ISSen3
- Family IS21
- Group
Isoform Synonym(s) IS100SC
| Accession number | Transposition | Origin | Host |
|---|---|---|---|
| AY509004 | ND | Salmonella enterica | Salmonella enterica subsp. enterica sv. Choleraesuis OU7519 plasmid pOU7519 Salmonella enterica Choleraesuis SC-B67 plasmid pSC138 |
DNA section
IS Length : 1966 bp
Ends
IR Length : 22/28
IRL : TGTCAACAACGGGTAAAAAGTGATCCACTTACCGCCACCACCAACGGTTT
IRR : TGTTACCAACGGTGCAAAACTGATCCACCCCAGCGGTTGAAAATTGATCC
Insertion site
| Left flank | Direct repeat | Right flank | DR Length |
|---|---|---|---|
| GTAAACAGGTTAATG | GTGAT | TTGGTTCTTGCAAACC | 5 |
| GTAAACAGGTTAATG | GTGAT | TGGTTCTTGCAAACCA | 5 |
DNA sequence
TGTCAACAACGGGTAAAAAGTGATCCACTTACCGCCACCACCAACGGTTTAATATTGATCCACCTTGTTTACTCAGGATTAGCTTCAGCTATAACCCCGG
CCTTTCGTTTCTGCTTCAGTCGATAGCTTTCCCCTTTTATTTGCACGACGTGTGAGTGATGTAAGATCCGGTCCAGCATCGCCGATGTCAGCGCTGCATC
ACCGGCGAACGTCTGATCCCACTGCCCGAACGGCAGGTTGGAGGTCAGGATCATCGCGCTCTTCTCGTAACGTTTGGCGATGACCTGGAAGAACAGCTTG
GCTTCCTCCTGACTGAACGGCAGATAACCTATTTCATCGATGATAAGCAGCTTCGGGGCCATGACACCACGATTGAGAGTCGTTTTGTAACGGCCCTGAC
GCTGTGAAGTGGACAGCTGTAGCAGCAGGTCCGCTGCTGTTGTGAAGCGAACCTTGATGCCCGCCCGTACTGCTTCGTAGCCCATGGCTATCGCCAGATG
CGTTTTTCCCACGCCCGATGGCCCCAGCAACACGATGTTTTCGTTACGCTCTATGAAGCTCAGGGATCGCAGCGACTGGATTTGCTTCTGAGGAGCGCCG
GTGGCGAAGGTGAAGTCGTACTCCTCGAACGTCTTTACCGCCGGGAAGGCTGCCATCCGCGTGTACATCGCCTGTTTACGCTGATGCCGGGCCAGTTTCT
CCTCATGTAACAGGTGCTCCAGGAAGTCCATGTAGCTCCATTCCTGATCCACCGCCTGTTGCGACAGCGCCGGCGCTGCGCCGATAAGACTGTCCAGCTG
GAGCTGTTCGGCAAGCACCATCAGCCGTTGATGTTGCAGTTCGACCATCATGCGGCTCCTCTGCAGAACGTGTCATAGATGGAGAGCGGATGATGCAACG
GCTGCCTGTCGAAGGTCATCAGGCTTTCATCAACCTGCACGTCATACTGTTTTTTCTCCGGTGGCAGTGCCAGCATGGATTGCTGTTCCTCCACCCAGCG
ATCGCAGGGGCGGGTCTGGATAGTTTCATGCTTACGTTGATTGGCCACATCGTACAGCCAGCGCAGGCCGTAACGGTTTGCGGTTTCAACATCGACGGTG
ATCCCCATCGGACGCAGGCGTGTCATTAACGGGATATAGAAGCTGTTGCGGGCGTACTGCACCATCCTCTCCACCTTGCCTTTAGTCTGCGCCCTGAAGG
GACGGCACAGGCGGGGAGAGAAGCCCATCTCTTTGCCGAACTGCCACAGGGAAGGATGGAACCGGTGCTGCCCGGTCTGGTAAGCATCACGCTGCAGCAC
CACCGTTTTCATATTGTCGTACAGGACTTCCTGCGGTACACCGCCGAAGAAGCTGAACGCATTGCGGTGACAGGCTTCCAGCGTGTCGTAGCGCATGTTG
TCGGTGAACTCGATGTAAAGCATTCTGCTGTATCCCAGAACAGCGACGAACACATGCAGGGGTGACTTGCCGTTTCGCATGGTCCCCCAGTCAACCTGCA
