ISBco4
- Family IS1182
- Group
Isoform Synonym(s)
| Accession number | Transposition | Origin | Host |
|---|---|---|---|
| NZ_AAWV02000001 | ND | Bacillus coagulans | Bacillus coagulans 36D1 |
DNA section
IS Length : 1560 bp
Ends
IR Length : 15/17
IRL : GAATGAATTTCATATTTCATAGTCCCTGCGCCCCAAGGGTTTCAGAGCAC
IRR : GAATCAATTTCATAATTGCTCTTTTTAAAAAGACAGAGACCTGCAGAATT
Insertion site
| Left flank | Direct repeat | Right flank | DR Length |
|---|---|---|---|
| ATTGCTATAC | ACTA | TTTTAATCAT | 4 |
DNA sequence
GAATGAATTTCATATTTCATAGTCCCTGCGCCCCAAGGGTTTCAGAGCACAAAAAAGGAAGTACCTCCCCAAGTCTTGTATAATGGTAATTGCCAAGAAA
ACCAAAAAGACAGGAGGGACTTCCCTTTGACCATTATACGACAAATAAGTTTTTTTGGCATCCAAGAATTATATGAAATGGAGCCTACCCACCGTTATGA
TGCCATTATTTCAGCTATAAATTTGGATAAAATCTGCCATGCCATCTCTAAGAAATCCAATCTTGGCGCACCTGAAGCATTAAACTATGCAGCCATGGTG
ATCTCTGTTTTCATTCGGATTGTTGAACGAATCCCAACGATCAAAGACCTTGTAAAACGGCTTAATGAAGATATTGCTTTTAAACTAAACTGTGGCTTTC
TTGTATCAGACCATATACCTTCTGAGGCTTCTTATTCCCGGCTCATTACCAAACTAGAAGAGACCAATATTTTGGAAGTATCACTGAAAGAAGTGATTAC
ACAGGCCATTTCCGAAGGATTTATCATGGATGATACAGTCGCCATCGATGCCACGCATTTTGAAGCCCGGGACCAGGCACCACCCAAAAAAGAAAAACCG
GACAAAGAGCCAAAAAAACGTGGCAGAAAGCCCAAAGAAGAACGGGAAAAATGGCTGGCTGAACAAGCTGAAAAAGAAGCAAATCTGCCCATTTATGAAA
AGAAAATTGAAGCCCAACTGGATGCCCCTTTGGATGAACTGCGATCTCAAGTTCCGATAGATCCGAAATGGGGCATTAAAAAGAACAGTGAAGGCAAGAA
TGTGTTTTGGTTTGGCTATAAGGGCCATTTGGCAGTTGGAGCCTCCAGCCAATATATCTTACAGACTTTGTTCTCCTCGGGGAACTTGAATGACGGAAAG
GCGGCAATCCCATTGCTTAAAGGGATTCAGCAACGATTAAACTTGCCGGCTTTGCATTATACAACAATGGATGCCGGTTACGATTATGAACCGATCTATC
AACAGGTTTACCGTATGGGATGCCAATCCGTGATTGCGTACAATAAACGGAATGAACCGGAACTAGAGGGCTTTGACAAACATTTCGCTCTCACCTGTTT
CAGAGAGTATTCTTATCGTTATGACAGCTACGACGCCAAGTATGAAACATTGAAGTACACCCGGCCAAAAGAATGTGCAGACTGCCCACTGGCCAATGAA
GGCATCTGTCAAAAAGTGTATAAAGTAAAGATTACGACAGATCTTCGTAAATATACAGCTCCTGCCCGGGGATCTAAGGCATGGAAGAACATTTTCAAAC
GCCGGACTGCAGTAGAACGAGTAAACGCCTATTTGAAGGAATTCTTTCAGCTTAACAATGTTCGATACAGGACCGGCAAACGGGCAAAAGTACATTTCGA
TCTTGTAACTCTAATTTATAACGCATCAAAGTTAGCCGCAGACCGCATAAATGCCATGATAAAACAAACACAACAAGCTGCATAAGCATTAAAAATTTTT
ACAAGTTGAGAATTCTGCAGGTCTCTGTCTTTTTAAAAAGAGCAATTATGAAATTGATTC
ACCAAAAAGACAGGAGGGACTTCCCTTTGACCATTATACGACAAATAAGTTTTTTTGGCATCCAAGAATTATATGAAATGGAGCCTACCCACCGTTATGA
TGCCATTATTTCAGCTATAAATTTGGATAAAATCTGCCATGCCATCTCTAAGAAATCCAATCTTGGCGCACCTGAAGCATTAAACTATGCAGCCATGGTG
ATCTCTGTTTTCATTCGGATTGTTGAACGAATCCCAACGATCAAAGACCTTGTAAAACGGCTTAATGAAGATATTGCTTTTAAACTAAACTGTGGCTTTC
TTGTATCAGACCATATACCTTCTGAGGCTTCTTATTCCCGGCTCATTACCAAACTAGAAGAGACCAATATTTTGGAAGTATCACTGAAAGAAGTGATTAC
ACAGGCCATTTCCGAAGGATTTATCATGGATGATACAGTCGCCATCGATGCCACGCATTTTGAAGCCCGGGACCAGGCACCACCCAAAAAAGAAAAACCG
