ISRel11
- Family IS5
- Group IS427
Isoform Synonym(s)
| Accession number | Transposition | Origin | Host |
|---|---|---|---|
| NC_004041 | ND | Rhizobium etli | Rhizobium etli CFN 42 symbiotic plasmid p42d Rhizobium etli Kim 5 |
DNA section
IS Length : 862 bp
Ends
IR Length : 23/26
IRL : GGCTGTAGTGACAAATTAATGATTTGGGATTCCGTTTTGGGAGCAAATCA
IRR : GGCTGCAGTGACAATTTAATCATTTGAGCCAGAGCATGATGGCGGCGATT
Insertion site
| Left flank | Direct repeat | Right flank | DR Length |
|---|---|---|---|
| CCTGCTAGAC | TA | GAACGTGGTG | 2 |
| CTCGAGGAAC | TA | GCAGTTTCCT | 2 |
DNA sequence
GGCTGTAGTGACAAATTAATGATTTGGGATTCCGTTTTGGGAGCAAATCAGATTCAACGCTGACTTTTGGAGGTTGGCGATGGATGGTGAGGTTCTTCGA
GACGATCAATGGGAGCGGCTGACGCCGTTAGTGCCCGGCGGTCGCAAAGGCCGGCGCGGTCCGCGTAGTGACGGACGCCGGTTCTTCGATGCGGTGTTGT
GGCTCGCCCGGTCGGGTGCGCGCTGGCGTGATCTGCCGGAAGACCGCTTCGGCCCCTATCAGACGATCAAACGGCGCTACTATCGCTGGATCGAACAGGG
CATCTTCGACAAGATTTTCGAAGCCGTCGCGATCGATCCGGATATGGAGTGGTTGTCCATCGACGCCACGGTGATCCGGGCACAAGCGCAAGCGGCAGGC
GGACGCCGAAAAAGGGGGGACCGCAAGCCCAGGCTCTCGGCCGTTCCCGCGGGGGATTTGGCAGCAAGATCCATGCAGTAGTCGATGCGCTCGGCTTGCC
GGTGCGCCTTCACCTGGGACCCGGCCAGCAGAATGATATGGCGCCTGCCTGCGATCTCGTCCGGGGGCTGCCGGCCAAGCAGGTGCTCGCCGACCGCGCC
TATGACGCCGACAGCCTGCATGACATTATTCTCGAACAAGGTGGCGAACCGGTCATTCCACCACGCCGTCATCGAAAGTATCAGCATGCCTACGACCGTA
TCGCCTACAAGGCCCGCTGGGGGATCGAGAGCTTCTTTGCAAAGCTCAAGCAATGGCGGCGAATCGCAACGCGCTACGACAAAGTCGCCGCCAACTTCAT
GGGATTCATCAAAATCGCCGCCATCATGCTCTGGCTCAAATGATTAAATTGTCACTGCAGCC
GACGATCAATGGGAGCGGCTGACGCCGTTAGTGCCCGGCGGTCGCAAAGGCCGGCGCGGTCCGCGTAGTGACGGACGCCGGTTCTTCGATGCGGTGTTGT
GGCTCGCCCGGTCGGGTGCGCGCTGGCGTGATCTGCCGGAAGACCGCTTCGGCCCCTATCAGACGATCAAACGGCGCTACTATCGCTGGATCGAACAGGG
CATCTTCGACAAGATTTTCGAAGCCGTCGCGATCGATCCGGATATGGAGTGGTTGTCCATCGACGCCACGGTGATCCGGGCACAAGCGCAAGCGGCAGGC
GGACGCCGAAAAAGGGGGGACCGCAAGCCCAGGCTCTCGGCCGTTCCCGCGGGGGATTTGGCAGCAAGATCCATGCAGTAGTCGATGCGCTCGGCTTGCC
GGTGCGCCTTCACCTGGGACCCGGCCAGCAGAATGATATGGCGCCTGCCTGCGATCTCGTCCGGGGGCTGCCGGCCAAGCAGGTGCTCGCCGACCGCGCC
TATGACGCCGACAGCCTGCATGACATTATTCTCGAACAAGGTGGCGAACCGGTCATTCCACCACGCCGTCATCGAAAGTATCAGCATGCCTACGACCGTA
TCGCCTACAAGGCCCGCTGGGGGATCGAGAGCTTCTTTGCAAAGCTCAAGCAATGGCGGCGAATCGCAACGCGCTACGACAAAGTCGCCGCCAACTTCAT
GGGATTCATCAAAATCGCCGCCATCATGCTCTGGCTCAAATGATTAAATTGTCACTGCAGCC
Recoding section
- Recoding by frameshift
- Frame
- Type
- Experimentally demonstrated
Stimulators :
- Shine-Dalgarno sequence :
- Secondary structure :
Recoding motif :
Protein section
ORF number : 3
ORF 1
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 402 bp | 133 aa | 80 | 481 | + | No |
Description : First part of the transposase
ORF sequence :
MDGEVLRDDQWERLTPLVPGGRKGRRGPRSDGRRFFDAVLWLARSGARWRDLPEDRFGPYQTIKRRYYRWIEQGIFDKIFEAVAIDPDMEWLSIDATVIR
AQAQAAGGRRKRGDRKPRLSAVPAGDLAARSMQ
AQAQAAGGRRKRGDRKPRLSAVPAGDLAARSMQ
Blast result :ORF 2
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 612 bp | 203 aa | 232 | 843 | + | No |
Description : Second part of the transposase
ORF sequence :
SAGRPLRPLSDDQTALLSLDRTGHLRQDFRSRRDRSGYGVVVHRRHGDPGTSASGRRTPKKGGPQAQALGRSRGGFGSKIHAVVDALGLPVRLHLGPGQQ
NDMAPACDLVRGLPAKQVLADRAYDADSLHDIILEQGGEPVIPPRRHRKYQHAYDRIAYKARWGIESFFAKLKQWRRIATRYDKVAANFMGFIKIAAIML
WLK
NDMAPACDLVRGLPAKQVLADRAYDADSLHDIILEQGGEPVIPPRRHRKYQHAYDRIAYKARWGIESFFAKLKQWRRIATRYDKVAANFMGFIKIAAIML
WLK
Blast result :ORF 3
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 764 bp | 254 aa | 80 | 843 | + | Yes |
Chemistry : DDE
ORF sequence :
MDGEVLRDDQWERLTPLVPGGRKGRRGPRSDGRRFFDAVLWLARSGARWRDLPEDRFGPYQTIKRRYYRWIEQGIFDKIFEAVAIDPDMEWLSIDATVIR
AQAQAAGGRRKKGGPQAQALGRSRGGFGSKIHAVVDALGLPVRLHLGPGQQNDMAPACDLVRGLPAKQVLADRAYDADSLHDIILEQGGEPVIPPRRHRK
YQHAYDRIAYKARWGIESFFAKLKQWRRIATRYDKVAANFMGFIKIAAIMLWLK
AQAQAAGGRRKKGGPQAQALGRSRGGFGSKIHAVVDALGLPVRLHLGPGQQNDMAPACDLVRGLPAKQVLADRAYDADSLHDIILEQGGEPVIPPRRHRK
YQHAYDRIAYKARWGIESFFAKLKQWRRIATRYDKVAANFMGFIKIAAIMLWLK
Blast result :
Comments
ISRel11 is 86% aa similar to ISCc1.
The third ORF is a putative ORFAB transposase reconstructed in silico by a possible -1 frameshift.
The third ORF is a putative ORFAB transposase reconstructed in silico by a possible -1 frameshift.
References