTGGACTA
AAAAAGAGCAACTAGAGTCTAATAATTATTCTGTCAATGAAGCATTAGTAATTGAACTTTGTAAAGAAAATAAATTTCGTTATGGATATCGAAAAATAAC
TGCATTAATTCGAAAAGAAAGAATTATCAATAAGAACACTGTTCAAAAGATAATGCAGAAACACCAATGTCAATGCCGTGTTAAGGTCAAACGGTATAGA
AAAAACAAAAATCCGAAGATCATTATGCCCAATATCATTAATCGCGACTTTAAATCACTACGTCCTCTAGAGAAATTGGTGACAGATATCACTTACATCC
CTTATGGCCATAAGATGCTCTATTTATCTACGATCATGGATTTATATAATGGTGAGATTATTGCGTCTACATTGAGTGACAGACAAAACCTAGAATGTGT
GGTTGATACATTAAATCAACTTCCGGATATCGTTCAGCCATGTATTCTTCATTCTGATCAAGGGAGTGTCTATACATCAAAAGAGTATCAACTCAAAGTA
AAAAATAAAAGCATTACCATGAGTATGTCCCGTAAGGGTACACCCGCTGATAATGCTCCTATCGAATCGTTTCATGCCTCGCTAAAGTGTGAAACATTCG
AATTAAACCCAGAACTAAAGGGTTCTACTGAAATTGTATCACAAACTGTGATAAACTATTTAAAATATTACAATGAAAATCGAATACAAGAAAAGCTAGG
ATATCAATCTCCCGTAAATTATCGGTTGACTTCGTCCTAACTTGGTTTTTTCTTTAGTCCCACGGAACTAGGTCAGTTCC
Recoding section
- Recoding by frameshift
- Frame
- Type
- Experimentally demonstrated
Stimulators :
- Shine-Dalgarno sequence :
- Secondary structure :
Recoding motif :
Protein section
ORF number : 3
ORF 1
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 318 bp | 105 aa | 87 | 404 | + | No |
Description : First part of the transposase
ORF sequence :
MGTRTMHSYETKMKVIEMKLAGYSSRFIQTELGIKNVTQVKTWWRWYRNGEHYRFSQPVGKQYTFGKGPEGDTVEETQRLRIKSLEQQIELLKKYLERER
MWFLK
MWFLK
Blast result :ORF 2
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 885 bp | 294 aa | 356 | 1240 | + | No |
Description : Second part of the transposase
ORF sequence :
TIKKVFGKRKDVVPEIIIKLVEEYRNTVSIKDILNLFGVPKSTYYRWTKKEQLESNNYSVNEALVIELCKENKFRYGYRKITALIRKERIINKNTVQKIM
QKHQCQCRVKVKRYRKNKNPKIIMPNIINRDFKSLRPLEKLVTDITYIPYGHKMLYLSTIMDLYNGEIIASTLSDRQNLECVVDTLNQLPDIVQPCILHS
DQGSVYTSKEYQLKVKNKSITMSMSRKGTPADNAPIESFHASLKCETFELNPELKGSTEIVSQTVINYLKYYNENRIQEKLGYQSPVNYRLTSS
QKHQCQCRVKVKRYRKNKNPKIIMPNIINRDFKSLRPLEKLVTDITYIPYGHKMLYLSTIMDLYNGEIIASTLSDRQNLECVVDTLNQLPDIVQPCILHS
DQGSVYTSKEYQLKVKNKSITMSMSRKGTPADNAPIESFHASLKCETFELNPELKGSTEIVSQTVINYLKYYNENRIQEKLGYQSPVNYRLTSS
Blast result :ORF 3
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 1154 bp | 384 aa | 87 | 1240 | + | Yes |
Chemistry : DDE
ORF sequence :
MGTRTMHSYETKMKVIEMKLAGYSSRFIQTELGIKNVTQVKTWWRWYRNGEHYRFSQPVGKQYTFGKGPEGDTVEETQRLRIKSLEQQIELLKKVFGKRK
DVVPEIIIKLVEEYRNTVSIKDILNLFGVPKSTYYRWTKKEQLESNNYSVNEALVIELCKENKFRYGYRKITALIRKERIINKNTVQKIMQKHQCQCRVK
VKRYRKNKNPKIIMPNIINRDFKSLRPLEKLVTDITYIPYGHKMLYLSTIMDLYNGEIIASTLSDRQNLECVVDTLNQLPDIVQPCILHSDQGSVYTSKE
YQLKVKNKSITMSMSRKGTPADNAPIESFHASLKCETFELNPELKGSTEIVSQTVINYLKYYNENRIQEKLGYQSPVNYRLTSS
DVVPEIIIKLVEEYRNTVSIKDILNLFGVPKSTYYRWTKKEQLESNNYSVNEALVIELCKENKFRYGYRKITALIRKERIINKNTVQKIMQKHQCQCRVK
VKRYRKNKNPKIIMPNIINRDFKSLRPLEKLVTDITYIPYGHKMLYLSTIMDLYNGEIIASTLSDRQNLECVVDTLNQLPDIVQPCILHSDQGSVYTSKE
YQLKVKNKSITMSMSRKGTPADNAPIESFHASLKCETFELNPELKGSTEIVSQTVINYLKYYNENRIQEKLGYQSPVNYRLTSS
Blast result :
Comments
There is 5 copies in E. rhusiopathiae strain Fujisawa.
ISErh1 is 70% aa similar to ISSau4.
This IS has atypic IRs: it begins with GG instead of TG.
The third ORF is a putative ORFAB transposase reconstructed in silico by a possible -1 frameshift.
ISErh1 is 70% aa similar to ISSau4.
This IS has atypic IRs: it begins with GG instead of TG.
The third ORF is a putative ORFAB transposase reconstructed in silico by a possible -1 frameshift.
References
1] Yohsuke Ogawa, Yoshihiro Shimoji (2010) Direct submission.