ISfinder Mirror Site

ISAnsp14

Family IS256
Group

MGE type ISRelated element(s) :
Isoform Synonym(s)

Accession number	Transposition	Origin	Host
NC_009675	ND	Anaeromyxobacter sp.	Anaeromyxobacter sp. Fw109-5

DNA section

IS Length : 1629 bp

Ends

IR Length : 11

IRL : GAAGCTCCTCGAAACCAGTAGTGAACAGGGCCTCGCTTGGCGGCGAGGTC
IRR : GAAGCTCCTCGCTCGCTTCGGTGCAGCCCTACTCCCCGCTGTACTTCGCG

Insertion site

Left flank	Direct repeat	Right flank	DR Length
AGAGAGAAGG		ACCGCGCGTA	0

DNA sequence

GAAGCTCCTCGAAACCAGTAGTGAACAGGGCCTCGCTTGGCGGCGAGGTCGAGGAGCCTTCCCATGAACGAGGTTGCCGAGCTTGAGCTGCCCACGCAAG
AGAACGTCCAGGCCGATCTGCGAACCGTCTTCCAGGGGGCCGTCCGGCTCGCGCTGGAGACGGTGCTGGAAGAGGTCGTCCGGGACATGATCGGCGCCCG
CCGCTGGCAGCGTCTCGGCTCGCGAAAGGACTTGCGGAACGGCACGTACCTGCGGCGCCTGCTCACGACCATGGGCGCGATCGACCTGGCGGTGCCGAGG
ACCCGCGAGAACGGCTCGCCGATCGACGTAGTCGGTCGCTACCGCCGGCGCGCCGCCGAGGTCGACGCCGGCATCGTGAGCGCCTACGTCTCCGGCGCGT
CGACCCGCGACGTCGGCAGGATCACCGAGGCGCTGATGGGCGAGCAGGTGAGCCGCTCGACCGTGAGCCGGGTGACGAAGACGCTGGACGAGAAGGTGAA
CGAGCTCCGCAAGGCGCCGATCGCTGGCCCGATCCCGTACCTGTTCCTCGACGCCACCTTCCTCGACGCCCGCTGGGCGCGGACGGTCGAGAACGTCTCT
GCGCTCGTCGCGTACGGCATCGGCCTGGACGGCAAGCGGCAGCTTCTCGCCGTGACCATCGGCGCGGAGGAGTCCGAGGACTCGTGGAGCGACCTCCTGG
CGCAGCTCACCGAGCGGGGCCTCTCGGGCGTCCACCTCGTCGTCGCGGACGCCCACGCCGGCCTCGCGAAGGCGGTCCGCAAGCACATCCCCGAGGCGCC
GCTCCAGCGGTGCACGGTCCACCTCCAGCGGAACGTGCTCGCGAAGGCGCCCCAGCGCCTCCGAGCGCGGCTGGCGAAGGCCGTCACGCACGTATTCGAG
GCACCGAGCAAGGCGGAGGCGAAGAAGCGGGTCGAGGCGCTCGCCGCTGGCCTTGGCAGGCAGGTGCCGGAGGCGATCGCGTGCCTCGAGGCCGGCTTCG
AGGCGGCGACTCACTTCTACGCCTTCCCCCGCGAGCACTGGCACCGCATACGCTCGACGAACGGGCTTGAGCGGCTCCACGGCGAGATCAAGCGCCGCAC
GCGGGCGGTGGGCGCCTTCCCGGACCGGGCCAGCGCCCTCCGGCTCGTCACCGCCGTCGCGCTCCAGGTCACCGCCATCTGGAGCGACCGCCGCTACCTC
GACATGGACCTGCTCCCGGAATCGATGCAGTTTGCGGCGTGACCCAGTACGCGAGGCGCTACATGAGCATTGCCGAAACCGACTCCATCGACATCGTCGC
CGCACGTCCAGACAGCGACGTCGTGCAGTTGGTGATCGCAGATCACCTCGCGTGGGACGACATCGACGCCCACACTCGCCAGCTCCAAGCAAAGATCAAC
ACGTACATCGCCTTCGTCGAGTCGGGCCAGCTGTCCAGGCTGAAGGAGCCAGCCATCCCGCCGTCACCCATCATCCGCATCGTGATCGCGGCGCAGCACA
CGCCACCGACGGCCGCCTCAGACTTCCTGGACCGCGCACGCGCGTTCCTGGCCGGAATCAACATCGGGCTGGAGGTGAACGCGAAGTACAGCGGGGAGTA
GGGCTGCACCGAAGCGAGCGAGGAGCTTC

Protein section

ORF number : 2

ORF 1

Length		Begin	End	Strand	Fusion ORF
1179 bp	392 aa	64	1242	+	No

ORF function : Transposase
Chemistry : DDE

ORF sequence :

MNEVAELELPTQENVQADLRTVFQGAVRLALETVLEEVVRDMIGARRWQRLGSRKDLRNGTYLRRLLTTMGAIDLAVPRTRENGSPIDVVGRYRRRAAEV
DAGIVSAYVSGASTRDVGRITEALMGEQVSRSTVSRVTKTLDEKVNELRKAPIAGPIPYLFLDATFLDARWARTVENVSALVAYGIGLDGKRQLLAVTIG
AEESEDSWSDLLAQLTERGLSGVHLVVADAHAGLAKAVRKHIPEAPLQRCTVHLQRNVLAKAPQRLRARLAKAVTHVFEAPSKAEAKKRVEALAAGLGRQ
VPEAIACLEAGFEAATHFYAFPREHWHRIRSTNGLERLHGEIKRRTRAVGAFPDRASALRLVTAVALQVTAIWSDRRYLDMDLLPESMQFAA

Blast result :

ORF 2

Length		Begin	End	Strand	Fusion ORF
339 bp	112 aa	1263	1601	+	No

ORF function : Passenger Gene
Annotation : Hypothetical proteinDescription :

ORF sequence :

MSIAETDSIDIVAARPDSDVVQLVIADHLAWDDIDAHTRQLQAKINTYIAFVESGQLSRLKEPAIPPSPIIRIVIAAQHTPPTAASDFLDRARAFLAGIN
IGLEVNAKYSGE

Blast result :

Comments

ISAnsp14 orfA (the transposase) is 88% aa similar to ISAde5.
ISAnsp14 orfB is a passenger gene.

References

1] ISfinder annotation (2010)
2] Copeland,A., Lucas,S., Lapidus,A., Barry,K., Glavina del Rio,T., Dalin,E., Tice,H., Pitluck,S., Sims,D., Brettin,T., Bruce,D., Detter,J.C., Han,C., Schmutz,J., Larimer,F., Land,M., Hauser,L., Kyrpides,N., Lykidis,A., Fields,M. and Richardson,P.
(2007) Direct submission GenBank.