ISAjo1

  • Family IS5
  • Group IS903
MGE type ISRelated element(s) :
Isoform Synonym(s)
Accession numberTranspositionOriginHost
NZ_GG704968 ND Acinetobacter johnsonii
Acinetobacter johnsonii SH046
DNA section
IS Length : 1039 bp

Ends


IR Length : 17/19

IRL : GGCTTTGTTGCACAAAGCTATTCTTAAAGGCTTCTTCAGCAATATAATTT
IRR : GGCTTTGTTGCATAAAGATTTAAAAGTTAAATTTACCCTGTTCTACTCAA

Insertion site


Left flankDirect repeatRight flankDR Length
TATAGTTGCGATGTTGTACTAGG3

DNA sequence

GGCTTTGTTGCACAAAGCTATTCTTAAAGGCTTCTTCAGCAATATAATTTCCAAATGAAGAATCCTGCATCTAAAATTTATCGCACAACGAATTGGTCCT
CTTATAACCAAGCTCTAATCAAGCGAGGAAATATTTCAACTTGGTTCGATCCTCAGACTAAATGGTATGCTCAGCCACAGGCTAAGCATGGAAGAAATCA
AACTTATTCTGATACAGCAATTCAGTGCTGTTTGATGATCAAATCTATCTTTCGACTTTCTTTGCGTATGGTCACAGGCTTTGTGCAAAGCCTAATTCAA
CTCAGTGGGTTAAGTTGGACAGCACCAGATTATTCCACACTTTGCAGACGACAAAAGCAGATTGATATTGCGATCAGCTATCAAAAAAGTCATGAAGGAT
TACATCTATTGATAGACTCAACTGGCCTTAAGTTTTTAGGCGAAGGTGAATGGAAACGTAAGAAACATCAGCCTGAATATCGTCGGCAATGGCGTAAATT
ACATATTGGTATAGATGCTAGAACCTTGCAAATACGAGCTGTACAGCTGACAACAAATAATGTCAGTGATTCGCAAGTCCTTGGAGATTTACTTGATCAA
ATCCCATTGGATGAGCAGATTGATTCAGTCTATACAGACGGGGCTTATGACACCAAACAATGCCGTCAGGTCATTGCAGATCGGCAAGCGTATGCAGTGA
TTCCACCCAGAAAAAATACAAAGCCTTGGAAAGATAACAAAGTTCATTCGCGAGAGCGAAATGAATTACTTCGAACAGTTAAACGTTTCGGCAGGACACT
ATGGAAAAAATGGTCAGGCTATCATCGGCGAAGTTTGGTCGAAACCAAGATGCATTGCATCAAATTATTGGGGGATAAACTCAGTACGAGAAACTTTCAA
AGTCAAGTCAATGAAATGCATGCACGTGTGGCAGTATTAAATAAATTTACGGAGTTAGGTCGACCTCTCACCCAAGTTGTCACTTGAATTTGAGTAGAAC
AGGGTAAATTTAACTTTTAAATCTTTATGCAACAAAGCC
Protein section
ORF number : 1

 

ORF 1
LengthBeginEndStrandFusion ORF
933 bp310 aa55987+No
ORF function : Transposase
Chemistry : DDE

ORF sequence :

MKNPASKIYRTTNWSSYNQALIKRGNISTWFDPQTKWYAQPQAKHGRNQTYSDTAIQCCLMIKSIFRLSLRMVTGFVQSLIQLSGLSWTAPDYSTLCRRQ
KQIDIAISYQKSHEGLHLLIDSTGLKFLGEGEWKRKKHQPEYRRQWRKLHIGIDARTLQIRAVQLTTNNVSDSQVLGDLLDQIPLDEQIDSVYTDGAYDT
KQCRQVIADRQAYAVIPPRKNTKPWKDNKVHSRERNELLRTVKRFGRTLWKKWSGYHRRSLVETKMHCIKLLGDKLSTRNFQSQVNEMHARVAVLNKFTE
LGRPLTQVVT

 

Blast result :
Comments
ISAjo1 is 90% aa similar to ISAha1.
References
1] ISfinder annotation (2010)
2] Ward,D., Feldgarden,M., Earl,A., Young,S.K., Zeng,Q., Koehrsen,M., Alvarado,L., Berlin,A., Bochicchio,J., Borenstein,D., Chapman,S., Chen,Z., Engels,R., Freedman,E., Gellesch,M., Goldberg,J., Griggs,A., Gujja,S., Heilman,E., Heiman,D., Hepburn,T., Howarth,C., Jen,D., Larson,L., Lewis,B., Mehta,T., Park,D., Pearson,M., Roberts,A., Saif,S., Shea,T., Shenoy,N., Sisk,P., Stolte,C., Sykes,S., Thomson,T., Walk,T., White,J., Yandava,C., Seifert,H., Haas,B., Nusbaum,C. and Birren,B. (2009) Direct submission GenBank.