ISAs12
- Family ISAs1
- Group
Isoform Synonym(s)
| Accession number | Transposition | Origin | Host |
|---|---|---|---|
| HQ170513 | ND | Aeromonas salmonicida | Aeromonas salmonicida ER.1.7 |
DNA section
IS Length : 1438 bp
Ends
IR Length : 26/29
IRL : CAGGCGAGATCGTGAACATTTTGTTAAAAATTAAAAATAGTTTTTTGCCT
IRR : CAGGGTGAGAGCGTGAACATTTTGTTAAATTTCACCCAGTTTTTTGAGAC
Insertion site
| Left flank | Direct repeat | Right flank | DR Length |
|---|---|---|---|
| TGGACTCTTG | GTATTTCTGC | TGGCGCTTCT | 10 |
| CGCATTGTCG | GCAGTTCGCC | CACCACTGTG | 10 |
| TTGCACGTAG | GCTCTGGGCC | CGAGTCAGTG | 10 |
| GTTTTGATAT | ATACCAGAGT | ATGGATCTTC | 10 |
| TATGTTACTG | TCAGAATACC | TGACAAAAAC | 10 |
| CTTAGACCCA | GGGTTGCGGC | CATCCCGGCG | 10 |
| CACGCTGTTC | AGCAAGTCGG | TGTGTCAACT | 10 |
| TTCGGCAAGG | GGTCATCTAT | TACCAGTTGC | 10 |
| AGCTGCCCTC | CCACTACATA | CGTCACGACG | 10 |
DNA sequence
CAGGCGAGATCGTGAACATTTTGTTAAAAATTAAAAATAGTTTTTTGCCTACAAACAAATACTTATAGGCCTGTGATATGCAGTTAACTTTATAGTCAGG
ATCGCCTTCACACTTTTTAATCGTCACTGCACGTCATTTCAGCTCAAAAAATGCGCATGAAATCCAAAAAATGATAATTTCCATGATTTCCATAGTTAAC
AATTTGCTCACATCCTCACCTGAAATTTGACCTGGTGATCCGGCTGTGATCTCATGCTCCTTTTCAGGAGTGCTCTCATGTCTATTGATGCCGTTTTCGC
TCAGTTCTTCGACAATATTCACGACCCTCGCCAGCATGCCAAGATTTGTTATCCCTTCTACGATGTCCTGTTTCTCACCGTCTGTGCCGTGATCGGCGGG
GCTGAGGGCTGGGAAGATATTGAGGATTTCGGCGAGGTTCATCTTCCCTGGTTTCAATCCAAGGGACTGTTTAAAAACGGCATTCCTGTCCATGACACCA
TTGCCAGGATCATCTCCGGCATCAAACCAGAGCCGTTTCAGGCCGCCTTTGTGCGGTGGACTCAAGCCATTAACCAGCACACCGATGGCGCCCTGGTCGC
TATCGATGGCAAAACCCTGCGCAGTTCCTATGACCGTGAGGATCGAACGTCGACCATTCACATGGTCAGTGCCTATGCCGCCGCCAATAAGCTGGTGCTC
GGACAGATAAAAACCGACGCCAAGTCCAATGAAATTACCGCTATTCCCGAGTTGCCGGCGCTGCTCGACATCAAAGGCTGCCTGGTATCCATCGATGCGA
TGGGGTGCCAGACCGAGATCGCCGAGACCATCCTTCAGCAAGGTGGCGATTACTTATTGGCCGTCAAAGGCAACCAACCCAAGTTGTTCGAGGCGGTTCG
ACAGGCCCTCGCCCCGCTGGCGGCCACCCCGTTATGCGCAGAGACGATGACGCTTGAGAAGGCTCATGGCCGCATCGATGGTCGTGAGTACCATGTCATG
GCGGCGGGCGAGTTGGCCGCCCAGTTTCCTCATTGGAAACAGTTGCACAGCATTGGCGTGGCCATCTCCTATCGCGTTGAAAATATGCAAAAAATTACCA
TAGAGCAGCGTTATTTCATCAGTTCCAAAGCGTTGGTGCAGGATGAGTTTGCGCGAGCGGTGCGTGGGCACTGGGCCATCGAAAATAGCCTGCACTGGGT
GCTGGACGTCACGATGGGCGAGGATGATTGCCCGATTTATCGCGGTGATGCCGCAGAGATTTTGGCGTGCATCCGGCACATGGGGCTGAACATGCTCAGG
GCCGAGACTTCACGCAAGGCGAGCGTAAGACGGAAGCAAAAAATCGCAGGAATGAGCAGTGAATATCTGGAAACGGTACTCAATGCGGGTCTCAAAAAAC
TGGGTGAAATTTAACAAAATGTTCACGCTCTCACCCTG
ATCGCCTTCACACTTTTTAATCGTCACTGCACGTCATTTCAGCTCAAAAAATGCGCATGAAATCCAAAAAATGATAATTTCCATGATTTCCATAGTTAAC
AATTTGCTCACATCCTCACCTGAAATTTGACCTGGTGATCCGGCTGTGATCTCATGCTCCTTTTCAGGAGTGCTCTCATGTCTATTGATGCCGTTTTCGC