GACAAAGAGCCAAAAAAACGTGGCAGAAAGCCCAAAGAAGAACGGGAAAAATGGCTGGCTGAACAAGCTGAAAAAGAAGCAAATCTGCCCATTTATGAAA
AGAAAATTGAAGCCCAACTGGATGCCCCTTTGGATGAACTGCGATCTCAAGTTCCGATAGATCCGAAATGGGGCATTAAAAAGAACAGTGAAGGCAAGAA
TGTGTTTTGGTTTGGCTATAAGGGCCATTTGGCAGTTGGAGCCTCCAGCCAATATATCTTACAGACTTTGTTCTCCTCGGGGAACTTGAATGACGGAAAG
GCGGCAATCCCATTGCTTAAAGGGATTCAGCAACGATTAAACTTGCCGGCTTTGCATTATACAACAATGGATGCCGGTTACGATTATGAACCGATCTATC
AACAGGTTTACCGTATGGGATGCCAATCCGTGATTGCGTACAATAAACGGAATGAACCGGAACTAGAGGGCTTTGACAAACATTTCGCTCTCACCTGTTT
CAGAGAGTATTCTTATCGTTATGACAGCTACGACGCCAAGTATGAAACATTGAAGTACACCCGGCCAAAAGAATGTGCAGACTGCCCACTGGCCAATGAA
GGCATCTGTCAAAAAGTGTATAAAGTAAAGATTACGACAGATCTTCGTAAATATACAGCTCCTGCCCGGGGATCTAAGGCATGGAAGAACATTTTCAAAC
GCCGGACTGCAGTAGAACGAGTAAACGCCTATTTGAAGGAATTCTTTCAGCTTAACAATGTTCGATACAGGACCGGCAAACGGGCAAAAGTACATTTCGA
TCTTGTAACTCTAATTTATAACGCATCAAAGTTAGCCGCAGACCGCATAAATGCCATGATAAAACAAACACAACAAGCTGCATAAGCATTAAAAATTTTT
ACAAGTTGAGAATTCTGCAGGTCTCTGTCTTTTTAAAAAGAGCAATTATGAAATTGATTC
Protein section
ORF number : 1
ORF 1
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 1359 bp | 452 aa | 127 | 1485 | + | No |
Chemistry : DDE
ORF sequence :
MTIIRQISFFGIQELYEMEPTHRYDAIISAINLDKICHAISKKSNLGAPEALNYAAMVISVFIRIVERIPTIKDLVKRLNEDIAFKLNCGFLVSDHIPSE
ASYSRLITKLEETNILEVSLKEVITQAISEGFIMDDTVAIDATHFEARDQAPPKKEKPDKEPKKRGRKPKEEREKWLAEQAEKEANLPIYEKKIEAQLDA
PLDELRSQVPIDPKWGIKKNSEGKNVFWFGYKGHLAVGASSQYILQTLFSSGNLNDGKAAIPLLKGIQQRLNLPALHYTTMDAGYDYEPIYQQVYRMGCQ
SVIAYNKRNEPELEGFDKHFALTCFREYSYRYDSYDAKYETLKYTRPKECADCPLANEGICQKVYKVKITTDLRKYTAPARGSKAWKNIFKRRTAVERVN
AYLKEFFQLNNVRYRTGKRAKVHFDLVTLIYNASKLAADRINAMIKQTQQAA
ASYSRLITKLEETNILEVSLKEVITQAISEGFIMDDTVAIDATHFEARDQAPPKKEKPDKEPKKRGRKPKEEREKWLAEQAEKEANLPIYEKKIEAQLDA
PLDELRSQVPIDPKWGIKKNSEGKNVFWFGYKGHLAVGASSQYILQTLFSSGNLNDGKAAIPLLKGIQQRLNLPALHYTTMDAGYDYEPIYQQVYRMGCQ
SVIAYNKRNEPELEGFDKHFALTCFREYSYRYDSYDAKYETLKYTRPKECADCPLANEGICQKVYKVKITTDLRKYTAPARGSKAWKNIFKRRTAVERVN
AYLKEFFQLNNVRYRTGKRAKVHFDLVTLIYNASKLAADRINAMIKQTQQAA
Blast result :
Comments
ISBco4 is 88% aa similar to IS656.
References
1] ISfinder annotation (2010)
2] Copeland,A., Lucas,S., Lapidus,A., Barry,K., Glavina del Rio,T., Dalin,E., Tice,H., Bruce,D., Goodwin,L., Chertkov,O., Brettin,T., Han,C., Detter,C., Pitluck,S., Patel,M., Ou,M., Rhee,M.S., Harbrucker,R., Ingram,L.O. and Shanmugam,K.T. (2007) Direct submission GenBank.
2] Copeland,A., Lucas,S., Lapidus,A., Barry,K., Glavina del Rio,T., Dalin,E., Tice,H., Bruce,D., Goodwin,L., Chertkov,O., Brettin,T., Han,C., Detter,C., Pitluck,S., Patel,M., Ou,M., Rhee,M.S., Harbrucker,R., Ingram,L.O. and Shanmugam,K.T. (2007) Direct submission GenBank.