TCTGCCGTCCGGGCTCGGTTTCGAAGCGAACGACCGGTTCTGCCTGTGCTGGCAGGGTCTGTTTACGGATGAACTCTCTCAGGATAGTAAGCCCTCCACG
ATAGCCCAGCTCCATGATTTCCCTGGCAATAACGGTCGCCGGGATTTTGTAGGGATGCGCATCGCTGATCCGCTTAGAGATGTAATCACGGTATTCATCG
AGCAGTGATGATGGTGCCGGGCGTGGTGAATACTGCGGCTTTTCAGATTTGGCTTTCAGGTGGCTGCGGACAGTATTGCGCGAAATACCCAGCTCCCTGG
CAATGGCCCGGATACTCATTCCCCGCTTGTGCAGGACTTTAATTTCCATACGAATCTCAAAAGTGATCATAAGCTCCCCTGTATTCAGAGGAGCAGATTA
ACCCCTGGATCAATTTTCAACCGCTGGGGTGGATCAGTTTTGCACCGTTGGTAACA
CCTTTCGTTTCTGCTTCAGTCGATAGCTTTCCCCTTTTATTTGCACGACGTGTGAGTGATGTAAGATCCGGTCCAGCATCGCCGATGTCAGCGCTGCATC
ACCGGCGAACGTCTGATCCCACTGCCCGAACGGCAGGTTGGAGGTCAGGATCATCGCGCTCTTCTCGTAACGTTTGGCGATGACCTGGAAGAACAGCTTG
GCTTCCTCCTGACTGAACGGCAGATAACCTATTTCATCGATGATAAGCAGCTTCGGGGCCATGACACCACGATTGAGAGTCGTTTTGTAACGGCCCTGAC
GCTGTGAAGTGGACAGCTGTAGCAGCAGGTCCGCTGCTGTTGTGAAGCGAACCTTGATGCCCGCCCGTACTGCTTCGTAGCCCATGGCTATCGCCAGATG
CGTTTTTCCCACGCCCGATGGCCCCAGCAACACGATGTTTTCGTTACGCTCTATGAAGCTCAGGGATCGCAGCGACTGGATTTGCTTCTGAGGAGCGCCG
GTGGCGAAGGTGAAGTCGTACTCCTCGAACGTCTTTACCGCCGGGAAGGCTGCCATCCGCGTGTACATCGCCTGTTTACGCTGATGCCGGGCCAGTTTCT
CCTCATGTAACAGGTGCTCCAGGAAGTCCATGTAGCTCCATTCCTGATCCACCGCCTGTTGCGACAGCGCCGGCGCTGCGCCGATAAGACTGTCCAGCTG
GAGCTGTTCGGCAAGCACCATCAGCCGTTGATGTTGCAGTTCGACCATCATGCGGCTCCTCTGCAGAACGTGTCATAGATGGAGAGCGGATGATGCAACG
GCTGCCTGTCGAAGGTCATCAGGCTTTCATCAACCTGCACGTCATACTGTTTTTTCTCCGGTGGCAGTGCCAGCATGGATTGCTGTTCCTCCACCCAGCG
ATCGCAGGGGCGGGTCTGGATAGTTTCATGCTTACGTTGATTGGCCACATCGTACAGCCAGCGCAGGCCGTAACGGTTTGCGGTTTCAACATCGACGGTG
ATCCCCATCGGACGCAGGCGTGTCATTAACGGGATATAGAAGCTGTTGCGGGCGTACTGCACCATCCTCTCCACCTTGCCTTTAGTCTGCGCCCTGAAGG
GACGGCACAGGCGGGGAGAGAAGCCCATCTCTTTGCCGAACTGCCACAGGGAAGGATGGAACCGGTGCTGCCCGGTCTGGTAAGCATCACGCTGCAGCAC
CACCGTTTTCATATTGTCGTACAGGACTTCCTGCGGTACACCGCCGAAGAAGCTGAACGCATTGCGGTGACAGGCTTCCAGCGTGTCGTAGCGCATGTTG
TCGGTGAACTCGATGTAAAGCATTCTGCTGTATCCCAGAACAGCGACGAACACATGCAGGGGTGACTTGCCGTTTCGCATGGTCCCCCAGTCAACCTGCA
TCTGCCGTCCGGGCTCGGTTTCGAAGCGAACGACCGGTTCTGCCTGTGCTGGCAGGGTCTGTTTACGGATGAACTCTCTCAGGATAGTAAGCCCTCCACG
ATAGCCCAGCTCCATGATTTCCCTGGCAATAACGGTCGCCGGGATTTTGTAGGGATGCGCATCGCTGATCCGCTTAGAGATGTAATCACGGTATTCATCG