TCAGTTCTTCGACAATATTCACGACCCTCGCCAGCATGCCAAGATTTGTTATCCCTTCTACGATGTCCTGTTTCTCACCGTCTGTGCCGTGATCGGCGGG
GCTGAGGGCTGGGAAGATATTGAGGATTTCGGCGAGGTTCATCTTCCCTGGTTTCAATCCAAGGGACTGTTTAAAAACGGCATTCCTGTCCATGACACCA
TTGCCAGGATCATCTCCGGCATCAAACCAGAGCCGTTTCAGGCCGCCTTTGTGCGGTGGACTCAAGCCATTAACCAGCACACCGATGGCGCCCTGGTCGC
TATCGATGGCAAAACCCTGCGCAGTTCCTATGACCGTGAGGATCGAACGTCGACCATTCACATGGTCAGTGCCTATGCCGCCGCCAATAAGCTGGTGCTC
GGACAGATAAAAACCGACGCCAAGTCCAATGAAATTACCGCTATTCCCGAGTTGCCGGCGCTGCTCGACATCAAAGGCTGCCTGGTATCCATCGATGCGA
TGGGGTGCCAGACCGAGATCGCCGAGACCATCCTTCAGCAAGGTGGCGATTACTTATTGGCCGTCAAAGGCAACCAACCCAAGTTGTTCGAGGCGGTTCG
ACAGGCCCTCGCCCCGCTGGCGGCCACCCCGTTATGCGCAGAGACGATGACGCTTGAGAAGGCTCATGGCCGCATCGATGGTCGTGAGTACCATGTCATG
GCGGCGGGCGAGTTGGCCGCCCAGTTTCCTCATTGGAAACAGTTGCACAGCATTGGCGTGGCCATCTCCTATCGCGTTGAAAATATGCAAAAAATTACCA
TAGAGCAGCGTTATTTCATCAGTTCCAAAGCGTTGGTGCAGGATGAGTTTGCGCGAGCGGTGCGTGGGCACTGGGCCATCGAAAATAGCCTGCACTGGGT
GCTGGACGTCACGATGGGCGAGGATGATTGCCCGATTTATCGCGGTGATGCCGCAGAGATTTTGGCGTGCATCCGGCACATGGGGCTGAACATGCTCAGG
GCCGAGACTTCACGCAAGGCGAGCGTAAGACGGAAGCAAAAAATCGCAGGAATGAGCAGTGAATATCTGGAAACGGTACTCAATGCGGGTCTCAAAAAAC
TGGGTGAAATTTAACAAAATGTTCACGCTCTCACCCTG
Protein section
ORF number : 1
ORF 1
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 1137 bp | 378 aa | 278 | 1414 | + | No |
Chemistry : Unknow
ORF sequence :
MSIDAVFAQFFDNIHDPRQHAKICYPFYDVLFLTVCAVIGGAEGWEDIEDFGEVHLPWFQSKGLFKNGIPVHDTIARIISGIKPEPFQAAFVRWTQAINQ
HTDGALVAIDGKTLRSSYDREDRTSTIHMVSAYAAANKLVLGQIKTDAKSNEITAIPELPALLDIKGCLVSIDAMGCQTEIAETILQQGGDYLLAVKGNQ
PKLFEAVRQALAPLAATPLCAETMTLEKAHGRIDGREYHVMAAGELAAQFPHWKQLHSIGVAISYRVENMQKITIEQRYFISSKALVQDEFARAVRGHWA
IENSLHWVLDVTMGEDDCPIYRGDAAEILACIRHMGLNMLRAETSRKASVRRKQKIAGMSSEYLETVLNAGLKKLGEI
HTDGALVAIDGKTLRSSYDREDRTSTIHMVSAYAAANKLVLGQIKTDAKSNEITAIPELPALLDIKGCLVSIDAMGCQTEIAETILQQGGDYLLAVKGNQ
PKLFEAVRQALAPLAATPLCAETMTLEKAHGRIDGREYHVMAAGELAAQFPHWKQLHSIGVAISYRVENMQKITIEQRYFISSKALVQDEFARAVRGHWA
IENSLHWVLDVTMGEDDCPIYRGDAAEILACIRHMGLNMLRAETSRKASVRRKQKIAGMSSEYLETVLNAGLKKLGEI
Blast result :
Comments
ISAs12 was found in Aeromonas salmonicida strain ER.1.7, disrupting a putative gene cassette with unknown function in a class 1 integron.
ISAs12 is 71% aa similar to ISSod22.
File updated the March 03 2015 with the information provided by F. Pfeiffer.
ISAs12 is 71% aa similar to ISSod22.
File updated the March 03 2015 with the information provided by F. Pfeiffer.
References
1] Moura,A., Pereira,C., Henriques,I. and Correia,A. (2012) Res Microbiol. Feb;163(2):92-100
2] Moura,A. (2010) Direct submission.
2] Moura,A. (2010) Direct submission.