ISfinder Mirror Site

ISPna4

Family IS5
Group IS903

MGE type ISRelated element(s) :
Isoform Synonym(s)

Accession number	Transposition	Origin	Host
CP000530	ND	Polaromonas naphthalenivorans	Polaromonas naphthalenivorans CJ2 plasmid pPNAP01

DNA section

IS Length : 1093 bp

Ends

IR Length : 21

IRL : GGCGTTGTTGCATAAATCCCCCCGGGCGCCCCAATGAGCAGGCTGGCGCG
IRR : GGCGTTGTTGCATAAATCCCCGTGATGGCGCGCCATCCCCCTGGCCCCCA

Insertion site

Left flank	Direct repeat	Right flank	DR Length
CGTCGTGGCT		ACTCGTTGGC	0

DNA sequence

GGCGTTGTTGCATAAATCCCCCCGGGCGCCCCAATGAGCAGGCTGGCGCGCTGGTAGGCTACGTGCTTTTGCCCCGAACATGCCAAAAGACACGCTCCTG
CCCAGCCAGCCCAAAGGCGTTTACCGGGTCAAGAACTGGCCCGAGTACAACGCGGGCCTGATCGAGCGGGGAAATGTGACTGTCTGGATGGACGAAAGGA
TGTTCTCGCCAGCGCTTCAAACGCCAGGCCAGCGCGGGCGCCCGCAGGCGTACTGCGACGCAATGATCCAGATGCTCTTGACGCTCAAGTCGGTGTACCG
CCTGCCGCTGCGGGCGCTTCAAGGCTTCACCACCAGCCTGCAGCACCTGGCGCTGCCGGGTTTGCCGGTGCCCAACTACAGCACGCTGAGCCGAAGGGCC
AAGTCGCTGCGGGTGAGCCTGCCGGTGCTGCGCAACGCGGGCGAGGCGGTGCATCTGCTGGTCGACAGCACCGGCCTGAAGCTCTTTGGCGAGGGCGAGT
GGAAGGTACGCCAACATGGCTACAGCAAACGGCGCAGCTGGCGCAAAGTGCACCTGGCGCTGGACGCGAAAACCGCTCAGGTGTGCGCCGTGTTGATGAC
GCACCGCGACGTCGATGACGCCAGCGTGCTGCCCGAGTTGCTGGCGCAACTGCCGCCACAGACCCAGGTAGAAATTGTCGGCGGCGATGGCGCCTACGAC
ACTAAAGTCGCCCGCGCGGCCATTGCCGGGTGCGCCGCCCTGGCGCTCATTCCTCCCGTCGAGGGCGCCGTTCACTGGCCTGCCAGTCAGGCCGGTGCGC
CAGAGCGCAACGCGGCCATTGACCACATTGCCCAAAGCGGCAAGCAGGACTGGAAGGACAAAAGCGGCTACCACTGCCGCTCCTTGGTGGAGAACCTGAT
GTACCGCTTCAAGACCTTGACGGGCGACAGGCTGTGGGCACGCGATGTGGATGTGCAGGACGCCGAAGTCGCTGTGCGGGTGGGCATCCTCAACCGCATG
ATTGTGCTCGCACGCCCGAACTCCGTCCGGATCGCCTAAAAATTGGGGGCCAGGGGGATGGCGCGCCATCACGGGGATTTATGCAACAACGCC

Protein section

ORF number : 1

ORF 1

Length		Begin	End	Strand	Fusion ORF
960 bp	319 aa	80	1039	+	No

ORF function : Transposase
Chemistry : DDE

ORF sequence :

MPKDTLLPSQPKGVYRVKNWPEYNAGLIERGNVTVWMDERMFSPALQTPGQRGRPQAYCDAMIQMLLTLKSVYRLPLRALQGFTTSLQHLALPGLPVPNY
STLSRRAKSLRVSLPVLRNAGEAVHLLVDSTGLKLFGEGEWKVRQHGYSKRRSWRKVHLALDAKTAQVCAVLMTHRDVDDASVLPELLAQLPPQTQVEIV
GGDGAYDTKVARAAIAGCAALALIPPVEGAVHWPASQAGAPERNAAIDHIAQSGKQDWKDKSGYHCRSLVENLMYRFKTLTGDRLWARDVDVQDAEVAVR
VGILNRMIVLARPNSVRIA

Blast result :

Comments

ISPna4 is 76% aa similar to IS1420.

References

1] ISfinder annotation (2010)
2] Copeland,A., Lucas,S., Lapidus,A., Barry,K., Detter,J.C., Glavina del Rio,T., Hammon,N., Israni,S., Dalin,E., Tice,H., Pitluck,S., Sims,D.R., Brettin,T., Bruce,D., Han,C., Tapia,R., Brainard,J., Schmutz,J., Larimer,F., Land,M., Hauser,L., Kyrpides,N., Kim,E., Madsen,E.L. and Richardson,P. (2006) Direct submission GenBank.