AGCAGTGATGATGGTGCCGGGCGTGGTGAATACTGCGGCTTTTCAGATTTGGCTTTCAGGTGGCTGCGGACAGTATTGCGCGAAATACCCAGCTCCCTGG
CAATGGCCCGGATACTCATTCCCCGCTTGTGCAGGACTTTAATTTCCATACGAATCTCAAAAGTGATCATAAGCTCCCCTGTATTCAGAGGAGCAGATTA
ACCCCTGGATCAATTTTCAACCGCTGGGGTGGATCAGTTTTGCACCGTTGGTAACA
Protein section
ORF number : 2
ORF 1
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 1023 bp | 340 aa | 87 | 1109 | + | No |
Chemistry : DDE
ORF sequence :
MITFEIRMEIKVLHKRGMSIRAIARELGISRNTVRSHLKAKSEKPQYSPRPAPSSLLDEYRDYISKRISDAHPYKIPATVIAREIMELGYRGGLTILREF
IRKQTLPAQAEPVVRFETEPGRQMQVDWGTMRNGKSPLHVFVAVLGYSRMLYIEFTDNMRYDTLEACHRNAFSFFGGVPQEVLYDNMKTVVLQRDAYQTG
QHRFHPSLWQFGKEMGFSPRLCRPFRAQTKGKVERMVQYARNSFYIPLMTRLRPMGITVDVETANRYGLRWLYDVANQRKHETIQTRPCDRWVEEQQSML
ALPPEKKQYDVQVDESLMTFDRQPLHHPLSIYDTFCRGAA
IRKQTLPAQAEPVVRFETEPGRQMQVDWGTMRNGKSPLHVFVAVLGYSRMLYIEFTDNMRYDTLEACHRNAFSFFGGVPQEVLYDNMKTVVLQRDAYQTG
QHRFHPSLWQFGKEMGFSPRLCRPFRAQTKGKVERMVQYARNSFYIPLMTRLRPMGITVDVETANRYGLRWLYDVANQRKHETIQTRPCDRWVEEQQSML
ALPPEKKQYDVQVDESLMTFDRQPLHHPLSIYDTFCRGAA
Blast result :ORF 2
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 780 bp | 259 aa | 1109 | 1888 | + | No |
AG : IS21 helper
ORF sequence :
MVELQHQRLMVLAEQLQLDSLIGAAPALSQQAVDQEWSYMDFLEHLLHEEKLARHQRKQAMYTRMAAFPAVKTFEEYDFTFATGAPQKQIQSLRSLSFIE
RNENIVLLGPSGVGKTHLAIAMGYEAVRAGIKVRFTTAADLLLQLSTSQRQGRYKTTLNRGVMAPKLLIIDEIGYLPFSQEEAKLFFQVIAKRYEKSAMI
LTSNLPFGQWDQTFAGDAALTSAMLDRILHHSHVVQIKGESYRLKQKRKAGVIAEANPE
RNENIVLLGPSGVGKTHLAIAMGYEAVRAGIKVRFTTAADLLLQLSTSQRQGRYKTTLNRGVMAPKLLIIDEIGYLPFSQEEAKLFFQVIAKRYEKSAMI
LTSNLPFGQWDQTFAGDAALTSAMLDRILHHSHVVQIKGESYRLKQKRKAGVIAEANPE
Blast result :
Comments
ISSen3 is 93%(istA) and 98%(istB) aa similar to IS100kyp.
Salmonella enterica serotype Choleraesuis is usually multi-drug resistant and a cause of serious systemic infections. Salmonella enterica Choleraesuis SC-B67 habors two plasmids, pSCV50 and pSC138. ISSen3 was found in pSC138.
Salmonella enterica serotype Choleraesuis is usually multi-drug resistant and a cause of serious systemic infections. Salmonella enterica Choleraesuis SC-B67 habors two plasmids, pSCV50 and pSC138. ISSen3 was found in pSC138.
References
1] Chyi-Liang Chen (2010) Direct submission.